Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos

ilustraciones, diagramas

Autores:
Montoya Páez, Juan David
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/83352
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/83352
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología
590 - Animales::599 - Mamíferos
Criopreservacion de órganos, tejidos. etc.
Conservación del semen
Semen - Criopreservación
Cryopreservation of organs, tissues, etc.
Semen conservation
Burro
Asno
Semen
Plasma seminal
Crioprotector permeable
Crioprotector no permeable
Calidad seminal
Capacidad antioxidante
Especies reactivas de oxígeno
Seminal plasma
Permeable cryoprotectant
Non-permeable cryoprotectant
Seminal quality
Antioxidant capacity
Reactive oxygen species
Rights
openAccess
License
Reconocimiento 4.0 Internacional
id UNACIONAL2_e45aae6ccb884ab0859707c62d99633d
oai_identifier_str oai:repositorio.unal.edu.co:unal/83352
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
dc.title.translated.eng.fl_str_mv Evaluation of factors associated with cryotolerance, redox state and fertilizing capacity of asinine semen, aimed at the conservation of colombian creole donkeys (Equus asinus)
title Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
spellingShingle Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
570 - Biología
590 - Animales::599 - Mamíferos
Criopreservacion de órganos, tejidos. etc.
Conservación del semen
Semen - Criopreservación
Cryopreservation of organs, tissues, etc.
Semen conservation
Burro
Asno
Semen
Plasma seminal
Crioprotector permeable
Crioprotector no permeable
Calidad seminal
Capacidad antioxidante
Especies reactivas de oxígeno
Seminal plasma
Permeable cryoprotectant
Non-permeable cryoprotectant
Seminal quality
Antioxidant capacity
Reactive oxygen species
title_short Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
title_full Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
title_fullStr Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
title_full_unstemmed Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
title_sort Evaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianos
dc.creator.fl_str_mv Montoya Páez, Juan David
dc.contributor.advisor.none.fl_str_mv Restrepo Betancur, Giovanni
Usuga Suarez, Alexandra
dc.contributor.author.none.fl_str_mv Montoya Páez, Juan David
dc.contributor.researcher.none.fl_str_mv Úsuga Suárez, Alexandra
Restrepo Betancur, Giovanni
Rojano, Benjamín Alberto
Restrepo Betancur, Giovanni
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Investigación en Biotecnología Animal GIBA
Química de los Productos Naturales y los Alimentos
dc.contributor.orcid.spa.fl_str_mv Montoya Páez, Juan David [0000-0002-9677-0426]
dc.contributor.cvlac.spa.fl_str_mv Montoya Páez, Juan David
dc.contributor.researchgate.spa.fl_str_mv Montoya Páez, Juan David
dc.subject.ddc.spa.fl_str_mv 570 - Biología
590 - Animales::599 - Mamíferos
topic 570 - Biología
590 - Animales::599 - Mamíferos
Criopreservacion de órganos, tejidos. etc.
Conservación del semen
Semen - Criopreservación
Cryopreservation of organs, tissues, etc.
Semen conservation
Burro
Asno
Semen
Plasma seminal
Crioprotector permeable
Crioprotector no permeable
Calidad seminal
Capacidad antioxidante
Especies reactivas de oxígeno
Seminal plasma
Permeable cryoprotectant
Non-permeable cryoprotectant
Seminal quality
Antioxidant capacity
Reactive oxygen species
dc.subject.lemb.spa.fl_str_mv Criopreservacion de órganos, tejidos. etc.
Conservación del semen
Semen - Criopreservación
dc.subject.lemb.eng.fl_str_mv Cryopreservation of organs, tissues, etc.
Semen conservation
dc.subject.proposal.spa.fl_str_mv Burro
Asno
Semen
Plasma seminal
Crioprotector permeable
Crioprotector no permeable
Calidad seminal
Capacidad antioxidante
Especies reactivas de oxígeno
dc.subject.proposal.eng.fl_str_mv Seminal plasma
Permeable cryoprotectant
Non-permeable cryoprotectant
Seminal quality
Antioxidant capacity
Reactive oxygen species
description ilustraciones, diagramas
publishDate 2022
dc.date.issued.none.fl_str_mv 2022-11-04
dc.date.accessioned.none.fl_str_mv 2023-02-07T16:01:10Z
dc.date.available.none.fl_str_mv 2023-02-07T16:01:10Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/83352
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/83352
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv RedCol
LaReferencia
dc.relation.references.spa.fl_str_mv Aebi, H. (1984). Catalase in vitro. In Antioxidant Enzymes of Seminal Plasma International Braz J Urol (Vol. 34, Issue 4, pp. 121–126). https://doi.org/10.1016/S0076-6879(84)05016-3
Agarwal, A., Gupta, S., & Sikka, S. (2006). The role of free radicals and antioxidants in reproduction. Current Opinion in Obstetrics & Gynecology, 18(3), 325–332. https://doi.org/10.1097/01.GCO.0000193003.58158.4E
Aitken, J., & Fisher, H. (1994). Reactive oxygen species generation and human spermatozoa: The balance of benefit and risk. BioEssays, 16(4), 259–267. https://doi.org/10.1002/BIES.950160409
Arts, M. J. T. ., Sebastiaan Dallinga, J., Voss, H.-P., Haenen, G. R. M. ., & Bast, A. (2004). A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chemistry, 88(4), 567–570. https://doi.org/10.1016/j.foodchem.2004.02.008
Balao da Silva, C. M., Spinaci, M., Bucci, D., Giaretta, E., Peña, F. J., Mari, G., & Galeati, G. (2013). Effect of sex sorting on stallion spermatozoa: Heterologous oocyte binding, tyrosine phosphorylation and acrosome reaction assay. Animal Reproduction Science, 141(1–2), 68–74. https://doi.org/10.1016/J.ANIREPROSCI.2013.07.008
Ball, B. A. (2008). Oxidative stress, osmotic stress and apoptosis: Impacts on sperm function and preservation in the horse. Animal Reproduction Science, 107(3–4), 257–267. https://doi.org/10.1016/J.ANIREPROSCI.2008.04.014
Ball, B. A., Vo, A. T., & Baumber, J. (2001). Generation of reactive oxygen species by equine spermatozoa. American Journal of Veterinary Research, 62(4), 508–515. https://doi.org/10.2460/AJVR.2001.62.508
Barth, A. D., & Oko, R. J. (1989). Abnormal morphology of bovine spermatozoa (1st ed.). Iowa State University Press.
Benítez-Estrada, A., Villanueva-Sánchez, J., González-Rosendo, G., Alcántar-Rodríguez, V. E., Puga-Díaz, R., Quintero-Gutiérrez, A. G., Benítez-Estrada, A., Villanueva-Sánchez, J., González-Rosendo, G., Alcántar-Rodríguez, V. E., Puga-Díaz, R., & Quintero-Gutiérrez, A. G. (2020). Determinación de la capacidad antioxidante total de alimentos y plasma humano por fotoquimioluminiscencia: Correlación con ensayos fluorométricos (ORAC) y espectrofotométricos (FRAP). TIP. Revista Especializada En Ciencias Químico-Biológicas, 23, 1–9. https://doi.org/10.22201/FESZ.23958723E.2020.0.244
Bhagat, S., Soni, A., Mishra, S., Santra, A., Sonkar, N., & Verma, U. (2020). Body condition scoring of Equine: Short note. ~ 19 ~ The Pharma Innovation Journal, 9(3), 19–20. http://www.thepharmajournal.com
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Brahmkshtri, B. P., Edwin, M. J., John, M. C., Nainar, A. M., & Krishnan, A. R. (1999). Relative efficacy of conventional sperm parameters and sperm penetration bioassay to assess bull fertility in vitro. Animal Reproduction Science, 54(3), 159–168. https://doi.org/10.1016/S0378-4320(98)00108-0
Bresnahan, D. R., Lupole, R. E., Stilz, C. R., & Carnevale, E. M. (2022). Effect of Mare Age on Transcript Abundance of Connexins-37 and -43, Zona Pellucida Proteins, and Sperm Binding. Journal of Equine Veterinary Science, 108, 103796. https://doi.org/10.1016/J.JEVS.2021.103796
Brito, L. F. C., Greene, L. M., Kelleman, A., Knobbe, M., & Turner, R. (2011). Effect of method and clinician on stallion sperm morphology evaluation. Theriogenology. https://doi.org/10.1016/j.theriogenology.2011.04.007
Bucci, D., Giaretta, E., Spinaci, M., Rizzato, G., Isani, G., Mislei, B., Mari, G., Tamanini, C., & Galeati, G. (2016). Characterization of alkaline phosphatase activity in seminal plasma and in fresh and frozen–thawed stallion spermatozoa. Theriogenology, 85(2), 288-295.e2. https://doi.org/10.1016/J.THERIOGENOLOGY.2015.09.007
Carver, D. A., & Ball, B. A. (2002). Lipase activity in stallion seminal plasma and the effect of lipase on stallion spermatozoa during storage at 5 °C. Theriogenology, 58(8), 1587–1595. https://doi.org/10.1016/S0093-691X(02)01049-X
Catalán, J., Yánez-Ortiz, I., Tvarijonaviciute, A., González-Arostegui, L. G., Rubio, C. P., Yeste, M., Miró, J., & Barranco, I. (2022). Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance. Antioxidants, 11(2), 417. https://doi.org/10.3390/ANTIOX11020417/S1
Cecchini Gualandi, S., Giangaspero, B., Di Palma, T., Macchia, G., Carluccio, A., & Boni, R. (2021). Oxidative profile and protease regulator potential to predict sperm functionality in donkey (Equus asinus). Scientific Reports 2021 11:1, 11(1), 1–10. https://doi.org/10.1038/s41598-021-99972-9
Cohen, D. J., Maldera, J. A., Weigel Muñoz, M., Ernesto, J. I., Vasen, G., & Cuasnicu, P. S. (2011). Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization. Biological Research, 44(2), 135–138. https://doi.org/10.4067/S0716-97602011000200004
Consuegra, C., Crespo, F., Dorado, J., Diaz-Jimenez, M., Pereira, B., Sánchez-Calabuig, M. J., Beltrán-Breña, P., Pérez-Cerezales, S., Rizos, D., & Hidalgo, M. (2020). Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures. Animal Reproduction Science, 223, 106627. https://doi.org/10.1016/J.ANIREPROSCI.2020.106627
Contri, A., Gloria, A., Robbe, D., Sfirro, M. P., & Carluccio, A. (2012). Effect of sperm concentration on characteristics of frozen-thawed semen in donkeys. Animal Reproduction Science, 136(1–2), 74–80. https://doi.org/10.1016/J.ANIREPROSCI.2012.10.022
Dacheux, J. L., Castella, S., Gatti, J. L., & Dacheux, F. (2005). Epididymal cell secretory activities and the role of proteins in boar sperm maturation. Theriogenology, 63(2), 319–341. https://doi.org/10.1016/J.THERIOGENOLOGY.2004.09.015
Dominguez, E. M., Moreno-Irusta, A., Rodriguez, M. B., Salamone, D. F., de Arruda, R. P., Losinno, L., & Giojalas, L. C. (2020). Chemotactic selection of frozen-thawed stallion sperm improves sperm quality and heterologous binding to oocytes. Animal Reproduction Science, 221, 106582. https://doi.org/10.1016/J.ANIREPROSCI.2020.106582
Gamboa, S., Rodrigues, A. S., Henriques, L., Batista, C., & Ramalho-Santos, J. (2010). Seasonal functional relevance of sperm characteristics in equine spermatozoa. Theriogenology, 73(7), 950–958. https://doi.org/10.1016/J.THERIOGENOLOGY.2009.11.023
Giesecke, K., Sieme, H., & Distl, O. (2010). Infertility and candidate gene markers for fertility in stallions: A review. The Veterinary Journal, 185(3), 265–271. https://doi.org/10.1016/J.TVJL.2009.07.024
Graham, J. K., & Mocé, E. (2005). Fertility evaluation of frozen/thawed semen. Theriogenology, 64(3), 492–504. https://doi.org/10.1016/J.THERIOGENOLOGY.2005.05.006
Guthrie, H. D., & Welch, G. R. (2006). Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry1. Journal of Animal Science, 84(8), 2089–2100. https://doi.org/10.2527/jas.2005-766
Hamann, H., Jude, R., Sieme, H., Mertens, U., Töpfer-Petersen, E., Distl, O., & Leeb, T. (2007). A polymorphism within the equine CRISP3 gene is associated with stallion fertility in Hanoverian warmblood horses. Animal Genetics, 38(3), 259–264. https://doi.org/10.1111/J.1365-2052.2007.01594.X
Härtlová, H., Rajmon, R., Krontorádová, I., Mamica, J., Zita, L., Klabanová, P., & Černocký, A. (2013). Semen quality, lipid peroxidation, and seminal plasma antioxidant status in horses with different intensities of physical exercise. Acta Veterinaria Brno, 82(1), 31–35. https://doi.org/10.2754/avb201382010031
Hasegawa, A., & Koyama, K. (2007). Contribution of zona proteins to oocyte growth. Society of Reproduction and Fertility Supplement, 63, 229–235. https://pubmed.ncbi.nlm.nih.gov/17566276/
Kankofer, M., Kolm, G., Aurich, J., & Aurich, C. (2005). Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5°C. Theriogenology, 63(5), 1354–1365. https://doi.org/10.1016/J.THERIOGENOLOGY.2004.07.005
Kareskoski, M., & Katila, T. (2008). Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity. Animal Reproduction Science, 107(3–4), 249–256. https://doi.org/10.1016/J.ANIREPROSCI.2008.04.013
Len, J., Beehan, D., Eilts, B., Ebrahimie, E., & Lyle, S. (2020). Stallion Sperm Integrity After Centrifugation to Reduce Seminal Plasma Concentration and Cool Storage for 4 days. Journal of Equine Veterinary Science, 85. https://doi.org/10.1016/J.JEVS.2019.102819
Maeda, T., Nishida, J., & Nakanishi, Y. (1999). Expression pattern, subcellular localization and structure--function relationship of rat Tpx-1, a spermatogenic cell adhesion molecule responsible for association with Sertoli cells. Development, Growth & Differentiation, 41(6), 715–722. https://doi.org/10.1046/J.1440-169X.1999.00470.X
Magdaleno, L., Gasset, M., Varea, J., Schambony, A. M., Urbanke, C., Raida, M., Töpfer-Petersen, E., & Calvete, J. J. (1997). Biochemical and conformational characterisation of HSP-3, a stallion seminal plasma protein of the cysteine-rich secretory protein (CRISP) family. FEBS Letters, 420(2–3), 179–185. https://doi.org/10.1016/S0014-5793(97)01514-7
Marklund, S., & Marklund, G. (1974). Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase. European Journal of Biochemistry, 47(3), 469–474. https://doi.org/10.1111/J.1432-1033.1974.TB03714.X
Michael, A., Alexopoulos, C., Pontiki, E., Hadjipavlou-Litina, D., Saratsis, P., & Boscos, C. (2007). Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology, 68(2), 204–212. https://doi.org/10.1016/J.THERIOGENOLOGY.2007.04.053
Miragaya, M. H., Neild, D. M., & Alonso, A. E. (2018). A Review of Reproductive Biology and Biotechnologies in Donkeys. Journal of Equine Veterinary Science, 65, 55–61. https://doi.org/10.1016/J.JEVS.2017.12.005
Miró, J, Taberner, E., Rivera, M., Peña, A., Medrano, A., Rigau, T., & Peñalba, A. (2009). Effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey semen. Theriogenology, 72(8), 1017–1022. https://doi.org/10.1016/j.theriogenology.2009.06.012
Miró, Jordi, Vilés, K., García, W., Jordana, J., & Yeste, M. (2013). Effect of donkey seminal plasma on sperm movement and sperm-polymorphonuclear neutrophils attachment in vitro. Animal Reproduction Science, 140, 164–172. https://doi.org/10.1016/j.anireprosci.2013.06.007
Monteiro, G. A., Papa, F. O., Zahn, F. S., Dellaqua, J. A., Melo, C. M., Maziero, R. R. D., Avanzi, B. R., Alvarenga, M. A., & Guasti, P. N. (2011). Cryopreservation and fertility of ejaculated and epididymal stallion sperm. Animal Reproduction Science, 127(3–4), 197–201. https://doi.org/10.1016/j.anireprosci.2011.08.002
Monteiro, R. A., Cunha, R. M., Guerra, M. M. P., de Almeida, V. M., Peña-Alfaro, C. E., & Silva, S. V. (2022). Use of Equine Sperm Cryopreservation Techniques as a Conservation Method of Donkey Germplasm. Journal of Equine Veterinary Science, 111, 103888. https://doi.org/10.1016/j.jevs.2022.103888
Montoya, J. D., Rojano, B., & Restrepo, G. (2017). Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación. Archivos de Zootecnia, 66(255), 333. https://doi.org/10.21071/az.v66i255.2508
Montoya Páez, J. D., Rojano, B. A., Restrepo Betancur, G., Montoya Páez, J. D., Rojano, B. A., & Restrepo Betancur, G. (2017). Suplementación con plasma seminal y relación de sus componentes con la calidad de semen congelado-descongelado de asnos (Equus asinus). Revista Mexicana de Ciencias Pecuarias, 8(3), 233–242. https://doi.org/10.22319/RMCP.V8I3.4499
Muñoz, M. W., Ernesto, J. I., Bluguermann, C., Busso, D., Battistone, M. A., Cohen, D. J., & Cuasnicú, P. S. (2012). Evaluation of testicular sperm CRISP2 as a potential target for contraception. Journal of Andrology, 33(6), 1360–1370. https://doi.org/10.2164/JANDROL.112.016725
Neild, D., Chaves, G., Flores, M., Mora, N., Beconi, M., & Agüero, A. (1999). Hypoosmotic test in equine spermatozoa. Theriogenology. https://doi.org/10.1016/S0093-691X(99)00021-7
Neuhauser, S., Gösele, P., & Handler, J. (2019). Postthaw Addition of Autologous Seminal Plasma Improves Sperm Motion Characteristics in Fair and Poor Freezer Stallions. Journal of Equine Veterinary Science, 72, 117–123. https://doi.org/10.1016/J.JEVS.2018.10.028
Nouri, H., Towhidi, A., Zhandi, M., & Sadeghi, R. (2013). The effects of centrifuged egg yolk used with INRA plus soybean lecithin extender on semen quality to freeze miniature caspian horse semen. Journal of Equine Veterinary Science, 33(12), 1050–1053. https://doi.org/10.1016/J.JEVS.2013.03.184
Ortega-Ferrusola, C., Johannisson, A., Peña Vega, F. J., Tapia, J. A., Rodriguez-Martinez, H., Dalin, A. M., & Morrell, J. M. (2011). Effect of Different Extenders and Seminal Plasma on the Susceptibility of Equine Spermatozoa to Lipid Peroxidation After Single-Layer Centrifugation, Through Androcoll-E. Journal of Equine Veterinary Science, 31(7), 411–416. https://doi.org/10.1016/J.JEVS.2011.01.010
Ortiz, I., Dorado, J., Acha, D., Gálvez, M. J., Urbano, M., & Hidalgo, M. (2015). Colloid single-layer centrifugation improves post-thaw donkey (Equus asinus) sperm quality and is related to ejaculate freezability. Reproduction, Fertility and Development, 27(2), 332. https://doi.org/10.1071/RD13246
Ou, B., Hampsch-Woodill, M., & Prior, R. L. (2001). Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe. Journal of Agricultural and Food Chemistry, 49(10), 4619–4626. https://doi.org/10.1021/jf010586o
Papa, F. O., Melo, C. M., Fioratti, E. G., Dell’Aqua, J. A., Zahn, F. S., & Alvarenga, M. A. (2008). Freezing of stallion epididymal sperm. Animal Reproduction Science, 107(3–4), 293–301. https://doi.org/10.1016/J.ANIREPROSCI.2008.05.003
Papas, M., Catalan, J., Barranco, I., Arroyo, L., Bassols, A., Yeste, M., & Miró, J. (2020). Total and specific activities of superoxide dismutase (SOD) in seminal plasma are related with the cryotolerance of jackass spermatozoa. Cryobiology, 92, 109–116. https://doi.org/10.1016/J.CRYOBIOL.2019.11.043
Pontiki, E., & Hadjipavlou-Litina, D. (2006). Antioxidant and Anti-Inflammatory Activity of Aryl-Acetic and Hydroxamic Acids as Novel Lipoxygenase Inhibitors. Medicinal Chemistry, 2(3), 251–264. https://doi.org/10.2174/157340606776930763
Portus, B. J., Reilas, T., & Katila, T. (2005). Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares. Equine Veterinary Journal, 37(6), 515–519. https://doi.org/10.2746/042516405775314844
Rodríguez-Martínez, H., Kvist, U., Ernerudh, J., Sanz, L., & Calvete, J. J. (2011). Seminal Plasma Proteins: What Role Do They Play? American Journal of Reproductive Immunology, 66(SUPPL. 1), 11–22. https://doi.org/10.1111/j.1600-0897.2011.01033.x
Schambony, A., Gentzel, M., Wolfes, H., Raida, M., Neumann, U., & Töpfer-Petersen, E. (1998). Equine CRISP-3: primary structure and expression in the male genital tract. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1387(1–2), 206–216. https://doi.org/10.1016/S0167-4838(98)00122-8
Serres, C., Rodriguez, A., Alvarez, A. L., Santiago, I., Gabriel, J., Gómez-Cuétara, C., & Mateos, E. (2002). Effect of centrifugation and temperature on the motility and plasma membrane integrity of Zamorano-Leonés donkey semen. Theriogenology, 58(2–4), 329–332. https://doi.org/10.1016/S0093-691X(02)00876-2
Shamsi, M. B., Venkatesh, S., Tanwar, M., Talwar, P., Sharma, R. K., Dhawan, A., Kumar, R., Gupta, N. P., Malhotra, N., Singh, N., Mittal, S., & Dada, R. (2009). DNA integrity and semen quality in men with low seminal antioxidant levels. Mutation Research, 665(1–2), 29–36. https://doi.org/10.1016/J.MRFMMM.2009.02.017
Töpfer-Petersen, E., Ekhlasi-Hundrieser, M., Kirchhoff, C., Leeb, T., & Sieme, H. (2005). The role of stallion seminal proteins in fertilisation. Animal Reproduction Science, 89(1–4), 159–170. https://doi.org/10.1016/J.ANIREPROSCI.2005.06.018
Troedsson, M. H., Lee, C. S., Franklin, R. D., & Crabo, B. G. (2000). The role of seminal plasma in post-breeding uterine inflammation. Journal of Reproduction and Fertility. Supplement, 56, 341–349. https://europepmc.org/article/med/20681146
Usuga, A., Rojano, B. A., Restrepo, G., Usuga, A., Rojano, B. A., & Restrepo, G. (2018). Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen. Reproduction, Fertility and Development, 30(3), 563–569. https://doi.org/10.1071/RD17044
Usuga, A., Rojano, B., & Restrepo, G. (2020). Lyophilized seminal plasma can improve stallion semen freezability. Indian Journal of Animal Sciences, 90(2), 171–175. https://doi.org/10.13140/RG.2.2.25756.03206
Vilés, K., Rabanal, R., Rodríguez-Prado, M., & Miró, J. (2013). Effect of ketoprofen treatment on the uterine inflammatory response after AI of jennies with frozen semen. Theriogenology, 79, 1019–1026. https://doi.org/10.1016/j.theriogenology.2013.01.006
Waheed, M. M., El-Bahr, S. M., & Al-haider, A. K. (2013). Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse. Journal of Equine Veterinary Science, 33(9), 705–709. https://doi.org/10.1016/J.JEVS.2012.11.006
Wittayarat, M., Pukazhenthi, B. S., Tipkantha, W., Techakumphu, M., Srisuwatanasagul, S., & Panyaboriban, S. (2021). CRISP protein expression in semen of the endangered Malayan tapir (Tapirus indicus). Theriogenology, 172, 106–115. https://doi.org/10.1016/J.THERIOGENOLOGY.2021.06.005
Wood, P. L., Scoggin, K., Ball, B. A., Troedsson, M. H., & Squires, E. L. (2016). Lipidomics of equine sperm and seminal plasma: Identification of amphiphilic (O-acyl)-ω-hydroxy-fatty acids. Theriogenology, 86(5), 1212–1221. https://doi.org/10.1016/J.THERIOGENOLOGY.2016.04.012
Yoshida, M. (2000). Conservation of sperms: Current status and new trends. Animal Reproduction Science, 60–61, 349–355. https://doi.org/10.1016/S0378-4320(00)00125-1
Bottrel, M., Acha, D., Ortiz, I., Hidalgo, M., Gósalvez, J., Camisão, J., & Dorado, J. (2018). Cryoprotective effect of glutamine, taurine, and proline on post-thaw semen quality and DNA integrity of donkey spermatozoa. Animal Reproduction Science, 189, 128–135. https://doi.org/10.1016/J.ANIREPROSCI.2017.12.021
Burden, F., & Thiemann, A. (2015). Donkeys Are Different. Journal of Equine Veterinary Science, 35(5), 376–382. https://doi.org/10.1016/J.JEVS.2015.03.005
Camillo, F., Rota, A., Biagini, L., Tesi, M., Fanelli, D., & Panzani, D. (2018). The Current Situation and Trend of Donkey Industry in Europe. Journal of Equine Veterinary Science, 65, 44–49. https://doi.org/10.1016/J.JEVS.2017.11.008
Contri, A., Gloria, A., Robbe, D., Sfirro, M. P., & Carluccio, A. (2012). Effect of sperm concentration on characteristics of frozen-thawed semen in donkeys. Animal Reproduction Science, 136(1–2), 74–80. https://doi.org/10.1016/J.ANIREPROSCI.2012.10.022
Food and Agricultural Organization (FAO). Commission on genetic resources for food and agriculture: Status and trends of Animal Genetic Resources. Disponible en: http://www.fao.org/3/a-mm278e.pdf. 2018
Guasti, P. N., Monteiro, G. A., & Papa, F. O. (2012). Componentes do plasma seminal e sua influência sobre a criopreservação e fertilidade de espermatozóides equinos. Veterinária e Zootecnia. https://repositorio.unesp.br/handle/11449/141249?show=full
Kareskoski, M., & Katila, T. (2008). Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity. Animal Reproduction Science, 107(3–4), 249–256. https://doi.org/10.1016/J.ANIREPROSCI.2008.04.013
Leeb, T., Sieme, H., & Töpfer-Petersen, E. (2005). Genetic markers for stallion fertility--lessons from humans and mice. Animal Reproduction Science, 89(1–4), 21–29. https://doi.org/10.1016/J.ANIREPROSCI.2005.06.021
Macías García, B., Ortega Ferrusola, C., Aparicio, I. M., Miró-Morán, A., Morillo Rodriguez, A., Gallardo Bolaños, J. M., González Fernández, L., Balao da Silva, C. M., Rodríguez Martínez, H., Tapia, J. A., & Peña, F. J. (2012). Toxicity of glycerol for the stallion spermatozoa: effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential. Theriogenology, 77(7), 1280–1289. https://doi.org/10.1016/J.THERIOGENOLOGY.2011.10.033
Madison, R. J., Evans, L. E., & Youngs, C. R. (2013). The Effect of 2-Hydroxypropyl-β-Cyclodextrin on Post-Thaw Parameters of Cryopreserved Jack and Stallion Semen. Journal of Equine Veterinary Science, 33(4), 272–278. https://doi.org/10.1016/j.jevs.2012.07.021
Ministerio De Agricultura Y Desarrollo Rural De Colombia. 2020. Cadena equina, asnal y mular. Documento disponible en: https://sioc.minagricultura.gov.co/Equino/Documentos/005%20-%20Documentos%20T%C3%A9cnicos/Diagnostico%20Cadena%20Equina,%20Asnal%20y%20Mular.pdf. Consultado el 10 de abril de 2022
Miró, Jordi, Viles, K., Fernandez, M., Fabregas, N., Soares, J., & Garcia, W. (2011). Induced acute endometritis by frozen semen insemination in donkey | Request PDF. Reproduction in Domestic Animals. https://www.researchgate.net/publication/313077361_Induced_acute_endometritis_by_frozen_semen_insemination_in_donkey
Monteiro, R. A., Cunha, R. M., Guerra, M. M. P., de Almeida, V. M., Peña-Alfaro, C. E., & Silva, S. V. (2022). Use of Equine Sperm Cryopreservation Techniques as a Conservation Method of Donkey Germplasm. Journal of Equine Veterinary Science, 111, 103888. https://doi.org/10.1016/j.jevs.2022.103888
Oliveira, J. V. de, Oliveira, P. V. de L. F., Melo e Oña, C. M., Guasti, P. N., Monteiro, G. A., Sancler da Silva, Y. F. R., Papa, P. de M., Alvarenga, M. A., Dell’Aqua Junior, J. A., & Papa, F. O. (2016). Strategies to improve the fertility of fresh and frozen donkey semen. Theriogenology, 85(7), 1267–1273. https://doi.org/10.1016/j.theriogenology.2015.12.010
Panzani, D., Rota, A., Tesi, M., Fanelli, D., & Camillo, F. (2018). Update on Donkey Embryo Transfer and Cryopreservation. Journal of Equine Veterinary Science, 65, 50–54. https://doi.org/10.1016/J.JEVS.2017.11.012
Papa, F. O., Melo, C. M., Fioratti, E. G., Dell’Aqua, J. A., Zahn, F. S., & Alvarenga, M. A. (2008). Freezing of stallion epididymal sperm. Animal Reproduction Science, 107(3–4), 293–301. https://doi.org/10.1016/J.ANIREPROSCI.2008.05.003
Revista Semana. El trágico destino de los burros que se desaparecen en la Costa. Tomado de https://www.semana.com/nacion/articulo/desaparecen-burros-en-lacosta/519103. 2017
Rodriguez-Martinez, H., & Wallgren, M. (2011). Advances in Boar Semen Cryopreservation. Veterinary Medicine International, 2011. https://doi.org/10.4061/2011/396181
Rossel, S., Marshall, F., Peters, J., Pilgram, T., Adams, M. D., & O’Connor, D. (2008). Domestication of the donkey: Timing, processes, and indicators. Proceedings of the National Academy of Sciences of the United States of America, 105(10), 3715–3720. https://doi.org/10.1073/PNAS.0709692105/SUPPL_FILE/INDEX.HTML
Rota, A., Panzani, D., Sabatini, C., & Camillo, F. (2012). Donkey jack (Equus asinus) semen cryopreservation: Studies of seminal parameters, post breeding inflammatory response, and fertility in donkey jennies. Theriogenology, 78(8), 1846–1854. https://doi.org/10.1016/J.THERIOGENOLOGY.2012.07.015
Rota, Alessandra, Puddu, B., Sabatini, C., Panzani, D., Lainé, A. L., & Camillo, F. (2018). Reproductive parameters of donkey jacks undergoing puberty. Animal Reproduction Science, 192, 119–125. https://doi.org/10.1016/J.ANIREPROSCI.2018.02.02
Serres, C., Rodriguez, A., Alvarez, A. L., Santiago, I., Gabriel, J., Gómez-Cuétara, C., & Mateos, E. (2002). Effect of centrifugation and temperature on the motility and plasma membrane integrity of Zamorano-Leonés donkey semen. Theriogenology, 58(2–4), 329–332. https://doi.org/10.1016/S0093-691X(02)00876-2
The Donkey Sanctuary. What is ejiao? Tomado de www.thedonkeysanctuary.org.uk. 2017
Vidament, M., Vincent, P., Martin, F. X., Magistrini, M., & Blesbois, E. (2009). Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not. Animal Reproduction Science, 112(1–2), 22–35. https://doi.org/10.1016/J.ANIREPROSCI.2008.03.016
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Reconocimiento 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconocimiento 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv xx, 136 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.country.none.fl_str_mv Colombia
dc.publisher.spa.fl_str_mv Universidad Nocional de Colombia
dc.publisher.program.spa.fl_str_mv Medellín - Ciencias - Doctorado en Biotecnología
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Medellín
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/83352/9/1020417714.2022.pdf
https://repositorio.unal.edu.co/bitstream/unal/83352/8/license.txt
https://repositorio.unal.edu.co/bitstream/unal/83352/10/1020417714.2022%20Licencia%20Cap%c3%adtulo%202.pdf
https://repositorio.unal.edu.co/bitstream/unal/83352/11/1020417714.2022%20Licencia%20Cap%c3%adtulo%204.pdf
https://repositorio.unal.edu.co/bitstream/unal/83352/12/1020417714.2022.pdf.jpg
bitstream.checksum.fl_str_mv a315a4b21e1a63aa3d486093873db023
eb34b1cf90b7e1103fc9dfd26be24b4a
c1e98cf5e4444dffc73f27aade9db2b6
dd4452136b217c4fdbf948ec4dbf11d4
cb8df17dc3daced9089ff2408b245b38
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1814089873515085824
spelling Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Restrepo Betancur, Giovannibd03434c72051e913ab3e03d3c37d88d600Usuga Suarez, Alexandrace1370743e5b92c3d7204110830e1903Montoya Páez, Juan David3be313d6aba0843a37f2fcf37be22b71600Úsuga Suárez, AlexandraRestrepo Betancur, GiovanniRojano, Benjamín AlbertoRestrepo Betancur, GiovanniGrupo de Investigación en Biotecnología Animal GIBAQuímica de los Productos Naturales y los AlimentosMontoya Páez, Juan David [0000-0002-9677-0426]Montoya Páez, Juan DavidMontoya Páez, Juan David2023-02-07T16:01:10Z2023-02-07T16:01:10Z2022-11-04https://repositorio.unal.edu.co/handle/unal/83352Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasActualmente las razas de asnos se encuentran en grave peligro de extinción, donde se ha producido una disminución sustancial en el tamaño de la población. En Colombia se reportó que la población de asnos criollos en el país para el 2010 era de 134251 animales y para el año 2015 hubo una dramática reducción a 75072 ejemplares. Una de las consecuencias de la reducción de la población en los asnos, se ha dado por la exportación indiscriminada de pieles de asno hacia la China, para la producción de un medicamento tradicional, lo que ha conllevado a nivel mundial a una reducción considerable en la población. Dado esto, la criopreservación es una técnica que permite mantener las células a baja temperatura sin perder su viabilidad y utilidad, tiene como objetivo inhibir la actividad metabólica del semen, garantizando su vitalidad y función a través del tiempo, conservándolo indefinidamente. Sin embargo, las tasas de fertilidad con semen criopreservado en programas de inseminación artificial siguen siendo menores con respecto al semen fresco o refrigerado, esto es dado a que los procesos de congelación en el semen incluyen factores que son extremadamente nocivos para las células, que afectan seriamente la vitalidad y funcionalidad de los espermatozoides reduciendo su longevidad. Específicamente el uso de la inseminación artificial en las burras ha tenido algunas dificultades en comparación a las yeguas, debido a las diferencias anatómicas y fisiológicas en el tracto reproductivo. Después de la inseminación artificial con semen descongelado se provoca una reacción inflamatoria en el útero probablemente generado por la ausencia del plasma seminal y los crioprotectores permeables en el semen. El objetivo de esta investigación fue evaluar factores asociados con la criotoleracia, el estado redox y la capacidad fecundante del semen asnal, orientados a la conservación de los asnos (Equus asinus) criollos colombianos. Para lo anterior se propuso la evaluación de 8 alternativas en la congelación del semen con diferentes combinaciones entre crioprotectores permeables y no permeables como la dimetilformamida (DMF), sacarosa (SAC), albumina sérica bovina (BSA) y plasma seminal (PS). Para esto fueron colectados 30 eyaculados, de cada eyaculado se extrajo una proporción de plasma seminal sobre el cual fueron analizadas proteínas y enzimas. Al semen, sometido a las diferentes alternativas de criopreservación, se les realizó evaluación de la calidad seminal, capacidad antioxidante total (TAC), especies reactivas de oxigeno (ERO) y capacidad fecundante. Para el análisis estadístico se ajustaron modelos lineales generalizados (GLM) y se realizaron correlaciones y regresiones. Se encontraron asociaciones entre el contenido del plasma, la TAC y EROS sobre la calidad del semen fresco y descongelado de asnos. Así mismo, se encontró que la combinación entre DMF, BSA y PS brinda una mayor adherencia de espermatozoides a la zona pelúcida. La selección de los componentes de los diluyentes en la congelación de semen de asnos criollos colombianos, es determinante en la conservación, en el aporte antioxidante, en la mitigación de especies reactivas de oxígeno y en la capacidad fecundante. (Texto tomado de la fuente)Donkey breeds are currently in serious danger of extinction, where there has been a substantial decline in population size. In Colombia, it was reported that the population of Creole donkeys for 2010 was 134,251 animals and for the year 2015 there was a dramatic reduction to 75,072 specimens. One of the consequences of the reduction in the population of donkeys has been the indiscriminate export of donkey skins to China for the production of a traditional medicine, which has led to a considerable reduction in the population. For this reason, cryopreservation is a technique that allows cells to be kept at a low temperature without lose their viability and usefulness. Its objective is to inhibit the metabolic activity of sperm, guaranteeing its vitality and function over time, preserving it indefinitely. However, fertility rates with cryopreserved semen in artificial insemination programs continue to be lower compared to fresh or chilled semen, this is because the freezing processes in semen include factors that are extremely harmful to cells that seriously affect fertility, vitality and functionality of spermatozoa, reducing their longevity. Specifically, the use of artificial insemination in donkeys has had some difficulties compared to mares due to anatomical and physiological differences in the reproductive tract. After artificial insemination with thawed semen, it causes an inflammatory reaction in the uterus, probably caused by the absence of seminal plasma and permeable cryoprotectants in the semen. The aim of this research was to evaluate factors associated with the cryotolerance, redox status and fertilizing capacity of donkey semen, aimed at the conservation of Colombian Creole donkeys (Equus asinus). For this, the evaluation of 8 alternatives in semen freezing was carried out with different combinations between permeable and non-permeable cryoprotectants such as dimethylformamide (DMF), sucrose (SAC), bovine serum albumin (BSA) and seminal plasma (PS). Thirty (30) ejaculates were collected, from each ejaculate Contenido XIX a proportion of seminal plasma was extracted, from which proteins and enzymes were analyzed. The semen with different cryopreservation alternatives, was evaluated for seminal quality, total antioxidant capacity (TAC), reactive oxygen species (ROS) and fertilizing capacity. For statistical analysis, generalized linear models (GLM) were fitted and correlations and regressions were performed. Associations were found between plasma content, TAC and EROS on the quality of fresh and thawed donkey semen. In addition, it was found that the combination of DMF, BSA and PS provides greater adherence of sperm to the zona pellucida. The selection of the components of the extenders in the freezing of semen of Colombian Creole donkeys, is decisive in its conservation, in the antioxidant contribution, in the mitigation of reactive oxygen species and in its fertilizing capacity.DoctoradoDoctor en BiotecnologíaBiotecnología de la Reproducción AnimalÁrea curricular Biotecnologíaxx, 136 páginasapplication/pdfspaUniversidad Nocional de ColombiaMedellín - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - Biología590 - Animales::599 - MamíferosCriopreservacion de órganos, tejidos. etc.Conservación del semenSemen - CriopreservaciónCryopreservation of organs, tissues, etc.Semen conservationBurroAsnoSemenPlasma seminalCrioprotector permeableCrioprotector no permeableCalidad seminalCapacidad antioxidanteEspecies reactivas de oxígenoSeminal plasmaPermeable cryoprotectantNon-permeable cryoprotectantSeminal qualityAntioxidant capacityReactive oxygen speciesEvaluación de factores asociados con la criotolerancia, el estado rédox y la capacidad fecundante del semen asnal, orientados a la conservación de los burros (Equus asinus) criollos colombianosEvaluation of factors associated with cryotolerance, redox state and fertilizing capacity of asinine semen, aimed at the conservation of colombian creole donkeys (Equus asinus)Trabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDColombiaRedColLaReferenciaAebi, H. (1984). Catalase in vitro. In Antioxidant Enzymes of Seminal Plasma International Braz J Urol (Vol. 34, Issue 4, pp. 121–126). https://doi.org/10.1016/S0076-6879(84)05016-3Agarwal, A., Gupta, S., & Sikka, S. (2006). The role of free radicals and antioxidants in reproduction. Current Opinion in Obstetrics & Gynecology, 18(3), 325–332. https://doi.org/10.1097/01.GCO.0000193003.58158.4EAitken, J., & Fisher, H. (1994). Reactive oxygen species generation and human spermatozoa: The balance of benefit and risk. BioEssays, 16(4), 259–267. https://doi.org/10.1002/BIES.950160409Arts, M. J. T. ., Sebastiaan Dallinga, J., Voss, H.-P., Haenen, G. R. M. ., & Bast, A. (2004). A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chemistry, 88(4), 567–570. https://doi.org/10.1016/j.foodchem.2004.02.008Balao da Silva, C. M., Spinaci, M., Bucci, D., Giaretta, E., Peña, F. J., Mari, G., & Galeati, G. (2013). Effect of sex sorting on stallion spermatozoa: Heterologous oocyte binding, tyrosine phosphorylation and acrosome reaction assay. Animal Reproduction Science, 141(1–2), 68–74. https://doi.org/10.1016/J.ANIREPROSCI.2013.07.008Ball, B. A. (2008). Oxidative stress, osmotic stress and apoptosis: Impacts on sperm function and preservation in the horse. Animal Reproduction Science, 107(3–4), 257–267. https://doi.org/10.1016/J.ANIREPROSCI.2008.04.014Ball, B. A., Vo, A. T., & Baumber, J. (2001). Generation of reactive oxygen species by equine spermatozoa. American Journal of Veterinary Research, 62(4), 508–515. https://doi.org/10.2460/AJVR.2001.62.508Barth, A. D., & Oko, R. J. (1989). Abnormal morphology of bovine spermatozoa (1st ed.). Iowa State University Press.Benítez-Estrada, A., Villanueva-Sánchez, J., González-Rosendo, G., Alcántar-Rodríguez, V. E., Puga-Díaz, R., Quintero-Gutiérrez, A. G., Benítez-Estrada, A., Villanueva-Sánchez, J., González-Rosendo, G., Alcántar-Rodríguez, V. E., Puga-Díaz, R., & Quintero-Gutiérrez, A. G. (2020). Determinación de la capacidad antioxidante total de alimentos y plasma humano por fotoquimioluminiscencia: Correlación con ensayos fluorométricos (ORAC) y espectrofotométricos (FRAP). TIP. Revista Especializada En Ciencias Químico-Biológicas, 23, 1–9. https://doi.org/10.22201/FESZ.23958723E.2020.0.244Bhagat, S., Soni, A., Mishra, S., Santra, A., Sonkar, N., & Verma, U. (2020). Body condition scoring of Equine: Short note. ~ 19 ~ The Pharma Innovation Journal, 9(3), 19–20. http://www.thepharmajournal.comBradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3Brahmkshtri, B. P., Edwin, M. J., John, M. C., Nainar, A. M., & Krishnan, A. R. (1999). Relative efficacy of conventional sperm parameters and sperm penetration bioassay to assess bull fertility in vitro. Animal Reproduction Science, 54(3), 159–168. https://doi.org/10.1016/S0378-4320(98)00108-0Bresnahan, D. R., Lupole, R. E., Stilz, C. R., & Carnevale, E. M. (2022). Effect of Mare Age on Transcript Abundance of Connexins-37 and -43, Zona Pellucida Proteins, and Sperm Binding. Journal of Equine Veterinary Science, 108, 103796. https://doi.org/10.1016/J.JEVS.2021.103796Brito, L. F. C., Greene, L. M., Kelleman, A., Knobbe, M., & Turner, R. (2011). Effect of method and clinician on stallion sperm morphology evaluation. Theriogenology. https://doi.org/10.1016/j.theriogenology.2011.04.007Bucci, D., Giaretta, E., Spinaci, M., Rizzato, G., Isani, G., Mislei, B., Mari, G., Tamanini, C., & Galeati, G. (2016). Characterization of alkaline phosphatase activity in seminal plasma and in fresh and frozen–thawed stallion spermatozoa. Theriogenology, 85(2), 288-295.e2. https://doi.org/10.1016/J.THERIOGENOLOGY.2015.09.007Carver, D. A., & Ball, B. A. (2002). Lipase activity in stallion seminal plasma and the effect of lipase on stallion spermatozoa during storage at 5 °C. Theriogenology, 58(8), 1587–1595. https://doi.org/10.1016/S0093-691X(02)01049-XCatalán, J., Yánez-Ortiz, I., Tvarijonaviciute, A., González-Arostegui, L. G., Rubio, C. P., Yeste, M., Miró, J., & Barranco, I. (2022). Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance. Antioxidants, 11(2), 417. https://doi.org/10.3390/ANTIOX11020417/S1Cecchini Gualandi, S., Giangaspero, B., Di Palma, T., Macchia, G., Carluccio, A., & Boni, R. (2021). Oxidative profile and protease regulator potential to predict sperm functionality in donkey (Equus asinus). Scientific Reports 2021 11:1, 11(1), 1–10. https://doi.org/10.1038/s41598-021-99972-9Cohen, D. J., Maldera, J. A., Weigel Muñoz, M., Ernesto, J. I., Vasen, G., & Cuasnicu, P. S. (2011). Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization. Biological Research, 44(2), 135–138. https://doi.org/10.4067/S0716-97602011000200004Consuegra, C., Crespo, F., Dorado, J., Diaz-Jimenez, M., Pereira, B., Sánchez-Calabuig, M. J., Beltrán-Breña, P., Pérez-Cerezales, S., Rizos, D., & Hidalgo, M. (2020). Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures. Animal Reproduction Science, 223, 106627. https://doi.org/10.1016/J.ANIREPROSCI.2020.106627Contri, A., Gloria, A., Robbe, D., Sfirro, M. P., & Carluccio, A. (2012). Effect of sperm concentration on characteristics of frozen-thawed semen in donkeys. Animal Reproduction Science, 136(1–2), 74–80. https://doi.org/10.1016/J.ANIREPROSCI.2012.10.022Dacheux, J. L., Castella, S., Gatti, J. L., & Dacheux, F. (2005). Epididymal cell secretory activities and the role of proteins in boar sperm maturation. Theriogenology, 63(2), 319–341. https://doi.org/10.1016/J.THERIOGENOLOGY.2004.09.015Dominguez, E. M., Moreno-Irusta, A., Rodriguez, M. B., Salamone, D. F., de Arruda, R. P., Losinno, L., & Giojalas, L. C. (2020). Chemotactic selection of frozen-thawed stallion sperm improves sperm quality and heterologous binding to oocytes. Animal Reproduction Science, 221, 106582. https://doi.org/10.1016/J.ANIREPROSCI.2020.106582Gamboa, S., Rodrigues, A. S., Henriques, L., Batista, C., & Ramalho-Santos, J. (2010). Seasonal functional relevance of sperm characteristics in equine spermatozoa. Theriogenology, 73(7), 950–958. https://doi.org/10.1016/J.THERIOGENOLOGY.2009.11.023Giesecke, K., Sieme, H., & Distl, O. (2010). Infertility and candidate gene markers for fertility in stallions: A review. The Veterinary Journal, 185(3), 265–271. https://doi.org/10.1016/J.TVJL.2009.07.024Graham, J. K., & Mocé, E. (2005). Fertility evaluation of frozen/thawed semen. Theriogenology, 64(3), 492–504. https://doi.org/10.1016/J.THERIOGENOLOGY.2005.05.006Guthrie, H. D., & Welch, G. R. (2006). Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry1. Journal of Animal Science, 84(8), 2089–2100. https://doi.org/10.2527/jas.2005-766Hamann, H., Jude, R., Sieme, H., Mertens, U., Töpfer-Petersen, E., Distl, O., & Leeb, T. (2007). A polymorphism within the equine CRISP3 gene is associated with stallion fertility in Hanoverian warmblood horses. Animal Genetics, 38(3), 259–264. https://doi.org/10.1111/J.1365-2052.2007.01594.XHärtlová, H., Rajmon, R., Krontorádová, I., Mamica, J., Zita, L., Klabanová, P., & Černocký, A. (2013). Semen quality, lipid peroxidation, and seminal plasma antioxidant status in horses with different intensities of physical exercise. Acta Veterinaria Brno, 82(1), 31–35. https://doi.org/10.2754/avb201382010031Hasegawa, A., & Koyama, K. (2007). Contribution of zona proteins to oocyte growth. Society of Reproduction and Fertility Supplement, 63, 229–235. https://pubmed.ncbi.nlm.nih.gov/17566276/Kankofer, M., Kolm, G., Aurich, J., & Aurich, C. (2005). Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5°C. Theriogenology, 63(5), 1354–1365. https://doi.org/10.1016/J.THERIOGENOLOGY.2004.07.005Kareskoski, M., & Katila, T. (2008). Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity. Animal Reproduction Science, 107(3–4), 249–256. https://doi.org/10.1016/J.ANIREPROSCI.2008.04.013Len, J., Beehan, D., Eilts, B., Ebrahimie, E., & Lyle, S. (2020). Stallion Sperm Integrity After Centrifugation to Reduce Seminal Plasma Concentration and Cool Storage for 4 days. Journal of Equine Veterinary Science, 85. https://doi.org/10.1016/J.JEVS.2019.102819Maeda, T., Nishida, J., & Nakanishi, Y. (1999). Expression pattern, subcellular localization and structure--function relationship of rat Tpx-1, a spermatogenic cell adhesion molecule responsible for association with Sertoli cells. Development, Growth & Differentiation, 41(6), 715–722. https://doi.org/10.1046/J.1440-169X.1999.00470.XMagdaleno, L., Gasset, M., Varea, J., Schambony, A. M., Urbanke, C., Raida, M., Töpfer-Petersen, E., & Calvete, J. J. (1997). Biochemical and conformational characterisation of HSP-3, a stallion seminal plasma protein of the cysteine-rich secretory protein (CRISP) family. FEBS Letters, 420(2–3), 179–185. https://doi.org/10.1016/S0014-5793(97)01514-7Marklund, S., & Marklund, G. (1974). Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase. European Journal of Biochemistry, 47(3), 469–474. https://doi.org/10.1111/J.1432-1033.1974.TB03714.XMichael, A., Alexopoulos, C., Pontiki, E., Hadjipavlou-Litina, D., Saratsis, P., & Boscos, C. (2007). Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology, 68(2), 204–212. https://doi.org/10.1016/J.THERIOGENOLOGY.2007.04.053Miragaya, M. H., Neild, D. M., & Alonso, A. E. (2018). A Review of Reproductive Biology and Biotechnologies in Donkeys. Journal of Equine Veterinary Science, 65, 55–61. https://doi.org/10.1016/J.JEVS.2017.12.005Miró, J, Taberner, E., Rivera, M., Peña, A., Medrano, A., Rigau, T., & Peñalba, A. (2009). Effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey semen. Theriogenology, 72(8), 1017–1022. https://doi.org/10.1016/j.theriogenology.2009.06.012Miró, Jordi, Vilés, K., García, W., Jordana, J., & Yeste, M. (2013). Effect of donkey seminal plasma on sperm movement and sperm-polymorphonuclear neutrophils attachment in vitro. Animal Reproduction Science, 140, 164–172. https://doi.org/10.1016/j.anireprosci.2013.06.007Monteiro, G. A., Papa, F. O., Zahn, F. S., Dellaqua, J. A., Melo, C. M., Maziero, R. R. D., Avanzi, B. R., Alvarenga, M. A., & Guasti, P. N. (2011). Cryopreservation and fertility of ejaculated and epididymal stallion sperm. Animal Reproduction Science, 127(3–4), 197–201. https://doi.org/10.1016/j.anireprosci.2011.08.002Monteiro, R. A., Cunha, R. M., Guerra, M. M. P., de Almeida, V. M., Peña-Alfaro, C. E., & Silva, S. V. (2022). Use of Equine Sperm Cryopreservation Techniques as a Conservation Method of Donkey Germplasm. Journal of Equine Veterinary Science, 111, 103888. https://doi.org/10.1016/j.jevs.2022.103888Montoya, J. D., Rojano, B., & Restrepo, G. (2017). Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación. Archivos de Zootecnia, 66(255), 333. https://doi.org/10.21071/az.v66i255.2508Montoya Páez, J. D., Rojano, B. A., Restrepo Betancur, G., Montoya Páez, J. D., Rojano, B. A., & Restrepo Betancur, G. (2017). Suplementación con plasma seminal y relación de sus componentes con la calidad de semen congelado-descongelado de asnos (Equus asinus). Revista Mexicana de Ciencias Pecuarias, 8(3), 233–242. https://doi.org/10.22319/RMCP.V8I3.4499Muñoz, M. W., Ernesto, J. I., Bluguermann, C., Busso, D., Battistone, M. A., Cohen, D. J., & Cuasnicú, P. S. (2012). Evaluation of testicular sperm CRISP2 as a potential target for contraception. Journal of Andrology, 33(6), 1360–1370. https://doi.org/10.2164/JANDROL.112.016725Neild, D., Chaves, G., Flores, M., Mora, N., Beconi, M., & Agüero, A. (1999). Hypoosmotic test in equine spermatozoa. Theriogenology. https://doi.org/10.1016/S0093-691X(99)00021-7Neuhauser, S., Gösele, P., & Handler, J. (2019). Postthaw Addition of Autologous Seminal Plasma Improves Sperm Motion Characteristics in Fair and Poor Freezer Stallions. Journal of Equine Veterinary Science, 72, 117–123. https://doi.org/10.1016/J.JEVS.2018.10.028Nouri, H., Towhidi, A., Zhandi, M., & Sadeghi, R. (2013). The effects of centrifuged egg yolk used with INRA plus soybean lecithin extender on semen quality to freeze miniature caspian horse semen. Journal of Equine Veterinary Science, 33(12), 1050–1053. https://doi.org/10.1016/J.JEVS.2013.03.184Ortega-Ferrusola, C., Johannisson, A., Peña Vega, F. J., Tapia, J. A., Rodriguez-Martinez, H., Dalin, A. M., & Morrell, J. M. (2011). Effect of Different Extenders and Seminal Plasma on the Susceptibility of Equine Spermatozoa to Lipid Peroxidation After Single-Layer Centrifugation, Through Androcoll-E. Journal of Equine Veterinary Science, 31(7), 411–416. https://doi.org/10.1016/J.JEVS.2011.01.010Ortiz, I., Dorado, J., Acha, D., Gálvez, M. J., Urbano, M., & Hidalgo, M. (2015). Colloid single-layer centrifugation improves post-thaw donkey (Equus asinus) sperm quality and is related to ejaculate freezability. Reproduction, Fertility and Development, 27(2), 332. https://doi.org/10.1071/RD13246Ou, B., Hampsch-Woodill, M., & Prior, R. L. (2001). Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe. Journal of Agricultural and Food Chemistry, 49(10), 4619–4626. https://doi.org/10.1021/jf010586oPapa, F. O., Melo, C. M., Fioratti, E. G., Dell’Aqua, J. A., Zahn, F. S., & Alvarenga, M. A. (2008). Freezing of stallion epididymal sperm. Animal Reproduction Science, 107(3–4), 293–301. https://doi.org/10.1016/J.ANIREPROSCI.2008.05.003Papas, M., Catalan, J., Barranco, I., Arroyo, L., Bassols, A., Yeste, M., & Miró, J. (2020). Total and specific activities of superoxide dismutase (SOD) in seminal plasma are related with the cryotolerance of jackass spermatozoa. Cryobiology, 92, 109–116. https://doi.org/10.1016/J.CRYOBIOL.2019.11.043Pontiki, E., & Hadjipavlou-Litina, D. (2006). Antioxidant and Anti-Inflammatory Activity of Aryl-Acetic and Hydroxamic Acids as Novel Lipoxygenase Inhibitors. Medicinal Chemistry, 2(3), 251–264. https://doi.org/10.2174/157340606776930763Portus, B. J., Reilas, T., & Katila, T. (2005). Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares. Equine Veterinary Journal, 37(6), 515–519. https://doi.org/10.2746/042516405775314844Rodríguez-Martínez, H., Kvist, U., Ernerudh, J., Sanz, L., & Calvete, J. J. (2011). Seminal Plasma Proteins: What Role Do They Play? American Journal of Reproductive Immunology, 66(SUPPL. 1), 11–22. https://doi.org/10.1111/j.1600-0897.2011.01033.xSchambony, A., Gentzel, M., Wolfes, H., Raida, M., Neumann, U., & Töpfer-Petersen, E. (1998). Equine CRISP-3: primary structure and expression in the male genital tract. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1387(1–2), 206–216. https://doi.org/10.1016/S0167-4838(98)00122-8Serres, C., Rodriguez, A., Alvarez, A. L., Santiago, I., Gabriel, J., Gómez-Cuétara, C., & Mateos, E. (2002). Effect of centrifugation and temperature on the motility and plasma membrane integrity of Zamorano-Leonés donkey semen. Theriogenology, 58(2–4), 329–332. https://doi.org/10.1016/S0093-691X(02)00876-2Shamsi, M. B., Venkatesh, S., Tanwar, M., Talwar, P., Sharma, R. K., Dhawan, A., Kumar, R., Gupta, N. P., Malhotra, N., Singh, N., Mittal, S., & Dada, R. (2009). DNA integrity and semen quality in men with low seminal antioxidant levels. Mutation Research, 665(1–2), 29–36. https://doi.org/10.1016/J.MRFMMM.2009.02.017Töpfer-Petersen, E., Ekhlasi-Hundrieser, M., Kirchhoff, C., Leeb, T., & Sieme, H. (2005). The role of stallion seminal proteins in fertilisation. Animal Reproduction Science, 89(1–4), 159–170. https://doi.org/10.1016/J.ANIREPROSCI.2005.06.018Troedsson, M. H., Lee, C. S., Franklin, R. D., & Crabo, B. G. (2000). The role of seminal plasma in post-breeding uterine inflammation. Journal of Reproduction and Fertility. Supplement, 56, 341–349. https://europepmc.org/article/med/20681146Usuga, A., Rojano, B. A., Restrepo, G., Usuga, A., Rojano, B. A., & Restrepo, G. (2018). Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen. Reproduction, Fertility and Development, 30(3), 563–569. https://doi.org/10.1071/RD17044Usuga, A., Rojano, B., & Restrepo, G. (2020). Lyophilized seminal plasma can improve stallion semen freezability. Indian Journal of Animal Sciences, 90(2), 171–175. https://doi.org/10.13140/RG.2.2.25756.03206Vilés, K., Rabanal, R., Rodríguez-Prado, M., & Miró, J. (2013). Effect of ketoprofen treatment on the uterine inflammatory response after AI of jennies with frozen semen. Theriogenology, 79, 1019–1026. https://doi.org/10.1016/j.theriogenology.2013.01.006Waheed, M. M., El-Bahr, S. M., & Al-haider, A. K. (2013). Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse. Journal of Equine Veterinary Science, 33(9), 705–709. https://doi.org/10.1016/J.JEVS.2012.11.006Wittayarat, M., Pukazhenthi, B. S., Tipkantha, W., Techakumphu, M., Srisuwatanasagul, S., & Panyaboriban, S. (2021). CRISP protein expression in semen of the endangered Malayan tapir (Tapirus indicus). Theriogenology, 172, 106–115. https://doi.org/10.1016/J.THERIOGENOLOGY.2021.06.005Wood, P. L., Scoggin, K., Ball, B. A., Troedsson, M. H., & Squires, E. L. (2016). Lipidomics of equine sperm and seminal plasma: Identification of amphiphilic (O-acyl)-ω-hydroxy-fatty acids. Theriogenology, 86(5), 1212–1221. https://doi.org/10.1016/J.THERIOGENOLOGY.2016.04.012Yoshida, M. (2000). Conservation of sperms: Current status and new trends. Animal Reproduction Science, 60–61, 349–355. https://doi.org/10.1016/S0378-4320(00)00125-1Bottrel, M., Acha, D., Ortiz, I., Hidalgo, M., Gósalvez, J., Camisão, J., & Dorado, J. (2018). Cryoprotective effect of glutamine, taurine, and proline on post-thaw semen quality and DNA integrity of donkey spermatozoa. Animal Reproduction Science, 189, 128–135. https://doi.org/10.1016/J.ANIREPROSCI.2017.12.021Burden, F., & Thiemann, A. (2015). Donkeys Are Different. Journal of Equine Veterinary Science, 35(5), 376–382. https://doi.org/10.1016/J.JEVS.2015.03.005Camillo, F., Rota, A., Biagini, L., Tesi, M., Fanelli, D., & Panzani, D. (2018). The Current Situation and Trend of Donkey Industry in Europe. Journal of Equine Veterinary Science, 65, 44–49. https://doi.org/10.1016/J.JEVS.2017.11.008Contri, A., Gloria, A., Robbe, D., Sfirro, M. P., & Carluccio, A. (2012). Effect of sperm concentration on characteristics of frozen-thawed semen in donkeys. Animal Reproduction Science, 136(1–2), 74–80. https://doi.org/10.1016/J.ANIREPROSCI.2012.10.022Food and Agricultural Organization (FAO). Commission on genetic resources for food and agriculture: Status and trends of Animal Genetic Resources. Disponible en: http://www.fao.org/3/a-mm278e.pdf. 2018Guasti, P. N., Monteiro, G. A., & Papa, F. O. (2012). Componentes do plasma seminal e sua influência sobre a criopreservação e fertilidade de espermatozóides equinos. Veterinária e Zootecnia. https://repositorio.unesp.br/handle/11449/141249?show=fullKareskoski, M., & Katila, T. (2008). Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity. Animal Reproduction Science, 107(3–4), 249–256. https://doi.org/10.1016/J.ANIREPROSCI.2008.04.013Leeb, T., Sieme, H., & Töpfer-Petersen, E. (2005). Genetic markers for stallion fertility--lessons from humans and mice. Animal Reproduction Science, 89(1–4), 21–29. https://doi.org/10.1016/J.ANIREPROSCI.2005.06.021Macías García, B., Ortega Ferrusola, C., Aparicio, I. M., Miró-Morán, A., Morillo Rodriguez, A., Gallardo Bolaños, J. M., González Fernández, L., Balao da Silva, C. M., Rodríguez Martínez, H., Tapia, J. A., & Peña, F. J. (2012). Toxicity of glycerol for the stallion spermatozoa: effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential. Theriogenology, 77(7), 1280–1289. https://doi.org/10.1016/J.THERIOGENOLOGY.2011.10.033Madison, R. J., Evans, L. E., & Youngs, C. R. (2013). The Effect of 2-Hydroxypropyl-β-Cyclodextrin on Post-Thaw Parameters of Cryopreserved Jack and Stallion Semen. Journal of Equine Veterinary Science, 33(4), 272–278. https://doi.org/10.1016/j.jevs.2012.07.021Ministerio De Agricultura Y Desarrollo Rural De Colombia. 2020. Cadena equina, asnal y mular. Documento disponible en: https://sioc.minagricultura.gov.co/Equino/Documentos/005%20-%20Documentos%20T%C3%A9cnicos/Diagnostico%20Cadena%20Equina,%20Asnal%20y%20Mular.pdf. Consultado el 10 de abril de 2022Miró, Jordi, Viles, K., Fernandez, M., Fabregas, N., Soares, J., & Garcia, W. (2011). Induced acute endometritis by frozen semen insemination in donkey | Request PDF. Reproduction in Domestic Animals. https://www.researchgate.net/publication/313077361_Induced_acute_endometritis_by_frozen_semen_insemination_in_donkeyMonteiro, R. A., Cunha, R. M., Guerra, M. M. P., de Almeida, V. M., Peña-Alfaro, C. E., & Silva, S. V. (2022). Use of Equine Sperm Cryopreservation Techniques as a Conservation Method of Donkey Germplasm. Journal of Equine Veterinary Science, 111, 103888. https://doi.org/10.1016/j.jevs.2022.103888Oliveira, J. V. de, Oliveira, P. V. de L. F., Melo e Oña, C. M., Guasti, P. N., Monteiro, G. A., Sancler da Silva, Y. F. R., Papa, P. de M., Alvarenga, M. A., Dell’Aqua Junior, J. A., & Papa, F. O. (2016). Strategies to improve the fertility of fresh and frozen donkey semen. Theriogenology, 85(7), 1267–1273. https://doi.org/10.1016/j.theriogenology.2015.12.010Panzani, D., Rota, A., Tesi, M., Fanelli, D., & Camillo, F. (2018). Update on Donkey Embryo Transfer and Cryopreservation. Journal of Equine Veterinary Science, 65, 50–54. https://doi.org/10.1016/J.JEVS.2017.11.012Papa, F. O., Melo, C. M., Fioratti, E. G., Dell’Aqua, J. A., Zahn, F. S., & Alvarenga, M. A. (2008). Freezing of stallion epididymal sperm. Animal Reproduction Science, 107(3–4), 293–301. https://doi.org/10.1016/J.ANIREPROSCI.2008.05.003Revista Semana. El trágico destino de los burros que se desaparecen en la Costa. Tomado de https://www.semana.com/nacion/articulo/desaparecen-burros-en-lacosta/519103. 2017Rodriguez-Martinez, H., & Wallgren, M. (2011). Advances in Boar Semen Cryopreservation. Veterinary Medicine International, 2011. https://doi.org/10.4061/2011/396181Rossel, S., Marshall, F., Peters, J., Pilgram, T., Adams, M. D., & O’Connor, D. (2008). Domestication of the donkey: Timing, processes, and indicators. Proceedings of the National Academy of Sciences of the United States of America, 105(10), 3715–3720. https://doi.org/10.1073/PNAS.0709692105/SUPPL_FILE/INDEX.HTMLRota, A., Panzani, D., Sabatini, C., & Camillo, F. (2012). Donkey jack (Equus asinus) semen cryopreservation: Studies of seminal parameters, post breeding inflammatory response, and fertility in donkey jennies. Theriogenology, 78(8), 1846–1854. https://doi.org/10.1016/J.THERIOGENOLOGY.2012.07.015Rota, Alessandra, Puddu, B., Sabatini, C., Panzani, D., Lainé, A. L., & Camillo, F. (2018). Reproductive parameters of donkey jacks undergoing puberty. Animal Reproduction Science, 192, 119–125. https://doi.org/10.1016/J.ANIREPROSCI.2018.02.02Serres, C., Rodriguez, A., Alvarez, A. L., Santiago, I., Gabriel, J., Gómez-Cuétara, C., & Mateos, E. (2002). Effect of centrifugation and temperature on the motility and plasma membrane integrity of Zamorano-Leonés donkey semen. Theriogenology, 58(2–4), 329–332. https://doi.org/10.1016/S0093-691X(02)00876-2The Donkey Sanctuary. What is ejiao? Tomado de www.thedonkeysanctuary.org.uk. 2017Vidament, M., Vincent, P., Martin, F. X., Magistrini, M., & Blesbois, E. (2009). Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not. Animal Reproduction Science, 112(1–2), 22–35. https://doi.org/10.1016/J.ANIREPROSCI.2008.03.016Politécnico Colombiano Jaime Isaza CadavidUniversidad Nacional de ColombiaColcienciasEstudiantesInvestigadoresMaestrosORIGINAL1020417714.2022.pdf1020417714.2022.pdfTesis de Doctorado en Biotecnologíaapplication/pdf1334230https://repositorio.unal.edu.co/bitstream/unal/83352/9/1020417714.2022.pdfa315a4b21e1a63aa3d486093873db023MD59LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83352/8/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD58CC-LICENSE1020417714.2022 Licencia Capítulo 2.pdf1020417714.2022 Licencia Capítulo 2.pdfLicencia capitulo 2application/pdf281249https://repositorio.unal.edu.co/bitstream/unal/83352/10/1020417714.2022%20Licencia%20Cap%c3%adtulo%202.pdfc1e98cf5e4444dffc73f27aade9db2b6MD5101020417714.2022 Licencia Capítulo 4.pdf1020417714.2022 Licencia Capítulo 4.pdfLicencia capitulo 4application/pdf294860https://repositorio.unal.edu.co/bitstream/unal/83352/11/1020417714.2022%20Licencia%20Cap%c3%adtulo%204.pdfdd4452136b217c4fdbf948ec4dbf11d4MD511THUMBNAIL1020417714.2022.pdf.jpg1020417714.2022.pdf.jpgGenerated Thumbnailimage/jpeg6073https://repositorio.unal.edu.co/bitstream/unal/83352/12/1020417714.2022.pdf.jpgcb8df17dc3daced9089ff2408b245b38MD512unal/83352oai:repositorio.unal.edu.co:unal/833522024-08-15 23:14:50.421Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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

Publicaciones similares