Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos

ilustraciones, diagramas, fotografías

Autores:: Tamayo Figueroa, Diana Paola

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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repository_id_str
dc.title.spa.fl_str_mv	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
dc.title.translated.eng.fl_str_mv	Repair of cracks in cement-based materials using axenic and mixed bacterial cultures
title	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
spellingShingle	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos 690 - Construcción de edificios::693 - Construcción en tipos específicos de materiales y propósitos específicos Ureasa Bacteria Precipitación de Calcita Inducida Microbiológicamente Materiales base cemento Grietas Urease Microbial induced calcite precipitation Cracks Cement based materials Materiales de construcción Biotecnología Microorganismo Building materials Biotechnology Microorganisms
title_short	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
title_full	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
title_fullStr	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
title_full_unstemmed	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
title_sort	Reparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtos
dc.creator.fl_str_mv	Tamayo Figueroa, Diana Paola
dc.contributor.advisor.spa.fl_str_mv	de Brito Brandão, Pedro Filipe Lizarazo Marriaga, Juan Manuel
dc.contributor.author.spa.fl_str_mv	Tamayo Figueroa, Diana Paola
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Estudios para la Remediación y Mitigación de Impactos Negativos al Ambiente Germina Análisis, Diseño y Materiales Gies
dc.contributor.cvlac.spa.fl_str_mv	https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000005690
dc.subject.ddc.spa.fl_str_mv	690 - Construcción de edificios::693 - Construcción en tipos específicos de materiales y propósitos específicos
topic	690 - Construcción de edificios::693 - Construcción en tipos específicos de materiales y propósitos específicos Ureasa Bacteria Precipitación de Calcita Inducida Microbiológicamente Materiales base cemento Grietas Urease Microbial induced calcite precipitation Cracks Cement based materials Materiales de construcción Biotecnología Microorganismo Building materials Biotechnology Microorganisms
dc.subject.proposal.spa.fl_str_mv	Ureasa Bacteria Precipitación de Calcita Inducida Microbiológicamente Materiales base cemento Grietas
dc.subject.proposal.eng.fl_str_mv	Urease Microbial induced calcite precipitation Cracks Cement based materials
dc.subject.unesco.spa.fl_str_mv	Materiales de construcción Biotecnología Microorganismo
dc.subject.unesco.eng.fl_str_mv	Building materials Biotechnology Microorganisms
description	ilustraciones, diagramas, fotografías
publishDate	2023
dc.date.issued.none.fl_str_mv	2023-11-21
dc.date.accessioned.none.fl_str_mv	2024-01-16T02:14:27Z
dc.date.available.none.fl_str_mv	2024-01-16T02:14:27Z
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/85319
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co
url	https://repositorio.unal.edu.co/handle/unal/85319 https://repositorio.unal.edu.co
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Doctorado en Biotecnología
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2de Brito Brandão, Pedro Filipe9ec73d77d555a4dff5e0457f24f26aefLizarazo Marriaga, Juan Manuel848bf0fb0ca1a5b59e5247b2bcf522d3600Tamayo Figueroa, Diana Paola8a6c9d7e65dfa274073ca50b081c249f600Grupo de Estudios para la Remediación y Mitigación de Impactos Negativos al Ambiente GerminaAnálisis, Diseño y Materiales Gieshttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=00000056902024-01-16T02:14:27Z2024-01-16T02:14:27Z2023-11-21https://repositorio.unal.edu.co/handle/unal/85319Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.coilustraciones, diagramas, fotografíasLa reparación de grietas en estructuras de construcción es un desafío común, donde la precipitación de calcita inducida microbiológicamente es una técnica prometedora. Allí, se emplean bacterias ureolíticas que alcalinizan el microambiente celular generando precipitación de calcita en las grietas, sellándolas. Esta investigación evaluó la reparación de grietas en materiales base cemento empleando cultivos bacterianos ureolíticos axénicos y mixtos mediante el aislamiento y caracterización de 49 microorganismos, donde se seleccionaron 4 correspondientes a los géneros Arthrobacter, Psychrobacillus, Glutamicibacter y Rhodococcus por su capacidad de precipitar el 99.7% (25 mM) de calcio en menos de 24 horas. Estas 4 bacterias fueron evaluadas en diferentes mezclas para establecer cultivos mixtos, donde el cultivo mixto con mayor actividad correspondió a la mezcla R. qingshengii S1 + A. crystallopoietes M4C20 + P. psycrodurans S17. Se determinó la estrategia de aplicación sobre el concreto, frecuencia y componentes del medio de cultivo, evidenciando que a mayor frecuencia de aplicación del microorganismo de manera directa sobre las grietas y empleando un biopolímero de dextrano (BILAC) se mejoró la eficiencia de la reparación acortando los tiempos iniciales en más del 50%. Finalmente, comparado con dos tratamientos comerciales, las probetas de mortero reparadas biotecnológicamente alcanzaron 5 veces más resistencia demostrando el potencial de aplicación de esta biotecnología en este campo. Para este estudio, el cultivo axénico de G. arilaitiensis M3C3 presentó mayor eficiencia que los cultivos mixtos, siendo este el primer reporte del uso de este microorganismo para la reparación de grietas en materiales base cemento empleando un biopolímero de dextrano. (Texto tomado de la fuente).Crack repair in building structures is a common challenge where microbiologically induced calcite precipitation (MICP) is a promising technique. In this process, ureolytic bacteria are used to alkalize the cellular microenvironment generating calcite precipitation in the cracks, sealing them. This study evaluated the crack reparation in cement-based materials using axenic and mixed ureolytic bacterial cultures by isolating and characterizing 49 strains, where 4 correspond to the genera Arthrobacter, Psychrobacillus, Glutamicibacter and Rhodococcus. These strains were selected for their ability to precipitate 99.7%. (25 mM) of calcium in less than 24 hours. These 4 bacteria were evaluated in different mixtures to establish mixed cultures, where the mixed culture with the highest activity corresponded to R. qingshengii S1 + A. crystallopoietes M4C20 + P. psycrodurans S17. The application strategy, frequency and components of the culture medium were determined, evidencing that the higher the frequency of application of the microorganism directly on the cracks and using a dextran biopolymer BILAC, improves the efficiency of the repair, shortening the initial times by more than 50%. Finally, compared with two commercial treatments, the biotechnologically repaired specimens reached 5 times more resistance, demonstrating the potential application of this biotechnology in this field. For this study, the axenic culture of G. arilaitiensis M3C3 presented greater efficiency than mixed cultures, being this the first report on the use of this microorganism for the repair of cracks in cement-based materials using a dextran biopolymer.DoctoradoDoctor en BiotecnologíaMicrobiología ambiental y aplicadaxxiii, 272 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá690 - Construcción de edificios::693 - Construcción en tipos específicos de materiales y propósitos específicosUreasaBacteriaPrecipitación de Calcita Inducida MicrobiológicamenteMateriales base cementoGrietasUreaseMicrobial induced calcite precipitationCracksCement based materialsMateriales de construcciónBiotecnologíaMicroorganismoBuilding materialsBiotechnologyMicroorganismsReparación de grietas en materiales base cemento empleando cultivos bacterianos axénicos y mixtosRepair of cracks in cement-based materials using axenic and mixed bacterial culturesTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAbadi, S., Azouri, D., Pupko, T., & Mayrose, I. 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In International Journal of Recent Technology and Engineering (Vol. 7, Issue 6C2, pp. 12–17). Blue Eyes Intelligence Engineering and Sciences PublicationSusilowati, P. E., Ardianti, D., Kartini, S., Sudiana, I. N., & Zaeni, A. (2021). Isolation of Ureolytic Bacteria of Soft Coral and Their Potential in Microbially Induced Calcite Precipitation (MICP). Journal of Physics: Conference Series, 1899(1), 012003. https://doi.org/10.1088/1742-6596/1899/1/012003Tziviloglou, E., Wiktor, V., Jonkers, H. M., & Schlangen, E. (2016). Bacteria-based self-healing concrete to increase liquid tightness of cracks. Construction and Building Materials, 122, 118–125. https://doi.org/10.1016/j.conbuildmat.2016.06.080Yoon, H. S., Lee, J. Y., Yang, K. H., & Park, S. H. (2022). Evaluation of the Crack Healing Efficiency of Mortar Incorporating Self-healing Pellets based on Cementitious Materials. Journal of the Architectural Institute of Korea, 38(4). https://doi.org/10.5659/JAIK.2022.38.4.207Precipitación de carbonatos inducida por microorganismos nativos de Colombia: su aprovechamiento y valoración en biomateriales y en la remediación de elementos tóxicosMinisterio de CienciasInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85319/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1018430894.2023.pdf1018430894.2023.pdfTesis de Doctorado en Biotecnologíaapplication/pdf9359501https://repositorio.unal.edu.co/bitstream/unal/85319/2/1018430894.2023.pdf390b37ec1fdaba0d379e42acde61fbe4MD52THUMBNAIL1018430894.2023.pdf.jpg1018430894.2023.pdf.jpgGenerated Thumbnailimage/jpeg5013https://repositorio.unal.edu.co/bitstream/unal/85319/3/1018430894.2023.pdf.jpge6640a9257678596fe1a082c8054954eMD53unal/85319oai:repositorio.unal.edu.co:unal/853192024-08-21 23:13:07.467Repositorio Institucional Universidad Nacional de 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