Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth

Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well a...

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Autores:
Medina Sierra, Marisol
Orozco P., Francisco Hernando
Márquez F., María Elena
Tipo de recurso:
Article of journal
Fecha de publicación:
2002
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/40942
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/40942
http://bdigital.unal.edu.co/31039/
Palabra clave:
kudzú (Pueraria phaseoloides)
zanahoria (Daucus carota)
Agrobacterium rhizogenes
hongos micorrizó-genos arbusculares (HMA)
raíces transformadas
kudzú (Pueraria phaseoloides)
carrot (Daucus carota)
Agrobacterium rhizogenes
arbuscular mycorrhizal fungi (AMF)
transformed roots
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_e0ffabb0aafc1d5de820030395aa87b0
oai_identifier_str oai:repositorio.unal.edu.co:unal/40942
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
title Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
spellingShingle Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
kudzú (Pueraria phaseoloides)
zanahoria (Daucus carota)
Agrobacterium rhizogenes
hongos micorrizó-genos arbusculares (HMA)
raíces transformadas
kudzú (Pueraria phaseoloides)
carrot (Daucus carota)
Agrobacterium rhizogenes
arbuscular mycorrhizal fungi (AMF)
transformed roots
title_short Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
title_full Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
title_fullStr Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
title_full_unstemmed Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
title_sort Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
dc.creator.fl_str_mv Medina Sierra, Marisol
Orozco P., Francisco Hernando
Márquez F., María Elena
dc.contributor.author.spa.fl_str_mv Medina Sierra, Marisol
Orozco P., Francisco Hernando
Márquez F., María Elena
dc.subject.proposal.spa.fl_str_mv kudzú (Pueraria phaseoloides)
zanahoria (Daucus carota)
Agrobacterium rhizogenes
hongos micorrizó-genos arbusculares (HMA)
raíces transformadas
kudzú (Pueraria phaseoloides)
carrot (Daucus carota)
Agrobacterium rhizogenes
arbuscular mycorrhizal fungi (AMF)
transformed roots
topic kudzú (Pueraria phaseoloides)
zanahoria (Daucus carota)
Agrobacterium rhizogenes
hongos micorrizó-genos arbusculares (HMA)
raíces transformadas
kudzú (Pueraria phaseoloides)
carrot (Daucus carota)
Agrobacterium rhizogenes
arbuscular mycorrhizal fungi (AMF)
transformed roots
description Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well as kudzu transformation by A.r.15834 and A.r.1724 strains. Transformed carrot root growth was increased in WM culture medium, whilst transformed kudzu root growth did not increase in either the same medium or in modified MS medium. Transformed carrot roots were used for G. intrarradices increase and sporulation; however, wild AMF strains, isolated from a mining area (the lower Cauca area of Antioquia), did not grow either in roots from this specie or those from kudzu, in spite of this plant having great affinity for wild AMF strains. The results represent an advance in the procedure for DNA isolation and keeping AMF collections, required for other research.
publishDate 2002
dc.date.issued.spa.fl_str_mv 2002
dc.date.accessioned.spa.fl_str_mv 2019-06-28T09:44:13Z
dc.date.available.spa.fl_str_mv 2019-06-28T09:44:13Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/40942
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/31039/
url https://repositorio.unal.edu.co/handle/unal/40942
http://bdigital.unal.edu.co/31039/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/biotecnologia/article/view/30109
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología
Revista Colombiana de Biotecnología
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475
dc.relation.references.spa.fl_str_mv Medina Sierra, Marisol and Orozco P., Francisco Hernando and Márquez F., María Elena (2002) Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth. Revista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/40942/1/30109-108392-1-PB.pdf
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Medina Sierra, Marisole0534975-de75-42e4-9061-6218d8da2bb5300Orozco P., Francisco Hernandod4ebc6c9-3217-4103-b76e-475d1ce97855300Márquez F., María Elena7573010b-8def-4ba1-8923-f66083bee3383002019-06-28T09:44:13Z2019-06-28T09:44:13Z2002https://repositorio.unal.edu.co/handle/unal/40942http://bdigital.unal.edu.co/31039/Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well as kudzu transformation by A.r.15834 and A.r.1724 strains. Transformed carrot root growth was increased in WM culture medium, whilst transformed kudzu root growth did not increase in either the same medium or in modified MS medium. Transformed carrot roots were used for G. intrarradices increase and sporulation; however, wild AMF strains, isolated from a mining area (the lower Cauca area of Antioquia), did not grow either in roots from this specie or those from kudzu, in spite of this plant having great affinity for wild AMF strains. The results represent an advance in the procedure for DNA isolation and keeping AMF collections, required for other research.En el presente trabajo se transformaron raíces de kudzú (Pueraria phaseoloides) y de zanahoria (Daucus carota) en diferentes medios de cultivo, mediante el empleo de cinco cepas diferentes de Agrobacterium rhizogenes; de comportamiento diferente tanto en la transformación de zanahoria por las cepas de A. rhizogenes A.r.15834, A.r.8196 y A.r.2659; como en la transformación de kudzú por las cepas A.r.15834 y A.r.1724. Por otro lado, se logró la multiplicación en medio White modificado (WM) de las raíces transformadas de zanahoria, mientras que las de kudzú no se lograron multiplicar en ese medio ni tampoco en el medio MS modificado. Las raíces de zanahoria se emplearon para la multiplicación y esporulación del hongo micorrizógeno arbuscular (HMA) Glomus intrarradices, sin embargo las cepas nativas de HMA, aisladas de una de las zonas de minería del Bajo Cauca Antioqueño, no crecieron en las raíces transformadas de esta especie ni aún en las de kudzú, a pesar de ser una planta que presenta alta afinidad por las cepas nativas de HMA. Los resultados se muestran como un avance en el procedimiento para el aislamiento de ADN y el mantenimiento de colecciones de HMA, requeridos para otras investigaciones.application/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/30109Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de BiotecnologíaRevista Colombiana de BiotecnologíaRevista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475Medina Sierra, Marisol and Orozco P., Francisco Hernando and Márquez F., María Elena (2002) Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth. Revista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475 .Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growthArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTkudzú (Pueraria phaseoloides)zanahoria (Daucus carota)Agrobacterium rhizogeneshongos micorrizó-genos arbusculares (HMA)raíces transformadaskudzú (Pueraria phaseoloides)carrot (Daucus carota)Agrobacterium rhizogenesarbuscular mycorrhizal fungi (AMF)transformed rootsORIGINAL30109-108392-1-PB.pdfapplication/pdf166874https://repositorio.unal.edu.co/bitstream/unal/40942/1/30109-108392-1-PB.pdf95a5cb6a1638741f5ab3a58d13510025MD51THUMBNAIL30109-108392-1-PB.pdf.jpg30109-108392-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8012https://repositorio.unal.edu.co/bitstream/unal/40942/2/30109-108392-1-PB.pdf.jpgf532dd8766a7a542e1f9add8a261188cMD52unal/40942oai:repositorio.unal.edu.co:unal/409422024-01-27 23:06:35.454Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co