Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth
Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well a...
- Autores:
-
Medina Sierra, Marisol
Orozco P., Francisco Hernando
Márquez F., María Elena
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2002
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/40942
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/40942
http://bdigital.unal.edu.co/31039/
- Palabra clave:
- kudzú (Pueraria phaseoloides)
zanahoria (Daucus carota)
Agrobacterium rhizogenes
hongos micorrizó-genos arbusculares (HMA)
raíces transformadas
kudzú (Pueraria phaseoloides)
carrot (Daucus carota)
Agrobacterium rhizogenes
arbuscular mycorrhizal fungi (AMF)
transformed roots
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
id |
UNACIONAL2_e0ffabb0aafc1d5de820030395aa87b0 |
---|---|
oai_identifier_str |
oai:repositorio.unal.edu.co:unal/40942 |
network_acronym_str |
UNACIONAL2 |
network_name_str |
Universidad Nacional de Colombia |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
title |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
spellingShingle |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth kudzú (Pueraria phaseoloides) zanahoria (Daucus carota) Agrobacterium rhizogenes hongos micorrizó-genos arbusculares (HMA) raíces transformadas kudzú (Pueraria phaseoloides) carrot (Daucus carota) Agrobacterium rhizogenes arbuscular mycorrhizal fungi (AMF) transformed roots |
title_short |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
title_full |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
title_fullStr |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
title_full_unstemmed |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
title_sort |
Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth |
dc.creator.fl_str_mv |
Medina Sierra, Marisol Orozco P., Francisco Hernando Márquez F., María Elena |
dc.contributor.author.spa.fl_str_mv |
Medina Sierra, Marisol Orozco P., Francisco Hernando Márquez F., María Elena |
dc.subject.proposal.spa.fl_str_mv |
kudzú (Pueraria phaseoloides) zanahoria (Daucus carota) Agrobacterium rhizogenes hongos micorrizó-genos arbusculares (HMA) raíces transformadas kudzú (Pueraria phaseoloides) carrot (Daucus carota) Agrobacterium rhizogenes arbuscular mycorrhizal fungi (AMF) transformed roots |
topic |
kudzú (Pueraria phaseoloides) zanahoria (Daucus carota) Agrobacterium rhizogenes hongos micorrizó-genos arbusculares (HMA) raíces transformadas kudzú (Pueraria phaseoloides) carrot (Daucus carota) Agrobacterium rhizogenes arbuscular mycorrhizal fungi (AMF) transformed roots |
description |
Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well as kudzu transformation by A.r.15834 and A.r.1724 strains. Transformed carrot root growth was increased in WM culture medium, whilst transformed kudzu root growth did not increase in either the same medium or in modified MS medium. Transformed carrot roots were used for G. intrarradices increase and sporulation; however, wild AMF strains, isolated from a mining area (the lower Cauca area of Antioquia), did not grow either in roots from this specie or those from kudzu, in spite of this plant having great affinity for wild AMF strains. The results represent an advance in the procedure for DNA isolation and keeping AMF collections, required for other research. |
publishDate |
2002 |
dc.date.issued.spa.fl_str_mv |
2002 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-28T09:44:13Z |
dc.date.available.spa.fl_str_mv |
2019-06-28T09:44:13Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/40942 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/31039/ |
url |
https://repositorio.unal.edu.co/handle/unal/40942 http://bdigital.unal.edu.co/31039/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/biotecnologia/article/view/30109 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología Revista Colombiana de Biotecnología |
dc.relation.ispartofseries.none.fl_str_mv |
Revista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475 |
dc.relation.references.spa.fl_str_mv |
Medina Sierra, Marisol and Orozco P., Francisco Hernando and Márquez F., María Elena (2002) Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth. Revista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/40942/1/30109-108392-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/40942/2/30109-108392-1-PB.pdf.jpg |
bitstream.checksum.fl_str_mv |
95a5cb6a1638741f5ab3a58d13510025 f532dd8766a7a542e1f9add8a261188c |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
_version_ |
1814090184043528192 |
spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Medina Sierra, Marisole0534975-de75-42e4-9061-6218d8da2bb5300Orozco P., Francisco Hernandod4ebc6c9-3217-4103-b76e-475d1ce97855300Márquez F., María Elena7573010b-8def-4ba1-8923-f66083bee3383002019-06-28T09:44:13Z2019-06-28T09:44:13Z2002https://repositorio.unal.edu.co/handle/unal/40942http://bdigital.unal.edu.co/31039/Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well as kudzu transformation by A.r.15834 and A.r.1724 strains. Transformed carrot root growth was increased in WM culture medium, whilst transformed kudzu root growth did not increase in either the same medium or in modified MS medium. Transformed carrot roots were used for G. intrarradices increase and sporulation; however, wild AMF strains, isolated from a mining area (the lower Cauca area of Antioquia), did not grow either in roots from this specie or those from kudzu, in spite of this plant having great affinity for wild AMF strains. The results represent an advance in the procedure for DNA isolation and keeping AMF collections, required for other research.En el presente trabajo se transformaron raíces de kudzú (Pueraria phaseoloides) y de zanahoria (Daucus carota) en diferentes medios de cultivo, mediante el empleo de cinco cepas diferentes de Agrobacterium rhizogenes; de comportamiento diferente tanto en la transformación de zanahoria por las cepas de A. rhizogenes A.r.15834, A.r.8196 y A.r.2659; como en la transformación de kudzú por las cepas A.r.15834 y A.r.1724. Por otro lado, se logró la multiplicación en medio White modificado (WM) de las raíces transformadas de zanahoria, mientras que las de kudzú no se lograron multiplicar en ese medio ni tampoco en el medio MS modificado. Las raíces de zanahoria se emplearon para la multiplicación y esporulación del hongo micorrizógeno arbuscular (HMA) Glomus intrarradices, sin embargo las cepas nativas de HMA, aisladas de una de las zonas de minería del Bajo Cauca Antioqueño, no crecieron en las raíces transformadas de esta especie ni aún en las de kudzú, a pesar de ser una planta que presenta alta afinidad por las cepas nativas de HMA. Los resultados se muestran como un avance en el procedimiento para el aislamiento de ADN y el mantenimiento de colecciones de HMA, requeridos para otras investigaciones.application/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/30109Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de BiotecnologíaRevista Colombiana de BiotecnologíaRevista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475Medina Sierra, Marisol and Orozco P., Francisco Hernando and Márquez F., María Elena (2002) Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growth. Revista Colombiana de Biotecnología; Vol. 4, núm. 2 (2002); 52-57 1909-8758 0123-3475 .Advances in transforming kudzu (pueraria phaseoloides) and carrot (daucus carota var. danvers 126) roots with different agrobacterium rhizogenes strains for increasing ma fungi growthArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTkudzú (Pueraria phaseoloides)zanahoria (Daucus carota)Agrobacterium rhizogeneshongos micorrizó-genos arbusculares (HMA)raíces transformadaskudzú (Pueraria phaseoloides)carrot (Daucus carota)Agrobacterium rhizogenesarbuscular mycorrhizal fungi (AMF)transformed rootsORIGINAL30109-108392-1-PB.pdfapplication/pdf166874https://repositorio.unal.edu.co/bitstream/unal/40942/1/30109-108392-1-PB.pdf95a5cb6a1638741f5ab3a58d13510025MD51THUMBNAIL30109-108392-1-PB.pdf.jpg30109-108392-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8012https://repositorio.unal.edu.co/bitstream/unal/40942/2/30109-108392-1-PB.pdf.jpgf532dd8766a7a542e1f9add8a261188cMD52unal/40942oai:repositorio.unal.edu.co:unal/409422024-01-27 23:06:35.454Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |