Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus
ilustraciones, diagramas, fotografías, mapas
- Autores:
-
Romero Benavides, Dario Alejandro
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/86574
- Palabra clave:
- 570 - Biología::577 - Ecología
590 - Animales::599 - Mamíferos
Flora intestinal
Cavia
Ciego
intestinal flora
Cavia
cecum
Cavia porcellus
Nariño
Microbioma
Ciego
Bioinformática
Cavia porcellus
Nariño
Cecum
Bioinformatics
Microbiome
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| dc.title.translated.eng.fl_str_mv |
Advances in the investigation of intestinal bacterial populations of Cavia porcellus |
| title |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| spellingShingle |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus 570 - Biología::577 - Ecología 590 - Animales::599 - Mamíferos Flora intestinal Cavia Ciego intestinal flora Cavia cecum Cavia porcellus Nariño Microbioma Ciego Bioinformática Cavia porcellus Nariño Cecum Bioinformatics Microbiome |
| title_short |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| title_full |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| title_fullStr |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| title_full_unstemmed |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| title_sort |
Avance en la investigación de poblaciones bacterianas intestinales del Cavia porcellus |
| dc.creator.fl_str_mv |
Romero Benavides, Dario Alejandro |
| dc.contributor.advisor.spa.fl_str_mv |
Caro Quintero, Alejandro |
| dc.contributor.author.spa.fl_str_mv |
Romero Benavides, Dario Alejandro |
| dc.contributor.subjectmatterexpert.spa.fl_str_mv |
Rios de Álvarez, Leyla Chaves, Carlos Alberto |
| dc.contributor.orcid.spa.fl_str_mv |
Romero Benavides, Dario Alejandro [000000020831183] |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::577 - Ecología 590 - Animales::599 - Mamíferos |
| topic |
570 - Biología::577 - Ecología 590 - Animales::599 - Mamíferos Flora intestinal Cavia Ciego intestinal flora Cavia cecum Cavia porcellus Nariño Microbioma Ciego Bioinformática Cavia porcellus Nariño Cecum Bioinformatics Microbiome |
| dc.subject.agrovoc.spa.fl_str_mv |
Flora intestinal Cavia Ciego |
| dc.subject.agrovoc.eng.fl_str_mv |
intestinal flora Cavia cecum |
| dc.subject.proposal.spa.fl_str_mv |
Cavia porcellus Nariño Microbioma Ciego Bioinformática |
| dc.subject.proposal.eng.fl_str_mv |
Cavia porcellus Nariño Cecum Bioinformatics Microbiome |
| description |
ilustraciones, diagramas, fotografías, mapas |
| publishDate |
2024 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-19T14:14:28Z |
| dc.date.available.none.fl_str_mv |
2024-07-19T14:14:28Z |
| dc.date.issued.none.fl_str_mv |
2024 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
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info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/86574 |
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Universidad Nacional de Colombia |
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Repositorio Institucional Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/ |
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https://repositorio.unal.edu.co/handle/unal/86574 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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Agrosavia Agrovoc |
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(2013). High-throughput 16S rRNA gene sequencing reveals alterations of intestinal microbiota in myalgic encephalomyelitis/chronic fatigue syndrome patients. Anaerobe, 22, 50–56. https://doi.org/10.1016/j.anaerobe.2013.06.002 Zhang, X., Zhang, D., Jia, H., Feng, Q., Wang, D., Liang, D., Wu, X., Li, J., Tang, L., Li, Y., Lan, Z., Chen, B., Li, Y., Zhong, H., Xie, H., Jie, Z., Chen, W., Tang, S., Xu, X., … Wang, J. (2015). The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment. Nature Medicine, 21(8), 895–905. https://doi.org/10.1038/nm.3914 Brown, C. T., Davis-Richardson, A. G., Giongo, A., Gano, K. A., Crabb, D. B., Mukherjee, N., ... & Triplett, E. W. (2011). Gut microbiome metagenomics analysis suggests a functional model for the development of autoimmunity for type 1 diabetes. PloS one, 6(10), e25792. DOI: 10.1371/journal.pone.0025792 Ferreira-Halder, C. V., de Sousa Faria, A. V., & Andrade, S. S. (2017). Action and function of Faecalibacterium prausnitzii in health and disease. Best Practice & Research Clinical Gastroenterology, 31(6), 643-648. OI: 10.1016/j.bpg.2017.09.011 Newbold CJ, Ramos-Morales E. (2020). Review: Ruminal microbiome and microbial metabolome: effects of diet and ruminant host. Animal. Mar;14(S1):s78-s86. doi: 10.1017/S1751731119003252. PMID: 32024572 Henderson, G., Cox, F., Ganesh, S., Jonker, A., Young, W., & Janssen, P. H. (2015). Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Scientific reports, 5(1), 14567. https://doi.org/10.1038/srep14567 Hildebrand, F., Ebersbach, T., Nielsen, H. B., Li, X., Sonne, S. B., Bertalan, M., ... & Licht, T. R. (2012). A comparative analysis of the intestinal metagenomes present in guinea pigs (Cavia porcellus) and humans (Homo sapiens). BMC genomics, 13(1), 1-11. https://doi.org/10.1186/1471-2164-13-514 Caycedo, A. (2000). Experiencias Investigativas en la Producción de Cuyes (Facultad de Ciencias pecuarias (ed.); 1 ed). Contribución al desarrollo tecnológico de la explotación Palakawong Na Ayudthaya, S., van der Oost, H., van der Oost, J., van Vliet, D. M., & Plugge, C. M. (2019). Microbial Diversity and Organic Acid Production of Guinea Pig Faecal Samples. Current Microbiology, 76, 425-434. doi: 10.1007/s00284-019-01630-x Gobernación de Nariño, Agencia de Desarrollo Rural (ADR), Organización de las Naciones Unidas para la alimentación y la Agricultura (FAO), and Ministerio de Agricultura y Desarrollo Rural (MADR). (2019). II Plan Integral de Desarrollo Agropecuario y Rural Con Enfoque Territorial: Departamento de Nariño Avilés Esquivel, D. (2016). Caracterización genética del cuy doméstico de América del Sur mediante marcadores moleculares. [Tesis doctoral, Universidad de Córdoba]. Repositorio institucional de la Universidad de Córdoba https://helvia.uco.es/handle/10396/13382 Patiño Burbano RE, Cardona-Iglesias JL, Carlosama-Ojeda LD, Portillo-Lopez PA, Moreno DC. (2019). Zootechnical parameters of Cavia porcellus in production systems in Nariño and Putumayo (Colombia). CES Medicina Veterinaria y Zootecnia, 14(3), 29-41 Secretaría de Agricultura y Desarrollo Rural, Universidad Sergio Arboleda. (2017). Consolidado Agropecuario del departamento de Nariño. Pritt, S. (2012). Taxonomy and history. In The laboratory rabbit, guinea pig, hamster, and other rodents (pp. 563-574). Academic Press Shomer, N. H., Holcombe, H., & Harkness, J. E. (2015). Biology and diseases of guinea pigs. In Laboratory animal medicine (pp. 247-283). Academic Press. Hargaden, M., & Singer, L. (2012). Anatomy, physiology, and behavior. In The laboratory rabbit, Guinea pig, hamster, and other rodents (pp. 575-602). Academic Press. Imam, J., Hambolu, J. O., Onyeanusi, B. I., Ayo, J. O., & Sulaiman, M. H. (2021). Morphological and Morphometric Studies of the Gastro-intestinal Tract of the Guinea Pig (Cavia porcellus-Linnaeus, 1758). Journal of Veterinary Anatomy, 14(1). doi: 10.21608/JVA.2021.163576. Stan, F. G. (2018). A Comparison Between the Macroscopic Anatomy of the Cecum in Laboratory Rat and Guinea Pig. Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 75(1). doi:10.15835/buasvmcn-vm:002817. Frias, H., Valderrama, N. L. M., Flores, G. J., Cornejo, V. G., Del Solar, J. C., Romani, A. C., ... & Quintana, J. L. M. (2023a). An analysis of the cecum microbiome of three breeds of the guinea pig: Andina, inti, and Peru. Research in Veterinary Science. DOI: 10.1016/j.rvsc.2023.06.005. Sandoval Alarcón, H. F. (2013). Evaluación de diferentes tipos de dietas en cobayos en crecimiento [Tesis pregrado, Universidad Técnica de Ambato]. Repositorio institucional de la Universidad Técnica de Ambato https://repositorio.uta.edu.ec/handle/123456789/5224. Takahashi, T., Karita, S., Yahaya, M. S., & Goto, M. (2005). Radial and Axial Variations of Bacteria within the Cecum and Proximal Colon of Guinea Pigs Revealed by PCR–DGGE. Bioscience, biotechnology, and biochemistry, 69(9), 1790-1792. Lecuit, M., Dramsi, S., Gottardi, C., Fedor‐Chaiken, M., Gumbiner, B., & Cossart, P. (1999). A single amino acid in E‐cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes. The EMBO journal. 15;18(14):3956-63. doi: 10.1093/emboj/18.14.3956. Crowley, E. J., King, J. M., Wilkinson, T., Worgan, H. J., Huson, K. M., Rose, M. T., & McEwan, N. R. (2017). Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing. PloS one, 12(2), e0165779. https://doi.org/10.1371/journal.pone.0165779. Tang, C., Ma, J., Kong, F., Li, B., Du, Q., Zhang, Y., ... & Li, M. (2022). The Analysis of Transcriptomes and Microorganisms Reveals Differences between the Intestinal Segments of Guinea Pigs. Animals, 12(21), 2925. https://doi.org/10.3390/ani12212925. Kers, J. G., Velkers, F. C., Fischer, E. A., Hermes, G. D., Lamot, D. M., Stegeman, J. A., & Smidt, H. (2019). Take care of the environment: housing conditions affect the interplay of nutritional interventions and intestinal microbiota in broiler chickens. Animal Microbiome, 1, 1-14. https://doi.org/10.1186/s42523-019-0009-z. National Research Council. (2017). Guía para el cuidado y uso de animales de laboratorio. Ediciones UC. Ley 84, diciembre 27 de 1989. El Congreso de Colombia. Por la cual se adopta el Estatuto Nacional de Protección de los Animales y se crean unas contravenciones y se regula lo referente a su procedimiento y competencia. Ley 1774, enero 6 de 2016. El Congreso de Colombia. 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A review of Ureaplasma diversum: a representative of the Mollicute class associated with reproductive and respiratory disorders in cattle. Frontiers in veterinary science, 8, 572171. Miyake, S., Ngugi, D. K., & Stingl, U. (2016). Phylogenetic diversity, distribution, and cophylogeny of giant bacteria (Epulopiscium) with their surgeonfish hosts in the Red Sea. Frontiers in microbiology, 7, 285. Yang, J., Li, Y., Wen, Z., Liu, W., Meng, L., & Huang, H. (2021). Oscillospira-a candidate for the next-generation probiotics. Gut microbes, 13(1), 1987783. Maier, T. V., Lucio, M., Lee, L. H., VerBerkmoes, N. C., Brislawn, C. J., Bernhardt, J., ... & Jansson, J. K. (2017). Impact of dietary resistant starch on the human gut microbiome, metaproteome, and metabolome. MBio, 8(5), 10-1128. Hongoh, Y., Deevong, P., Hattori, S., Inoue, T., Noda, S., Noparatnaraporn, N., ... & Ohkuma, M. (2006). Phylogenetic diversity, localization, and cell morphologies of members of the candidate phylum TG3 and a subphylum in the phylum Fibrobacteres, recently discovered bacterial groups dominant in termite guts. Applied and Environmental Microbiology, 72(10), 6780-6788. Ransom-Jones, E., Jones, D. L., McCarthy, A. J., & McDonald, J. E. (2012). The Fibrobacteres: an important phylum of cellulose-degrading bacteria. Microbial ecology, 63, 267-281. Goertz, S., de Menezes, A. B., Birtles, R. J., Fenn, J., Lowe, A. E., MacColl, A. D., ... & Taylor, C. H. (2019). Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale. PloS one, 14(9), e0222501. https://doi.org/10.1371/journal.pone.0222501. Trujillo, S. M., McKenney, E. A., Hilderbrand, G. V., Mangipane, L. S., Rogers, M. C., Joly, K., ... & Lafferty, D. J. (2022). Intrinsic and extrinsic factors influence on an omnivore’s gut microbiome. PloS one, 17(4), e0266698. Dao, T. K., Do, T. H., Le, N. G., Nguyen, H. D., Nguyen, T. Q., Le, T. T. H., & Truong, N. H. (2021). Understanding the role of Prevotella genus in the digestion of lignocellulose and other substrates in Vietnamese native goats’ rumen by metagenomic deep sequencing. Animals, 11(11), 3257. Narayanasamy, S., Chan, K. L., Cai, H., Abdul Razak, A. H. B., Tay, B. K., & Miao, H. (2020). Biobutanol production from sugarcane bagasse by Clostridium beijerinckii strains. Biotechnology and Applied Biochemistry, 67(5), 732-737. Frias, H., Valderrama, N. L. M., Durand, G. J. F., Cornejo, V. G., Romani, A. C., Bardales, W., ... & Quintana, J. L. M. (2023b). Comparative analysis of fasting effects on the cecum microbiome in three guinea pig breeds: Andina, Inti, and Peru. Frontiers in Microbiology, 14. doi: 10.3389/fmicb.2023.1283738. Wang, Z., Zhang, C., Li, G., & Yi, X. (2022). The influence of species identity and geographic locations on gut microbiota of small rodents. Frontiers in Microbiology, 13, 983660. https://doi.org/10.3389/fmicb.2022.983660. Wu, X., Wei, Q., Wang, X., Shang, Y., & Zhang, H. (2022). Evolutionary and dietary relationships of wild mammals based on the gut microbiome. Gene, 808, 145999. https://doi.org/10.1016/j.gene.2021.145999. Hu, X., Liu, G., Shafer, A. B., Wei, Y., Zhou, J., Lin, S., ... & Liu, S. (2017). Comparative analysis of the gut microbial communities in forest and alpine musk deer using high-throughput sequencing. Frontiers in Microbiology, 8, 572. https://doi.org/10.3389/fmicb.2017.00572. Bensch, H. M., Tolf, C., Waldenström, J., Lundin, D., & Zöttl, M. (2023). Bacteroidetes to Firmicutes: captivity changes the gut microbiota composition and diversity in a social subterranean rodent. Animal Microbiome, 5(1), 1-11. https://doi.org/10.1186/s42523-023-00231-1. Brabb, T., Newsome, D., Burich, A., & Hanes, M. (2012). Infectious diseases. In: The laboratory rabbit, guinea pig, hamster, and other rodents, 637-683. Bowerman, K. L., Knowles, S. C., Bradley, J. E., Baltrūnaitė, L., Lynch, M. D., Jones, K. M., & Hugenholtz, P. (2021). Effects of laboratory domestication on the rodent gut microbiome. ISME communications, 1(1), 49. https://doi.org/10.1038/s43705-021-00053-9. Warriner, K., & Namvar, A. (2017). Current Challenges in Enhancing the Microbiological Safety of Raw Meat. In New aspects of meat quality (pp. 191-222). Woodhead Publishing. Collado, L. (2020). Diagnóstico microbiológico y vigilancia epidemiológica de la campilobacteriosis en Chile: Situación actual y desafíos futuros. Revista chilena de infectología, 37(3), 244-251. ACHIPIA. Agencia Chilena para la Inocuidad y Calidad Alimentaria. 2017. Campylobacter spp. McCallum, G., & Tropini, C. (2023). The gut microbiota and its biogeography. Nature Reviews Microbiology, 1-14. https://doi.org/10.1038/s41579-023-00969-0. Tournelle Kimura, P. M. (2013). Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries. [Tesis cirujano dentista, Universidad de Chile] Repositorio https://repositorio.uchile.cl/handle/2250/117563. Meseguer-Peinado, M. A., Acosta-Boga, B., Matas-Andreu, L., & Codina-Grau, G. (2012). Diagnóstico microbiológico de las infecciones por Mycoplasma. Enfermedades Infecciosas y Microbiología Clínica, 30(8), 500-504. Swanson, M. T., Henson, M. W., Handika, H., Achmadi, A. S., Anita, S., Rowe, K. C., & Esselstyn, J. A. (2023). Mycoplasmataceae dominate microbial community differences between gut regions in mammals with a simple gut architecture. Journal of Mammalogy, 104(1), 146-158. Abdallah, F., & Gharib, H. S. (2024). Studying caecotrophy behaviour (complete and censored) in three rabbit breeds at four seasons using different survival techniques. Assiut Veterinary Medical Journal, 70(180), 26-37. |
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Universidad Nacional de Colombia |
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Bogotá - Ciencias - Maestría en Ciencias - Microbiología |
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Facultad de Ciencias |
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Bogotá, Colombia |
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Universidad Nacional de Colombia - Sede Bogotá |
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Universidad Nacional de Colombia |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Caro Quintero, Alejandro88acb35536fdbb1969fe4b20b77cdf3f600Romero Benavides, Dario Alejandrof4f73051e27bcc112e6af611dbe5e6e7600Rios de Álvarez, LeylaChaves, Carlos AlbertoRomero Benavides, Dario Alejandro [000000020831183]2024-07-19T14:14:28Z2024-07-19T14:14:28Z2024https://repositorio.unal.edu.co/handle/unal/86574Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías, mapasEl cuy (Cavia porcellus) es un pequeño mamífero monogástrico de la familia Caviidae originario de los Andes. Esta especie está distribuida en varios países de Sudamérica, donde ha ganado reconocimiento por su aptitud como especie de producción pecuaria promisoria y emergente. En Colombia, la cría de la especie se encuentra principalmente en el departamento de Nariño, lugar en donde forma parte importante de la cultura, diversidad gastronómica y el sustento económico de muchas familias tanto en el sector urbano como rural. Esta investigación, da a conocer la primera caracterización del tracto gastrointestinal (TGI) del Cavia porcellus realizada en el municipio de Pasto, la cual toma como eje principal el ciego de los animales del sector productivo. Se enfoca en explorar una parte de la ecología bacteriana intestinal de la especie, la cual ha sido escasamente estudiada y tiene una profunda conexión en aspectos ecológicos y biológicos de la especie. Con este propósito, 288 cuyes (15-20 días de edad, exentos de antibióticos y alimento balanceado) fueron seleccionados de 14 de los 17 corregimientos de Pasto. De cada animal se obtuvieron muestras de segmentos del TGI y heces para construir 327 librerías (288: ciego, 29: tejidos) basadas en el gen ARNr 16S – V4. En cada lugar de muestreo (unidad productiva - UP) se realizó el levantamiento sistemático de información que permitió la caracterización de la producción de cuy en Pasto, y fue la base de la meta información empleada en el análisis bioinformático. Globalmente, este estudio determinó que las mujeres lideran la producción de cuy en Pasto (84%); el 64.9% de los productores tiene la capacidad de producir entre 41-200 animales/UP; en el 100% de las UP se utilizan pastos y forrajes como alimento principal y el 65.5% de los productores producen el pasto que suministran a los animales. En el aspecto sanitario, en el 72.2% de las UP se suministran antiparasitarios y en el 23.7 % se administran productos que contienen Enrofloxacina. Las variables con mayor peso sobre los índices de diversidad alfa en el ciego fueron la distancia geográfica (corregimiento), el uso de antiparasitarios, la presencia de otras especies y el tipo de forraje utilizado. Por su parte, a nivel de segmentos y heces, las variables género (machos y hembras) y segmento (duodeno, yeyuno, íleon, colon y heces) fueron las que mayormente contribuyen con las diferencias significativas en cuanto a su alfa diversidad. El análisis de beta diversidad (PERMANOVA y PcoA Unifrac ponderado), identificaron que la diversidad bacteriana del ciego está fuertemente influenciada por la distancia geográfica de los corregimientos y por las unidades productivas. La asignación taxonómica se identificó 14 filo en las muestras de ciego; Firmicutes y Bacteroidetes fueron los filos con mayor abundancia en las muestras. En cuanto a la significancia biológica, se encontró que está representada por 3 filos con LDA score < 2 (Firmicutes, Bacteroidetes y Fibrobacteres). La beta diversidad del TGI, (PERMANOVA y PcoA Unifrac ponderado y no ponderado), identificó una marcada separación entre los segmentos del intestino delgado (ID) el intestino grueso (IG) y heces. A su vez identifico que los segmentos del ID son más similares entre ellos al igual que los segmentos del IG y las heces. Un patrón de expresión diferencial interesante surgió entre los segmentos del ID y el IG y las heces (LEfSe); cuando un filo se registró con un valor LDA score > 2, en cualquier segmento del ID, de forma opuesta se observó su baja expresión en todos los segmentos del IG y las heces. Este patrón se también se identificó de forma contraria. Finalmente, es posible concluir que el bacterioma intestinal del Cavia porcellus difiere en relación a los segmentos que lo componen. Especialmente el ciego, es una estructura que aloja un bacterioma relativamente estable, pero que puede ser afectado en mayor o menor grado por las variables que confluyen en los sistemas productivos. (Texto tomado de la fuente).The guinea pig (Cavia porcellus) is a small monogastric mammal of the Caviidae family, native to the Andes. This species is distributed in several countries in South America, where it has gained recognition for its suitability as a promising and emerging livestock production species. In Colombia, the breeding of the species is mainly found in the department of Nariño, a place where it forms an important part of the culture, gastronomic diversity, and the economic sustenance of many families in both the urban and rural sectors. This research reveals the first characterization of the gastrointestinal tract (GIT) of Cavia porcellus carried out in the municipality of Pasto, which takes as its main focus the cecum of animals in the productive sector. It focuses on exploring a part of the intestinal bacterial ecology of the species, which has been poorly studied and has a deep connection to the ecological and biological aspects of the species. For this purpose, 288 guinea pigs (15-20 days old, free of antibiotics and balanced food) were selected from 14 of the 17 towns of Pasto. Samples of GIT segments and feces were obtained from each animal to construct 327 libraries (288: cecum, 29: tissues) based on the 16S rRNA gene – V4. In each sampling location (productive unit), a systematic collection of information was carried out that allowed the characterization of guinea pig production in Pasto and served as the basis for the meta-information used in the bioinformatic analysis. Globally, this study determined that women lead guinea pig production in Pasto (84%); 64.9% of producers have the capacity to produce between 41-200 animals per productive unit. In 100% of the productive units, grasses and forage are used as the main food, and 65.5% of the producers grow the grass that they supply to the animals. In terms of health, 72.2% of the productive units administer antiparasitics, and in 23.7% products containing enrofloxacin are administered. The variables with the greatest weight on the alpha diversity indices in the cecum were geographical distance (township), the use of antiparasitics, the presence of other species, and the type of forage used. For its part, at the level of segments and feces, the variables gender (males and females) and segment (duodenum, jejunum, ileum, colon, and feces) were the ones that mostly contributed to the significant differences in terms of their alpha diversity. The beta diversity analysis (PERMANOVA and weighted PCoA Unifrac) identified that the bacterial diversity of the cecum, is strongly influenced by the geographical distance of the townships and by the productive units. Taxonomic assignment was identified to 14 phyla in cecum samples; Firmicutes and Bacteroidetes were the phyla with the highest abundance in the samples. Regarding biological significance, it was found that it is represented by 3 phyla with an LDA score < 2 (Firmicutes, Bacteroidetes, and Fibrobacteres). The beta diversity of the GIT (PERMANOVA and weighted and unweighted Unifrac PCoA) identified a marked separation between the segments of the small intestine (SI), the large intestine (LI), and feces. At the same time, it identified that the SI segments are more similar to each other, as are the LI segments and feces. An interesting differential expression pattern emerged between the SI and LI segments and feces (LEfSe). When a phylum was recorded with an LDA score value > 2 in any segment of the SI, its low expression was observed in all segments of the LI and feces. This pattern was also identified in the opposite way. Finally, it is possible to conclude that the intestinal bacteriome of Cavia porcellus differs in relation to the segments that compose it. The cecum, in particular, houses a relatively stable bacteriome, but it can be affected to a greater or lesser degree by the variables present in production systems.Dirección Nacional de Investigación y Laboratorios - Vicerrectoría de InvestigaciónMaestríaMagíster en Ciencias - Microbiologíaxv, 111 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::577 - Ecología590 - Animales::599 - MamíferosFlora intestinalCaviaCiegointestinal floraCaviacecumCavia porcellusNariñoMicrobiomaCiegoBioinformáticaCavia porcellusNariñoCecumBioinformaticsMicrobiomeAvance en la investigación de poblaciones bacterianas intestinales del Cavia porcellusAdvances in the investigation of intestinal bacterial populations of Cavia porcellusTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAgrosaviaAgrovocFranklin, C. 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Assiut Veterinary Medical Journal, 70(180), 26-37.Convocatoria Nacional para el Fortalecimiento de la Formación a través del Apoyo a Proyectos de Investigación, Creación Artística o Innovación de la Universidad Nacional de Colombia 2022-2024 SemillerosEstudiantesGrupos comunitariosInvestigadoresMaestrosMedios de comunicaciónPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86574/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1085249421.2024.pdf1085249421.2024.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf3055031https://repositorio.unal.edu.co/bitstream/unal/86574/3/1085249421.2024.pdff2fae3c98c738bac60750644241c5cf0MD53THUMBNAIL1085249421.2024.pdf.jpg1085249421.2024.pdf.jpgGenerated Thumbnailimage/jpeg4643https://repositorio.unal.edu.co/bitstream/unal/86574/4/1085249421.2024.pdf.jpg4875a25613e43fef850a447145268b1aMD54unal/86574oai:repositorio.unal.edu.co:unal/865742024-08-26 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