Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron
The Meliacea are of great importance in construction and furniture-making. Toona ciliata is a Meliacea originally from the Himalayan region; it is known as Himalayan Cedar in Cuba. Natural regeneration occurs in this specie by seed diffusion and grafting; such propagation is limited. The object of t...
- Autores:
-
Daquinta, Marcos
Lezcano, Yarianne
Cid, Mariela
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2005
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/22097
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/22097
http://bdigital.unal.edu.co/13131/
- Palabra clave:
- callus
Himalayan Cedar
Meliacea
growth regulators
plant regeneration
callo
cedro del Himalaya
Meliácea
reguladores del crecimiento
regeneración de plantas
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
dc.title.spa.fl_str_mv |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
title |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
spellingShingle |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron callus Himalayan Cedar Meliacea growth regulators plant regeneration callo cedro del Himalaya Meliácea reguladores del crecimiento regeneración de plantas |
title_short |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
title_full |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
title_fullStr |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
title_full_unstemmed |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
title_sort |
Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
dc.creator.fl_str_mv |
Daquinta, Marcos Lezcano, Yarianne Cid, Mariela |
dc.contributor.author.spa.fl_str_mv |
Daquinta, Marcos Lezcano, Yarianne Cid, Mariela |
dc.subject.proposal.spa.fl_str_mv |
callus Himalayan Cedar Meliacea growth regulators plant regeneration callo cedro del Himalaya Meliácea reguladores del crecimiento regeneración de plantas |
topic |
callus Himalayan Cedar Meliacea growth regulators plant regeneration callo cedro del Himalaya Meliácea reguladores del crecimiento regeneración de plantas |
description |
The Meliacea are of great importance in construction and furniture-making. Toona ciliata is a Meliacea originally from the Himalayan region; it is known as Himalayan Cedar in Cuba. Natural regeneration occurs in this specie by seed diffusion and grafting; such propagation is limited. The object of this paper was to promote callus formation and plant regeneration in Toona ciliata from in vitro propagation. Two to three year old mature trees (Toona ciliata) were used. Rachis were taken from young branches from these plants. They were disinfected in 0.25% (w/v) mercuric chloride solution for 10 min followed by three rinses in autoclaved distilled-water. They were then established in MS supplemented with 0-1 mg/L thidiazuron culture medium. Nodular calluses were obtained having good morphogenic characteristics. Shoots sprouted from six-month-old calluses in the dark and plant regeneration was done in the light. AIB shoots were rooted in MS medium supplement with 1 mg/L IBA. Key words: callus, Himalayan Cedar, Meliacea, growth regulators, plant regeneration. Abbreviations: IBA- indolebutyric acid, thidiazuron-N-1,2,3-thiadiazol-5-yl-N-phenylurea. |
publishDate |
2005 |
dc.date.issued.spa.fl_str_mv |
2005 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-25T20:33:10Z |
dc.date.available.spa.fl_str_mv |
2019-06-25T20:33:10Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/22097 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/13131/ |
url |
https://repositorio.unal.edu.co/handle/unal/22097 http://bdigital.unal.edu.co/13131/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/biotecnologia/article/view/489 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología Revista Colombiana de Biotecnología |
dc.relation.ispartofseries.none.fl_str_mv |
Revista Colombiana de Biotecnología; Vol. 7, núm. 2 (2005); 5-9 1909-8758 0123-3475 |
dc.relation.references.spa.fl_str_mv |
Daquinta, Marcos and Lezcano, Yarianne and Cid, Mariela (2005) Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron. Revista Colombiana de Biotecnología; Vol. 7, núm. 2 (2005); 5-9 1909-8758 0123-3475 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
text/html |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Daquinta, Marcose8319e62-8523-498f-9394-05ba59efd512300Lezcano, Yarianne2049a4a8-40ec-491c-9ed3-456208998980300Cid, Mariela1889d133-d45a-4423-bdbd-b05b716cb69d3002019-06-25T20:33:10Z2019-06-25T20:33:10Z2005https://repositorio.unal.edu.co/handle/unal/22097http://bdigital.unal.edu.co/13131/The Meliacea are of great importance in construction and furniture-making. Toona ciliata is a Meliacea originally from the Himalayan region; it is known as Himalayan Cedar in Cuba. Natural regeneration occurs in this specie by seed diffusion and grafting; such propagation is limited. The object of this paper was to promote callus formation and plant regeneration in Toona ciliata from in vitro propagation. Two to three year old mature trees (Toona ciliata) were used. Rachis were taken from young branches from these plants. They were disinfected in 0.25% (w/v) mercuric chloride solution for 10 min followed by three rinses in autoclaved distilled-water. They were then established in MS supplemented with 0-1 mg/L thidiazuron culture medium. Nodular calluses were obtained having good morphogenic characteristics. Shoots sprouted from six-month-old calluses in the dark and plant regeneration was done in the light. AIB shoots were rooted in MS medium supplement with 1 mg/L IBA. Key words: callus, Himalayan Cedar, Meliacea, growth regulators, plant regeneration. Abbreviations: IBA- indolebutyric acid, thidiazuron-N-1,2,3-thiadiazol-5-yl-N-phenylurea.Las Meliáceas son de gran importancia en la construcción y fabricación de muebles, entre otras aplicaciones. Toona ciliata es una Meliácea originaria de la región del Himalaya; en Cuba se conoce como cedro del Himalaya. La regeneración natural de esta especie es por semillas e injertos. Los procesos de propagación en la producción de esta especie son limitados. El objetivo de este trabajo fue lograr la formación de callos y la regeneración de plantas en Toona ciliata para la propagación in vitro. Se usaron árboles de dos a tres años de Toona ciliata. A partir de estas plantas se utilizaron raquis de hojas jóvenes las cuales se desinfestaron con bicloruro de mercurio al 0.25% (w/v) durante 10 minutos, se lavaron con agua destilada estéril y se establecieron en el medio de cultivo MS suplementado con 0-1 mg/L de Tidiazuron. Se obtuvieron callos nodulares con buenas características morfogénicas. Se lograron brotes en callos de seis meses de edad en la oscuridad y la regeneración de plantas en la luz. El enraizamiento de los brotes se obtuvo en el medio de cultivo MS con 1 mg/L AIB. Palabras clave: callo, cedro del Himalaya, Meliácea, reguladores del crecimiento, regeneración de plantas.text/htmlspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/489Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de BiotecnologíaRevista Colombiana de BiotecnologíaRevista Colombiana de Biotecnología; Vol. 7, núm. 2 (2005); 5-9 1909-8758 0123-3475Daquinta, Marcos and Lezcano, Yarianne and Cid, Mariela (2005) Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuron. Revista Colombiana de Biotecnología; Vol. 7, núm. 2 (2005); 5-9 1909-8758 0123-3475 .Morfogénesis in vitro de toona ciliata a partir de raquis de hojas jóvenes con tidiazuronArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTcallusHimalayan CedarMeliaceagrowth regulatorsplant regenerationcallocedro del HimalayaMeliáceareguladores del crecimientoregeneración de plantasORIGINAL489-3237-1-PB.htmtext/html42729https://repositorio.unal.edu.co/bitstream/unal/22097/1/489-3237-1-PB.htma6ee546f00b1e714f74c2755cc5a9be3MD51489-3236-1-PB.pdfapplication/pdf115086https://repositorio.unal.edu.co/bitstream/unal/22097/2/489-3236-1-PB.pdfce5fac7456a608ffd8f524e98e10a519MD52489-3239-1-PB.docapplication/msword72754https://repositorio.unal.edu.co/bitstream/unal/22097/3/489-3239-1-PB.doc4087635d2eb477cb3c0bb7aa32c8d7abMD53THUMBNAIL489-3236-1-PB.pdf.jpg489-3236-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7579https://repositorio.unal.edu.co/bitstream/unal/22097/4/489-3236-1-PB.pdf.jpg04d3f810008aeaac9218fa673e56336cMD54unal/22097oai:repositorio.unal.edu.co:unal/220972022-10-13 23:02:10.446Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |