Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+

ilustraciones, diagramas, fotografías

Autores:
Gómez Piñeros, Brayan Stiven
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2024
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86194
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86194
https://repositorio.unal.edu.co/
Palabra clave:
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
Bactris gasipaes
Iones
Bactris gasipaes
ions
Chontaduro
Fluorescencia
Quenching estático
Puntos cuánticos de carbono
Peach palm
Static quenching
Fluorescence
Carbon quantum dots
punto cuántico
quantum dot
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_def0227382c001bf78b6163457c7c46b
oai_identifier_str oai:repositorio.unal.edu.co:unal/86194
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
dc.title.translated.eng.fl_str_mv Synthesis of carbon quantum dots from peach palm peels: functionalization with biomolecules and application in Hg2+, Pb2+ and As3+ ions detection
title Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
spellingShingle Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
Bactris gasipaes
Iones
Bactris gasipaes
ions
Chontaduro
Fluorescencia
Quenching estático
Puntos cuánticos de carbono
Peach palm
Static quenching
Fluorescence
Carbon quantum dots
punto cuántico
quantum dot
title_short Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
title_full Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
title_fullStr Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
title_full_unstemmed Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
title_sort Síntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+
dc.creator.fl_str_mv Gómez Piñeros, Brayan Stiven
dc.contributor.advisor.spa.fl_str_mv Granados Oliveros, Gilma
dc.contributor.author.spa.fl_str_mv Gómez Piñeros, Brayan Stiven
dc.contributor.researchgroup.spa.fl_str_mv Nano-Inorgánica
dc.subject.ddc.spa.fl_str_mv 540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
topic 540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
Bactris gasipaes
Iones
Bactris gasipaes
ions
Chontaduro
Fluorescencia
Quenching estático
Puntos cuánticos de carbono
Peach palm
Static quenching
Fluorescence
Carbon quantum dots
punto cuántico
quantum dot
dc.subject.agrovoc.spa.fl_str_mv Bactris gasipaes
Iones
dc.subject.agrovoc.eng.fl_str_mv Bactris gasipaes
ions
dc.subject.proposal.spa.fl_str_mv Chontaduro
Fluorescencia
Quenching estático
Puntos cuánticos de carbono
dc.subject.proposal.eng.fl_str_mv Peach palm
Static quenching
Fluorescence
Carbon quantum dots
dc.subject.wikidata.spa.fl_str_mv punto cuántico
dc.subject.wikidata.eng.fl_str_mv quantum dot
description ilustraciones, diagramas, fotografías
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-05-31T18:50:30Z
dc.date.available.none.fl_str_mv 2024-05-31T18:50:30Z
dc.date.issued.none.fl_str_mv 2024
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86194
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/86194
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
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dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Granados Oliveros, Gilma81b43a9ceb44c33a7ac592ffe2168af9600Gómez Piñeros, Brayan Stiven7d36fc22201646e3aceed431765ff5b0Nano-Inorgánica2024-05-31T18:50:30Z2024-05-31T18:50:30Z2024https://repositorio.unal.edu.co/handle/unal/86194Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasEn diversos países a nivel mundial donde se practican actividades industriales, los iones mercurio (Hg2+), plomo (Pb2+) y arsénico (As3+) altamente tóxicos se liberan incontrolablemente al medio ambiente. Detectar los iones Hg2+, Pb2+ y As3+ con buena sensibilidad y selectividad es de gran importancia. En este trabajo, se reporta por primera vez la obtención de manera fácil y de bajo costo de puntos cuánticos de carbono dopados con nitrógeno (N-CQDs) a partir de cáscaras de chontaduro (Bactris gasipaes), mediante la síntesis en un solo paso asistida por microondas. Estos materiales mostraron alta solubilidad en agua y un rendimiento cuántico de fluorescencia en el visible del 20,5%. Se realizó la modificación de la superficie de los N-CQDs con L-cisteína (N-CQDs/CYS), L-histidina (N-CQDs/HIS) y L-fosfoserina (N-CQDs/FOS), mediante una reacción química (conocida como acoplamiento de amida) entre los grupos amino de los aminoácidos y los grupos carboxilo de los N-CQDs, este acoplamiento fue caracterizado mediante FT-IR y XPS. Las nanopartículas de N-CQDs/CYS, N-CQDs/HIS y N-CQDs/FOS también exhibieron alta solubilidad en agua, y se caracterizaron mediante UV-VIS y fluorescencia, obteniendo un rendimiento cuántico de fotoluminiscencia (ΦFL) del 25,4 %, 23,0%, 19,9% y una superficie con grupos tiol (de la L-cisteína), grupos imidazol (de la L-histidina) y grupos fosfato (de la L-fosfoserina) correspondientemente. Los N-CQDs/CYS, N-CQDs/HIS y N-CQDs/FOS detectaron eficientemente los iones Hg2+, Pb2+ y As3+ respectivamente (en medio acuoso) lo cual se comprobó por medio de la disminución de su fluorescencia, reportando para los N-CQDs/CYS una sensibilidad de 4,1x104 M-1 con un límite de detección (LD) de 0.20µM, para los N-CQDs/HIS una sensibilidad de 1,2x104 M-1 con un LD de 0.070µM y para los N-CQDs/FOS una sensibilidad de 3,0x104 M-1 con un LD de 0.34µM. Los N-CQDs/CYS, N-CQDs/HIS y N-CQDs/FOS evidenciaron una mayor selectividad a los iones Hg2+, Pb2+ y As3+ correspondientemente sin interferentes significativos que los afecten, excepto para los N-CQDs/HIS que tuvieron como interferentes el Cu2+ y el Cd2+. La funcionalización estratégica en la superficie de los N-CQDs con aminoácidos L-cisteína, L-histidina y L-fosfoserina, les brindó una eficiente detección, elevada sensibilidad y selectividad a los N-CQDs/CYS, N-CQDs/HIS y N-CQDs/FOS. Se realizaron mediciones de fluorescencia resuelta en el tiempo para estudiar la forma en que ocurría el apagado de la fluorescencia, cuando estaban los iones Hg2+ presentes en los N-CQDs/CYS se evidenció un mecanismo de quenching de fluorescencia estático, debido a la formación de un complejo de coordinación no fluorescente en el estado basal entre los iones Hg2+ y el grupo tiolato de los N-CQDs/CYS. Asimismo, la presencia de los iones Pb2+ en los N-CQDs/HIS genera un quenching de fluorescencia estático debido a la formación de un complejo de coordinación entre los iones Pb2+ y el grupo imidazol de los N-CQDs/HIS. Finalmente, la presencia de los iones As3+ en los N-CQDs/FOS generó también un quenching de fluorescencia estático debido a la coordinación de los iones As3+ al grupo fosfato de los N-CQDs/FOS. Los resultados indican que los nuevos materiales N-CQDs/CYS, N-CQDs/HIS y N-CQDs/FOS, al ser modificados superficialmente dirigieron la selectividad a los iones Hg2+, Pb2+ y As3+ correspondientemente, dotándolos de gran potencial para ser usados en la detección fluorescente de manera selectiva, sensible, de estos iones en medio acuoso de una forma fácil y económica. (Texto tomado de la fuente).In many countries around the world where industrial activities are practiced, highly toxic mercury (Hg2+), lead (Pb2+) and arsenic (As3+) ions are released uncontrollably into the environment. Establishing an effective strategy for the determination of Hg2+, Pb2+ and As3+ with good sensitivity and selectivity is of great importance. In this work, the easy and low-cost preparation of nitrogen-doped carbon quantum dots (N-CQDs) from peach palm peels (Bactris gasipaes) is reported for the first time by using a one-step microwave-assisted synthesis. These materials showed high water solubility and a visible fluorescence quantum yield of 20.5%. The surface of the N-CQDs was modified with L-cysteine (N-CQDs/CYS), L-histidine (N-CQDs/HIS) and L-phosphoserine (N-CQDs/FOS), using a chemical reaction (known as amide coupling) between the amino groups of the amino acids and the carboxyl groups of the N-CQDs. This coupling was characterized by FT-IR and XPS. The N-CQDs/CYS, N-CQDs/HIS and N-CQDs/FOS nanoparticles also exhibited high water solubility and were characterized by UV-VIS and fluorescence, showing a photoluminescence quantum yield (ΦFL) of 25.4%, 23.0%, 19.9% and a surface with thiol groups (from L-cysteine), imidazole groups (from L-histidine) and phosphate groups (from L-phosphoserine) respectively. The N-CQDs/CYS, N-CQDs/HIS and N-CQDs/FOS efficiently detected Hg2+, Pb2+ and As3+ ions respectively (in aqueous medium) through the decrease in their fluorescence, reporting for the N-CQDs/CYS a sensitivity of 4.1x104 M-1 with a detection limit (LD) of 0.20µM, for the N-CQDs/HIS a sensitivity of 1.2x104 M-1 with a LD of 0.070µM and for the N-CQDs/FOS a sensitivity of 3.0x104 M-1 with a LD of 0.34µM. The N-CQDs/CYS, N-CQDs/HIS and N-CQDs/FOS were correspondingly selective for Hg2+, Pb2+ and As3+ ions with no significant interfering agents, except for the N-CQDs/HIS which had Cu2+ and Cd2+ as interfering agents. The strategic functionalization of the N-CQDs surface with L-cysteine, L-histidine and L-phosphoserine amino acids provided N-CQDs/CYS, N-CQDs/HIS and N-CQDs/FOS with efficient detection, high sensitivity and selectivity. Time-resolved fluorescence measurements were performed to study the way in which fluorescence quenching occurred. When Hg2+ ions were present in the N-CQDs/CYS, a static fluorescence quenching mechanism was evidenced, due to the formation of a non-fluorescent coordination complex in the ground state between the Hg2+ ions and the thiolate group of the N-CQDs/CYS. Likewise, the presence of Pb2+ ions in the N-CQDs/HIS generates a static fluorescence quenching due to the formation of a coordination complex between the Pb2+ ions and the imidazole group of the N-CQDs/HIS. Finally, the presence of As3+ ions in the N-CQDs/FOS also generated a static fluorescence quenching due to the coordination of the As3+ ions to the phosphate group of the N-CQDs/FOS. These results indicate that the new N-CQDs/CYS, N-CQDs/HIS and N-CQDs/FOS materials which were surface modified, guided the selectivity to the Hg2+, Pb2+ and As3+ ions respectively, providing them great potential to be used in the selective and sensitive fluorescent detection of these ions in aqueous medium in an easy and economical way.DoctoradoDoctor en Ciencias - QuímicaNanomateriales con aplicación ambientalxxiv, 93 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materialesBactris gasipaesIonesBactris gasipaesionsChontaduroFluorescenciaQuenching estáticoPuntos cuánticos de carbonoPeach palmStatic quenchingFluorescenceCarbon quantum dotspunto cuánticoquantum dotSíntesis de puntos cuánticos de carbono obtenidos a partir de cáscara de chontaduro: funcionalización con biomoléculas y aplicación en la detección de iones Hg2+, Pb2+ y As3+Synthesis of carbon quantum dots from peach palm peels: functionalization with biomolecules and application in Hg2+, Pb2+ and As3+ ions detectionTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TD[1] Duffus JH. “Heavy metals” a meaningless term? 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Wiley; 2005. https://doi.org/10.1002/0470011149.EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86194/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1013650360.2024.pdf1013650360.2024.pdfTesis de Doctorado en Ciencias - Químicaapplication/pdf3777603https://repositorio.unal.edu.co/bitstream/unal/86194/2/1013650360.2024.pdfa9186e1719125a68330a8677b1c0474fMD52THUMBNAIL1013650360.2024.pdf.jpg1013650360.2024.pdf.jpgGenerated Thumbnailimage/jpeg6714https://repositorio.unal.edu.co/bitstream/unal/86194/3/1013650360.2024.pdf.jpg1767dc2cd18ee02f13dbc840a524b18aMD53unal/86194oai:repositorio.unal.edu.co:unal/861942024-08-25 23:11:14.887Repositorio Institucional Universidad Nacional de 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