Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses

ilustraciones, fotografías, diagramas

Autores:
Álvarez Alarcón, Natalie
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/85528
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/85528
https://repositorio.unal.edu.co/
Palabra clave:
540 - Química y ciencias afines::543 - Química analítica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
Cocaína
Solubilidad
Extracción
cocaine
solubility
extraction
Cocaína
Material de referencia
Estabilidad
Homogeneidad
Purificación
Diseño experimental
Cocaine
Reference material
Stability
Homogeneity
Purification
Experimental Design
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_dd3dc83e870f723ac0916f3cfe96e03c
oai_identifier_str oai:repositorio.unal.edu.co:unal/85528
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
dc.title.translated.eng.fl_str_mv Methodological development to obtain an "in-house" reference material of cocaine for forensic applications
title Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
spellingShingle Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
540 - Química y ciencias afines::543 - Química analítica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
Cocaína
Solubilidad
Extracción
cocaine
solubility
extraction
Cocaína
Material de referencia
Estabilidad
Homogeneidad
Purificación
Diseño experimental
Cocaine
Reference material
Stability
Homogeneity
Purification
Experimental Design
title_short Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
title_full Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
title_fullStr Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
title_full_unstemmed Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
title_sort Desarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forenses
dc.creator.fl_str_mv Álvarez Alarcón, Natalie
dc.contributor.advisor.none.fl_str_mv Garzón Méndez, William Fernando
Melo Martínez, Sandra Esperanza
dc.contributor.author.none.fl_str_mv Álvarez Alarcón, Natalie
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Investigación en Química de Alimentos
dc.contributor.orcid.spa.fl_str_mv Álvarez Alarcón, Natalie [0000000278479918]
dc.contributor.researchgate.spa.fl_str_mv Álvarez Alarcón, Natalie [Natalie-Alvarez-Alarcon]
dc.subject.ddc.spa.fl_str_mv 540 - Química y ciencias afines::543 - Química analítica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
topic 540 - Química y ciencias afines::543 - Química analítica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
Cocaína
Solubilidad
Extracción
cocaine
solubility
extraction
Cocaína
Material de referencia
Estabilidad
Homogeneidad
Purificación
Diseño experimental
Cocaine
Reference material
Stability
Homogeneity
Purification
Experimental Design
dc.subject.agrovoc.spa.fl_str_mv Cocaína
Solubilidad
Extracción
dc.subject.agrovoc.eng.fl_str_mv cocaine
solubility
extraction
dc.subject.proposal.spa.fl_str_mv Cocaína
Material de referencia
Estabilidad
Homogeneidad
Purificación
Diseño experimental
dc.subject.proposal.eng.fl_str_mv Cocaine
Reference material
Stability
Homogeneity
Purification
Experimental Design
description ilustraciones, fotografías, diagramas
publishDate 2023
dc.date.issued.none.fl_str_mv 2023-08-01
dc.date.accessioned.none.fl_str_mv 2024-01-30T16:46:47Z
dc.date.available.none.fl_str_mv 2024-01-30T16:46:47Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/85528
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/85528
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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Aguilar, Y.; Ahumada, D.; Barrios, J.; González, I.; Morales, L.; Salinas, A.; Torres, G. Desarrollo de Materiales de Referencia In-House Para El Control de Calidad de Residuos de Plaguicidas En Alimentos; 2023.
Wood, S.; Botha, A. The New ISO Guide 80: Guidance for the in-House Preparation of Quality Control Materials (QCMs). Accredit. Qual. Assur. 2014, 19 (6), 477–480. https://doi.org/10.1007/s00769-014-1084-1.
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Gupta, R.; Shim, J.-J. Solubility in Supercritical Carbon Dioxide; 2007.
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Ballesteros Vivas, D. Estudio Del Potencial Antiproliferativo de Extractos Obtenidos de Residuos Frutícolas Desde Las Perspectivas de La Química Verde y La Alimentómica, Universidad Nacional de Colombia, 2020.
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dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Garzón Méndez, William Fernandoadf2e2c34907256992b5fa0851aa6641Melo Martínez, Sandra Esperanzab9210344d4e28b98af8896456c41bc0bÁlvarez Alarcón, Natalie2671f311b1e47fb500aa4ba4415a3ecdGrupo de Investigación en Química de AlimentosÁlvarez Alarcón, Natalie [0000000278479918]Álvarez Alarcón, Natalie [Natalie-Alvarez-Alarcon]2024-01-30T16:46:47Z2024-01-30T16:46:47Z2023-08-01https://repositorio.unal.edu.co/handle/unal/85528Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, diagramasEn este trabajo se desarrolló una metodología para obtener un material de referencia “in-house” (RMih) de cocaína a partir de una muestra incautada, teniendo en cuenta diferentes lineamientos internacionales, así como los principios de la Química Verde y los Objetivos de Desarrollo Sostenible (ODS). Para llevar a cabo esto, se evaluaron diferentes técnicas de extracción y purificación, tales como (i) cromatografía en columna (CC) y (ii) una estrategia secuencial de extracción con fluidos supercríticos (SFE) y extracción con líquidos presurizados (PLE). Asimismo, se emplearon los parámetros de solubilidad de Hansen (HSP), para seleccionar el mejor disolvente verde a emplear en PLE. Por otro lado, se caracterizaron el material de partida y los materiales obtenidos en las diferentes técnicas de extracción y purificación usadas, por medio de cromatografía de gases acoplada a espectrometría de masas (GC-MS) y cromatografía de gases con detector de ionización de llama (GC-FID). Teniendo en cuenta lo anterior, se realizó la evaluación de dos diseños experimentales para determinar la influencia de los factores empleados en PLE, en torno a la variable respuesta correspondiente a la pureza del analito. La muestra incautada presentó un valor de pureza del 81.3 %. Por CC se logró obtener el analito con una pureza del 96.4 %, en donde se empleó una fase móvil correspondiente a cloroformo-metanol (6:1). Por otro lado, empleando la estimación de los HSP para la cocaína, se determinó que el disolvente más adecuado para emplear en PLE era el acetato de etilo (EtOAc). La estrategia secuencial ambientalmente amigable SFE-PLE (empleando como disolventes CO2 supercrítico y EtOAc, respectivamente) permitió obtener el analito con una pureza del 86.1 %. Finalmente, se propuso un diseño metodológico con el objetivo de evaluar la homogeneidad y la estabilidad del candidato a RMih. (Texto tomado de la fuente)In this work, a methodology was developed to obtain an “in-house” reference material (RMih) of cocaine from a seized sample, considering different international guidelines, as well as the principles of Green Chemistry and the Sustainable Development Goals (SDG). To carry out this, different extraction and purification techniques were evaluated, such as (i) column chromatography (CC) and (ii) a sequential strategy of supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE). Likewise, the Hansen solubility parameters (HSP) were used to select the best green solvent in PLE. On the other hand, the starting material and the materials obtained in the different extraction and purification techniques used were characterized using gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with a detector flame ionization (GC-FID). Considering the above, the evaluation of two experimental designs was carried out to determine the influence of the factors used in PLE, around the response variable corresponding to the purity of the analyte. The seized sample had a purity value of 81.3 %. By CC, the analyte was obtained with a purity of 96.4 %, where a mobile phase corresponding to chloroform-methanol (6:1) was used. On the other hand, using the estimation of HSP for cocaine, it was determined that the most suitable solvent to use in PLE was ethyl acetate (EtOAc). The environmentally friendly sequential strategy SFE-PLE (using supercritical CO2 and EtOAc as solvents, respectively) allowed obtaining the analyte with a purity of 86.1 %. Finally, a methodological design was proposed with the objective of evaluating the homogeneity and stability of the RMih candidate.MaestríaMagíster en Ciencias - Químicaxx, 73 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::543 - Química analítica540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materialesCocaínaSolubilidadExtraccióncocainesolubilityextractionCocaínaMaterial de referenciaEstabilidadHomogeneidadPurificaciónDiseño experimentalCocaineReference materialStabilityHomogeneityPurificationExperimental DesignDesarrollo metodológico para obtener un material de referencia “in-house” de cocaína para aplicaciones forensesMethodological development to obtain an "in-house" reference material of cocaine for forensic applicationsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMOficina de las Naciones Unidas contra la Droga y el Delito (UNODC). 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Estudio Del Potencial Antiproliferativo de Extractos Obtenidos de Residuos Frutícolas Desde Las Perspectivas de La Química Verde y La Alimentómica, Universidad Nacional de Colombia, 2020.Mohammad, A.; Inamuddin; El-Desoky, G. Green Solvents I: Properties and Applications in Chemistry; 2012. https://doi.org/10.1007/978-94-007-1712-1.EstudiantesInvestigadoresMaestrosPúblico generalORIGINAL1032458311.2023.pdf1032458311.2023.pdfTesis de Maestría en Químicaapplication/pdf1788333https://repositorio.unal.edu.co/bitstream/unal/85528/2/1032458311.2023.pdfb4d418e9479c0b56c4d906aa881996e0MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85528/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL1032458311.2023.pdf.jpg1032458311.2023.pdf.jpgGenerated Thumbnailimage/jpeg5157https://repositorio.unal.edu.co/bitstream/unal/85528/3/1032458311.2023.pdf.jpg0205586e542890ab17c19e78696cfcb2MD53unal/85528oai:repositorio.unal.edu.co:unal/855282024-01-30 23:03:50.805Repositorio Institucional Universidad Nacional de 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