Production and purification of igy antibodies as a novel tool to purify the nr1 subunit of nmda recepto
Producing polyclonal antibodies (IgY) inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit). We produced specific antibodiesagainst expression products b...
- Autores:
-
Reyes Montaño, Edgar Antonio
Lareo, Leonardo
Pérez, Gerardo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/41066
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/41066
http://bdigital.unal.edu.co/31163/
- Palabra clave:
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| Summary: | Producing polyclonal antibodies (IgY) inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit). We produced specific antibodiesagainst expression products by thealternative splicing of the gene encodingNMDA receptor NR1 subunit in adult ratbrain. Three peptides corresponding tothe splicing sites (N1, C1 and C2’ cassettes)were designed, synthesised and usedindividually as antigens in hens. Specificimmunoglobulins were purified fromyolks. The antibodies were then used forpurifying the NMDA receptor NR1 subunitusing affinity chromatography couplingthe three antibodies to the support.R |
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