Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado
ilustraciones, diagramas
- Autores:
-
Zapata Carmona, Kelly Vanessa
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/83312
- Palabra clave:
- 590 - Animales::599 - Mamíferos
Conservación del semen
Semen conservation
Calidad seminal
Estado redox
Flavonolignanos
Refrigeración
Silimarina
Semen porcino
Flavonolignans
Porcine semen
Redox status
Refrigeration
Seminal quality
Silymarin
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| dc.title.translated.eng.fl_str_mv |
Antioxidant activity of silymarin and its components (silydianin, silychristin and silybinin) and their effect on the preservation of refrigerated boar semen (Sus scrofa) |
| title |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| spellingShingle |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado 590 - Animales::599 - Mamíferos Conservación del semen Semen conservation Calidad seminal Estado redox Flavonolignanos Refrigeración Silimarina Semen porcino Flavonolignans Porcine semen Redox status Refrigeration Seminal quality Silymarin |
| title_short |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| title_full |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| title_fullStr |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| title_full_unstemmed |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| title_sort |
Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
| dc.creator.fl_str_mv |
Zapata Carmona, Kelly Vanessa |
| dc.contributor.advisor.none.fl_str_mv |
Restrepo Betancur, Giovanni |
| dc.contributor.author.none.fl_str_mv |
Zapata Carmona, Kelly Vanessa |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Biotecnología Animal (Giba) |
| dc.contributor.orcid.spa.fl_str_mv |
Zapata Carmona, Kelly Vanessa [0000-0002-3559-5783] |
| dc.subject.ddc.spa.fl_str_mv |
590 - Animales::599 - Mamíferos |
| topic |
590 - Animales::599 - Mamíferos Conservación del semen Semen conservation Calidad seminal Estado redox Flavonolignanos Refrigeración Silimarina Semen porcino Flavonolignans Porcine semen Redox status Refrigeration Seminal quality Silymarin |
| dc.subject.lemb.spa.fl_str_mv |
Conservación del semen |
| dc.subject.lemb.eng.fl_str_mv |
Semen conservation |
| dc.subject.proposal.spa.fl_str_mv |
Calidad seminal Estado redox Flavonolignanos Refrigeración Silimarina Semen porcino |
| dc.subject.proposal.eng.fl_str_mv |
Flavonolignans Porcine semen Redox status Refrigeration Seminal quality Silymarin |
| description |
ilustraciones, diagramas |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022-11 |
| dc.date.accessioned.none.fl_str_mv |
2023-02-06T15:23:28Z |
| dc.date.available.none.fl_str_mv |
2023-02-06T15:23:28Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/83312 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/83312 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
| language |
spa |
| dc.relation.indexed.spa.fl_str_mv |
RedCol LaReferencia Agrovoc |
| dc.relation.references.spa.fl_str_mv |
Abouzid, S., & Ahmed, O. M. (2013). Silymarin flavonolignans: Structure-activity relationship and biosynthesis. Studies in Natural Products Chemistry (Vol. 40, pp. 469–484). https://doi.org/10.1016/B978-0-444-59603-1.00014-X Aghashahi, M., Momeni, H. R., & Darbandi, N. (2020). Impact of aluminium toxicity on vital human sperm parameters—Protective effects of silymarin. Andrologia, 1–10. https://doi.org/10.1111/and.13742 Ahmed, H., Amin, H., Clement, A. (2022). Silymarin abrogates acrylamide-induced oxidative stressmediated testicular toxicity via modulation of antioxidant mechanism, DNA damage, endocrine deficit and sperm quality in rats. Andrologia, 54(9). https://doi.org/10.1111/and.14491 Aitken, R. J., Gibb, Z., Baker, M. A., Drevet, J., & Gharagozloo, P. (2016). Causes and consequences of oxidative stress in Spermatozoa. Reproduction, Fertility and Development, 28(1–2), 1–10. https://doi.org/10.1071/RD15325 Arts, M., Dallinga, J., Voss, H., Haenen, G., & Bast, A. (2004). A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chemistry, 88(4), 567–570. https://doi.org/10.1016/J.FOODCHEM.2004.02.008 Awda, B. J., Mackenzie-Bell, M., & Buhr, M. M. (2009). Reactive oxygen species and boar sperm function. Biology of Reproduction, 81(3), 553–561. https://doi.org/10.1095/biolreprod.109.076471 Baeeri, M., Mohammadi-Nejad, S., Rahimifard, M., Navaei-Nigjeh, M., Moeini-Nodeh, S., Khorasani, R., & Abdollahi, M. (2018). Molecular and biochemical evidence on the protective role of ellagic acid and silybin against oxidative stress-induced cellular aging. Molecular and Cellular Biochemistry, 441(1–2), 21–33. https://doi.org/10.1007/s11010-017-3172-0 Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/ABIO.1996.0292 Biedermann, D., Buchta, M., Holečková, V., Sedlák, D., Valentová, K., Cvačka, J., Bednárová, L., Křenková, A., Kuzma, M., Škuta, C., Peikerová, Ž., Bartůněk, P., & Křen, V. (2016). Silychristin: Skeletal Alterations and Biological Activities. Journal of Natural Products, 79(12), 3086–3092. https://doi.org/10.1021/acs.jnatprod.6b00750 Biedermann, D., Moravcová, V., Valentová, K., Kuzma, M., Petrásková, L., Císařová, I., & Křen, V. (2019). Oxidation of flavonolignan silydianin to unexpected lactone-acid derivative. Phytochemistry Letters, 14–20. https://doi.org/10.1016/j.phytol.2019.01.006 Bollwein, H., & Bittner, L. (2018). Impacts of oxidative stress on bovine sperm function and subsequent in vitro embryo development. Animal Reproduction, 15, 703–710. https://doi.org/10.21451/1984- 3143-AR2018-0041 Cordova, A., Ruiz, C., Córdova, C., Córdova, M., Eulogio, J., Guerra, J., Rodríguez, B., & Salinas, K. (2009). Estrés oxidativo y antioxidantes en la conservación espermática. Revista Computense de Ciencias Veterinarias, 3(1), 1–38. El-Sheshtawy, R., & El-Nattat, W. (2017). Impact of silymarin enriched semen extender on bull sperm preservability. Asian Pacific Journal of Reproduction, 6(2), 81–84. https://doi.org/10.12980/apjr.6.20170206 Eskandari, F., & Momeni, H. R. (2016). Protective effect of silymarin on viability, motility and mitochondrial membrane potential of ram sperm treated with sodium arsenite. International Journal of Reproductive BioMedicine, 14(6), 397–402. https://doi.org/10.29252/ijrm.14.6.397 Etemadi, T., Reza, H., & Asghar, A. (2020). Impact of silymarin on cadmium-induced apoptosis in human spermatozoa. Andrologia, 52(11), 1-9. https://doi.org/10.1111/and.13795. Etemadi, T., Reza, H., Darbandi, N., & Hussein, M. (2022). Silymarin modulates cadmium-induced oxidative stress in human spermatozoa. Andrologia, 52(11), 1-9. https://doi.org/ 10.1111/and.14475 Fair, S., & Romero-Aguirregomezcorta, J. (2019). Implications of boar sperm kinematics and rheotaxis for fertility after preservation. Theriogenology, 137, 15–22. https://doi.org/10.1016/J.THERIOGENOLOGY.2019.05.032 Faudale, M., Viladomat, F., Bastida, J., Poli, F., & Codina, C. (2008). Antioxidant activity and phenolic composition of wild, edible, and medicinal fennel from different Mediterranean countries. Journal of Agricultural and Food Chemistry, 56(6), 1912–1920. https://doi.org/10.1021/jf073083c Feng, T. Y., Lv, D. L., Zhang, X., Du, Y. Q., Yuan, Y. T., Chen, M. J., Xi, H. M., Li, Y., Han, N., & Hu, J. H. (2020). Rosmarinic acid improves boar sperm quality, antioxidant capacity and energy metabolism at 17°C via AMPK activation. Reproduction in Domestic Animals, 55(12), 1714–1724. https://doi.org/10.1111/rda.13828 Funahashi, H., & Sano, T. (2005). Select antioxidants improve the function of extended boar semen stored at 10°C. Theriogenology, 63(6), 1605–1616. https://doi.org/10.1016/j.theriogenology.2004.06.016 Gillan, L., Evans, G., & Maxwell, W. M. C. (2005). Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology, 63(2), 445–457. https://doi.org/10.1016/j.theriogenology.2004.09.024 Gülçin, İ. (2010). Antioxidant properties of resveratrol: A structure-activity insight. Innovative Food Science and Emerging Technologies, 11, 210–218. https://doi.org/10.1016/j.ifset.2009.07.002 Gulcin, İ. (2020). Antioxidants and antioxidant methods: an updated overview. Archives of Toxicology, 94, 651–715. https://doi.org/10.1007/s00204-020-02689-3 Guo, H., Gong, Y., He, B., & Zhao, R. (2017). Relationships between mitochondrial DNA content, mitochondrial activity, and boar sperm motility. Theriogenology, 87, 276–283. https://doi.org/10.1016/j.theriogenology.2016.09.005 Guthrie, H. D., & Welch, G. R. (2006). Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescenceactivated flow cytometry. Journal of Animal Science, 84(8), 2089–2100. https://doi.org/10.2527/JAS.2005-766 Haddad, P. S., Haddad, Y., Vallerand, D., & Brault, A. (2011). Antioxidant and Hepatoprotective Effects of Silibinin in a Rat Model of Nonalcoholic Steatohepatitis. Evidence-Based Complementary and Alternative Medicine : ECAM, 2011, 1–10. https://doi.org/10.1093/ECAM/NEP164 Hidalgo, D. M., Barón, F. J., Bragado, M. J., Carmona, P., Robina, A., García-Marín, L. J., & Gil, M. C. (2011). The effect of melatonin on the quality of extended boar semen after long-term storage at 17 °C. Theriogenology, 75(8), 1550–1560. https://doi.org/10.1016/j.theriogenology.2010.12.021 Hidalgo, D. M. (2013). Fisiología celular y calidad seminal durante la conservación del semen porcino refrigerado. In Fisiología celular y calidad seminal durante la conservación del semen porcino refrigerado. [Universidad de Extremadura]. https://dehesa.unex.es:8443/handle/10662/799?mode=full Jang, H.-Y., Kong, H. S., Choi, B.-Y., Shin, J.-S., Cheong, H.-T., Kim, J.-T., Park, I.-C., Park, C.-K., & Yang, B.- K. (2011). Protective Effects of Silymarin against the Toxicity of Bisphenol A (BPA) on Boar Sperm Quality. Journal of Embryo Transfer, 26(4), 257–263. Juarez, J. D. (2009). Efecto de la velocidad de enfriamiento en la congelabilidad de espermatozoides porcinos. [Universidad de Murcia]. https://riunet.upv.es:443/handle/10251/14316 Khoi, H. X., Shimizu, K., Yoneda, Y., Minagawa, I., Abe, Y., Kuwabara, Y., Sasanami, T., & Kohsaka, T. (2021). Monitoring the reactive oxygen species in spermatozoa during liquid storage of boar semen and its correlation with sperm motility, free thiol content and seasonality. Andrologia, 53(11), e14237. https://doi.org/10.1111/AND.14237 Kumaresan, A., Kadirvel, G., Bujarbaruah, K. M., Bardoloi, R. K., Das, A., Kumar, S., & Naskar, S. (2009). Preservation of boar semen at 18°C induces lipid peroxidation and apoptosis like changes in spermatozoa. Animal Reproduction Science, 110, 162–171. https://doi.org/10.1016/j.anireprosci.2008.01.006 Luberda, Z. (2005). The role of glutathione in mammalian gametes. Reproductive Biology, 5(1), 5–17. Martín-Hidalgo, D., Hurtado de Llera, A., Henning, H., Wallner, U., Waberski, D., Bragado, M. J., Gil, M. C., & García-Marín, L. J. (2013). The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17oC. Journal of Agricultural Science, 5(8), 231–242. https://doi.org/10.5539/jas.v5n8p231 Martínez, F., Mata, M., Álvarez, M., Álvarez, M., Anel, L., & de Paz, P. (2010). Probes and techniques for sperm evaluation by flow cytometry. Reproduction in Domestic Animals, 45(SUPPL. 2), 67–78. https://doi.org/10.1111/j.1439-0531.2010.01622.x Merino, O., Figueroa, E., Cheuquemán, C., Valdebenito, I., Isachenko, V., Isachenko, E., Sánchez, R., Farías, J., & Risopatrón, J. (2017). Short-term storage of salmonids semen in a sodium alginatebased extender. Andrologia, 49(5), 1–5. https://doi.org/10.1111/and.12661 Munteanu, I. G., & Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. International Journal of Molecular Sciences, 22(7). https://doi.org/10.3390/IJMS22073380 Oufi, H. G., & Al-Shawi, N. N. (2014). The effects of different doses of silibinin in combination with methotrexate on testicular tissue of mice. European Journal of Pharmacology, 730(1), 36–40. https://doi.org/10.1016/J.EJPHAR.2014.02.010 Pascual, C., Gonz, R., Armesto, J., & Muriel, P. (1993). Effect of silymarin and silybinin on oxygen radicals. Drug Development Research, 29(1), 73–77. https://doi.org/10.1002/ddr.430290109 Pereira, B. A., Rocha, L. G. P., Teles, M. C., Silva, W. E., Barbosa, J. A., Rabelo, S. S., Uchoa, A. S., RodriguezGil, J. E., Pereira, L. J., & Zangeronimo, M. G. (2019). Addition of chlorogenic acid and caffeine during the processing of cooled boar semen. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 71(2), 489–499. https://doi.org/10.1590/1678-4162-10415 Pezo, F., Romero, F., Zambrano, F., & Sánchez, R. S. (2019). Preservation of boar semen: An update. Reproduction in Domestic Animals, 54(3), 423–434. https://doi.org/10.1111/rda.13389 Potra, G., Babes, P., Calniceanu, H., Popa, A., Ciavoi, G., Iova, G., Ganea, M., & Scrobotă, I. (2021). AntiInflammatory and Antioxidant Properties of Carvacrol and Magnolol, in Periodontal Disease and Diabetes Mellitus. Molecules, 26(22), 6899. https://doi.org/10.3390/molecules26226899 Pourheydar, B., Azarm, F., Farjah, G., Karimipour, M., & Pourheydar, M. (2021). Effect of silymarin and metformin on the sperm parameters and histopathological changes of testes in diabetic rats: An experimental study. International Journal of Reproductive BioMedicine, 19(12), 1091–1104. https://doi.org/10.18502/ijrm.v19i12.10060 Pyszková, M., Biler, M., Biedermann, D., Valentová, K., Kuzma, M., Vrba, J., Ulrichová, J., Sokolová, R., Mojović, M., Popović-Bijelić, A., Kubala, M., Trouillas, P., Křen, V., & Vacek, J. (2016). Flavonolignan 2,3-dehydroderivatives: Preparation, antiradical and cytoprotective activity. Free Radical Biology and Medicine, 90, 114–125. https://doi.org/10.1016/j.freeradbiomed.2015.11.014 Rajnochová, A., Gabrielová, · Eva, Ulrichová, J., Zálešák, B., Biedermann, · David, & Vostálová, J. (2019). A pilot study of the UVA-photoprotective potential of dehydrosilybin, isosilybin, silychristin, and silydianin on human dermal fibroblasts. Archives of Dermatological Research, 311, 477–490. https://doi.org/10.1007/s00403-019-01928-7 Reina, M., & Martínez, A. (2016). Is Silybin the Best Free Radical Scavenger Compound in Silymarin? Journal of Physical Chemistry B, 120(20), 4568–4578. https://doi.org/10.1021/acs.jpcb.6b02807 Roostaei-Ali Mehr, M., & Parisoush, P. (2016). Effect of different levels of silymarin and caproic acid on storage of ram semen in liquid form. Reproduction in Domestic Animals, 51(4), 569–574. https://doi.org/10.1111/rda.12721 Surai, P. F. (2015). Silymarin as a natural antioxidant: An overview of the current evidence and perspectives. Antioxidants, 4(1), 204–247. https://doi.org/10.3390/antiox4010204 Taleb, A., Ahmad, K. A., Ihsan, A. U., Qu, J., Lin, N., Hezam, K., Koju, N., Hui, L., & Qilong, D. (2018). Antioxidant effects and mechanism of silymarin in oxidative stress induced cardiovascular diseases. Biomedicine and Pharmacotherapy, 102(January), 689–698. https://doi.org/10.1016/j.biopha.2018.03.140 Temamogullari, F., Atessahin, A., Cebi Sen, C., Yumusak, N., & Dogru, M. (2021). Protective role of silibinin over nickel sulfate-induced reproductive toxicity in male rats. Polish Journal of Veterinary Sciences, 24(1), 29–34. https://doi.org/10.24425/pjvs.2020.135817 Teixeira, S. M. P., Chaveiro, A., & Moreira da Silva, F. (2015). Effect of Conjugated Linoleic Acid on Boar Semen Quality After Long-term Refrigeration at 17°C. Reproduction in Domestic Animals, 50(4), 604–610. https://doi.org/10.1111/rda.12535 Tian, X., Li, D., He, Y., Zhang, W., He, H., Du, R., Pang, W., Yang, G., & Yu, T. (2019). Supplementation of salvianic acid A to boar semen extender to improve seminal quality and antioxidant capacity. Animal Science Journal, 90(9), 1142–1148. https://doi.org/10.1111/ASJ.13263 Torres, P., Fischman, M. L., Acerbo, M., García, C., Míguez, M., Domínguez, J., & Cisale, H. (2014). Análisis de diluyentes comerciales de semen porcino refrigerado durante 4 días: resultados preliminares. Archivos de Zootecnia, 63(243), 547–550. https://doi.org/10.4321/s0004-05922014000300015 Wellington, K., & Jarvis, B. (2001). Silymarin: A review of its clinical properties in the management of hepatic disorders Zielińska-Przyjemska, M., & Wiktorowicz, K. (2006). An In vitro Study of the Protective Effect of the Flavonoid Silydianin against Reactive Oxygen Species. Phytother. Res, 20, 115–119. https://doi.org/10.1002/ptr.1812 |
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Medellín - Ciencias - Maestría en Ciencias - Biotecnología |
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Facultad de Ciencias |
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Medellín, Colombia |
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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Restrepo Betancur, Giovannibd03434c72051e913ab3e03d3c37d88dZapata Carmona, Kelly Vanessa01a3ae91a7558cd690cacbc85cd855af600Grupo de Investigación en Biotecnología Animal (Giba)Zapata Carmona, Kelly Vanessa [0000-0002-3559-5783]2023-02-06T15:23:28Z2023-02-06T15:23:28Z2022-11https://repositorio.unal.edu.co/handle/unal/83312Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasDurante la refrigeración, el espermatozoide porcino puede experimentar estrés oxidativo, osmótico, químico y térmico, que afectan su capacidad fecundante. La adición de antioxidantes al diluyente espermático constituye una alternativa para mitigar dichas alteraciones. El objetivo de esta investigación fue evaluar la capacidad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) sometido a refrigeración. Para ello, quince eyaculados de cinco cerdos fueron diluidos en MRA, suplementados con Silimarina, Silibinina, Silicristina y Silidianina y refrigerados cinco días a 16°C. Cada 24 h se evaluó la movilidad y cinética espermática con un CASA IVOS. A las 0 y 96 h se determinaron la funcionalidad de membrana por test hipoosmótico; la producción de EROs y la capacidad antioxidante total (ABTS-FRAP), por espectrofluorimetría; el potencial de membrana mitocondrial, la integridad estructural y la peroxidación lipídica mediante citometría de flujo, con JC-1, SYBR14/IP y Bodipy respectivamente. Se realizaron comparaciones de medias por Duncan, se calculó un índice para calidad espermática y se hicieron análisis de regresión para movilidad y cinética espermática, y para cinética de EROs. La suplementación con Silidianina (10-20 µM) incrementó MT, MP, Δ¥M y VE, y redujo la producción de EROs. Silicristina, a partir de 20 µM, disminuyó la MT, MP, Δ¥M y VE. Mientras que Silimarina redujo la producción de EROs/minuto. En definitiva, la Silimarina y sus componentes pueden incrementar la calidad espermática, reducir la generación de EROs, potenciar la CAT y modificar el Δ¥M en el semen porcino refrigerado. (Texto tomado de la fuente)In refrigerated conditions, boar spermatozoa are susceptible to oxidative, osmotic, chemical and thermal stress, affecting their fertilizing capacity. The addition of antioxidants to the sperm extender is an alternative to mitigate these alterations. The objective of this research was to evaluate the antioxidant capacity of Silymarin and its components (Silidianin, Silicristin and Silibinin) and their effect on the preservation of refrigerated boar semen (Sus scrofa). For this purpose, fifteen ejaculates from five swine were diluted in MRA, supplemented with Silymarin, Silybinin, Silychristin and Silydianin and refrigerated for five days at 16°C. Sperm motility and sperm kinetics were evaluated every 24 h with a CASA IVOS. At 0 and 96 h, membrane functionality was determined by hypoosmotic test; EROs production and total antioxidant capacity (ABTS-FRAP), by spectrofluorimetry; mitochondrial membrane potential, structural integrity and lipid peroxidation by flow cytometry with JC-1, SYBR14/PI and Bodipy probes, respectively. Duncan mean comparisons were performed, an index for sperm quality was calculated and regression analyses were performed for sperm motility and sperm kinetics, and for EROs kinetics. Silydianin supplementation (10-20 µM) increased MT, MP, Δ¥M and VE, and reduced EROs production. Silychristin, from 20 µM, decreased MT, MP, Δ¥M and VE. While Silymarin reduced EROs/minute production. In conclusion, Silymarin and its components can improve sperm quality, reduce EROs generation, enhance CAT and modify Δ¥M in refrigerated boar semen.MaestríaMagíster en Ciencias - BiotecnologíaReproducción AnimalÁrea Curricular Biotecnologíaxvi, 107 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín590 - Animales::599 - MamíferosConservación del semenSemen conservationCalidad seminalEstado redoxFlavonolignanosRefrigeraciónSilimarinaSemen porcinoFlavonolignansPorcine semenRedox statusRefrigerationSeminal qualitySilymarinActividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigeradoAntioxidant activity of silymarin and its components (silydianin, silychristin and silybinin) and their effect on the preservation of refrigerated boar semen (Sus scrofa)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferenciaAgrovocAbouzid, S., & Ahmed, O. 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Res, 20, 115–119. https://doi.org/10.1002/ptr.1812EstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83312/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1152696683.2022.pdf1152696683.2022.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf3099794https://repositorio.unal.edu.co/bitstream/unal/83312/3/1152696683.2022.pdff4a55778fdaaf26d9fc758bd8cf40042MD53THUMBNAIL1152696683.2022.pdf.jpg1152696683.2022.pdf.jpgGenerated Thumbnailimage/jpeg5656https://repositorio.unal.edu.co/bitstream/unal/83312/4/1152696683.2022.pdf.jpgc84f318219b66f173ddfa75aa67e3a4fMD54unal/83312oai:repositorio.unal.edu.co:unal/833122023-08-15 23:04:26.81Repositorio Institucional Universidad Nacional de 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