Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles
ilustraciones, fotografías, gráficas
- Autores:
-
Mantilla Esparza, Fabián Andrés
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/81116
- Palabra clave:
- 570 - Biología::571 - Fisiología y temas relacionados
Enzymes
Purkinje Cells
Enzimas
Células de Purkinje
Neurons
Neuronas
Litio
Célula de Purkinje
Trastorno bipolar
Embrión de pollo
Proteína Gq
Cultivo celular primario
Fosfolipasa C
Lithium
Purkinje cell
Bipolar disorder
Chick embryo
Gq protein
Primary cell culture
Phospholipase C
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| dc.title.translated.eng.fl_str_mv |
Implementation of a primary neuronal culture as a model for the study of modulatory mechanisms on the phosphoinositide signaling pathway |
| title |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| spellingShingle |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles 570 - Biología::571 - Fisiología y temas relacionados Enzymes Purkinje Cells Enzimas Células de Purkinje Neurons Neuronas Litio Célula de Purkinje Trastorno bipolar Embrión de pollo Proteína Gq Cultivo celular primario Fosfolipasa C Lithium Purkinje cell Bipolar disorder Chick embryo Gq protein Primary cell culture Phospholipase C |
| title_short |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| title_full |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| title_fullStr |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| title_full_unstemmed |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| title_sort |
Implementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositoles |
| dc.creator.fl_str_mv |
Mantilla Esparza, Fabián Andrés |
| dc.contributor.advisor.spa.fl_str_mv |
Gómez Correa, María del Pilar Nasi Lignarolo, Enrico |
| dc.contributor.author.spa.fl_str_mv |
Mantilla Esparza, Fabián Andrés |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biofísica de la Señalización Celular |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::571 - Fisiología y temas relacionados |
| topic |
570 - Biología::571 - Fisiología y temas relacionados Enzymes Purkinje Cells Enzimas Células de Purkinje Neurons Neuronas Litio Célula de Purkinje Trastorno bipolar Embrión de pollo Proteína Gq Cultivo celular primario Fosfolipasa C Lithium Purkinje cell Bipolar disorder Chick embryo Gq protein Primary cell culture Phospholipase C |
| dc.subject.decs.eng.fl_str_mv |
Enzymes Purkinje Cells |
| dc.subject.decs.spa.fl_str_mv |
Enzimas Células de Purkinje |
| dc.subject.lemb.eng.fl_str_mv |
Neurons |
| dc.subject.lemb.spa.fl_str_mv |
Neuronas |
| dc.subject.proposal.spa.fl_str_mv |
Litio Célula de Purkinje Trastorno bipolar Embrión de pollo Proteína Gq Cultivo celular primario Fosfolipasa C |
| dc.subject.proposal.eng.fl_str_mv |
Lithium Purkinje cell Bipolar disorder Chick embryo Gq protein Primary cell culture Phospholipase C |
| description |
ilustraciones, fotografías, gráficas |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-02T20:09:50Z |
| dc.date.available.none.fl_str_mv |
2022-03-02T20:09:50Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/81116 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/81116 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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(2009). Mechanisms of metabotropic glutamate receptor-mediated synaptic signalling in cerebellar Purkinje cells. Acta Physiologica, 195(1), 79-90. https://doi.org/10.1111/j.1748-1716.2008.01923.x Heintz, T. G., Eva, R., & Fawcett, J. W. (2016). Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. PLOS ONE, 11(8), e0158558. https://doi.org/10.1371/journal.pone.0158558 Hibar, D. P., Westlye, L. T., Doan, N. T., Jahanshad, N., Cheung, J. W., Ching, C. R. K., Versace, A., Bilderbeck, A. C., Uhlmann, A., Mwangi, B., Krämer, B., Overs, B., Hartberg, C. B., Abé, C., Dima, D., Grotegerd, D., Sprooten, E., Bøen, E., Jimenez, E., … Andreassen, O. A. (2018). Cortical abnormalities in bipolar disorder: An MRI analysis of 6503 individuals from the ENIGMA Bipolar Disorder Working Group. Molecular Psychiatry, 23(4), 932-942. https://doi.org/10.1038/mp.2017.73 Hibar, D. P., Westlye, L. T., van Erp, T. G. M., Rasmussen, J., Leonardo, C. D., Faskowitz, J., Haukvik, U. K., Hartberg, C. B., Doan, N. T., Agartz, I., Dale, A. M., Gruber, O., Krämer, B., Trost, S., Liberg, B., Abé, C., Ekman, C. J., Ingvar, M., Landén, M., … Andreassen, O. A. (2016). Subcortical volumetric abnormalities in bipolar disorder. Molecular Psychiatry, 21(12), 1710-1716. https://doi.org/10.1038/mp.2015.227 Hilgemann, D. W. (2007). On the physiological roles of PIP2 at cardiac Na+–Ca2+ exchangers and KATP channels: A long journey from membrane biophysics into cell biology. The Journal of Physiology, 582(Pt 3), 903-909. https://doi.org/10.1113/jphysiol.2007.132746 Hinoi, E., Ogita, K., Takeuchi, Y., Ohashi, H., Maruyama, T., & Yoneda, Y. (2001). Characterization with [3H]quisqualate of group I metabotropic glutamate receptor subtype in rat central and peripheral excitable tissues. Neurochemistry International, 38(3), 277-285. https://doi.org/10.1016/s0197-0186(00)00075-9 Hirano, T. (2018). 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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gómez Correa, María del Pilar69446e21238a4cfcfe3166184ed45e6bNasi Lignarolo, Enricob880efc08f05d0579d6f076cca5dd9cdMantilla Esparza, Fabián Andréscdb22f90cad797181544670057e3c56aBiofísica de la Señalización Celular2022-03-02T20:09:50Z2022-03-02T20:09:50Z2021https://repositorio.unal.edu.co/handle/unal/81116Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficasEl litio se ha usado por décadas como estabilizador del estado de ánimo en trastornos bipolares, pero sus mecanismos de acción a nivel celular aun no se han esclarecido. Aproximaciones bioquímicas resaltan la importancia, entre otras, de la vía de los fosfoinositoles. Nuestro laboratorio reportó efectos potenciadores del litio sobre la movilización de calcio intracelular y corrientes de membrana evocadas por activación de la vía de la fosfolipasa C (PLC) en líneas celulares: el sitio de acción fue acotado a la interacción entre proteína Gq y PLC. La existencia de variadas isoformas de estas proteínas en el sistema nervioso hace pertinente estudiar la generalidad de estos hallazgos en neuronas primarias. En el presente trabajo se implementó un cultivo de neuronas de cerebelo, ya que las neuronas de Purkinje expresan masivamente receptores de IP3 y distintas isoformas de PLC. Por razones de costo y fácil acceso, se utilizaron embriones de pollo. La selección de células candidatas se realizó mediante criterios morfológicos complementados por registros de ‘whole-cell voltage clamp’ que mostraron corrientes capacitivas lentas y de gran magnitud. Su viabilidad fue corroborada por la presencia de corrientes tanto voltaje-dependientes como activadas por quisquilato, un agonista de receptores glutamatérgicos acoplados a Gq/PLC. El quisquilato indujo además incrementos de calcio citosólico, provenientes en parte de reservorios intracelulares y sensibles a inhibición de la PLC. Finalmente, la exposición aguda a litio indujo reducción o potenciación de la respuesta de calcio en distintas células, sugiriendo un efecto diferencial del litio sobre variantes moleculares de PLC y/o Gq. (Texto tomado de la fuente).Lithium has been used for decades as a mood stabilizer in bipolar disorder, but its mechanisms of action at cellular level have not been clarified yet. Biochemical approaches highlight the importance, among others, of the phosphoinositides pathway. Our laboratory reported potentiating effects of lithium over the intracellular calcium mobilization and membrane currents evoked by the activation of the Phospholipase C (PLC) pathway in cell lines: the site of action was narrowed down to the interaction between the Gq protein and the PLC. The existence of diverse isoforms of these proteins in the nervous system makes pertinent to study the generality of these findings in primary neurons. In the present work a cerebellar neuronal culture was implemented, given that Purkinje neurons massively express IP3 receptors and diverse isoforms of PLC. Chick embryos were used for reasons of cost and easy access. The selection of candidate neurons was made by morphological criteria complemented by whole-cell voltage clamp recordings that showed slow capacitive currents with big amplitude. Cell viability was corroborated by the presence voltage-dependent currents as well as currents activated by quisqualate, an agonist of glutamatergic receptors coupled to Gq/PLC. Quisqualate induced cytosolic calcium increments, originated in part from intracellular reservoirs, and sensitive to PLC inhibition. Finally, exposure to acute lithium induced reduction or potentiation of the calcium response in different cells, suggesting a differential effect of lithium over molecular variants of PLC and/or Gq.MaestríaMagíster en Ciencias - BiologíaNeurofisiología celularxvi, 80 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BiologíaDepartamento de BiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::571 - Fisiología y temas relacionadosEnzymesPurkinje CellsEnzimasCélulas de PurkinjeNeuronsNeuronasLitioCélula de PurkinjeTrastorno bipolarEmbrión de polloProteína GqCultivo celular primarioFosfolipasa CLithiumPurkinje cellBipolar disorderChick embryoGq proteinPrimary cell culturePhospholipase CImplementación de un cultivo neuronal primario como modelo para el estudio de mecanismos de modulación sobre la vía de señalización de los fosfoinositolesImplementation of a primary neuronal culture as a model for the study of modulatory mechanisms on the phosphoinositide signaling pathwayTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAche, B. 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