Somatic embryogenesis in yam (dioscorea rotundata)
Embryogenic yam (Dioscorea rotundata) cultures were induced from petioles of leaves of in vitro grown plants on medium supplemented with different 2.4-D concentrations. Cultures were maintained either on semisolid or in liquid MS medium supplemented with 4.52 μM 2.4-D. The effect of sucrose concentr...
- Autores:
-
Suárez Padrón, Isidro Elías
Torres Arizal, Luz Angela
Litz, Richard
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2011
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/40421
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/40421
http://bdigital.unal.edu.co/30518/
- Palabra clave:
- Auxin
conversion
tropical species
food security
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| Summary: | Embryogenic yam (Dioscorea rotundata) cultures were induced from petioles of leaves of in vitro grown plants on medium supplemented with different 2.4-D concentrations. Cultures were maintained either on semisolid or in liquid MS medium supplemented with 4.52 μM 2.4-D. The effect of sucrose concentration on somatic embryo development was also evaluated and the effects of different BAP concentrations on somatic embryo conversion were determined. Treatments were distributed using a complete randomized design. The highest rate of induction occurred with 4.52 μM 2.4-D. Sucrose at 131.46 mM significantly enhanced somatic embryo development. The conversion rate was not affected by BAP. |
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