Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico
ilustraciones, fotografías, gráficas
- Autores:
-
Chona Perea, Rafael Esteban
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/83994
- Palabra clave:
- 610 - Medicina y salud::615 - Farmacología y terapéutica
Radioterapia/métodos
Radiación Ionizante
Supervivencia Celular/efectos de la radiación
Radiotherapy/methods
Radiation, Ionizing
Cell Survival/radiation effects
Radiobiología
Radioterapia
Cultivo Celula
Ensayo de supervivencia celular
Radiación ionizante
Radiobiology
Radioterapy
Cell suvirval Essay
Cell Culture
Ionazing radiation
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
| id |
UNACIONAL2_d031f1459f1a27d7703ad3a314ce6b47 |
|---|---|
| oai_identifier_str |
oai:repositorio.unal.edu.co:unal/83994 |
| network_acronym_str |
UNACIONAL2 |
| network_name_str |
Universidad Nacional de Colombia |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| dc.title.translated.eng.fl_str_mv |
Creation of a cell survival curve protocol for irradiated SiHa cells using a clinical linear accelerator |
| title |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| spellingShingle |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico 610 - Medicina y salud::615 - Farmacología y terapéutica Radioterapia/métodos Radiación Ionizante Supervivencia Celular/efectos de la radiación Radiotherapy/methods Radiation, Ionizing Cell Survival/radiation effects Radiobiología Radioterapia Cultivo Celula Ensayo de supervivencia celular Radiación ionizante Radiobiology Radioterapy Cell suvirval Essay Cell Culture Ionazing radiation |
| title_short |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| title_full |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| title_fullStr |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| title_full_unstemmed |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| title_sort |
Creación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínico |
| dc.creator.fl_str_mv |
Chona Perea, Rafael Esteban |
| dc.contributor.advisor.spa.fl_str_mv |
Rodríguez, Josefa Antonia Plazas, María Cristina |
| dc.contributor.author.spa.fl_str_mv |
Chona Perea, Rafael Esteban |
| dc.contributor.colaborator.spa.fl_str_mv |
Rumbo, Génesis Mejía, Vanessa Pabón, Alexandra |
| dc.subject.ddc.spa.fl_str_mv |
610 - Medicina y salud::615 - Farmacología y terapéutica |
| topic |
610 - Medicina y salud::615 - Farmacología y terapéutica Radioterapia/métodos Radiación Ionizante Supervivencia Celular/efectos de la radiación Radiotherapy/methods Radiation, Ionizing Cell Survival/radiation effects Radiobiología Radioterapia Cultivo Celula Ensayo de supervivencia celular Radiación ionizante Radiobiology Radioterapy Cell suvirval Essay Cell Culture Ionazing radiation |
| dc.subject.decs.spa.fl_str_mv |
Radioterapia/métodos Radiación Ionizante Supervivencia Celular/efectos de la radiación |
| dc.subject.decs.eng.fl_str_mv |
Radiotherapy/methods Radiation, Ionizing Cell Survival/radiation effects |
| dc.subject.proposal.spa.fl_str_mv |
Radiobiología Radioterapia Cultivo Celula Ensayo de supervivencia celular Radiación ionizante |
| dc.subject.proposal.eng.fl_str_mv |
Radiobiology Radioterapy Cell suvirval Essay Cell Culture Ionazing radiation |
| description |
ilustraciones, fotografías, gráficas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-06-07T19:19:02Z |
| dc.date.available.none.fl_str_mv |
2023-06-07T19:19:02Z |
| dc.date.issued.none.fl_str_mv |
2023-06-04 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/83994 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/83994 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.indexed.spa.fl_str_mv |
Bireme |
| dc.relation.references.spa.fl_str_mv |
Faiz M Khan and John P Gibbons. Khan’s the physics of radiation therapy. Lippincott Williams & Wilkins, 2014. CS Sureka and Christina Armpilia. Radiation biology for medical physicists. CRC Press, 2017. Geoff Delaney, Susannah Jacob, Carolyn Featherstone, and Michael Barton. The role of radiotherapy in cancer treatment: estimating optimal utilization from a review of evidence-based clinical guidelines. Cancer: Interdisciplinary International Journal of the American Cancer Society, 104(6):1129–1137, 2005. Paulo Nuno Martins. A brief history about radiotherapy. International Journal of Latest Research in Engineering and Technology (IJLRET), 4, 2018. Bo Lindell. A history of radiation protection. Radiation protection dosimetry, 68(1- 2):83–95, 1996. Elaine M Zeman. The history and radiobiology of hypofractionation. In Hypofractionated and Stereotactic Radiation Therapy, pages 1–31. Springer, 2018. Stephen Joseph McMahon. The linear quadratic model: usage, interpretation and challenges. Physics in Medicine & Biology, 64(1):01TR01, 2018. David G Kirsch, Max Diehn, Aparna H Kesarwala, Amit Maity, Meredith A Morgan, Julie K Schwarz, Robert Bristow, Sandra Demaria, Iris Eke, Robert J Griffin, et al. The future of radiobiology. JNCI: Journal of the National Cancer Institute, 110(4):329–340, 2018. Jing Hao, Anthony Magnelli, Andrew Godley, and Jennifer S Yu. Use of a linear accelerator for conducting in vitro radiobiology experiments. Journal of visualized experiments: JoVE, (147), 2019. Brita Singers Sørensen, Anne Vestergaard, Jens Overgaard, and Lars Hjorth Præstegaard. Dependence of cell survival on instantaneous dose rate of a linear accelerator. Radiotherapy and Oncology, 101(1):223–225, 2011. Paulo Alejandro Quintero Mej´ıa. Estudio in vitro de la supervivencia en c´elulas tumorales irradiadas con un acelerador lineal de uso cl´ınico. Departamento de F´ısica. United Nations Scientific Committee on the Effects of Atomic Radiation et al. Sources, effects and risks of ionizing radiation. 1988. Pedro Andreo, David T Burns, Alan E Nahum, Jan Seuntjens, and Frank Herbert Attix. Fundamentals of ionizing radiation dosimetry. John Wiley & Sons, 2017. Ervin B Podgorˇsak et al. Radiation physics for medical physicists, volume 1. Springer, 2006. Meenakshi Arora. Cell culture media: a review. Mater methods, 3(175):24, 2013. R Ian Freshney. Culture of animal cells: a manual of basic technique and specialized applications. John Wiley & Sons, 2015. Donald F Swinehart. The beer-lambert law. Journal of chemical education, 39(7):333, 1962. Robert W Ricci, Mauri Ditzler, and Lisa P Nestor. Discovering the beer-lambert law. Journal of chemical Education, 71(11):983, 1994. Mesoionic compounds are defined by IUPAC. as “dipolar five-(possibly six-) membered heterocyclic compounds in which both the negative and the positive charge are delocalized, for which a totally covalent structure cannot be written, and which cannot be represented satisfactorily by any one polar structure. the formal positive charge is associated with the ring atoms, and the formal negative charge is associated with ring atoms or an exocyclic nitrogen or chalcogen atom”. iupac. compendium of chemical terminology, 2nd edn. gold book compiled by ad mcnaught and a. wilkinson. Compendium of Chemical Terminology, 2nd Ed. Gold Book Compiled, 1997. Johan Van Meerloo, Gertjan JL Kaspers, and Jacqueline Cloos. Cell sensitivity assays: the mtt assay. In Cancer cell culture, pages 237–245. Springer, 2011. O Garz´on, Plazas Plazas, Maria Cristina, and EJ Salazar. Evolution of physicomathematical models in radiobiology and their application in ionizing radiation therapies. Tecciencia, 9(17):15–22, 2014. Magnus Strandqvist. Studien ¨uber die kumulative Wirkung der R¨ontgenstrahlen bei Fraktionierung: Erfahrungen aus dem Radiumhemmet an 280 Haut-und Lippenkarzinomen. Norstedt, 1944. JF Fowler. The estimation of total dose for different numbers of fractions in radiotherapy. The British Journal of radiology, 38(449):365–368, 1965. F Ellis. I. the dose-time relationship in radiotherapy and the validity of cell survival curve models. The British Journal of Radiology, 36(423):153–162, 1963. Frank Ellis. Dose, time and fractionation: a clinical hypothesis. Clinical radiology, 20(1):1–7, 1969. Miguel Gil Gayarre, M Delgado Garc´ıa, and MOSC Mart´ınez Morillo. Manual de radiolog´ıa cl´ınica, 1994. L Jones, P Hoban, and P Metcalfe. The use of the linear quadratic model in radiotherapy: a review. Australasian Physics & Engineering Sciences in Medicine, 24(3):132–146, 2001. Luis Medina, Daniel Venencia, and Edgardo Garrig´o. Qa imrt comparison specific patient by means of radiochromic films, radiographic films and ionization chambers arrangement. 2014. Warren Strober. Trypan blue exclusion test of cell viability. Current protocols in immunology, 21(1):A–3B, 1997. James Carmichael, William G DeGraff, Adi F Gazdar, John D Minna, and James B Mitchell. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer research, 47(4):936–942, 1987. Anieta M Sieuwerts, Jan GM Klijn, Harry A Peters, and John A Foekens. The mtt tetrazolium salt assay scrutinized: How to use this assay reliably to measure metabolie activity of cell cultures in vitro for the assessment of growth characteristics, ic50-values and cell survival. 1995. A Slavotinek, TJ McMillan, and CM Steel. Measurement of radiation survival using the mtt assay. European Journal of Cancer, 30(9):1376–1382, 1994. Peter R Twentyman and Mark Luscombe. A study of some variables in a tetrazolium dye (mtt) based assay for cell growth and chemosensitivity. British journal of cancer, 56(3):279–285, 1987. Nicolaas AP Franken, Hans M Rodermond, Jan Stap, Jaap Haveman, and Chris Van Bree. Clonogenic assay of cells in vitro. Nature protocols, 1(5):2315–2319, 2006. Nicolaas AP Franken, Hans M Rodermond, Jan Stap, Jaap Haveman, and Chris Van Bree. Clonogenic assay of cells in vitro. Nature protocols, 1(5):2315–2319, 2006. Jane A Plumb. Cell sensitivity assays: clonogenic assay. In Cancer cell culture, pages 159–164. Springer, 2004. Md Asabur Rahman, Nasrin Sultana, Ummay Ayman, Sonali Bhakta, Marzia Afrose, Marya Afrin, and Ziaul Haque. Alcoholic fixation over formalin fixation: A new, safer option for morphologic and molecular analysis of tissues. Saudi journal of biological sciences, 29(1):175–182, 2022. Jing Hao, Anthony Magnelli, Andrew Godley, and S Yu Jennifer. Use of a linear accelerator for conducting in vitro radiobiology experiments. JoVE (Journal of Visualized Experiments), (147):e59514, 2019. Paul Reid, Puthenparampil Wilson, Yanrui Li, Loredana G Marcu, Alexander H Staudacher, Michael P Brown, and Eva Bezak. Experimental investigation of radiobiology in head and neck cancer cell lines as a function of hpv status, by mtt assay. Scientific Reports, 8(1):1–7, 2018. Adriana Agudelo, Oscar Ortega, Paulo Quintero, Alejandro Ondo, and Maria Cristina. Evaluaci´on del efecto de la hipoxia en la supervivencia de celulas tumorales y endoteliales sometidas a radiaciones ionizantes. Karl Buch, Tanja Peters, Thomas Nawroth, Markus S¨anger, Heinz Schmidberger, and Peter Langguth. Determination of cell survival after irradiation via clonogenic assay versus multiple mtt assay-a comparative study. Radiation oncology, 7(1):1–6, 2012. Uttam K Sinha, Victor J Schorn, Christian Hochstim, Steven B Chinn, Sutao Zhu, and Rizwan Masood. Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition. Head & neck, 33(2):178–188, 2011.v Serra Kamer, Qing Ren, and Adam P Dicker. Differential radiation sensitization of human cervical cancer cell lines by the proteasome inhibitor velcade (bortezomib, ps-341). Archives of gynecology and obstetrics, 279(1):41–46, 2009. Kana Aoki, Shinsuke Satoi, Shota Harada, Seiichi Uchida, Yoh Iwasa, and Junichi Ikenouchi. Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb. Molecular biology of the cell, 31(8):833–844, 2020. |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.license.spa.fl_str_mv |
Reconocimiento 4.0 Internacional |
| dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Reconocimiento 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.extent.spa.fl_str_mv |
70 páginas |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.publisher.program.spa.fl_str_mv |
Bogotá - Ciencias - Maestría en Física Médica |
| dc.publisher.faculty.spa.fl_str_mv |
Facultad de Ciencias |
| dc.publisher.place.spa.fl_str_mv |
Bogotá, Colombia |
| dc.publisher.branch.spa.fl_str_mv |
Universidad Nacional de Colombia - Sede Bogotá |
| institution |
Universidad Nacional de Colombia |
| bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/83994/2/1098796024.2023.pdf https://repositorio.unal.edu.co/bitstream/unal/83994/1/license.txt https://repositorio.unal.edu.co/bitstream/unal/83994/3/1098796024.2023.pdf.jpg |
| bitstream.checksum.fl_str_mv |
a38aa8f903cf74fb1d3eec1a84a63171 eb34b1cf90b7e1103fc9dfd26be24b4a b8e0dc5bc2046141e7349de32894534b |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
| _version_ |
1814089830040076288 |
| spelling |
Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rodríguez, Josefa Antonia76f507b35ecb2c04dbaf12a36def5210Plazas, María Cristina3a0ca95e85d6f86f5d25028ada0b1516500Chona Perea, Rafael Estebandbc28c52fd5f49b4fbd2c42183d416b1Rumbo, GénesisMejía, VanessaPabón, Alexandra2023-06-07T19:19:02Z2023-06-07T19:19:02Z2023-06-04https://repositorio.unal.edu.co/handle/unal/83994Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficasA finales del siglo XIX, poco después de la invención de los rayos X, se descubrió que la radiación ionizante podía ser utilizada como tratamiento oncológico. Durante el siguiente siglo se realizaron una serie de experimentos in vivo e in vitro, que buscaban explicar las propiedades de la interacción radiación-célula, creando el campo de la radiobiología. A partir de estas investigaciones se desarrolló el modelo lineal-cuadrático, que sirve para cuantificar el daño letal y sub-letal de la radiación en la célula, comparando la dosis recibida con la fracción de supervivencia celular. Esto se conoce como una curva de supervivencia celular y es muy útil para comparar distintas características de un tratamiento en radioterapia (como fraccionamiento, calidad de la radiación, entre otras). En este trabajo final de maestría se busca crear un protocolo de construcción de curvas de supervivencia celular utilizando haces de radiación de 6 MeV con un acelerador lineal para facilitar la realización de experimentos radiobiólogicos en el futuro. (Texto tomado de la fuente).At the end of the XIX century, right after the invention of xrays, it was discovered that ionizing radiation could be used as oncological treatment. During the following century a lot of advancements were made trying to understand that connection, in particular a lot of in vivo and in vitro experiments were made improving our understanding of the interaction of radiation and the cell, creating the field of Radiobiology. Using that research as a starting point the Lineal quadratic model was created, to attempt to quantify the lethal and sublethal damage produce to the cell by quantifying the cellular death with the dose prescribed. This is what is know as Cell Survival curve and it is very useful to define the reaction of tissue and cells after being expose to radiation. This study aims to develop a guide to create cell survival curves using what is known as an MTT assay for determining the impact of the radiation in the cell.MaestríaMagíster en Física Médica70 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Física MédicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::615 - Farmacología y terapéuticaRadioterapia/métodosRadiación IonizanteSupervivencia Celular/efectos de la radiaciónRadiotherapy/methodsRadiation, IonizingCell Survival/radiation effectsRadiobiologíaRadioterapiaCultivo CelulaEnsayo de supervivencia celularRadiación ionizanteRadiobiologyRadioterapyCell suvirval EssayCell CultureIonazing radiationCreación de un protocolo para la construcción de curvas de supervivencia sobre la línea celular SiHa utilizando un acelerador lineal de uso clínicoCreation of a cell survival curve protocol for irradiated SiHa cells using a clinical linear acceleratorTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBiremeFaiz M Khan and John P Gibbons. Khan’s the physics of radiation therapy. Lippincott Williams & Wilkins, 2014.CS Sureka and Christina Armpilia. Radiation biology for medical physicists. CRC Press, 2017.Geoff Delaney, Susannah Jacob, Carolyn Featherstone, and Michael Barton. The role of radiotherapy in cancer treatment: estimating optimal utilization from a review of evidence-based clinical guidelines. Cancer: Interdisciplinary International Journal of the American Cancer Society, 104(6):1129–1137, 2005.Paulo Nuno Martins. A brief history about radiotherapy. International Journal of Latest Research in Engineering and Technology (IJLRET), 4, 2018.Bo Lindell. A history of radiation protection. Radiation protection dosimetry, 68(1- 2):83–95, 1996.Elaine M Zeman. The history and radiobiology of hypofractionation. In Hypofractionated and Stereotactic Radiation Therapy, pages 1–31. Springer, 2018.Stephen Joseph McMahon. The linear quadratic model: usage, interpretation and challenges. Physics in Medicine & Biology, 64(1):01TR01, 2018.David G Kirsch, Max Diehn, Aparna H Kesarwala, Amit Maity, Meredith A Morgan, Julie K Schwarz, Robert Bristow, Sandra Demaria, Iris Eke, Robert J Griffin, et al. The future of radiobiology. JNCI: Journal of the National Cancer Institute, 110(4):329–340, 2018.Jing Hao, Anthony Magnelli, Andrew Godley, and Jennifer S Yu. Use of a linear accelerator for conducting in vitro radiobiology experiments. Journal of visualized experiments: JoVE, (147), 2019.Brita Singers Sørensen, Anne Vestergaard, Jens Overgaard, and Lars Hjorth Præstegaard. Dependence of cell survival on instantaneous dose rate of a linear accelerator. Radiotherapy and Oncology, 101(1):223–225, 2011.Paulo Alejandro Quintero Mej´ıa. Estudio in vitro de la supervivencia en c´elulas tumorales irradiadas con un acelerador lineal de uso cl´ınico. Departamento de F´ısica.United Nations Scientific Committee on the Effects of Atomic Radiation et al. Sources, effects and risks of ionizing radiation. 1988.Pedro Andreo, David T Burns, Alan E Nahum, Jan Seuntjens, and Frank Herbert Attix. Fundamentals of ionizing radiation dosimetry. John Wiley & Sons, 2017.Ervin B Podgorˇsak et al. Radiation physics for medical physicists, volume 1. Springer, 2006.Meenakshi Arora. Cell culture media: a review. Mater methods, 3(175):24, 2013.R Ian Freshney. Culture of animal cells: a manual of basic technique and specialized applications. John Wiley & Sons, 2015.Donald F Swinehart. The beer-lambert law. Journal of chemical education, 39(7):333, 1962.Robert W Ricci, Mauri Ditzler, and Lisa P Nestor. Discovering the beer-lambert law. Journal of chemical Education, 71(11):983, 1994.Mesoionic compounds are defined by IUPAC. as “dipolar five-(possibly six-) membered heterocyclic compounds in which both the negative and the positive charge are delocalized, for which a totally covalent structure cannot be written, and which cannot be represented satisfactorily by any one polar structure. the formal positive charge is associated with the ring atoms, and the formal negative charge is associated with ring atoms or an exocyclic nitrogen or chalcogen atom”. iupac. compendium of chemical terminology, 2nd edn. gold book compiled by ad mcnaught and a. wilkinson. Compendium of Chemical Terminology, 2nd Ed. Gold Book Compiled, 1997.Johan Van Meerloo, Gertjan JL Kaspers, and Jacqueline Cloos. Cell sensitivity assays: the mtt assay. In Cancer cell culture, pages 237–245. Springer, 2011.O Garz´on, Plazas Plazas, Maria Cristina, and EJ Salazar. Evolution of physicomathematical models in radiobiology and their application in ionizing radiation therapies. Tecciencia, 9(17):15–22, 2014.Magnus Strandqvist. Studien ¨uber die kumulative Wirkung der R¨ontgenstrahlen bei Fraktionierung: Erfahrungen aus dem Radiumhemmet an 280 Haut-und Lippenkarzinomen. Norstedt, 1944.JF Fowler. The estimation of total dose for different numbers of fractions in radiotherapy. The British Journal of radiology, 38(449):365–368, 1965.F Ellis. I. the dose-time relationship in radiotherapy and the validity of cell survival curve models. The British Journal of Radiology, 36(423):153–162, 1963.Frank Ellis. Dose, time and fractionation: a clinical hypothesis. Clinical radiology, 20(1):1–7, 1969.Miguel Gil Gayarre, M Delgado Garc´ıa, and MOSC Mart´ınez Morillo. Manual de radiolog´ıa cl´ınica, 1994.L Jones, P Hoban, and P Metcalfe. The use of the linear quadratic model in radiotherapy: a review. Australasian Physics & Engineering Sciences in Medicine, 24(3):132–146, 2001.Luis Medina, Daniel Venencia, and Edgardo Garrig´o. Qa imrt comparison specific patient by means of radiochromic films, radiographic films and ionization chambers arrangement. 2014.Warren Strober. Trypan blue exclusion test of cell viability. Current protocols in immunology, 21(1):A–3B, 1997.James Carmichael, William G DeGraff, Adi F Gazdar, John D Minna, and James B Mitchell. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer research, 47(4):936–942, 1987.Anieta M Sieuwerts, Jan GM Klijn, Harry A Peters, and John A Foekens. The mtt tetrazolium salt assay scrutinized: How to use this assay reliably to measure metabolie activity of cell cultures in vitro for the assessment of growth characteristics, ic50-values and cell survival. 1995.A Slavotinek, TJ McMillan, and CM Steel. Measurement of radiation survival using the mtt assay. European Journal of Cancer, 30(9):1376–1382, 1994.Peter R Twentyman and Mark Luscombe. A study of some variables in a tetrazolium dye (mtt) based assay for cell growth and chemosensitivity. British journal of cancer, 56(3):279–285, 1987.Nicolaas AP Franken, Hans M Rodermond, Jan Stap, Jaap Haveman, and Chris Van Bree. Clonogenic assay of cells in vitro. Nature protocols, 1(5):2315–2319, 2006.Nicolaas AP Franken, Hans M Rodermond, Jan Stap, Jaap Haveman, and Chris Van Bree. Clonogenic assay of cells in vitro. Nature protocols, 1(5):2315–2319, 2006.Jane A Plumb. Cell sensitivity assays: clonogenic assay. In Cancer cell culture, pages 159–164. Springer, 2004.Md Asabur Rahman, Nasrin Sultana, Ummay Ayman, Sonali Bhakta, Marzia Afrose, Marya Afrin, and Ziaul Haque. Alcoholic fixation over formalin fixation: A new, safer option for morphologic and molecular analysis of tissues. Saudi journal of biological sciences, 29(1):175–182, 2022.Jing Hao, Anthony Magnelli, Andrew Godley, and S Yu Jennifer. Use of a linear accelerator for conducting in vitro radiobiology experiments. JoVE (Journal of Visualized Experiments), (147):e59514, 2019.Paul Reid, Puthenparampil Wilson, Yanrui Li, Loredana G Marcu, Alexander H Staudacher, Michael P Brown, and Eva Bezak. Experimental investigation of radiobiology in head and neck cancer cell lines as a function of hpv status, by mtt assay. Scientific Reports, 8(1):1–7, 2018.Adriana Agudelo, Oscar Ortega, Paulo Quintero, Alejandro Ondo, and Maria Cristina. Evaluaci´on del efecto de la hipoxia en la supervivencia de celulas tumorales y endoteliales sometidas a radiaciones ionizantes.Karl Buch, Tanja Peters, Thomas Nawroth, Markus S¨anger, Heinz Schmidberger, and Peter Langguth. Determination of cell survival after irradiation via clonogenic assay versus multiple mtt assay-a comparative study. Radiation oncology, 7(1):1–6, 2012.Uttam K Sinha, Victor J Schorn, Christian Hochstim, Steven B Chinn, Sutao Zhu, and Rizwan Masood. Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition. Head & neck, 33(2):178–188, 2011.vSerra Kamer, Qing Ren, and Adam P Dicker. Differential radiation sensitization of human cervical cancer cell lines by the proteasome inhibitor velcade (bortezomib, ps-341). Archives of gynecology and obstetrics, 279(1):41–46, 2009.Kana Aoki, Shinsuke Satoi, Shota Harada, Seiichi Uchida, Yoh Iwasa, and Junichi Ikenouchi. Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb. Molecular biology of the cell, 31(8):833–844, 2020.EstudiantesInvestigadoresPúblico generalORIGINAL1098796024.2023.pdf1098796024.2023.pdfTesis de Maestría en Física Médicaapplication/pdf7493534https://repositorio.unal.edu.co/bitstream/unal/83994/2/1098796024.2023.pdfa38aa8f903cf74fb1d3eec1a84a63171MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83994/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL1098796024.2023.pdf.jpg1098796024.2023.pdf.jpgGenerated Thumbnailimage/jpeg6325https://repositorio.unal.edu.co/bitstream/unal/83994/3/1098796024.2023.pdf.jpgb8e0dc5bc2046141e7349de32894534bMD53unal/83994oai:repositorio.unal.edu.co:unal/839942024-08-11 01:06:18.976Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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 |