Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)

ilustraciones, diagramas, tablas

Autores:: Perez Pazos, Jazmin Vanessa

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
dc.title.translated.eng.fl_str_mv	Standardization of in vitro and ex vitro production conditions for the propagation and scaling of sweet potato (Ipomoea batatas L.) planting material
title	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
spellingShingle	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.) 570 - Biología 580 - Plantas 630 - Agricultura y tecnologías relacionadas Batata - Propagación in vitro Compost Plant micropropagation Micropropagación vegetal Cultivo de tejidos Aclimatación Medio de cultivo Sustratos Super elite Mini-raíces tuberosas Propagación Tissue culture Acclimatization Culture medium Substrates Super elite Tuberous mini-roots Propagation
title_short	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
title_full	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
title_fullStr	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
title_full_unstemmed	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
title_sort	Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)
dc.creator.fl_str_mv	Perez Pazos, Jazmin Vanessa
dc.contributor.advisor.none.fl_str_mv	Rosero Alpala, Elvia Amparo Gamez Carrillo, Rocio Margarita Hoyos Sánchez, Rodrigo Alberto
dc.contributor.author.none.fl_str_mv	Perez Pazos, Jazmin Vanessa
dc.subject.ddc.spa.fl_str_mv	570 - Biología 580 - Plantas 630 - Agricultura y tecnologías relacionadas
topic	570 - Biología 580 - Plantas 630 - Agricultura y tecnologías relacionadas Batata - Propagación in vitro Compost Plant micropropagation Micropropagación vegetal Cultivo de tejidos Aclimatación Medio de cultivo Sustratos Super elite Mini-raíces tuberosas Propagación Tissue culture Acclimatization Culture medium Substrates Super elite Tuberous mini-roots Propagation
dc.subject.lemb.none.fl_str_mv	Batata - Propagación in vitro Compost Plant micropropagation Micropropagación vegetal
dc.subject.proposal.spa.fl_str_mv	Cultivo de tejidos Aclimatación Medio de cultivo Sustratos Super elite Mini-raíces tuberosas Propagación
dc.subject.proposal.eng.fl_str_mv	Tissue culture Acclimatization Culture medium Substrates Super elite Tuberous mini-roots Propagation
description	ilustraciones, diagramas, tablas
publishDate	2022
dc.date.accessioned.none.fl_str_mv	2022-10-25T14:49:57Z
dc.date.available.none.fl_str_mv	2022-10-25T14:49:57Z
dc.date.issued.none.fl_str_mv	2022
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/82447
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/82447 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional
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rights_invalid_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2
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dc.format.extent.spa.fl_str_mv	xxv, 136 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Medellín - Ciencias - Maestría en Ciencias - Biotecnología
dc.publisher.department.spa.fl_str_mv	Escuela de biociencias
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rosero Alpala, Elvia Amparodbea913b044c94a76be918e8daede0f7Gamez Carrillo, Rocio Margarita956a2c5c728ba056926180c82634fd16600Hoyos Sánchez, Rodrigo Alberto6ddb39f31a5436d96ecc1e671ae2131e600Perez Pazos, Jazmin Vanessafaa40601a0466ff91b02b71d428fb0a56002022-10-25T14:49:57Z2022-10-25T14:49:57Z2022https://repositorio.unal.edu.co/handle/unal/82447Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, tablasLa batata (Ipomoea batatas L.) es un cultivo de alta importancia en seguridad alimentaria por sus propiedades nutracéuticas. La disponibilidad de material de siembra libre de enfermedades asegura el éxito en el establecimiento, rendimiento y calidad del cultivo. La micropropagación in vitro es una alternativa eficaz para obtener material vegetal con calidad fitosanitaria. El objetivo de este estudio fue estandarizar las condiciones de producción in vitro y ex vitro para la propagación y escalado de material de siembra de batata En condiciones de laboratorio se evaluaron estrategias de desinfección, caracterizaron microorganismos contaminantes y determinó la influencia de reguladores de crecimiento sobre plántulas in vitro. En invernadero y vivero se evaluaron mezclas de sustratos a base de turba (T), lombriabono (LA), cascarilla de arroz (CA), fibra de coco (FC) y arena fina (A) para mejorar la producción de material de siembra. Los resultados indicaron que lavado con yodopovidona y desinfección con hipoclorito de sodio al 2%, ácido acético y amonio cuaternario permitió erradicar el 70% de contaminantes. de los géneros Fusarium, Sarocladium, Cladosporium, Aspergillus, Pseudozyma, Moesziomyces y Curtobacterium. Los explantes cultivados con ANA (0,2ppm) presentaron una mayor producción de nudos y crecimiento aéreo significativamente superior al control. Para la aclimatación de vitroplantas, el sustrato compuesto por T:LA:CA (3:1:1) y cámara húmeda por 8 días permitió obtener plántulas super elite con buen crecimiento, altas tasas de supervivencia (92%) y multiplicación (3,53) en comparación con la estrategia convencional (turba, sin cámara húmeda). En condiciones de vivero, sustrato de A:LA:FC (7:1:2), produjo exitosamente hasta 79 mini-raíces/m2. Una mayor eficiencia en la producción de material de siembra de alta calidad con condiciones fitosanitarias garantizadas es una contribución importante para mejorar las estrategias globales de manejo de enfermedades en el cultivo de batata. (Texto tomado de la fuente)Sweet potato (Ipomoea batatas L.) is a highly important crop for food security due to its nutraceutical properties. The availability of disease-free planting material ensures successful establishment, yield, and quality of the crop. In vitro micropropagation is an effective alternative to obtain plant material with phytosanitary quality. The objective of this study was to standardize the in vitro and ex vitro production conditions for the propagation and scaling of sweet potato planting material. In laboratory conditions, disinfection strategies were evaluated, contaminating microorganisms were characterized and the influence of growth regulators on the development of the in vitro plant was determined. In the greenhouse and nursery, mixtures of substrates based on peat (T), vermicompost (LA), rice husks (CA), coconut fiber (FC) and fine sand (A) were evaluated to improve the production of planting material. The results indicated that washing with povidone-iodine and disinfection with 2% sodium hypochlorite, acetic acid and quaternary ammonium eradicated 70% of contaminants. of the genera Fusarium, Sarocladium, Cladosporium, Aspergillus, Pseudozyma, Moesziomyces and Curtobacterium. The explants cultured with ANA (0.2ppm) showed a higher production of nodes and aerial growth significantly higher than the control. For the acclimatization of vitroplants, the substrate composed of T:LA:CA (3:1:1) and a humid chamber for 8 days allowed obtaining super elite seedlings with good growth, high survival rates (92%) and multiplication (3, 53) compared to the conventional strategy (peat, without humid chamber). Under nursery conditions, A:LA:FC (7:1:2) substrate successfully produced up to 79 mini-roots/m2. Greater efficiency in the production of high-quality planting material with guaranteed phytosanitary conditions is an important contribution to improve global disease management strategies in sweet potato cultivation.Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA (Ejecutor)MaestríaMagíster en Ciencias - BiotecnologíaÁrea Curricular Biotecnologíaxxv, 136 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaEscuela de biocienciasFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - Biología580 - Plantas630 - Agricultura y tecnologías relacionadasBatata - Propagación in vitroCompostPlant micropropagationMicropropagación vegetalCultivo de tejidosAclimataciónMedio de cultivoSustratosSuper eliteMini-raíces tuberosasPropagaciónTissue cultureAcclimatizationCulture mediumSubstratesSuper eliteTuberous mini-rootsPropagationEstandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)Standardization of in vitro and ex vitro production conditions for the propagation and scaling of sweet potato (Ipomoea batatas L.) planting materialTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbad, M., Noguera, P., & Burés, S. 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Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8461-6_20Desarrollo de estrategias de manejo integrado del cultivo, postcosecha y aprovechamiento integral del cultivo de batata en Colombia.Ministerio de Agricultura y Desarrollo RuralEstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/82447/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1085282821.2022.pdf1085282821.2022.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf5539329https://repositorio.unal.edu.co/bitstream/unal/82447/2/1085282821.2022.pdfb412efbee591aacc4e7cb2645a5a16feMD52THUMBNAIL1085282821.2022.pdf.jpg1085282821.2022.pdf.jpgGenerated Thumbnailimage/jpeg5856https://repositorio.unal.edu.co/bitstream/unal/82447/3/1085282821.2022.pdf.jpg2d76eb2a8861d6c0ce209b3ef4ce04b7MD53unal/82447oai:repositorio.unal.edu.co:unal/824472023-08-10 23:03:49.204Repositorio Institucional Universidad Nacional de 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Estandarización de condiciones de producción in vitro y ex vitro para la propagación y escalamiento de material de siembra de batata (Ipomoea batatas L.)

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