Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación
ilustraciones, fotografías , graficas
- Autores:
-
Morales Cortés, Yenny Paola
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/81366
- Palabra clave:
- 660 - Ingeniería química::662 - Tecnología de explosivos, combustibles, productos relacionados
Equilibrio ácido-base
Biomasas
Afifella marina
Hidrógeno
Fotofermentación
Ácidos orgánicos
Bórax
KPI
Hydrogen
Photofermentation
Organic acids
KPI
Borax
Afifella marina
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| dc.title.translated.eng.fl_str_mv |
Evaluation of growth conditions and hydrogen production of Afifella marina using industrial waste as a substrate through photofermentation |
| dc.title.translated.fra.fl_str_mv |
Évaluation des conditions de croissance et de la production d'hydrogène d'Afifella marina en utilisant des déchets industriels comme substrat par photofermentation |
| title |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| spellingShingle |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación 660 - Ingeniería química::662 - Tecnología de explosivos, combustibles, productos relacionados Equilibrio ácido-base Biomasas Afifella marina Hidrógeno Fotofermentación Ácidos orgánicos Bórax KPI Hydrogen Photofermentation Organic acids KPI Borax Afifella marina |
| title_short |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| title_full |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| title_fullStr |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| title_full_unstemmed |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| title_sort |
Evaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentación |
| dc.creator.fl_str_mv |
Morales Cortés, Yenny Paola |
| dc.contributor.advisor.none.fl_str_mv |
Serrato Bermúdez, Juan Carlos Magnin, Jean Pierre |
| dc.contributor.author.none.fl_str_mv |
Morales Cortés, Yenny Paola |
| dc.contributor.other.none.fl_str_mv |
Castillo Moreno, Patricia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Procesos Químicos y Bioquímicos |
| dc.subject.ddc.spa.fl_str_mv |
660 - Ingeniería química::662 - Tecnología de explosivos, combustibles, productos relacionados |
| topic |
660 - Ingeniería química::662 - Tecnología de explosivos, combustibles, productos relacionados Equilibrio ácido-base Biomasas Afifella marina Hidrógeno Fotofermentación Ácidos orgánicos Bórax KPI Hydrogen Photofermentation Organic acids KPI Borax Afifella marina |
| dc.subject.other.spa.fl_str_mv |
Equilibrio ácido-base Biomasas |
| dc.subject.proposal.spa.fl_str_mv |
Afifella marina Hidrógeno Fotofermentación Ácidos orgánicos Bórax KPI |
| dc.subject.proposal.eng.fl_str_mv |
Hydrogen Photofermentation Organic acids KPI Borax Afifella marina |
| description |
ilustraciones, fotografías , graficas |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-24T17:23:50Z |
| dc.date.available.none.fl_str_mv |
2022-03-24T17:23:50Z |
| dc.date.issued.none.fl_str_mv |
2022-03-23 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/81366 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/81366 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Serrato Bermúdez, Juan Carlos2cec2f7270f1d605a516d2e2beace5a2Magnin, Jean Pierre2699250f9f19a79e37e300c43b532ea0Morales Cortés, Yenny Paolad69f417ba3de8cb2a7ec8c7a942f4516Castillo Moreno, PatriciaGrupo de Investigación en Procesos Químicos y Bioquímicos2022-03-24T17:23:50Z2022-03-24T17:23:50Z2022-03-23https://repositorio.unal.edu.co/handle/unal/81366Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías , graficasEn el presente trabajo se evaluó la producción de hidrógeno a partir de la bacteria Afifella marina utilizando un medio sintético con composición similar a un residuo de fermentación oscura de la paja de trigo con una concentración total de ácidos de 112 mM. Este medio contenía ácidos orgánicos tales como ácido láctico y ácido acético los cuales fueron utilizados por la bacteria como principal fuente de carbono en un proceso de fotofermentación. Para establecer las condiciones óptimas de crecimiento de la bacteria y producción de hidrógeno, se estudiaron en medios sintéticos algunas variables significativas para el proceso: concentración y relación de ácidos orgánicos, fuente de nitrógeno, intensidad lumínica, agitación, concentración de sal y solución amortiguadora en botellas de 120 mL. La verificación de la presencia de hidrógeno en el biogás producido se realizó por medio de un cromatógrafo de gases de la serie 5890 de Hewlett Packard (serie A), con detector de conductividad térmica (TCD) obteniendo un 95% de hidrógeno (H2) en las muestras procesadas, el 5% restante se caracterizó como dióxido de carbono (CO2). Inicialmente se empleó un diseño de mezcla simplex lattice para escoger la mejor relación de los ácidos estudiados y posteriormente un diseño central compuesto rotable para evaluar el efecto sobre la producción de hidrógeno de tres variables continuas: relación de la concentración de la fuente de carbono (ácido láctico y ácido acético, concentración total 112 mM), concentración de fuente de nitrógeno (glutamato entre 3.5 y 13 mM) e intensidad lumínica (entre 6,000 y 18,000 lux); y una variable discreta (solución amortiguadora (medio LGK y medio LGB). La relación de ácidos orgánicos con la que se presentó la mejor producción de biogás para la solución amortiguadora LGK fue: 60 mM ácido acético, 60 mM ácido láctico con una producción total de 169 mL de biogás, mientras que para la solución amortiguadora LGB la mejor relación de ácidos orgánicos fue: 20 mM ácido acético, 80 mM ácido láctico, 20 mM de ácido butírico con una producción total de 200 mL de biogás. Además, se determinó que las mejores condiciones para la producción de biogás en la solución amortiguadora LGK fueron: una concentración de ácidos totales de 76 mM, una concentración de glutamato de 0.26 mM y una intensidad lumínica de 12.000 lux con un rendimiento de 19.08% respecto al ácido láctico y ácido acético. Para la solución amortiguadora LGB las condiciones que presentaron la mejor producción de biogás fueron: una concentración de ácidos totales de 76 mM, una concentración de glutamato de 8.25 mM y una intensidad lumínica de 12.000 lux con un rendimiento de 44.13% respecto al ácido láctico y ácido acético. Por último, se realizó un escalado de los experimentos con las dos soluciones amortiguadoras LGK y LGB en reactores de 1 L. Por cada solución amortiguadora se evaluaron dos reactores en los cuales se probaron las mejores condiciones obtenidas previamente: concentración total de ácidos 76 y 112 mM, concentración de glutamato 3.5 y 8.25 mM, intensidad lumínica 4,000 lux, obteniendo que la solución amortiguadora LGB presentó la mayor producción de biogás (concentración total de ácidos 112 mM, concentración de glutamato 3.50 mM) con una producción máxima de 4,139 mL de biogás/L. (Texto tomado de la fuente)In the present study, the hydrogen production by the photosynthetic bacterium Afifella marina was evaluated using a synthetic medium with a similar composition to a residue dark fermentation of wheat straw. This medium contained organic acids such as lactic acid acetic and butyric acid with a total acid organic concentration of 112 mM which were used by the bacteria as the main carbon source in a photofermentation process. Optimal conditions for growth and hydrogen production were established by DOE methodology. Continuous and discontinuous variables were studied in synthetic media: concentration and ratio of organic acids, nitrogen source, light intensity, stirring, salt concentration, and buffer solution. Produced hydrogen contained 95% hydrogen (H2) and 5% of carbon dioxide (CO2). A two-step procedure was carried out with the statistical software Design-Expert a simplex lattice mixture design for the selection of the best acids ratio studied and a central composite sphere-type design to evaluate the effect on hydrogen production of three continuous variables: concentration ratio of lactic acid and acetic acid (total concentration 112 mM), nitrogen source concentration as Na Glutamate (3.50 and 13 mM) and light intensity (range 6,000 -18,000 lux); and one discrete variable as: buffer solution (LGK and LGB medium). The best ratio of organic acid in LGK (KPI buffer) and LGB (Borax buffer) for maximal H2 production was 60 mM acetic acid, 60 mM lactic acid with a total production of 161 mL H2 and 20 mM acetic acid, 80 mM lactic acid, 20 mM butyric acid with a total production of 190 mL H2 respectively. Maximal H2 production, in the second part of the study was determined. The best conditions to produce biogas in the solution LGK buffer were: a total acid concentration of 76 mM, a glutamate concentration of 0.26 mM and a light intensity of 12.000 lux with a yield of 19.08% for lactic acid and acetic acid. For the LGB buffer solution, the conditions that presented the best biogas production were a total acid concentration of 76 mM, a glutamate concentration of 8.25 mM, and a light intensity of 12.000 lux with a yield of 44.13% concerning lactic acid and acetic acid. At the end of the project, the experiments were scaled up with the two buffer solutions LGK and LGB in 1 L reactors. For each buffer solution, two reactors were evaluated in which the best conditions obtained in the previous experiment designs were tested, the conditions in The ones in which these experiments were carried out were: total acid concentration 76 and 112 mM, glutamate concentration 3.50 and 8.25 mM, light intensity 4,000 lux, obtaining that the LGB buffer solution presented the highest biogas production (total acid concentration 112 mM, glutamate concentration 3.50 mM) with a total volume of 4,139 mL of biogas.MaestríaMagíster en Ingeniería - Ingeniería QuímicaquímBioprocesosxxii, 152 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ingeniería - Maestría en Ingeniería - Ingeniería QuímicaDepartamento de Ingeniería Química y AmbientalFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá660 - Ingeniería química::662 - Tecnología de explosivos, combustibles, productos relacionadosEquilibrio ácido-baseBiomasasAfifella marinaHidrógenoFotofermentaciónÁcidos orgánicosBóraxKPIHydrogenPhotofermentationOrganic acidsKPIBoraxAfifella marinaEvaluación de las condiciones de crecimiento y producción de hidrógeno de Afifella marina utilizando residuos industriales como sustrato por medio de fotofermentaciónEvaluation of growth conditions and hydrogen production of Afifella marina using industrial waste as a substrate through photofermentationÉvaluation des conditions de croissance et de la production d'hydrogène d'Afifella marina en utilisant des déchets industriels comme substrat par photofermentationTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMEIA, “About 7% of fossil fuels are consumed for non-combustion use in the United States - Today in Energy - U.S. Energy Information Administration (EIA),” U.S Energy Information Administration, Apr. 06, 2018. https://www.eia.gov/todayinenergy/detail.php?id=35672 (accessed Sep. 13, 2021)H. 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Available: https://es.slideshare.net/yuricomartinez/labo2-peso-hmedo-peso-seco-turbidimetra.EstudiantesInvestigadoresPersonal de apoyo escolarPúblico generalORIGINAL1014238643.2022.pdf1014238643.2022.pdfTesis de Maestría en Ingeniería Químicaapplication/pdf4821763https://repositorio.unal.edu.co/bitstream/unal/81366/3/1014238643.2022.pdff3a95af91eac62fc2ea961229a577303MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81366/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1014238643.2022.pdf.jpg1014238643.2022.pdf.jpgGenerated Thumbnailimage/jpeg5962https://repositorio.unal.edu.co/bitstream/unal/81366/5/1014238643.2022.pdf.jpg4185644305e9b8e3d96a8ce2378c94deMD55unal/81366oai:repositorio.unal.edu.co:unal/813662023-08-03 23:04:22.889Repositorio Institucional Universidad Nacional de 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