Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca
ilustraciones, diagramas, fotografías
- Autores:
-
García Rico, Danna Lorena
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85343
- Palabra clave:
- 660 - Ingeniería química
570 - Biología
Residuos
Waste Products
Gestión de Residuos
Penicillium sp.
Calcio
Casaminoácidos
Aireación
Bioprocesos
Degradación
Flores
Calcium
Casamino acids
Aeration
Bioprocess
Degradation
Flowers
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| dc.title.translated.eng.fl_str_mv |
Evaluation of the effect of growing conditions on the production of Penicillium sp. HC1 used in the degradation of plant residues from flower crops in Guasca - Cundinamarca |
| title |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| spellingShingle |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca 660 - Ingeniería química 570 - Biología Residuos Waste Products Gestión de Residuos Penicillium sp. Calcio Casaminoácidos Aireación Bioprocesos Degradación Flores Calcium Casamino acids Aeration Bioprocess Degradation Flowers |
| title_short |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| title_full |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| title_fullStr |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| title_full_unstemmed |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| title_sort |
Evaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – Cundinamarca |
| dc.creator.fl_str_mv |
García Rico, Danna Lorena |
| dc.contributor.advisor.none.fl_str_mv |
Moreno Sarmiento, Nubia Carmenza Gutiérrez Rojas, Ivonne |
| dc.contributor.author.none.fl_str_mv |
García Rico, Danna Lorena |
| dc.contributor.researchgroup.spa.fl_str_mv |
Bioprocesos y Bioprospección |
| dc.subject.ddc.spa.fl_str_mv |
660 - Ingeniería química 570 - Biología |
| topic |
660 - Ingeniería química 570 - Biología Residuos Waste Products Gestión de Residuos Penicillium sp. Calcio Casaminoácidos Aireación Bioprocesos Degradación Flores Calcium Casamino acids Aeration Bioprocess Degradation Flowers |
| dc.subject.decs.none.fl_str_mv |
Residuos |
| dc.subject.decs.eng.fl_str_mv |
Waste Products |
| dc.subject.decs.spa.fl_str_mv |
Gestión de Residuos |
| dc.subject.proposal.spa.fl_str_mv |
Penicillium sp. Calcio Casaminoácidos Aireación Bioprocesos Degradación Flores |
| dc.subject.proposal.eng.fl_str_mv |
Calcium Casamino acids Aeration Bioprocess Degradation Flowers |
| description |
ilustraciones, diagramas, fotografías |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023-12-05 |
| dc.date.accessioned.none.fl_str_mv |
2024-01-17T14:23:59Z |
| dc.date.available.none.fl_str_mv |
2024-01-17T14:23:59Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85343 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/85343 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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García Mora, A. E. (2006). Estudio de la degradación de residuos lignocelulósicos derivados del procesamiento industrial del cranberry (Vaccinium macrocarpon Ait.). http://cybertesis.uach.cl/tesis/uach/2006/egg216e/doc/egg216e.pdf García-Rico, R. O., Martín, J. F., & Fierro, F. (2011). Heterotrimeric Gα protein Pga1 from Penicillium chrysogenum triggers germination in response to carbon sources and affects negatively resistance to different stress conditions. Fungal Genetics and Biology, 48(6), 641–649. https://doi.org/10.1016/j.fgb.2010.11.013 García-Soto, M. J., Botello-Alvarez, E., Jiménez-Islas, H., Navarrete-Bolaños, J. L., Barajas-Conde, E., Rico-Martínez, R., Guevara-González, R. G., & Torres-Pacheco, I. (2006). Growth morphology and hydrodynamics of filamentous fungi in submerged cultures. Germec, M., & Turhan, I. (2023). Effect of pH control and aeration on inulinase production from sugarbeet molasses in a bench-scale bioreactor. 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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Moreno Sarmiento, Nubia Carmenza410ae8ab86963ff6ed5de426293858da600Gutiérrez Rojas, Ivonneb1d760973fb2da6a9aaa6f67959cc4be600García Rico, Danna Lorenac96eb7b18a0d6403f19f7cfc9bca79d6Bioprocesos y Bioprospección2024-01-17T14:23:59Z2024-01-17T14:23:59Z2023-12-05https://repositorio.unal.edu.co/handle/unal/85343Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasEn las últimas décadas, Colombia se ha posicionado como un referente a nivel mundial en la industria floricultora. Sin embargo, este próspero sector enfrenta retos ambientales, siendo la generación de residuos una preocupación constante. En contexto, la producción de desechos de clavel en el país es de 1500 kg/mes por hectárea, de los cuales en promedio el 10% es utilizado en compostaje y el 90% restante es dado por lo general como alimento para animales, lo cual ha sido cuestionable por magnificar los plaguicidas en la cadena trófica. Es así, como la búsqueda de soluciones sostenibles y eficientes para la degradación de residuos vegetales se ha convertido en una prioridad de este sector agrícola. Una alternativa que se ha venido estudiando es el uso directo de microorganismos con capacidad lignocelulolítica. Biocultivos S.A. y el Instituto de Biotecnología de la Universidad Nacional de Colombia – IBUN desarrollaron un inóculo a partir de Penicillium sp. HC1 para acelerar la degradación de material lignocelulósico. Este bioinsumo presenta desafíos alineados a la producción y calidad de sus conidios en fermentación sumergida y, por tanto, el presente trabajo evaluó diferentes condiciones de cultivo sobre la producción de Penicillium sp. HC1, así como su uso en la degradación de residuos de material vegetal de cultivos de residuos de clavel en búsqueda de la mejora del producto. Esta investigación no mostró cambios significativos en el producto final utilizando CaCl2. Por otra parte, se profundizó en la influencia que tienen diversos aditivos complejos como casaminoácidos, aminoácidos ramificados, otros suplementos y vitaminas al ser agregados al medio de cultivo líquido en la producción de Penicillium sp. HC1, encontrándose resultados positivos. Concentraciones de 13 g/L de casaminoácidos mejoran la producción de biomasa y conidios 6% y 11% respectivamente con relación al control (medio base). Del mismo modo, los llamados aminoácidos de prueba (AP) en esta investigación, resultaron ser un reemplazo efectivo y más económico a los casaminoácidos, obteniendo además mejoría en la viabilidad y productividad de los conidios de Penicillium sp. HC1. Sin embargo, la tolerancia térmica no mostraba progreso. Esto se solucionó al adicionar vitaminas obteniendo un incremento del 64% de la tolerancia térmica con respecto al valor obtenido con el producto inicial. Adicionalmente, se exploró el efecto que tienen tres flujos aire en el proceso de fermentación sumergida en tanque agitado, logrando una mejoría significativa en el producto a 2,5 vvm. En resumen, la biomasa mejoró un 27%, los conidios 16%, la viabilidad 76% y la tolerancia térmica 54% en el producto del biorreactor. Por último, se validó el uso de Penicillium sp. HC1 proveniente del medio de cultivo MBAP VIT producido a 2,5 vvm, satisfaciendo así una necesidad agrícola en la industria floricultora, que busca constantemente alternativas para el manejo de sus residuos vegetales. Esta investigación demostró la mejora en la aceleración de la degradación de los residuos de clavel utilizando Penicillium sp. HC1, consiguiendo resultados favorables en co-cultivos con Pleurotus ostreatus T1.1. (Texto tomado de la fuente)In recent decades, Colombia has positioned itself as a world leader in the flower industry. However, this thriving sector faces environmental challenges, with waste generation being a constant concern. In context, carnation waste production in the country is 1500 kg/month per hectare, of which an average of 10% is used in composting and the remaining 90% is usually given as animal feed, which has been questioned for magnifying pesticides in the food chain. Thus, the search for sustainable and efficient solutions for the degradation of plant residues has become a priority in this agricultural sector. One alternative that has been studied is the direct use of microorganisms with lignocellulolytic capacity. Biocultivos S.A. and the Institute of Biotechnology of the National University of Colombia - IBUN developed an inoculum from Penicillium sp. HC1 to accelerate the degradation of lignocellulosic material. This bioinput presents challenges aligned to the production and quality of its conidia in submerged fermentation and, therefore, the present work evaluated different culture conditions on the production of Penicillium sp. HC1, as well as its use in the degradation of plant material residues from carnation residue crops in search of product improvement. This research did not show significant changes in the final product using CaCl2. On the other hand, the influence of various complex additives such as casamino acids, branched amino acids, other supplements and vitamins when added to the liquid culture medium on the production of Penicillium sp. HC1 was studied, and positive results were found. Concentrations of 13 g/L of casamino acids improve the production of biomass and conidia 6% and 11%, respectively, with respect to the control (base medium). Similarly, the so-called test amino acids (PA) in this research proved to be an effective and more economical replacement for casamino acids, also improving the viability and productivity of Penicillium sp. HC1 conidia. However, thermal tolerance did not show progress. This was solved by adding vitamins, obtaining a 64% increase in thermal tolerance with respect to the initial product. Additionally, the effect of three air flows in the submerged fermentation process in a stirred tank was explored, achieving a significant improvement in the product at 2.5 vvm. In summary, biomass improved 27%, conidia 16%, viability 76% and thermal tolerance 54% in the bioreactor product. Finally, the use of Penicillium sp. HC1 from the MBAP VIT culture medium produced at 2.5 vvm was validated, thus satisfying an agricultural need in the floriculture industry, which is constantly looking for alternatives for the management of its plant residues. This research demonstrated the improvement in the acceleration of carnation waste degradation using Penicillium sp. HC1, achieving favorable results in co-cultures with Pleurotus ostreatus T1.1.MaestríaMagíster en Ciencias - MicrobiologíaBioinsumosxvii, 149 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá660 - Ingeniería química570 - BiologíaResiduosWaste ProductsGestión de ResiduosPenicillium sp.CalcioCasaminoácidosAireaciónBioprocesosDegradaciónFloresCalciumCasamino acidsAerationBioprocessDegradationFlowersEvaluación del efecto de condiciones de cultivo sobre la producción de Penicillium sp. HC1 usado en la degradación de residuos vegetales de cultivos de flores en Guasca – CundinamarcaEvaluation of the effect of growing conditions on the production of Penicillium sp. 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Molecules, 23(1), 125. https://doi.org/10.3390/molecules23010125EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85343/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1069305875.2023.pdf1069305875.2023.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf3815572https://repositorio.unal.edu.co/bitstream/unal/85343/2/1069305875.2023.pdfcc5151eb527c903ff1ebc56f10d9358eMD52THUMBNAIL1069305875.2023.pdf.jpg1069305875.2023.pdf.jpgGenerated Thumbnailimage/jpeg5775https://repositorio.unal.edu.co/bitstream/unal/85343/3/1069305875.2023.pdf.jpga2b4e726f83d606ab17a88caee062601MD53unal/85343oai:repositorio.unal.edu.co:unal/853432024-08-21 23:13:15.717Repositorio Institucional Universidad Nacional de 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