Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites

Secondary metabolites, particularly antibiotics, are among the most relevant pharmaceutical compounds worldwide and they are generally produced by submerged cultivation of filamentous fungi. From the hydrodynamical point of view, these processes deal with the dispersion of up to four different phase...

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Autores:
Corkidi, Gabriel
Serrano-Carreón, Leobardo
Galindo, Enrique
Tipo de recurso:
Article of journal
Fecha de publicación:
2008
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/22743
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/22743
http://bdigital.unal.edu.co/13778/
Palabra clave:
cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
micelial cultures
hydrodynamics
multiphasic dispersión
microbial physiology
productivity
image analysis
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_cd34a65018cb689dd5f0d9761c6cc76d
oai_identifier_str oai:repositorio.unal.edu.co:unal/22743
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
title Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
spellingShingle Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
micelial cultures
hydrodynamics
multiphasic dispersión
microbial physiology
productivity
image analysis
title_short Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
title_full Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
title_fullStr Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
title_full_unstemmed Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
title_sort Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites
dc.creator.fl_str_mv Corkidi, Gabriel
Serrano-Carreón, Leobardo
Galindo, Enrique
dc.contributor.author.spa.fl_str_mv Corkidi, Gabriel
Serrano-Carreón, Leobardo
Galindo, Enrique
dc.subject.proposal.spa.fl_str_mv cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
micelial cultures
hydrodynamics
multiphasic dispersión
microbial physiology
productivity
image analysis
topic cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
cultivos miceliares
hidrodinámica
dispersión multifásica
fisiología microbiana
productividad
micelial cultures
hydrodynamics
multiphasic dispersión
microbial physiology
productivity
image analysis
description Secondary metabolites, particularly antibiotics, are among the most relevant pharmaceutical compounds worldwide and they are generally produced by submerged cultivation of filamentous fungi. From the hydrodynamical point of view, these processes deal with the dispersion of up to four different phases: water-oil-air-fungi. It is well recognized that hydrodynamics has a significant influence on the global performance of the fermentation process; however, very little is understood about the relationships between hydrodynamics-dispersion-physiology-productivity. This poor understanding is partially due to the lack of precise methodologies to assess fungal viability (determining both the yield and productivity of the metabolite) and the size of the air bubbles and oil drops (related to the nutrient transport efficiency like oxygen and fatty acids). In this work, the published developments of our group, regarding image analysis-based methodologies for the study of Trichoderma harzianum cultivation for antimycotics production (v.g. 6-pentyl-alpha-pyrone) have been summarized and revised. The application of such methodologies resulted in the improvement of basic knowledge of the phenomena occurring within the fermenter as well as in the development of strategies for increasing the productivity of these processes. Key words: micelial cultures; hydrodynamics; multiphasic dispersión; microbial physiology; productivity; image analysis.
publishDate 2008
dc.date.issued.spa.fl_str_mv 2008
dc.date.accessioned.spa.fl_str_mv 2019-06-25T20:40:52Z
dc.date.available.spa.fl_str_mv 2019-06-25T20:40:52Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.content.spa.fl_str_mv Text
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format http://purl.org/coar/resource_type/c_6501
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dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/22743
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/13778/
url https://repositorio.unal.edu.co/handle/unal/22743
http://bdigital.unal.edu.co/13778/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/biotecnologia/article/view/1398
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología
Revista Colombiana de Biotecnología
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Biotecnología; Vol. 10, núm. 1 (2008); 75-93 1909-8758 0123-3475
dc.relation.references.spa.fl_str_mv Corkidi, Gabriel and Serrano-Carreón, Leobardo and Galindo, Enrique (2008) Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites. Revista Colombiana de Biotecnología; Vol. 10, núm. 1 (2008); 75-93 1909-8758 0123-3475 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/22743/1/1398-6741-1-PB.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Corkidi, Gabriel73877a10-7c30-47ae-b06b-4ae1e0896036300Serrano-Carreón, Leobardo16562e17-79ca-4227-bf2d-38445695526a300Galindo, Enrique550e6c8e-55a2-482a-8a9c-3e9c4a385f433002019-06-25T20:40:52Z2019-06-25T20:40:52Z2008https://repositorio.unal.edu.co/handle/unal/22743http://bdigital.unal.edu.co/13778/Secondary metabolites, particularly antibiotics, are among the most relevant pharmaceutical compounds worldwide and they are generally produced by submerged cultivation of filamentous fungi. From the hydrodynamical point of view, these processes deal with the dispersion of up to four different phases: water-oil-air-fungi. It is well recognized that hydrodynamics has a significant influence on the global performance of the fermentation process; however, very little is understood about the relationships between hydrodynamics-dispersion-physiology-productivity. This poor understanding is partially due to the lack of precise methodologies to assess fungal viability (determining both the yield and productivity of the metabolite) and the size of the air bubbles and oil drops (related to the nutrient transport efficiency like oxygen and fatty acids). In this work, the published developments of our group, regarding image analysis-based methodologies for the study of Trichoderma harzianum cultivation for antimycotics production (v.g. 6-pentyl-alpha-pyrone) have been summarized and revised. The application of such methodologies resulted in the improvement of basic knowledge of the phenomena occurring within the fermenter as well as in the development of strategies for increasing the productivity of these processes. Key words: micelial cultures; hydrodynamics; multiphasic dispersión; microbial physiology; productivity; image analysis.Los metabolitos secundarios, y particularmente los antibióticos, se encuentran entre el grupo de fármacos de mayor relevancia en el mercado mundial, y son producidos, en su mayoría, mediante el cultivo sumergido de hongos filamentosos. Desde el punto de vista hidrodinámico, estos procesos involucran fundamentalmente la dispersión de hasta cuatro fases diferentes: agua-aceite-aire-hongo. Aun cuando se sabe que la hidrodinámica del cultivo determina la eficiencia global del proceso de fermentación, poco se ha logrado en cuanto al entendimiento de las relaciones hidrodinámica-dispersión-fisiología-productividad. Esto se debe en parte a la falta de metodologías que permitan cuantificar y caracterizar con precisión tanto la viabilidad del hongo (ya que en buena medida determina el rendimiento y la productividad del metabolito de interés), como el tamaño de las burbujas de gas y de gotas de aceite (ya que de ello depende la eficiencia de transporte de nutrientes como el oxígeno y los ácidos grasos del aceite). En este trabajo se resume y revisa el desarrollo de las metodologías que nuestro grupo ha publicado, basadas en el procesamiento y el análisis digital de imágenes aplicadas al estudio del cultivo de Trichoderma harzianum, hongo que produce antimicóticos de alta potencia (como la 6-pentil-alfa-pirona). Con el uso de estas metodologías hemos generado conocimiento básico de los fenómenos que ocurren en el fermentador, lo que nos ha permitido establecer estrategias para incrementar la productividad de este tipo de procesos. Palabras clave: cultivos miceliares; hidrodinámica; dispersión multifásica; fisiología microbiana; productividad; análisis de imágenesapplication/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/1398Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de BiotecnologíaRevista Colombiana de BiotecnologíaRevista Colombiana de Biotecnología; Vol. 10, núm. 1 (2008); 75-93 1909-8758 0123-3475Corkidi, Gabriel and Serrano-Carreón, Leobardo and Galindo, Enrique (2008) Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolites. Revista Colombiana de Biotecnología; Vol. 10, núm. 1 (2008); 75-93 1909-8758 0123-3475 .Digital image analysis for microscope characterisation of the critical parameters involved in the fermentative production of secondary metabolitesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTcultivos miceliareshidrodinámicadispersión multifásicafisiología microbianaproductividadcultivos miceliareshidrodinámicadispersión multifásicafisiología microbianaproductividadmicelial cultureshydrodynamicsmultiphasic dispersiónmicrobial physiologyproductivityimage analysisORIGINAL1398-6741-1-PB.pdfapplication/pdf2544366https://repositorio.unal.edu.co/bitstream/unal/22743/1/1398-6741-1-PB.pdf27e312833868c9944c3a15d5c2328b25MD51THUMBNAIL1398-6741-1-PB.pdf.jpg1398-6741-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg6526https://repositorio.unal.edu.co/bitstream/unal/22743/2/1398-6741-1-PB.pdf.jpg8d50f9377585c1f04228e4a922ddfe20MD52unal/22743oai:repositorio.unal.edu.co:unal/227432023-10-08 23:05:14.367Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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