In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis

Given the importance and potential development of avocado (Persea americana Mill.) cultivation in Colombia and its problems for an efficient field cultivation, which includes the genetic variability of propagated material and adverse phytosanitary conditions, the aim of this study was to develop a m...

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Autores:
Restrepo Osorio, Catalina
Gómez Velásquez, Felipe Andrés
Gil Correal, Alejandro
Torres Bonilla, Javier Mauricio
Urrea Trujillo, Aura Ines
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/68129
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/68129
http://bdigital.unal.edu.co/69162/
Palabra clave:
55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Acclimatization
etiolation
in vitro
greenhouse
photoperiodicity
plant propagation, rooting
Endurecimiento
enraizamiento
etiolización
fotoperiodicidad
in vitro
invernadero
propagación vegetal
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_ccf5ee3ffd692cec19117487a8ff8d72
oai_identifier_str oai:repositorio.unal.edu.co:unal/68129
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
title In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
spellingShingle In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Acclimatization
etiolation
in vitro
greenhouse
photoperiodicity
plant propagation, rooting
Endurecimiento
enraizamiento
etiolización
fotoperiodicidad
in vitro
invernadero
propagación vegetal
title_short In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
title_full In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
title_fullStr In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
title_full_unstemmed In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
title_sort In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis
dc.creator.fl_str_mv Restrepo Osorio, Catalina
Gómez Velásquez, Felipe Andrés
Gil Correal, Alejandro
Torres Bonilla, Javier Mauricio
Urrea Trujillo, Aura Ines
dc.contributor.author.spa.fl_str_mv Restrepo Osorio, Catalina
Gómez Velásquez, Felipe Andrés
Gil Correal, Alejandro
Torres Bonilla, Javier Mauricio
Urrea Trujillo, Aura Ines
dc.subject.ddc.spa.fl_str_mv 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
topic 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Acclimatization
etiolation
in vitro
greenhouse
photoperiodicity
plant propagation, rooting
Endurecimiento
enraizamiento
etiolización
fotoperiodicidad
in vitro
invernadero
propagación vegetal
dc.subject.proposal.spa.fl_str_mv Acclimatization
etiolation
in vitro
greenhouse
photoperiodicity
plant propagation, rooting
Endurecimiento
enraizamiento
etiolización
fotoperiodicidad
in vitro
invernadero
propagación vegetal
description Given the importance and potential development of avocado (Persea americana Mill.) cultivation in Colombia and its problems for an efficient field cultivation, which includes the genetic variability of propagated material and adverse phytosanitary conditions, the aim of this study was to develop a method for in vitro clonal propagation for avocado cv. Hass. In vitro introduction of apical explants and nodal segments was carried out from grafted parent plants. Etiolation pretreatment effect on the number of shoots formed and in vitro phenolization was evaluated in grafted parent plants and in explants under in vitro conditions, subject to photoperiod (12/12 hours) and darkness. Darkness conditions reduced significantly plant phenolization, and these in turn formed new shoots, which were higher and were also formed in a shorter time. During multiplication stage, using a WPM culture medium an effective control of phenolization was obtained. During subculturing there was a decreasing in apical explant elongation, but there was a significant induction of new shoots from nodal segments. However, only some segments reached the required height to keep the multiplication rate throughout subcultures. Finally, 82% survival rate of vitroplants was obtained during hardening.
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018-01-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T05:56:03Z
dc.date.available.spa.fl_str_mv 2019-07-03T05:56:03Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.content.spa.fl_str_mv Text
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dc.identifier.issn.spa.fl_str_mv ISSN: 2323-0118
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/68129
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/69162/
identifier_str_mv ISSN: 2323-0118
url https://repositorio.unal.edu.co/handle/unal/68129
http://bdigital.unal.edu.co/69162/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/61474
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
dc.relation.references.spa.fl_str_mv Restrepo Osorio, Catalina and Gómez Velásquez, Felipe Andrés and Gil Correal, Alejandro and Torres Bonilla, Javier Mauricio and Urrea Trujillo, Aura Ines (2018) In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis. Acta Agronómica, 67 (1). pp. 160-167. ISSN 2323-0118
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Palmira
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/68129/1/61474-362472-8-PB.pdf
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Restrepo Osorio, Catalina635390b7-8f11-4125-869c-990e35741167300Gómez Velásquez, Felipe Andrés1589ed1f-79e3-4b2b-bb4a-5eab592c1eb3300Gil Correal, Alejandro62a35bd6-b090-4d8e-b167-0ff64cc387d1300Torres Bonilla, Javier Mauricio16235dd5-0d38-4087-ab97-fe6f94b976c3300Urrea Trujillo, Aura Ines744fd232-c848-48f2-ad9f-800c49da9c303002019-07-03T05:56:03Z2019-07-03T05:56:03Z2018-01-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/68129http://bdigital.unal.edu.co/69162/Given the importance and potential development of avocado (Persea americana Mill.) cultivation in Colombia and its problems for an efficient field cultivation, which includes the genetic variability of propagated material and adverse phytosanitary conditions, the aim of this study was to develop a method for in vitro clonal propagation for avocado cv. Hass. In vitro introduction of apical explants and nodal segments was carried out from grafted parent plants. Etiolation pretreatment effect on the number of shoots formed and in vitro phenolization was evaluated in grafted parent plants and in explants under in vitro conditions, subject to photoperiod (12/12 hours) and darkness. Darkness conditions reduced significantly plant phenolization, and these in turn formed new shoots, which were higher and were also formed in a shorter time. During multiplication stage, using a WPM culture medium an effective control of phenolization was obtained. During subculturing there was a decreasing in apical explant elongation, but there was a significant induction of new shoots from nodal segments. However, only some segments reached the required height to keep the multiplication rate throughout subcultures. Finally, 82% survival rate of vitroplants was obtained during hardening.Teniendo en cuenta la importancia y el potencial de desarrollo del cultivo de aguacate (Persea americana Mill.) en Colombia, y los problemas que se presentan para el cultivo eficiente en campo, principalmente por la variabilidad genética del material de propagación y por condiciones fitosanitarias adversas, el presente trabajo tuvo como finalidad desarrollar una metodología para la propagación clonal in vitro del cultivar “Hass”. La introducción in vitro de explantes apicales y segmentos nodales se realizó a partir de plantas madres injertadas. El efecto del pretratamiento de etiolación sobre el número de brotes desarrollados y el porcentaje de fenolización in vitro, se evaluó sometiendo las plantas madre y los explantes introducidos in vitro a condiciones de fotoperiodo (12/12h) y oscuridad; esta última condición es la que redujo significativamente la fenolización de los explantes, y estos a su vez formaron un mayor número de brotes nuevos con mayor altura y en menor tiempo. En la etapa de multiplicación en el medio de cultivo WPM se obtuvo un efectivo control del proceso de fenolización. Durante los subcultivos hubo disminución de elongación de los explantes apicales, y a partir de los segmentos nodales, se logró una inducción importante de nuevos brotes; sin embargo, solo algunos alcanzaron la altura requerida para mantener la tasa de multiplicación a través de los subcultivos. Finalmente durante el endurecimiento, se obtuvo un 82 % de supervivencia de las vitroplantas.application/pdfspaUniversidad Nacional de Colombia - Sede Palmirahttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/61474Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaRestrepo Osorio, Catalina and Gómez Velásquez, Felipe Andrés and Gil Correal, Alejandro and Torres Bonilla, Javier Mauricio and Urrea Trujillo, Aura Ines (2018) In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis. Acta Agronómica, 67 (1). pp. 160-167. ISSN 2323-011855 Ciencias de la tierra / Earth sciences and geology63 Agricultura y tecnologías relacionadas / AgricultureAcclimatizationetiolationin vitrogreenhousephotoperiodicityplant propagation, rootingEndurecimientoenraizamientoetiolizaciónfotoperiodicidadin vitroinvernaderopropagación vegetalIn vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesisArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL61474-362472-8-PB.pdfapplication/pdf13075659https://repositorio.unal.edu.co/bitstream/unal/68129/1/61474-362472-8-PB.pdfc7b8fa77cf9553846276a65913aaca92MD51THUMBNAIL61474-362472-8-PB.pdf.jpg61474-362472-8-PB.pdf.jpgGenerated Thumbnailimage/jpeg6631https://repositorio.unal.edu.co/bitstream/unal/68129/2/61474-362472-8-PB.pdf.jpge2bb8d18cba4163e5f15580c8d9cc918MD52unal/68129oai:repositorio.unal.edu.co:unal/681292023-06-02 23:03:32.224Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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