Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos
ilustraciones, diagramas
- Autores:
-
Carabali Mosquera, Oscar Eduardo
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84272
- Palabra clave:
- 570 - Biología::576 - Genética y evolución
Klebsiella pneumoniae
Pseudomonas aeruginosa
Taxa
Secuencia de ADN
DNA sequences
Secuenciación completa del genoma
Klebsiella pneumoniae
Pseudomonas aeruginosa
Tipificación molecular bacteriana
Herramientas Bioinformáticas
Benchmarking
Whole genome sequencing
Klebsiella pneumoniae
Pseudomonas aeruginosa
Bacterial molecular typing
Bioinformatics tools
Benchmarking
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| dc.title.translated.eng.fl_str_mv |
Evaluation of Bioinformatic tools useful in the typing of Klebsiella pneumoniae and Pseudomonas aeruginosa from whole genome sequencing data |
| title |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| spellingShingle |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos 570 - Biología::576 - Genética y evolución Klebsiella pneumoniae Pseudomonas aeruginosa Taxa Secuencia de ADN DNA sequences Secuenciación completa del genoma Klebsiella pneumoniae Pseudomonas aeruginosa Tipificación molecular bacteriana Herramientas Bioinformáticas Benchmarking Whole genome sequencing Klebsiella pneumoniae Pseudomonas aeruginosa Bacterial molecular typing Bioinformatics tools Benchmarking |
| title_short |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| title_full |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| title_fullStr |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| title_full_unstemmed |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| title_sort |
Evaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completos |
| dc.creator.fl_str_mv |
Carabali Mosquera, Oscar Eduardo |
| dc.contributor.advisor.none.fl_str_mv |
Barreto Hernández, Emiliano |
| dc.contributor.author.none.fl_str_mv |
Carabali Mosquera, Oscar Eduardo |
| dc.contributor.researchgroup.spa.fl_str_mv |
Bioinformática |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::576 - Genética y evolución |
| topic |
570 - Biología::576 - Genética y evolución Klebsiella pneumoniae Pseudomonas aeruginosa Taxa Secuencia de ADN DNA sequences Secuenciación completa del genoma Klebsiella pneumoniae Pseudomonas aeruginosa Tipificación molecular bacteriana Herramientas Bioinformáticas Benchmarking Whole genome sequencing Klebsiella pneumoniae Pseudomonas aeruginosa Bacterial molecular typing Bioinformatics tools Benchmarking |
| dc.subject.agrovoc.none.fl_str_mv |
Klebsiella pneumoniae Pseudomonas aeruginosa |
| dc.subject.agrovoc.spa.fl_str_mv |
Taxa Secuencia de ADN |
| dc.subject.agrovoc.eng.fl_str_mv |
DNA sequences |
| dc.subject.proposal.spa.fl_str_mv |
Secuenciación completa del genoma Klebsiella pneumoniae Pseudomonas aeruginosa Tipificación molecular bacteriana Herramientas Bioinformáticas Benchmarking Whole genome sequencing |
| dc.subject.proposal.eng.fl_str_mv |
Klebsiella pneumoniae Pseudomonas aeruginosa Bacterial molecular typing Bioinformatics tools Benchmarking |
| description |
ilustraciones, diagramas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-07-26T12:54:22Z |
| dc.date.available.none.fl_str_mv |
2023-07-26T12:54:22Z |
| dc.date.issued.none.fl_str_mv |
2023-06-15 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84272 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84272 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Barreto Hernández, Emilianob7a2cae2c08b5d6a549e173576c6c82dCarabali Mosquera, Oscar Eduardoc2631a483d68ccbb092c28a7e6d09420Bioinformática2023-07-26T12:54:22Z2023-07-26T12:54:22Z2023-06-15https://repositorio.unal.edu.co/handle/unal/84272Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasLa Secuencia del Genoma Completo se obtiene mediante las tecnologías de secuenciación, especialmente las de próxima generación (NGS). Gracias a su alto poder discriminatorio, simplicidad, precisión, velocidad y flexibilidad, la aplicación de la Secuenciación de Genoma Completo (WGS) se ha convertido en una herramienta que aporta el nivel más alto hasta el momento de discriminación de cepas bacterianas para la investigación de brotes, permitiendo identificar estructura y composición de genes, variantes genéticas y reordenamientos del genoma entre otros (Kwong et al., 2015). Por tal motivo, esta herramienta es de gran utilidad para la investigación epidemiológica ya que proporciona información más detallada y precisa para la toma oportuna de medidas de control derivado de la identificación, tipificación, dinámica de transmisión, procedencia de infección y posibles patrones de propagación de brotes con bacterias como Klebsiella pneumoniae y Pseudomonas aeruginosa causantes de Infecciones Asociadas a la Atención en Salud (IAAS), esto dado que estas bacterias poseen un alto grado de adaptabilidad fisiológica y elevados niveles de resistencia frente a numerosos agentes antimicrobianos, por lo que son patógenos con una elevada incidencia de morbilidad y mortalidad (Saharman et al., 2019)(Moradigaravand et al., 2017). Dentro del uso más frecuente de los datos de Secuenciación de Genoma Completo (WGS) se encuentra la tipificación molecular bacteriana. Por la cual, se han desarrollado varios métodos que se basan principalmente en análisis derivados de Ribosomal Multilocus Sequence Typing (rMLST), Core genome multilocus sequence typing (cgMLST), Whole genome multi locus sequence typing (wgMLST), core genome single nucleotide polymorphism (cgSNP), whole-genome single nucleotide polymorphism (wgSNP) y pangenome (Coll et al., 2020)(Anani et al., 2020). Estos métodos pueden variar en su resolución e idoneidad dependiendo de las especies. Sin embargo, debido al variado número de herramientas bioinformáticas útiles para tipificar y a la falta de consenso de evaluación comparativa de las herramientas los investigadores se pueden enfrentan con dificultades en la elección de herramientas indicada para sus actividades. Es por ello, que se hace necesario realizar una evaluación del desempeño de las herramientas útiles para tipificar, con el fin de informar al usuario sobre las mejores herramientas bioinformáticas disponibles actualmente que brinden información precisa y relevante. (Texto tomado de la fuente)Whole Genome Sequence is obtained by sequencing technologies, especially nextgeneration sequencing (NGS). Due to its high discriminatory power, simplicity, precision, speed, and flexibility, the application of Whole Genome Sequencing (WGS) has become a tool that provides the highest level of discrimination of bacterial strains for outbreak investigation to date, allowing to identify both, structure and composition of genes, genetic variants, and rearrangements of the genome among others (Kwong et al., 2015). For this reason, this tool is highly useful for epidemiological research since it provides more detailed and precise information for the timely taking of control measures derived from the identification, classification, transmission dynamics, the origin of infection, and possible patterns of spread of outbreaks with bacteria such as Klebsiella pneumoniae and Pseudomonas aeruginosa that cause Health Care Associated Infections (IAAS), these bacteria have a high degree of physiological adaptability and high levels of resistance against numerous antimicrobial agents, which constitutes them as a pathological with a high incidence of morbidity and mortality (Saharman et al., 2019) (Moradigaravand et al., 2017). One of the most frequent uses of data from Whole Genome Sequencing (WGS) is bacterial molecular typing. Consecuently, several methods have been developed are uptoday mainly based on analyzes derived from Ribosomal Multilocus Sequence Typing (rMLST), Core genome Multilocus Sequence Typing (cgMLST), Whole genome multilocus sequence typing (wgMLST), core genome Single Nucleotide Polymorphism ( cgSNP), whole-genome single nucleotide polymorphism (wgSNP) and pangenome (Coll et al., 2020) (Anani et al., 2020). These methods vary in their resolution and suitability depending on the species. However, due to the varied number of helpful bioinformatics tools for typing, and the lack of consensus benchmarking tools, researchers may face difficulties in choosing the right tools for their activities. For this reason, it is necessary to perform an evaluation of the performance of the tools for typing, to inform the user about the best bioinformatics tools currently available that provide accurate and relevant information.MaestríaMagíster en BioinformáticaBioinformática funcional y estructuralxx, 77 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ingeniería - Maestría en BioinformáticaFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::576 - Genética y evoluciónKlebsiella pneumoniaePseudomonas aeruginosaTaxaSecuencia de ADNDNA sequencesSecuenciación completa del genomaKlebsiella pneumoniaePseudomonas aeruginosaTipificación molecular bacterianaHerramientas BioinformáticasBenchmarkingWhole genome sequencingKlebsiella pneumoniaePseudomonas aeruginosaBacterial molecular typingBioinformatics toolsBenchmarkingEvaluación de herramientas bioinformáticas útiles en la tipificación de Klebsiella pneumoniae y Pseudomonas aeruginosa a partir de datos de secuenciación de genomas completosEvaluation of Bioinformatic tools useful in the typing of Klebsiella pneumoniae and Pseudomonas aeruginosa from whole genome sequencing dataTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlikhan, N. 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