Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia
ilustraciones, diagramas
- Autores:
-
Hernández Páez, Alexandra
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84398
- Palabra clave:
- Klebsiella pneumoniae
Aislamiento de Pacientes
Resistencia antimicrobiana
Carbapenemasas
Clones
Secuenciación de genoma completo
Infecciones intrahospitalarias
COVID-19
Antimicrobial resistance
Carbapenemases
Clusters
Nosocomial infections
Whole genome sequencing
Klebsiella pneumoniae
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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oai:repositorio.unal.edu.co:unal/84398 |
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
| dc.title.spa.fl_str_mv |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| dc.title.translated.eng.fl_str_mv |
Comparative genomic analysis of clinical isolates of Carbapenemase-producing Klebsiella pneumoniae in patients with and without SARS-CoV2 in the city of Bogotá during the pandemic period |
| title |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| spellingShingle |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia Klebsiella pneumoniae Aislamiento de Pacientes Resistencia antimicrobiana Carbapenemasas Clones Secuenciación de genoma completo Infecciones intrahospitalarias COVID-19 Antimicrobial resistance Carbapenemases Clusters Nosocomial infections Whole genome sequencing Klebsiella pneumoniae |
| title_short |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| title_full |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| title_fullStr |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| title_full_unstemmed |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| title_sort |
Análisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemia |
| dc.creator.fl_str_mv |
Hernández Páez, Alexandra |
| dc.contributor.advisor.none.fl_str_mv |
Barreto Hernández, Emiliano Leal Castro, Aura Lucía |
| dc.contributor.author.none.fl_str_mv |
Hernández Páez, Alexandra |
| dc.contributor.researchgroup.spa.fl_str_mv |
Bioinformática Epidemiología molecular |
| dc.subject.decs.other.fl_str_mv |
Klebsiella pneumoniae |
| topic |
Klebsiella pneumoniae Aislamiento de Pacientes Resistencia antimicrobiana Carbapenemasas Clones Secuenciación de genoma completo Infecciones intrahospitalarias COVID-19 Antimicrobial resistance Carbapenemases Clusters Nosocomial infections Whole genome sequencing Klebsiella pneumoniae |
| dc.subject.decs.spa.fl_str_mv |
Aislamiento de Pacientes |
| dc.subject.proposal.spa.fl_str_mv |
Resistencia antimicrobiana Carbapenemasas Clones Secuenciación de genoma completo Infecciones intrahospitalarias |
| dc.subject.proposal.eng.fl_str_mv |
COVID-19 Antimicrobial resistance Carbapenemases Clusters Nosocomial infections Whole genome sequencing |
| dc.subject.proposal.other.fl_str_mv |
Klebsiella pneumoniae |
| description |
ilustraciones, diagramas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-08-01T18:05:42Z |
| dc.date.available.none.fl_str_mv |
2023-08-01T18:05:42Z |
| dc.date.issued.none.fl_str_mv |
2023-01-31 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84398 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84398 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Parte 1 [Tesis de Especialización, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/bitstream/handle/unal/62824/SergioA.RemolinaGrana dos.2017.pdf?sequence=1&isAllowed=y Rojas, L. J., Weinstock, G. M., De La Cadena, E., Diaz, L., Rios, R., Hanson, B. M., Brown, J. S., Vats, P., Phillips, D. S., Nguyen, H., Hujer, K. M., Correa, A., Adams, M. D., Perez, F., Sodergren, E., Narechania, A., Planet, P. J., Villegas, M. V., Bonomo, R. T. A., & Arias, C. A. (2018). An analysis of the epidemic of klebsiella pneumoniae carbapenemase-producing k. pneumoniae: Convergence of two evolutionary mechanisms creates the “perfect storm.” Journal of Infectious Diseases, 217(1), 82–92. https://doi.org/10.1093/infdis/jix524 Saavedra, S. Y., Bernal, J. F., Montilla-Escudero, E., Arévalo, S. A., Prada, D. A., Valencia, M. F., Moreno, J., Hidalgo, A. M., Garciá-Vega, Á. S., Abrudan, M., Argimón, S., Kekre, M., Underwood, A., Aanensen, D. 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Virus Research, 285. https://doi.org/https://doi.org/10.1016/j.virusres.2020.198005 |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Barreto Hernández, Emilianob7a2cae2c08b5d6a549e173576c6c82d600Leal Castro, Aura Lucíaf07a334eb72fd9cef067a37ca1553060600Hernández Páez, Alexandraa0d33acb109172d55ac392c7f172beb2BioinformáticaEpidemiología molecular2023-08-01T18:05:42Z2023-08-01T18:05:42Z2023-01-31https://repositorio.unal.edu.co/handle/unal/84398Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasDurante la pandemia de COVID-19 se detectaron en los pacientes afectados por SARS-CoV-2 K. pneumoniae resistentes a carbapenémicos, lo que conduce a desenlaces fatales y desafíos para la resistencia antimicrobiana. En nuestro estudio, realizado en un hospital durante un periodo de la pandemia, se obtuvo la comparación epidemiológica y genómica de dos grupos de CRKP: 14 y 25, aisladas respectivamente de 10 y 19 pacientes COVID-19 y sin COVID-19; resaltando que las primeras CRKP fueron más resistentes frente a β-lactámicos como cefoxitina, pero menos resistentes frente a trimetoprim/sulfametoxazol y ciprofloxacina que las segundas CRKP. Fueron detectados 75 genes en todos los aislamientos del estudio; se presentaron coproducciones de genes relacionados con carbapenemasas en algunos de los genomas, la Kp082 tenía: NDM-1, KPC-3, GES-2, OXA-2 una combinación no reportada en K. pneumoniae. Se observó la diversidad genética de los dos grupos de aislamientos del estudio, dada por la presencia de variedad de STs y el árbol filogenético basado en los SNPs del core, donde se evidenció que 43,6% de las CRKP pertenecientes a los dos grupos de estudio presentes en el clado IV se asignaron a ST1082. Además, reveló que los pacientes 7, 19 y 8 presentaban aislamientos distribuidos en diferentes clados y como el resistoma presente en las CRKP aun de un mismo clon era variable. Estos hallazgos resaltan la necesidad de dirigir acciones para mejorar continuamente los protocolos de prevención, control de infecciones y programas de administración de antimicrobianos a fin de frenar la expansión de CRKP. (Texto tomado de la fuente)During the COVID-19 pandemic, carbapenem-resistant K. pneumoniae were detected in SARS-CoV-2-affected patients, leading to fatal outcomes and challenges to antimicrobial resistance. In our study, carried out in a hospital during a period of the pandemic, the epidemiological and genomic comparison of two groups of CRKP was obtained: 14 and 25, isolated respectively from 10 and 19 COVID-19 and non-COVID-19 patients; highlighting that the first CRKPs were more resistant against β-lactams such as cefoxitin, but less resistant against trimethoprim/sulfamethoxazole and ciprofloxacin than the second CRKPs. 75 genes were detected in all the study isolates; co-productions of genes related to carbapenemases were present in some of the genomes, Kp082 had: NDM-1, KPC-3, GES-2, OXA-2, a combination not reported in K. pneumoniae. The genetic diversity of the two groups of isolates in the study was observed, given the presence of a variety of STs and the phylogenetic tree based on the SNPs of the core, where it was evidenced that 43.6% of the CRKPs belonging to the two groups of study present in clade IV were assigned to ST1082. In addition, it revealed that patients 7, 19 and 8 had isolates distributed in different clades and that the resistome present in the CRKP even from the same clone was variable. These findings highlight the need for targeted actions to continually improve infection prevention, control protocols, and antimicrobial stewardship programs to curb the spread of CRKP.MaestríaMagíster en Ciencias - MicrobiologíaEste es un estudio de corte trasversal, a partir del cual se seleccionaron aislamientos de K. pneumoniae resistentes a carbapenémicos (CRKP) de 29 pacientes, los cuales ingresaron entre el 02 de enero del 2020 y el 10 de julio del 2021, en un hospital de tercer nivel de la ciudad de Bogotá D.C. Los pacientes correspondían a dos grupos, un grupo conformado por pacientes que hubieran ingresado al hospital con diagnóstico clínico de COVID-19 y/o con una prueba de PCR o Ag positivo para SARS-CoV-2 o que hubieran desarrollado esta enfermedad de manera intrahospitalaria posterior a su admisión; mientras que el otro grupo comprendía aquellos pacientes negativos para COVID-19 desde el ingreso y hasta la recolección del aislamiento.Biología Molecular de Agentes Infecciososxvii, 81 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogota, ColombiaUniversidad Nacional de Colombia - Sede BogotáAnálisis genómico comparativo de aislamientos clínicos de Klebsiella pneumoniae productores de carbapenemasas en pacientes con y sin SARS-CoV2 de la ciudad de Bogotá durante el periodo de pandemiaComparative genomic analysis of clinical isolates of Carbapenemase-producing Klebsiella pneumoniae in patients with and without SARS-CoV2 in the city of Bogotá during the pandemic periodTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBogotáAdler, A., Hussein, O., Ben-david, D., Masarwa, S., Navon-venezia, S., Schwaber, M. J., Carmeli, Y., Setton, E., Golan, S., Brill, S., Lipkin, V., Frodin, E., Mendelson, G., Rave, R., Yehuda, N., Aizen, I., Kaganski, M., Gershkovich, P., Sasson, A., … Charish, L. (2015). Persistence of Klebsiella pneumoniae ST258 as the predominant clone of carbapenemase-producing Enterobacteriaceae in post-acute-care hospitals in Israel, 2008-13. Journal of Antimicrobial Chemotherapy, 70(1), 89–92. https://doi.org/10.1093/jac/dku33Alcock, B. P., Raphenya, A. R., Lau, T. T. Y., Tsang, K. K., Bouchard, M., Edalatmand, A., Huynh, W., Nguyen, A. L. V., Cheng, A. A., Liu, S., Min, S. Y., Miroshnichenko, A., Tran, H. K., Werfalli, R. E., Nasir, J. A., Oloni, M., Speicher, D. J., Florescu, A., Singh, B., … McArthur, A. G. (2020). CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Research, 48(D1), D517–D525. https://doi.org/10.1093/nar/gkz935Amarsy, R., Jacquier, H., Munier, A. L., Merimèche, M., Berçot, B., & Mégarbane, B. 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Virus Research, 285. https://doi.org/https://doi.org/10.1016/j.virusres.2020.198005Klebsiella pneumoniaeAislamiento de PacientesResistencia antimicrobianaCarbapenemasasClonesSecuenciación de genoma completoInfecciones intrahospitalariasCOVID-19Antimicrobial resistanceCarbapenemasesClustersNosocomial infectionsWhole genome sequencingKlebsiella pneumoniaeEstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84398/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL38291096.2023.pdf38291096.2023.pdfTesis de Maestría en Ciencias-Microbiologíaapplication/pdf3646086https://repositorio.unal.edu.co/bitstream/unal/84398/2/38291096.2023.pdfb6f2c638999a50a3cfa6a2529aa49483MD52THUMBNAIL38291096.2023.pdf.jpg38291096.2023.pdf.jpgGenerated 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