Dengue disease diagnosis: A puzzle to be solved
Dengue is an infection caused by dengue virus and is the most important arthropod transmitted viral disease in the world, causing near 100 million cases and 50 000 fatalities each year. Health authorities believe that these numbers will grow in coming years. In Colombia, almost 600 municipalities ar...
- Autores:
-
Castellanos, Jaime Eduardo
Coronel-Ruíz, Carolina
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2014
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/65379
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/65379
http://bdigital.unal.edu.co/66402/
- Palabra clave:
- 61 Ciencias médicas; Medicina / Medicine and health
Dengue
Diagnóstico
Flavivirus
Serología
Vigilancia
Dengue
diagnóstico
Flavivirus
serología
vigilancia en dengue
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Dengue disease diagnosis: A puzzle to be solved |
| title |
Dengue disease diagnosis: A puzzle to be solved |
| spellingShingle |
Dengue disease diagnosis: A puzzle to be solved 61 Ciencias médicas; Medicina / Medicine and health Dengue Diagnóstico Flavivirus Serología Vigilancia Dengue diagnóstico Flavivirus serología vigilancia en dengue |
| title_short |
Dengue disease diagnosis: A puzzle to be solved |
| title_full |
Dengue disease diagnosis: A puzzle to be solved |
| title_fullStr |
Dengue disease diagnosis: A puzzle to be solved |
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Dengue disease diagnosis: A puzzle to be solved |
| title_sort |
Dengue disease diagnosis: A puzzle to be solved |
| dc.creator.fl_str_mv |
Castellanos, Jaime Eduardo Coronel-Ruíz, Carolina |
| dc.contributor.author.spa.fl_str_mv |
Castellanos, Jaime Eduardo Coronel-Ruíz, Carolina |
| dc.subject.ddc.spa.fl_str_mv |
61 Ciencias médicas; Medicina / Medicine and health |
| topic |
61 Ciencias médicas; Medicina / Medicine and health Dengue Diagnóstico Flavivirus Serología Vigilancia Dengue diagnóstico Flavivirus serología vigilancia en dengue |
| dc.subject.proposal.spa.fl_str_mv |
Dengue Diagnóstico Flavivirus Serología Vigilancia Dengue diagnóstico Flavivirus serología vigilancia en dengue |
| description |
Dengue is an infection caused by dengue virus and is the most important arthropod transmitted viral disease in the world, causing near 100 million cases and 50 000 fatalities each year. Health authorities believe that these numbers will grow in coming years. In Colombia, almost 600 municipalities are in regions with Aedes aegypti circulation, and the presence of four dengue serotypes has been demonstrated. Despite the increasing knowledge about disease pathogenesis and the dengue virus, some technical or scientific difficulties with diagnosing dengue remain, negatively affecting both public health surveillance and the appropriate attention to patients in health settings and hospitals. This paper reviews the principles and developments of the current diagnostic techniques for dengue, pointing out the difficulties with making accurate dengue diagnoses and case confirmations in public health and specialized laboratories. The principles and limitations of MAC-ELISA, IgG serology, viral NS1 detection and viral isolation by cell culture are presented. In addition, the review of immunochromatography techniques (rapid diagnostic tests) that have been put forward to help the point-of-care diagnosis is proposed. This paper is intended to bring forward some points of view about the issues related to dengue diagnosis and contribute to improve the discussion surrounding the strategies and techniques needed for reducing the impact of the disease and favoring its control. |
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2014 |
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2014-10-01 |
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2019-07-03T00:15:41Z |
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2019-07-03T00:15:41Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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ISSN: 2357-3848 |
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https://repositorio.unal.edu.co/handle/unal/65379 |
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ISSN: 2357-3848 |
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spa |
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spa |
| dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/revfacmed/article/view/45593 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista de la Facultad de Medicina Revista de la Facultad de Medicina |
| dc.relation.references.spa.fl_str_mv |
Castellanos, Jaime Eduardo and Coronel-Ruíz, Carolina (2014) Dengue disease diagnosis: A puzzle to be solved. Revista de la Facultad de Medicina, 62 (4). pp. 617-629. ISSN 2357-3848 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Castellanos, Jaime Eduardod72ae37b-ab14-4680-8ff3-c7a2eb0ef706300Coronel-Ruíz, Carolina12364fc5-efb7-4942-8bbe-26317fc3fca13002019-07-03T00:15:41Z2019-07-03T00:15:41Z2014-10-01ISSN: 2357-3848https://repositorio.unal.edu.co/handle/unal/65379http://bdigital.unal.edu.co/66402/Dengue is an infection caused by dengue virus and is the most important arthropod transmitted viral disease in the world, causing near 100 million cases and 50 000 fatalities each year. Health authorities believe that these numbers will grow in coming years. In Colombia, almost 600 municipalities are in regions with Aedes aegypti circulation, and the presence of four dengue serotypes has been demonstrated. Despite the increasing knowledge about disease pathogenesis and the dengue virus, some technical or scientific difficulties with diagnosing dengue remain, negatively affecting both public health surveillance and the appropriate attention to patients in health settings and hospitals. This paper reviews the principles and developments of the current diagnostic techniques for dengue, pointing out the difficulties with making accurate dengue diagnoses and case confirmations in public health and specialized laboratories. The principles and limitations of MAC-ELISA, IgG serology, viral NS1 detection and viral isolation by cell culture are presented. In addition, the review of immunochromatography techniques (rapid diagnostic tests) that have been put forward to help the point-of-care diagnosis is proposed. This paper is intended to bring forward some points of view about the issues related to dengue diagnosis and contribute to improve the discussion surrounding the strategies and techniques needed for reducing the impact of the disease and favoring its control.La infección por el virus de dengue es la enfermedad viral transmitida por insectos más importante del mundo que genera grandes gastos a los sistemas de salud. En Colombia, en más de 600 municipios está presente el mosquito vector y se ha detectado la circulación de los cuatro serotipos del virus, haciendo previsible que la incidencia y prevalencia sigan aumentando. Aunque se han hecho grandes avances y desarrollos para entender la enfermedad, existen vacíos científicos y técnicos que dificultan su correcto diagnóstico y que debilitan tanto la vigilancia en salud pública como la correcta atención en las instituciones prestadoras de salud. En este documento se revisan los principios e interpretaciones de las herramientas de laboratorio disponibles para el diagnóstico de dengue, haciendo énfasis en las dificultades existentes para la correcta definición de los casos desde el laboratorio general y especializado. Se explican los fundamentos y limitaciones de las técnicas de ELISA de captura de anticuerpos IgM, serología para IgG, detección del antígeno viral NS1, aislamiento viral en cultivos celulares y las pruebas más recientes basadas en la inmunocromatografía –pruebas rápidas–. La revisión se propone, además de aportar a la discusión sobre las dificultades para el diagnóstico en dengue, ofrecer una idea actualizada sobre cómo abordar el diagnóstico tanto en los laboratorios de salud pública como en los laboratorios especializados y contribuir así a mejorar los estándares de atención de los pacientes, reducir el impacto de la enfermedad y favorecer su control.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Medicinahttps://revistas.unal.edu.co/index.php/revfacmed/article/view/45593Universidad Nacional de Colombia Revistas electrónicas UN Revista de la Facultad de MedicinaRevista de la Facultad de MedicinaCastellanos, Jaime Eduardo and Coronel-Ruíz, Carolina (2014) Dengue disease diagnosis: A puzzle to be solved. Revista de la Facultad de Medicina, 62 (4). pp. 617-629. ISSN 2357-384861 Ciencias médicas; Medicina / Medicine and healthDengueDiagnósticoFlavivirusSerologíaVigilanciaDenguediagnósticoFlavivirusserologíavigilancia en dengueDengue disease diagnosis: A puzzle to be solvedArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL45593-246774-1-PB.pdfapplication/pdf1046914https://repositorio.unal.edu.co/bitstream/unal/65379/1/45593-246774-1-PB.pdf14d7b8e58965a52135dfb6ec9a086265MD51THUMBNAIL45593-246774-1-PB.pdf.jpg45593-246774-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7911https://repositorio.unal.edu.co/bitstream/unal/65379/2/45593-246774-1-PB.pdf.jpgfd16fc2d5644a0ee963d2cac075e0314MD52unal/65379oai:repositorio.unal.edu.co:unal/653792023-05-19 23:46:48.83Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |