The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
Alginate is a linear polymer composed of b-1,4 linked mannuronic acid and its epimer, a-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii grow...
- Autores:
-
Trujillo Roldán, Mauricio Alberto
Monsalve Gil, John Fredy
Cuesta Álvarez, Angélica Maria
Valdez Cruz, Norma Adriana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/60611
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/60611
http://bdigital.unal.edu.co/58943/
- Palabra clave:
- 62 Ingeniería y operaciones afines / Engineering
alginates
Azotobacter vinelandii
viscosifying power
unconventional carbon sources
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Trujillo Roldán, Mauricio Albertobaf51709-5db8-4933-bdba-9a5d8d6d3081300Monsalve Gil, John Fredyaca09c98-ddfa-40fb-b227-4b654710e54b300Cuesta Álvarez, Angélica Maria13e7e4d7-d944-4fb4-acbc-6074b16856df300Valdez Cruz, Norma Adriana4c4e6853-c354-4550-b463-c9de360e58ea3002019-07-02T18:42:52Z2019-07-02T18:42:52Z2015-11-01ISSN: 2346-2183https://repositorio.unal.edu.co/handle/unal/60611http://bdigital.unal.edu.co/58943/Alginate is a linear polymer composed of b-1,4 linked mannuronic acid and its epimer, a-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii growth, alginate production, its mean molecular weight (MMW) and its viscosifying power. Starting with 20 g/L of sugars, the highest biomass concentration was obtained using deproteinized and hydrolyzed whey (6.67±0.72 g/L), and sugarcane juice (6.68±0.45 g/L). However, the maximum alginate production was achieved using sucrose (5.11±0.37 g/L), as well the highest alginate yield and specific productivity. Otherwise, the higher alginate MMW was obtained using sugarcane juice (1203±120 kDa), and the higher viscosifying power was obtained using deproteinized/ hydrolyzed whey (23.8±2.6 cps L/galg). This information suggests that it is possible to manipulate the productivity and molecular characteristics of alginates, as a function of the carbon source used. All this, together with the knowledge of the effects of environmental conditions will allow for high yields of high added value biopolymers.application/pdfspaUniversidad Nacional de Colombia (Sede Medellín). Facultad de Minas.https://revistas.unal.edu.co/index.php/dyna/article/view/44201Universidad Nacional de Colombia Revistas electrónicas UN DynaDynaTrujillo Roldán, Mauricio Alberto and Monsalve Gil, John Fredy and Cuesta Álvarez, Angélica Maria and Valdez Cruz, Norma Adriana (2015) The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures. DYNA, 82 (194). pp. 21-26. ISSN 2346-218362 Ingeniería y operaciones afines / EngineeringalginatesAzotobacter vinelandiiviscosifying powerunconventional carbon sourcesThe production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged culturesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL44201-277812-1-PB.pdfapplication/pdf1035163https://repositorio.unal.edu.co/bitstream/unal/60611/1/44201-277812-1-PB.pdf131a39a1b7d63c57a76ad21144dffe22MD51THUMBNAIL44201-277812-1-PB.pdf.jpg44201-277812-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg9261https://repositorio.unal.edu.co/bitstream/unal/60611/2/44201-277812-1-PB.pdf.jpgbd1f518881455beaab567679301c8f76MD52unal/60611oai:repositorio.unal.edu.co:unal/606112023-04-08 23:04:25.626Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
| dc.title.spa.fl_str_mv |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| title |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| spellingShingle |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures 62 Ingeniería y operaciones afines / Engineering alginates Azotobacter vinelandii viscosifying power unconventional carbon sources |
| title_short |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| title_full |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| title_fullStr |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| title_full_unstemmed |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| title_sort |
The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures |
| dc.creator.fl_str_mv |
Trujillo Roldán, Mauricio Alberto Monsalve Gil, John Fredy Cuesta Álvarez, Angélica Maria Valdez Cruz, Norma Adriana |
| dc.contributor.author.spa.fl_str_mv |
Trujillo Roldán, Mauricio Alberto Monsalve Gil, John Fredy Cuesta Álvarez, Angélica Maria Valdez Cruz, Norma Adriana |
| dc.subject.ddc.spa.fl_str_mv |
62 Ingeniería y operaciones afines / Engineering |
| topic |
62 Ingeniería y operaciones afines / Engineering alginates Azotobacter vinelandii viscosifying power unconventional carbon sources |
| dc.subject.proposal.spa.fl_str_mv |
alginates Azotobacter vinelandii viscosifying power unconventional carbon sources |
| description |
Alginate is a linear polymer composed of b-1,4 linked mannuronic acid and its epimer, a-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii growth, alginate production, its mean molecular weight (MMW) and its viscosifying power. Starting with 20 g/L of sugars, the highest biomass concentration was obtained using deproteinized and hydrolyzed whey (6.67±0.72 g/L), and sugarcane juice (6.68±0.45 g/L). However, the maximum alginate production was achieved using sucrose (5.11±0.37 g/L), as well the highest alginate yield and specific productivity. Otherwise, the higher alginate MMW was obtained using sugarcane juice (1203±120 kDa), and the higher viscosifying power was obtained using deproteinized/ hydrolyzed whey (23.8±2.6 cps L/galg). This information suggests that it is possible to manipulate the productivity and molecular characteristics of alginates, as a function of the carbon source used. All this, together with the knowledge of the effects of environmental conditions will allow for high yields of high added value biopolymers. |
| publishDate |
2015 |
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2015-11-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-02T18:42:52Z |
| dc.date.available.spa.fl_str_mv |
2019-07-02T18:42:52Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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ISSN: 2346-2183 |
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https://repositorio.unal.edu.co/handle/unal/60611 |
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http://bdigital.unal.edu.co/58943/ |
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ISSN: 2346-2183 |
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https://repositorio.unal.edu.co/handle/unal/60611 http://bdigital.unal.edu.co/58943/ |
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spa |
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spa |
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https://revistas.unal.edu.co/index.php/dyna/article/view/44201 |
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Universidad Nacional de Colombia Revistas electrónicas UN Dyna Dyna |
| dc.relation.references.spa.fl_str_mv |
Trujillo Roldán, Mauricio Alberto and Monsalve Gil, John Fredy and Cuesta Álvarez, Angélica Maria and Valdez Cruz, Norma Adriana (2015) The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures. DYNA, 82 (194). pp. 21-26. ISSN 2346-2183 |
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Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidad Nacional de Colombia (Sede Medellín). Facultad de Minas. |
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