The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures

Alginate is a linear polymer composed of b-1,4 linked mannuronic acid and its epimer, a-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii grow...

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Autores:
Trujillo Roldán, Mauricio Alberto
Monsalve Gil, John Fredy
Cuesta Álvarez, Angélica Maria
Valdez Cruz, Norma Adriana
Tipo de recurso:
Article of journal
Fecha de publicación:
2015
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/60611
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/60611
http://bdigital.unal.edu.co/58943/
Palabra clave:
62 Ingeniería y operaciones afines / Engineering
alginates
Azotobacter vinelandii
viscosifying power
unconventional carbon sources
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_c50be009d9138bdd8f4cd98bba6ff759
oai_identifier_str oai:repositorio.unal.edu.co:unal/60611
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Trujillo Roldán, Mauricio Albertobaf51709-5db8-4933-bdba-9a5d8d6d3081300Monsalve Gil, John Fredyaca09c98-ddfa-40fb-b227-4b654710e54b300Cuesta Álvarez, Angélica Maria13e7e4d7-d944-4fb4-acbc-6074b16856df300Valdez Cruz, Norma Adriana4c4e6853-c354-4550-b463-c9de360e58ea3002019-07-02T18:42:52Z2019-07-02T18:42:52Z2015-11-01ISSN: 2346-2183https://repositorio.unal.edu.co/handle/unal/60611http://bdigital.unal.edu.co/58943/Alginate is a linear polymer composed of b-1,4 linked mannuronic acid and its epimer, a-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii growth, alginate production, its mean molecular weight (MMW) and its viscosifying power. Starting with 20 g/L of sugars, the highest biomass concentration was obtained using deproteinized and hydrolyzed whey (6.67±0.72 g/L), and sugarcane juice (6.68±0.45 g/L). However, the maximum alginate production was achieved using sucrose (5.11±0.37 g/L), as well the highest alginate yield and specific productivity. Otherwise, the higher alginate MMW was obtained using sugarcane juice (1203±120 kDa), and the higher viscosifying power was obtained using deproteinized/ hydrolyzed whey (23.8±2.6 cps L/galg). This information suggests that it is possible to manipulate the productivity and molecular characteristics of alginates, as a function of the carbon source used. All this, together with the knowledge of the effects of environmental conditions will allow for high yields of high added value biopolymers.application/pdfspaUniversidad Nacional de Colombia (Sede Medellín). Facultad de Minas.https://revistas.unal.edu.co/index.php/dyna/article/view/44201Universidad Nacional de Colombia Revistas electrónicas UN DynaDynaTrujillo Roldán, Mauricio Alberto and Monsalve Gil, John Fredy and Cuesta Álvarez, Angélica Maria and Valdez Cruz, Norma Adriana (2015) The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures. DYNA, 82 (194). pp. 21-26. ISSN 2346-218362 Ingeniería y operaciones afines / EngineeringalginatesAzotobacter vinelandiiviscosifying powerunconventional carbon sourcesThe production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged culturesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL44201-277812-1-PB.pdfapplication/pdf1035163https://repositorio.unal.edu.co/bitstream/unal/60611/1/44201-277812-1-PB.pdf131a39a1b7d63c57a76ad21144dffe22MD51THUMBNAIL44201-277812-1-PB.pdf.jpg44201-277812-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg9261https://repositorio.unal.edu.co/bitstream/unal/60611/2/44201-277812-1-PB.pdf.jpgbd1f518881455beaab567679301c8f76MD52unal/60611oai:repositorio.unal.edu.co:unal/606112023-04-08 23:04:25.626Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
title The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
spellingShingle The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
62 Ingeniería y operaciones afines / Engineering
alginates
Azotobacter vinelandii
viscosifying power
unconventional carbon sources
title_short The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
title_full The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
title_fullStr The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
title_full_unstemmed The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
title_sort The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures
dc.creator.fl_str_mv Trujillo Roldán, Mauricio Alberto
Monsalve Gil, John Fredy
Cuesta Álvarez, Angélica Maria
Valdez Cruz, Norma Adriana
dc.contributor.author.spa.fl_str_mv Trujillo Roldán, Mauricio Alberto
Monsalve Gil, John Fredy
Cuesta Álvarez, Angélica Maria
Valdez Cruz, Norma Adriana
dc.subject.ddc.spa.fl_str_mv 62 Ingeniería y operaciones afines / Engineering
topic 62 Ingeniería y operaciones afines / Engineering
alginates
Azotobacter vinelandii
viscosifying power
unconventional carbon sources
dc.subject.proposal.spa.fl_str_mv alginates
Azotobacter vinelandii
viscosifying power
unconventional carbon sources
description Alginate is a linear polymer composed of b-1,4 linked mannuronic acid and its epimer, a-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii growth, alginate production, its mean molecular weight (MMW) and its viscosifying power. Starting with 20 g/L of sugars, the highest biomass concentration was obtained using deproteinized and hydrolyzed whey (6.67±0.72 g/L), and sugarcane juice (6.68±0.45 g/L). However, the maximum alginate production was achieved using sucrose (5.11±0.37 g/L), as well the highest alginate yield and specific productivity. Otherwise, the higher alginate MMW was obtained using sugarcane juice (1203±120 kDa), and the higher viscosifying power was obtained using deproteinized/ hydrolyzed whey (23.8±2.6 cps L/galg). This information suggests that it is possible to manipulate the productivity and molecular characteristics of alginates, as a function of the carbon source used. All this, together with the knowledge of the effects of environmental conditions will allow for high yields of high added value biopolymers.
publishDate 2015
dc.date.issued.spa.fl_str_mv 2015-11-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T18:42:52Z
dc.date.available.spa.fl_str_mv 2019-07-02T18:42:52Z
dc.type.spa.fl_str_mv Artículo de revista
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format http://purl.org/coar/resource_type/c_6501
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dc.identifier.issn.spa.fl_str_mv ISSN: 2346-2183
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/60611
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/58943/
identifier_str_mv ISSN: 2346-2183
url https://repositorio.unal.edu.co/handle/unal/60611
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/dyna/article/view/44201
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Dyna
Dyna
dc.relation.references.spa.fl_str_mv Trujillo Roldán, Mauricio Alberto and Monsalve Gil, John Fredy and Cuesta Álvarez, Angélica Maria and Valdez Cruz, Norma Adriana (2015) The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures. DYNA, 82 (194). pp. 21-26. ISSN 2346-2183
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
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dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
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eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia (Sede Medellín). Facultad de Minas.
institution Universidad Nacional de Colombia
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