Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
ilustraciones, diagramas, fotografías
- Autores:
-
Umaña Bautista, Stefany Rocio
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84382
- Palabra clave:
- 610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Botánica médica
Materia médica, vegetable
Materia medica, Vegetable
Plantas medicinales
Medicine, botanic
Medicinal plant
Physalis peruviana L.
Artritis
Antioxidante
Inmunomodulador
Antiartrítico
Erosión ósea
Pannus
Modelo murino de artritis
Arthritis
Antioxidant
Immunomodulator
Antiarthritic
Bone erosion
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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oai:repositorio.unal.edu.co:unal/84382 |
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UNACIONAL2 |
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Universidad Nacional de Colombia |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
dc.title.translated.eng.fl_str_mv |
Preclinical evaluation of the butanolic fraction of calyces of Physalis peruviana L. in a murine model of rheumatoid arthritis |
title |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
spellingShingle |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide 610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades Botánica médica Materia médica, vegetable Materia medica, Vegetable Plantas medicinales Medicine, botanic Medicinal plant Physalis peruviana L. Artritis Antioxidante Inmunomodulador Antiartrítico Erosión ósea Pannus Modelo murino de artritis Arthritis Antioxidant Immunomodulator Antiarthritic Bone erosion |
title_short |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
title_full |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
title_fullStr |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
title_full_unstemmed |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
title_sort |
Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide |
dc.creator.fl_str_mv |
Umaña Bautista, Stefany Rocio |
dc.contributor.advisor.none.fl_str_mv |
Ospina Giraldo, Luis Fernando |
dc.contributor.author.none.fl_str_mv |
Umaña Bautista, Stefany Rocio |
dc.contributor.researchgroup.spa.fl_str_mv |
Principios Bioactivos en Plantas Medicinales |
dc.contributor.orcid.spa.fl_str_mv |
Umaña Bautista, Stefany |
dc.contributor.cvlac.spa.fl_str_mv |
Umaña Bautista, Stefany |
dc.contributor.scopus.spa.fl_str_mv |
Umaña Bautista, Stefany |
dc.contributor.researchgate.spa.fl_str_mv |
Umaña Bautista, Stefany |
dc.contributor.googlescholar.spa.fl_str_mv |
Umaña Bautista, Stefany |
dc.subject.ddc.spa.fl_str_mv |
610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades |
topic |
610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades Botánica médica Materia médica, vegetable Materia medica, Vegetable Plantas medicinales Medicine, botanic Medicinal plant Physalis peruviana L. Artritis Antioxidante Inmunomodulador Antiartrítico Erosión ósea Pannus Modelo murino de artritis Arthritis Antioxidant Immunomodulator Antiarthritic Bone erosion |
dc.subject.lemb.spa.fl_str_mv |
Botánica médica Materia médica, vegetable Materia medica, Vegetable Plantas medicinales |
dc.subject.lemb.eng.fl_str_mv |
Medicine, botanic Medicinal plant |
dc.subject.proposal.spa.fl_str_mv |
Physalis peruviana L. Artritis Antioxidante Inmunomodulador Antiartrítico Erosión ósea Pannus Modelo murino de artritis |
dc.subject.proposal.eng.fl_str_mv |
Arthritis Antioxidant Immunomodulator Antiarthritic Bone erosion |
description |
ilustraciones, diagramas, fotografías |
publishDate |
2023 |
dc.date.accessioned.none.fl_str_mv |
2023-07-31T20:41:56Z |
dc.date.available.none.fl_str_mv |
2023-07-31T20:41:56Z |
dc.date.issued.none.fl_str_mv |
2023 |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
status_str |
acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84382 |
dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
url |
https://repositorio.unal.edu.co/handle/unal/84382 https://repositorio.unal.edu.co/ |
identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.references.spa.fl_str_mv |
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Retrieved from https://www.clinexprheumatol.org/abstract.asp?a=15504. Gonçalves, G., Soares, A., Correa, R., Barros, L., Haminiuk, C., Peralta, R., Ferreira, R., & Bracht, A. (2017). Merlot grape pomace hydroalcoholic extract improves the oxidative and inflammatory states of rats with adjuvant-induced arthritis. Journal of Functional Foods, 33, 408–418. Retrieved from https://doi.org/10.1016/J.JFF.2017.04.009. Grant, C. (1997). Methotrexate pulmonary toxicity. Rheumatic Disease Clinics of North America, 23(4), 917–937. Retrieved from https://doi.org/10.1016/S0889-857X(05)70366-5. Grassi, W., de Angelis, R., Lamanna, G., & Cervini, C. (1998). The clinical features of rheumatoid arthritis. European Journal of Radiology, 27(SUPPL. 1). Retrieved from https://doi.org/10.1016/S0720-048X(98)00038-2. Gul, A., Kunwar, B., Mazhar, M., Faizi, S., Ahmed, D., Shah, M., & Simjee, S. (2018). Rutin and rutin-conjugated gold nanoparticles ameliorate collagen-induced arthritis in rats through inhibition of NF-κB and iNOS activation. International Immunopharmacology, 59, 310–317. Retrieved from https://doi.org/10.1016/J.INTIMP.2018.04.017. Gupta, A., & Singh, S. (2014). Evaluation of anti-inflammatory effect of Withania somnifera root on collagen-induced arthritis in rats. Pharmaceutical Biology, 52:3, 308-320. Retrieved from Https://Doi.Org/10.3109/13880209.2013.835325. Hazes, J., Coulie, P., Geenen, V., Vermeire, S., Carbonnel, F., Louis, E., Masson, P., & De Keyser, F. (2011). Rheumatoid arthritis and pregnancy: evolution of disease activity and pathophysiological considerations for drug use. Rheumatology, 50(11), 1955–1968. Retrieved from https://doi.org/10.1093/rheumatology/ker302. He, P., Hu, Y., Huang, C., Wang, X., Zhang, H., Zhang, X., Dai, H., Wang, R., & Gao, Y. (2020). N-butanol extract of Gastrodia elata suppresses inflammatory responses in lipopolysaccharide-stimulated macrophages and complete freund’s adjuvant- (CFA-) induced arthritis rats via inhibition of MAPK signaling pathway. Evidence-Based Complementary and Alternative Medicine: vol. 2020, article ID 1658618, 11 pages. Retrieved from https://doi.org/10.1155/2020/1658618. Hernández, C., & Martinez, C. (2009). Uso de fármacos modificadores de la enfermedad en artritis reumatoide. Revista Terapeutica de Sistema Nacional de Salud, 33(4), 99–109. Hitchon, C., & El-Gabalawy, H. (2004). Oxidation in rheumatoid arthritis. Arthritis Research & Therapy, 6(6), 265. Retrieved from https://doi.org/10.1186/AR1447. Hussain, A., Aslam, B., Muhammad, F., Faisal, M., Kousar, S., Mushtaq, A., & Bari, M. (2021). Anti-arthritic activity of Ricinus communis L. and Withania somnifera L. extracts in adjuvant-induced arthritic rats via modulating inflammatory mediators and subsiding oxidative stress. 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Inmunopatología de la artritis reumatoide. EMC - Aparato Locomotor, 47(4), 1–10. Retrieved from https://doi.org/10.1016/S1286-935X(14)69312-6. Narvaez, J. (2016). Tratamiento de la artritis reumatoide. Med Clin, 28, 1–5. Retrieved from https://doi.org/10.1016/j.med.2017.02.011. Nilles, J., Weiss, J., & Theile, D. (2022). Crystal violet staining is a reliable alternative to bicinchoninic acid assay-based normalization. BioTechniques, 73(3), 131–135. Retrieved from https://doi.org/10.2144/BTN-2022-0064/ASSET/IMAGES/LARGE/FIGURE2.JPEG. Olivares, E., Hernández, D., Núñez, C., & Cabiedes, J. (2011). Proteínas citrulinadas en artritis reumatoide. Reumatol Clin, 7(1), 68–71. Retrieved from https://doi.org/10.1016/j.reuma.2009.09.010. Oyenihi, B., Ollewagen, T., Myburgh, K., Powrie, Y., & Smith, C. (2019). Redox status and muscle pathology in rheumatoid arthritis: Insights from various rat hindlimb muscles. Oxidative Medicine and Cellular Longevity, vol. 2019, Article ID 2484678, 11 pages Retrieved from https://doi.org/10.1155/2019/2484678. Phull, A., Nasir, B., Haq, I., & Kim, S. (2018). Oxidative stress, consequences and EOR mediated cellular signaling in rheumatoid arthritis. Chemico-Biological Interactions, 281, 121–136. Retrieved from https://doi.org/10.1016/J.CBI.2017.12.024. Rojas A. (2019). Implementación de un modelo de artritis en pequeños roedores de laboratorio para el estudio de sustancias con actividad antiinflamatoria. (Tesis de maestría). Universidad Nacional de Colombia. Retrieved from https://repositorio.unal.edu.co/bitstream/handle/unal/76819/21047279.2019.pdf?sequence=1&isAllowed=y. Sánchez, D., Hernández, R., Fernández, M., Valdés, R., Martínez, C., León, J., Martínez, S., & Villanueva, G. (2005). Producción de iNOS y nitración de proteínas en la aorta de ratas expuestas a ozono. Rev Sanid Milit, 59(4), 223–240. 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Rheumatoid arthritis. Nature Reviews Disease Primers, 4, 18–20. Retrieved from https://doi.org/10.1038/nrdp.2018.2. Toro, R., Aragón, D., Ospina, L., Ramos, F., & Castellanos, L. (2014). Phytochemical analysis, antioxidant and anti-inflammatory activity of calyces from Physalis peruviana. Natural Product Communications, 9(11), 1573–1575. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/25532284. Van Delft, M., & Huizinga, T. (2020). An overview of autoantibodies in rheumatoid arthritis. Journal of Autoimmunity, 110, 102392. Retrieved from https://doi.org/10.1016/J.JAUT.2019.102392. Van den Berg, W., Joosten, L., Kollias, G., & van de Loo, F. (1999). Role of tumour necrosis factor α in experimental arthritis: separate activity of interleukin 1β in chronicity and cartilage destruction. Ann Rheum Dis, 58(1), I40–I48. Retrieved from https://doi.org/10.1136/ard.58.2008.i40. Van den Berg, W, Joosten, L, & van de Loo, F. (1999). TNF and IL-1β are separate targets in chronic arthritis. Clin Exp Rheumatol, 17 (18), S105–S114. Retrieved from https://www.clinexprheumatol.org/article.asp?a=1997. Van Riel, P., Smolen, J., Emery, P., Kalden, J., Dougados, M., Strand, C., & Breedveld, F. (2004). Leflunomide: a manageable safety profile. Journal of Rheumatology Supplement, 71, 21–24. World Health Organization. (2004). The global burden of disease 2004 (Vol. 1). Retrieved from https://doi.org/10.1038/npp.2011.85. Xie, X., Li, H., Wang, Y., Wan, Z., Luo, S., Zhao, Z., Liu, J., Wu, X., Li, X., & Li, X. (2019). Therapeutic effects of gentiopicroside on adjuvant-induced arthritis by inhibiting inflammation and oxidative stress in rats. International Immunopharmacology, 76, 105840. Retrieved from https://doi.org/10.1016/J.INTIMP.2019.105840. Yalamati, P., Bhongir, A., Karra, M., & Beedu, S. (2015). Comparative analysis of urinary total proteins by bicinchoninic acid and pyrogallol red molybdate methods. Journal of Clinical and Diagnostic Research: JCDR, 9(8), BC01-4. Retrieved from https://doi.org/10.7860/JCDR/2015/13543.6313. Yu, R., Song, D., DuBois, D., Almon, R., & Jusko, W. (2020). Modeling combined anti-inflammatory effects of dexamethasone and tofacitinib in arthritic rats HHS public access. AAPS J, 21(5), 93. Retrieved from https://doi.org/10.1208/s12248-019-0362-6. Zhao, J., Liu, T., Xu, F., You, S., Li, C., & Gu, Z. (2016). Anti-arthritic effects of total flavonoids from Juniperus sabina on complete Freund’s adjuvant induced arthritis in rats. Pharmacognosy Magazine, 12(47), 178. Retrieved from https://doi.org/10.4103/0973-1296.186346. Zhao, T., Xie, Z., Xi, Y., Liu, L., Li, Z., & Qin, D. (2022). How to model rheumatoid arthritis in animals: from rodents to non-human primates. Frontiers in Immunology, 13, 2487. Retrieved from https://doi.org/10.3389/FIMMU.2022.887460/BIBTEX. |
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Facultad de Ciencias |
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Bogotá,Colombia |
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Universidad Nacional de Colombia - Sede Bogotá |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ospina Giraldo, Luis Fernandobfb3464e494e8bac88c6cb72a6754547Umaña Bautista, Stefany Rocioc35c00af8c5a9a3ae6ea900e59bcf839Principios Bioactivos en Plantas MedicinalesUmaña Bautista, StefanyUmaña Bautista, StefanyUmaña Bautista, StefanyUmaña Bautista, StefanyUmaña Bautista, Stefany2023-07-31T20:41:56Z2023-07-31T20:41:56Z2023https://repositorio.unal.edu.co/handle/unal/84382Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasLa medicina tradicional se ha enfocado en estudiar los beneficios terapéuticos de plantas de la familia solanácea. En el caso de la planta Physalis peruviana L. se han descrito sus propiedades antiinflamatorias, antioxidantes e inmunomoduladoras. Sin embargo, en la actualidad no se ha reportado su uso en modelos murinos de inflamación crónica en artritis. Objetivo: Evaluar el posible efecto antiartrítico de la fracción butanólica de los cálices de Physalis peruviana L. en ratas Wistar hembras con artritis inducida por adyuvante completo de Freund (ACF) y colágeno. Método: La fracción butanólica de los cálices de Physalis peruviana L. se obtuvo a partir de la percolación con etanol de material seco, al cual se le hizo fraccionamiento con diferentes solventes hasta obtener la fracción butanólica. Se utilizó un modelo de inducción de artritis por adyuvante de Freund (ACF) y colágeno en ratas Wistar, evidenciando un pico de máxima inflamación el día 21. Los individuos fueron tratados desde el día 21 hasta el día 36 con dexametasona como medicamento patrón (1 mg/Kg/día) y la fracción butanólica (100, 50, 25 mg/Kg). La artritis fue evaluada a través de medición volumétrica de miembros posteriores (articulación del maléolo, zona plantar e interfalángica), evaluación radiográfica y análisis histopatológico. El efecto de la fracción butanólica sobre la inflamación se evaluó midiendo los niveles de interleucina 1 beta (IL-1β) y factor de necrosis tumoral alfa (TNFα); mientras que TBARs, la capacidad antioxidante (FRAP) y superóxido dismutasa (SOD) fueron medidos para evaluar el efecto de la fracción butanólica sobre el estrés oxidativo. Resultados: La fracción butanólica de cálices de Physalis peruviana L. no presentó diferencias significativas en la disminución de inflamación en comparación con el grupo patrón. Ninguno de los parámetros bioquímicos presentó diferencias significativas entre los grupos de investigación. La artritis inducida por ACF y colágeno no presentó involución de acuerdo con el análisis histopatológico y radiográfico. Conclusión: La fracción butanólica de cálices de Physalis peruviana L. (100, 50, 25 mg/Kg/día/VO) no presentó actividad inmunomoduladora ni antioxidante. Palabras clave: Physalis peruviana L., artritis, antioxidante, inmunomodulador, antiartrítico, erosión ósea y pannus. (Texto tomado de la fuente)Traditional medicine has focused on studying the therapeutic benefits of plants from the Solanaceae family. In the case of the Physalis peruviana L plant, its anti-inflammatory, antioxidant and immunomodulation properties have been described. However, its use in models of chronic inflammation in induced arthritis in murine models has not been reported to date. Objective: To evaluate the possible antiarthritic effect of the butanolic fraction of the calyces of Physalis peruviana L. in female Wistar rats with arthritis induced by complete Freund's adjuvant (CFA) and collagen. Method: The butanolic fraction of the calyces of Physalis peruviana L. was obtained from the percolation with ethanol of dry material, which was fractionated with different solvents until obtaining the butanolic fraction. A Freund's adjuvant (FCA) and collagen induction model of arthritis was used in Wistar rats, evidencing a peak of maximum inflammation on day 21. Individuals were treated with dexamethasone as standard drug (1 mg/Kg/day) and the butanolic fraction. (100, 50, 25 mg/Kg) from day 21 to day 36. Arthritis was evaluated through volumetric measurement of hind limbs (malleolar joint, plantar and interphalangeal area), radiographic evaluation, and histopathological analysis. The effect of the butanolic fraction on inflammation was evaluated by measuring the levels of interleukin (IL-1β) and tumor necrosis factor (TNFα) while TBARs, antioxidant capacity (FRAP) and superoxide dismutase (SOD) were measured to assess the effect of the butanolic fraction on oxidative stress. Results: The butanolic fraction of calyces of Physalis peruviana L. does not present significant differences in the reduction of inflammation compared to the standard group. None of the biochemical parameters presented significant differences between the research groups. The arthritis induced by CFA and collagen does not present involution according to the histopathological and radiographic analysis. Conclusion: The butanolic fraction of calyces of Physalis peruviana L. (100, 50, 25 mg/Kg/day/VO) did not present immunomodulatory and antioxidant activity. Keywords: Physalis peruviana L., arthritis, antioxidant, immunomodulator, antiarthritic, bone erosion, pannus.MaestríaMagíster en Ciencias - FarmacologíaLa fracción butanólica de los cálices de Physalis peruviana L. se obtuvo a partir de la percolación con etanol de material seco, al cual se le hizo fraccionamiento con diferentes solventes hasta obtener la fracción butanólica. Se utilizó un modelo de inducción de artritis por adyuvante de Freund (ACF) y colágeno en ratas Wistar, evidenciando un pico de máxima inflamación el día 21. Los individuos fueron tratados desde el día 21 hasta el día 36 con dexametasona como medicamento patrón (1 mg/Kg/día) y la fracción butanólica (100, 50, 25 mg/Kg). La artritis fue evaluada a través de medición volumétrica de miembros posteriores (articulación del maléolo, zona plantar e interfalángica), evaluación radiográfica y análisis histopatológico. El efecto de la fracción butanólica sobre la inflamación se evaluó midiendo los niveles de interleucina 1 beta (IL-1β) y factor de necrosis tumoral alfa (TNFα); mientras que TBARs, la capacidad antioxidante (FRAP) y superóxido dismutasa (SOD) fueron medidos para evaluar el efecto de la fracción butanólica sobre el estrés oxidativo.Plantas con actividad antiinflamatoria130 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - FarmacologíaFacultad de CienciasBogotá,ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::615 - Farmacología y terapéutica610 - Medicina y salud::616 - EnfermedadesBotánica médicaMateria médica, vegetableMateria medica, VegetablePlantas medicinalesMedicine, botanicMedicinal plantPhysalis peruviana L.ArtritisAntioxidanteInmunomoduladorAntiartríticoErosión óseaPannusModelo murino de artritisArthritisAntioxidantImmunomodulatorAntiarthriticBone erosionEvaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoidePreclinical evaluation of the butanolic fraction of calyces of Physalis peruviana L. in a murine model of rheumatoid arthritisTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAfonso, V., Champy, R., Mitrovic, D., Collin, P., & Lomri, A. 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Retrieved from https://doi.org/10.3389/FIMMU.2022.887460/BIBTEX.Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoideBibliotecariosEstudiantesInvestigadoresMaestrosPadres y familiasPersonal de apoyo escolarPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84382/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1057582996.2023.pdf1057582996.2023.pdfTesis de Maestría en Ciencias - Farmacologíaapplication/pdf3668166https://repositorio.unal.edu.co/bitstream/unal/84382/3/1057582996.2023.pdfbcfeed42d3ec950d4a8f6c5a770bddf9MD53THUMBNAIL1057582996.2023.pdf.jpg1057582996.2023.pdf.jpgGenerated Thumbnailimage/jpeg5046https://repositorio.unal.edu.co/bitstream/unal/84382/4/1057582996.2023.pdf.jpga1e035b5c1f967bfb4d5a7c1bdb05682MD54unal/84382oai:repositorio.unal.edu.co:unal/843822023-08-13 23:03:55.907Repositorio Institucional Universidad Nacional de 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