Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide

ilustraciones, diagramas, fotografías

Autores:
Umaña Bautista, Stefany Rocio
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/84382
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/84382
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Botánica médica
Materia médica, vegetable
Materia medica, Vegetable
Plantas medicinales
Medicine, botanic
Medicinal plant
Physalis peruviana L.
Artritis
Antioxidante
Inmunomodulador
Antiartrítico
Erosión ósea
Pannus
Modelo murino de artritis
Arthritis
Antioxidant
Immunomodulator
Antiarthritic
Bone erosion
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_c1fcea6f1eca40728492bbf9b6a5268c
oai_identifier_str oai:repositorio.unal.edu.co:unal/84382
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
dc.title.translated.eng.fl_str_mv Preclinical evaluation of the butanolic fraction of calyces of Physalis peruviana L. in a murine model of rheumatoid arthritis
title Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
spellingShingle Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Botánica médica
Materia médica, vegetable
Materia medica, Vegetable
Plantas medicinales
Medicine, botanic
Medicinal plant
Physalis peruviana L.
Artritis
Antioxidante
Inmunomodulador
Antiartrítico
Erosión ósea
Pannus
Modelo murino de artritis
Arthritis
Antioxidant
Immunomodulator
Antiarthritic
Bone erosion
title_short Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
title_full Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
title_fullStr Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
title_full_unstemmed Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
title_sort Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide
dc.creator.fl_str_mv Umaña Bautista, Stefany Rocio
dc.contributor.advisor.none.fl_str_mv Ospina Giraldo, Luis Fernando
dc.contributor.author.none.fl_str_mv Umaña Bautista, Stefany Rocio
dc.contributor.researchgroup.spa.fl_str_mv Principios Bioactivos en Plantas Medicinales
dc.contributor.orcid.spa.fl_str_mv Umaña Bautista, Stefany
dc.contributor.cvlac.spa.fl_str_mv Umaña Bautista, Stefany
dc.contributor.scopus.spa.fl_str_mv Umaña Bautista, Stefany
dc.contributor.researchgate.spa.fl_str_mv Umaña Bautista, Stefany
dc.contributor.googlescholar.spa.fl_str_mv Umaña Bautista, Stefany
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
topic 610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Botánica médica
Materia médica, vegetable
Materia medica, Vegetable
Plantas medicinales
Medicine, botanic
Medicinal plant
Physalis peruviana L.
Artritis
Antioxidante
Inmunomodulador
Antiartrítico
Erosión ósea
Pannus
Modelo murino de artritis
Arthritis
Antioxidant
Immunomodulator
Antiarthritic
Bone erosion
dc.subject.lemb.spa.fl_str_mv Botánica médica
Materia médica, vegetable
Materia medica, Vegetable
Plantas medicinales
dc.subject.lemb.eng.fl_str_mv Medicine, botanic
Medicinal plant
dc.subject.proposal.spa.fl_str_mv Physalis peruviana L.
Artritis
Antioxidante
Inmunomodulador
Antiartrítico
Erosión ósea
Pannus
Modelo murino de artritis
dc.subject.proposal.eng.fl_str_mv Arthritis
Antioxidant
Immunomodulator
Antiarthritic
Bone erosion
description ilustraciones, diagramas, fotografías
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-07-31T20:41:56Z
dc.date.available.none.fl_str_mv 2023-07-31T20:41:56Z
dc.date.issued.none.fl_str_mv 2023
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/84382
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/84382
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ospina Giraldo, Luis Fernandobfb3464e494e8bac88c6cb72a6754547Umaña Bautista, Stefany Rocioc35c00af8c5a9a3ae6ea900e59bcf839Principios Bioactivos en Plantas MedicinalesUmaña Bautista, StefanyUmaña Bautista, StefanyUmaña Bautista, StefanyUmaña Bautista, StefanyUmaña Bautista, Stefany2023-07-31T20:41:56Z2023-07-31T20:41:56Z2023https://repositorio.unal.edu.co/handle/unal/84382Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasLa medicina tradicional se ha enfocado en estudiar los beneficios terapéuticos de plantas de la familia solanácea. En el caso de la planta Physalis peruviana L. se han descrito sus propiedades antiinflamatorias, antioxidantes e inmunomoduladoras. Sin embargo, en la actualidad no se ha reportado su uso en modelos murinos de inflamación crónica en artritis. Objetivo: Evaluar el posible efecto antiartrítico de la fracción butanólica de los cálices de Physalis peruviana L. en ratas Wistar hembras con artritis inducida por adyuvante completo de Freund (ACF) y colágeno. Método: La fracción butanólica de los cálices de Physalis peruviana L. se obtuvo a partir de la percolación con etanol de material seco, al cual se le hizo fraccionamiento con diferentes solventes hasta obtener la fracción butanólica. Se utilizó un modelo de inducción de artritis por adyuvante de Freund (ACF) y colágeno en ratas Wistar, evidenciando un pico de máxima inflamación el día 21. Los individuos fueron tratados desde el día 21 hasta el día 36 con dexametasona como medicamento patrón (1 mg/Kg/día) y la fracción butanólica (100, 50, 25 mg/Kg). La artritis fue evaluada a través de medición volumétrica de miembros posteriores (articulación del maléolo, zona plantar e interfalángica), evaluación radiográfica y análisis histopatológico. El efecto de la fracción butanólica sobre la inflamación se evaluó midiendo los niveles de interleucina 1 beta (IL-1β) y factor de necrosis tumoral alfa (TNFα); mientras que TBARs, la capacidad antioxidante (FRAP) y superóxido dismutasa (SOD) fueron medidos para evaluar el efecto de la fracción butanólica sobre el estrés oxidativo. Resultados: La fracción butanólica de cálices de Physalis peruviana L. no presentó diferencias significativas en la disminución de inflamación en comparación con el grupo patrón. Ninguno de los parámetros bioquímicos presentó diferencias significativas entre los grupos de investigación. La artritis inducida por ACF y colágeno no presentó involución de acuerdo con el análisis histopatológico y radiográfico. Conclusión: La fracción butanólica de cálices de Physalis peruviana L. (100, 50, 25 mg/Kg/día/VO) no presentó actividad inmunomoduladora ni antioxidante. Palabras clave: Physalis peruviana L., artritis, antioxidante, inmunomodulador, antiartrítico, erosión ósea y pannus. (Texto tomado de la fuente)Traditional medicine has focused on studying the therapeutic benefits of plants from the Solanaceae family. In the case of the Physalis peruviana L plant, its anti-inflammatory, antioxidant and immunomodulation properties have been described. However, its use in models of chronic inflammation in induced arthritis in murine models has not been reported to date. Objective: To evaluate the possible antiarthritic effect of the butanolic fraction of the calyces of Physalis peruviana L. in female Wistar rats with arthritis induced by complete Freund's adjuvant (CFA) and collagen. Method: The butanolic fraction of the calyces of Physalis peruviana L. was obtained from the percolation with ethanol of dry material, which was fractionated with different solvents until obtaining the butanolic fraction. A Freund's adjuvant (FCA) and collagen induction model of arthritis was used in Wistar rats, evidencing a peak of maximum inflammation on day 21. Individuals were treated with dexamethasone as standard drug (1 mg/Kg/day) and the butanolic fraction. (100, 50, 25 mg/Kg) from day 21 to day 36. Arthritis was evaluated through volumetric measurement of hind limbs (malleolar joint, plantar and interphalangeal area), radiographic evaluation, and histopathological analysis. The effect of the butanolic fraction on inflammation was evaluated by measuring the levels of interleukin (IL-1β) and tumor necrosis factor (TNFα) while TBARs, antioxidant capacity (FRAP) and superoxide dismutase (SOD) were measured to assess the effect of the butanolic fraction on oxidative stress. Results: The butanolic fraction of calyces of Physalis peruviana L. does not present significant differences in the reduction of inflammation compared to the standard group. None of the biochemical parameters presented significant differences between the research groups. The arthritis induced by CFA and collagen does not present involution according to the histopathological and radiographic analysis. Conclusion: The butanolic fraction of calyces of Physalis peruviana L. (100, 50, 25 mg/Kg/day/VO) did not present immunomodulatory and antioxidant activity. Keywords: Physalis peruviana L., arthritis, antioxidant, immunomodulator, antiarthritic, bone erosion, pannus.MaestríaMagíster en Ciencias - FarmacologíaLa fracción butanólica de los cálices de Physalis peruviana L. se obtuvo a partir de la percolación con etanol de material seco, al cual se le hizo fraccionamiento con diferentes solventes hasta obtener la fracción butanólica. Se utilizó un modelo de inducción de artritis por adyuvante de Freund (ACF) y colágeno en ratas Wistar, evidenciando un pico de máxima inflamación el día 21. Los individuos fueron tratados desde el día 21 hasta el día 36 con dexametasona como medicamento patrón (1 mg/Kg/día) y la fracción butanólica (100, 50, 25 mg/Kg). La artritis fue evaluada a través de medición volumétrica de miembros posteriores (articulación del maléolo, zona plantar e interfalángica), evaluación radiográfica y análisis histopatológico. El efecto de la fracción butanólica sobre la inflamación se evaluó midiendo los niveles de interleucina 1 beta (IL-1β) y factor de necrosis tumoral alfa (TNFα); mientras que TBARs, la capacidad antioxidante (FRAP) y superóxido dismutasa (SOD) fueron medidos para evaluar el efecto de la fracción butanólica sobre el estrés oxidativo.Plantas con actividad antiinflamatoria130 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - FarmacologíaFacultad de CienciasBogotá,ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::615 - Farmacología y terapéutica610 - Medicina y salud::616 - EnfermedadesBotánica médicaMateria médica, vegetableMateria medica, VegetablePlantas medicinalesMedicine, botanicMedicinal plantPhysalis peruviana L.ArtritisAntioxidanteInmunomoduladorAntiartríticoErosión óseaPannusModelo murino de artritisArthritisAntioxidantImmunomodulatorAntiarthriticBone erosionEvaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoidePreclinical evaluation of the butanolic fraction of calyces of Physalis peruviana L. in a murine model of rheumatoid arthritisTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAfonso, V., Champy, R., Mitrovic, D., Collin, P., & Lomri, A. 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Retrieved from https://www.clinexprheumatol.org/abstract.asp?a=15504.Gonçalves, G., Soares, A., Correa, R., Barros, L., Haminiuk, C., Peralta, R., Ferreira, R., & Bracht, A. (2017). Merlot grape pomace hydroalcoholic extract improves the oxidative and inflammatory states of rats with adjuvant-induced arthritis. Journal of Functional Foods, 33, 408–418. Retrieved from https://doi.org/10.1016/J.JFF.2017.04.009.Grant, C. (1997). Methotrexate pulmonary toxicity. Rheumatic Disease Clinics of North America, 23(4), 917–937. Retrieved from https://doi.org/10.1016/S0889-857X(05)70366-5.Grassi, W., de Angelis, R., Lamanna, G., & Cervini, C. (1998). The clinical features of rheumatoid arthritis. European Journal of Radiology, 27(SUPPL. 1). Retrieved from https://doi.org/10.1016/S0720-048X(98)00038-2.Gul, A., Kunwar, B., Mazhar, M., Faizi, S., Ahmed, D., Shah, M., & Simjee, S. (2018). Rutin and rutin-conjugated gold nanoparticles ameliorate collagen-induced arthritis in rats through inhibition of NF-κB and iNOS activation. International Immunopharmacology, 59, 310–317. Retrieved from https://doi.org/10.1016/J.INTIMP.2018.04.017.Gupta, A., & Singh, S. (2014). Evaluation of anti-inflammatory effect of Withania somnifera root on collagen-induced arthritis in rats. Pharmaceutical Biology, 52:3, 308-320. Retrieved from Https://Doi.Org/10.3109/13880209.2013.835325.Hazes, J., Coulie, P., Geenen, V., Vermeire, S., Carbonnel, F., Louis, E., Masson, P., & De Keyser, F. (2011). Rheumatoid arthritis and pregnancy: evolution of disease activity and pathophysiological considerations for drug use. Rheumatology, 50(11), 1955–1968. Retrieved from https://doi.org/10.1093/rheumatology/ker302.He, P., Hu, Y., Huang, C., Wang, X., Zhang, H., Zhang, X., Dai, H., Wang, R., & Gao, Y. (2020). 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Retrieved from https://doi.org/10.3389/FIMMU.2022.887460/BIBTEX.Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoideBibliotecariosEstudiantesInvestigadoresMaestrosPadres y familiasPersonal de apoyo escolarPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84382/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1057582996.2023.pdf1057582996.2023.pdfTesis de Maestría en Ciencias - Farmacologíaapplication/pdf3668166https://repositorio.unal.edu.co/bitstream/unal/84382/3/1057582996.2023.pdfbcfeed42d3ec950d4a8f6c5a770bddf9MD53THUMBNAIL1057582996.2023.pdf.jpg1057582996.2023.pdf.jpgGenerated Thumbnailimage/jpeg5046https://repositorio.unal.edu.co/bitstream/unal/84382/4/1057582996.2023.pdf.jpga1e035b5c1f967bfb4d5a7c1bdb05682MD54unal/84382oai:repositorio.unal.edu.co:unal/843822023-08-13 23:03:55.907Repositorio Institucional Universidad Nacional de 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