Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador
Tree tomato (Solanum betaceum Cav.) is an economically important fruit crop in Ecuador. The symptoms of higher virus-related incidence in plantations located in Pichincha and Tungurahua correspond to yellowing, mosaic, leaf deformation and blistering. This research carried out a diagnosis of the vir...
- Autores:
-
Espinoza, Diego
Viera, William
Debut, Alexis
Vásquez, Wilson
Ayala, Ligia
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/61397
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/61397
http://bdigital.unal.edu.co/60207/
- Palabra clave:
- 63 Agricultura y tecnologías relacionadas / Agriculture
Potyvirus
ToMV
leaf deformation
chlorosis.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Espinoza, Diegoe79323bd-732e-4145-9671-f54cd5c38aec300Viera, William32297cc2-9f4d-45e0-8b17-d62c458a1443300Debut, Alexisb265d7fc-8530-4f58-827a-3b91adbb1a83300Vásquez, Wilson3d7afc86-5c8a-442f-bf5d-a5a75cbe8ce6300Ayala, Ligiac5b37621-168e-4657-a7cd-6cd27b550f6b3002019-07-02T20:41:56Z2019-07-02T20:41:56Z2017-01-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/61397http://bdigital.unal.edu.co/60207/Tree tomato (Solanum betaceum Cav.) is an economically important fruit crop in Ecuador. The symptoms of higher virus-related incidence in plantations located in Pichincha and Tungurahua correspond to yellowing, mosaic, leaf deformation and blistering. This research carried out a diagnosis of the viruses present in these production areas using molecular techniques (RT-PCR) and electron microscopy. The identified viruses belonged to the genera Potyvirus (Potato virus Y; PVY), Polerovirus (Potato leaf roll virus; PLRV) and Tobamovirus (Tomato mosaic virus; ToMV), constituting a viral complex of at least two of the three viruses found. The symptomatology related to PVY was spots with tan or oily appearance and leaf deformation; in addition to these symptoms, yellowing and chlorosis were related to PLRV. Foliar blistering was related to the occurrence of the three viruses. The results indicated the presence of the three viruses in Tumbaco (Pichincha) and two (PLRV and PVY) in Pelileo (Tungurahua). PLRV was found in the 100% of the total samples, PVY in 50% of the Pelileo’s samples and ToMV only in Tumbaco. ToMV virus has been detected for the first time infecting tree tomato in Ecuador.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/agrocol/article/view/60960Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaEspinoza, Diego and Viera, William and Debut, Alexis and Vásquez, Wilson and Ayala, Ligia (2017) Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador. Agronomía Colombiana, 35 (1). pp. 35-43. ISSN 2357-373263 Agricultura y tecnologías relacionadas / AgriculturePotyvirusToMVleaf deformationchlorosis.Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of EcuadorArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL60960-334767-1-PB.pdfapplication/pdf4949688https://repositorio.unal.edu.co/bitstream/unal/61397/1/60960-334767-1-PB.pdf81b8b2405514fe959c4cba5ec76a8378MD51THUMBNAIL60960-334767-1-PB.pdf.jpg60960-334767-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8845https://repositorio.unal.edu.co/bitstream/unal/61397/2/60960-334767-1-PB.pdf.jpgb1ae65adc5de954c365e4164ae093e12MD52unal/61397oai:repositorio.unal.edu.co:unal/613972024-04-18 23:51:09.06Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
| dc.title.spa.fl_str_mv |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| spellingShingle |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador 63 Agricultura y tecnologías relacionadas / Agriculture Potyvirus ToMV leaf deformation chlorosis. |
| title_short |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_full |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_fullStr |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_full_unstemmed |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_sort |
Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| dc.creator.fl_str_mv |
Espinoza, Diego Viera, William Debut, Alexis Vásquez, Wilson Ayala, Ligia |
| dc.contributor.author.spa.fl_str_mv |
Espinoza, Diego Viera, William Debut, Alexis Vásquez, Wilson Ayala, Ligia |
| dc.subject.ddc.spa.fl_str_mv |
63 Agricultura y tecnologías relacionadas / Agriculture |
| topic |
63 Agricultura y tecnologías relacionadas / Agriculture Potyvirus ToMV leaf deformation chlorosis. |
| dc.subject.proposal.spa.fl_str_mv |
Potyvirus ToMV leaf deformation chlorosis. |
| description |
Tree tomato (Solanum betaceum Cav.) is an economically important fruit crop in Ecuador. The symptoms of higher virus-related incidence in plantations located in Pichincha and Tungurahua correspond to yellowing, mosaic, leaf deformation and blistering. This research carried out a diagnosis of the viruses present in these production areas using molecular techniques (RT-PCR) and electron microscopy. The identified viruses belonged to the genera Potyvirus (Potato virus Y; PVY), Polerovirus (Potato leaf roll virus; PLRV) and Tobamovirus (Tomato mosaic virus; ToMV), constituting a viral complex of at least two of the three viruses found. The symptomatology related to PVY was spots with tan or oily appearance and leaf deformation; in addition to these symptoms, yellowing and chlorosis were related to PLRV. Foliar blistering was related to the occurrence of the three viruses. The results indicated the presence of the three viruses in Tumbaco (Pichincha) and two (PLRV and PVY) in Pelileo (Tungurahua). PLRV was found in the 100% of the total samples, PVY in 50% of the Pelileo’s samples and ToMV only in Tumbaco. ToMV virus has been detected for the first time infecting tree tomato in Ecuador. |
| publishDate |
2017 |
| dc.date.issued.spa.fl_str_mv |
2017-01-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-02T20:41:56Z |
| dc.date.available.spa.fl_str_mv |
2019-07-02T20:41:56Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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publishedVersion |
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ISSN: 2357-3732 |
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https://repositorio.unal.edu.co/handle/unal/61397 |
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http://bdigital.unal.edu.co/60207/ |
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ISSN: 2357-3732 |
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https://repositorio.unal.edu.co/handle/unal/61397 http://bdigital.unal.edu.co/60207/ |
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spa |
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spa |
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https://revistas.unal.edu.co/index.php/agrocol/article/view/60960 |
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Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
| dc.relation.references.spa.fl_str_mv |
Espinoza, Diego and Viera, William and Debut, Alexis and Vásquez, Wilson and Ayala, Ligia (2017) Virus diagnosis in tree tomato (iSolanum betaceum/i Cav.) by RT-PCR and transmission electron microscopy in Pichincha and Tungurahua Provinces of Ecuador. Agronomía Colombiana, 35 (1). pp. 35-43. ISSN 2357-3732 |
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Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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