Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
Abstract. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease in all regions where cassava is cultivated. To generate broad spectrum and durable CBB resistance it is necessary pyramiding several R genes. We have identified two candidate resistance...
- Autores:
-
Díaz Tatis, Paula Alejandra
- Tipo de recurso:
- Doctoral thesis
- Fecha de publicación:
- 2016
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/58098
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/58098
http://bdigital.unal.edu.co/54660/
- Palabra clave:
- 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
63 Agricultura y tecnologías relacionadas / Agriculture
Cassava
Xanthomonas
Bacterial
Blight
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López Carrascal, Camilo ErnestoBernal Giraldo, Adriana Jimena (Thesis advisor)4a80774f-690a-44d4-a5a8-1b3550988bbf-1Díaz Tatis, Paula Alejandra31c871f1-6547-4f6e-841a-357a7d088fd73002019-07-02T13:40:03Z2019-07-02T13:40:03Z2016-10-14https://repositorio.unal.edu.co/handle/unal/58098http://bdigital.unal.edu.co/54660/Abstract. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease in all regions where cassava is cultivated. To generate broad spectrum and durable CBB resistance it is necessary pyramiding several R genes. We have identified two candidate resistance genes, named RXam1 and RXam2 which colocalize with QTLs associated to resistance against Xam. RXam1 codes for a RLK (Receptor-Like Kinase) protein. We generated several transgenic cassava lines overexpressing RXam1. In vitro plants of three lines showed reduced symptoms and reduced bacterial growth after Xam infection with strain CIO136 compared to empty vector transgenic plants. The second cassava gene, RXam2, codes for a NB-LRR protein. Using RNAi we generated RXam2-silenced transgenic plants, which were more susceptible to several Xam strains than non-transformed plants. On the other hand, plants overexpressing RXam2 showed reduced symptoms to Xam strains. These data collectively suggest that RXam2 is a resistance gene against Xam. In addition, an autoactive version mutated in the MHD motif (NB domain) of RXam2 was generated through site directed mutagenesis and was able to generate a Hypersensitive Response (HR) by transient agroinfiltration in cassava and tobacco leaves. The autoactive version of RXam2 was cloned under a TALE1Xam-inducible promoter and transient expression in tobacco showed a strong HR when co-infiltrated with a plasmid containing the TALE1Xam gene. Several independent transgenic stable lines are being evaluated to assess TALE1Xam inducibility. Finally, several cassava transgenic plants overexpressing Bs2 from pepper were obtained and showed constitutive, typical immune responses.Doctoradoapplication/pdfspaUniversidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Departamento de Biología BiologíaBiologíaDíaz Tatis, Paula Alejandra (2016) Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB). Doctorado thesis, Universidad Nacional de Colombia-Sede Bogotá.57 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / Plants63 Agricultura y tecnologías relacionadas / AgricultureCassavaXanthomonasBacterialBlightTransference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)Trabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDORIGINAL55306965.2016.pdfapplication/pdf17722717https://repositorio.unal.edu.co/bitstream/unal/58098/1/55306965.2016.pdf83490529055688c6e32404d4fcaaebb9MD51THUMBNAIL55306965.2016.pdf.jpg55306965.2016.pdf.jpgGenerated Thumbnailimage/jpeg4581https://repositorio.unal.edu.co/bitstream/unal/58098/2/55306965.2016.pdf.jpg06d4db94b9c3fd27202c72176b947a31MD52unal/58098oai:repositorio.unal.edu.co:unal/580982023-03-26 23:05:26.518Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
title |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
spellingShingle |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) 57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants 63 Agricultura y tecnologías relacionadas / Agriculture Cassava Xanthomonas Bacterial Blight |
title_short |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
title_full |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
title_fullStr |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
title_full_unstemmed |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
title_sort |
Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB) |
dc.creator.fl_str_mv |
Díaz Tatis, Paula Alejandra |
dc.contributor.advisor.spa.fl_str_mv |
Bernal Giraldo, Adriana Jimena (Thesis advisor) |
dc.contributor.author.spa.fl_str_mv |
Díaz Tatis, Paula Alejandra |
dc.contributor.spa.fl_str_mv |
López Carrascal, Camilo Ernesto |
dc.subject.ddc.spa.fl_str_mv |
57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants 63 Agricultura y tecnologías relacionadas / Agriculture |
topic |
57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants 63 Agricultura y tecnologías relacionadas / Agriculture Cassava Xanthomonas Bacterial Blight |
dc.subject.proposal.spa.fl_str_mv |
Cassava Xanthomonas Bacterial Blight |
description |
Abstract. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease in all regions where cassava is cultivated. To generate broad spectrum and durable CBB resistance it is necessary pyramiding several R genes. We have identified two candidate resistance genes, named RXam1 and RXam2 which colocalize with QTLs associated to resistance against Xam. RXam1 codes for a RLK (Receptor-Like Kinase) protein. We generated several transgenic cassava lines overexpressing RXam1. In vitro plants of three lines showed reduced symptoms and reduced bacterial growth after Xam infection with strain CIO136 compared to empty vector transgenic plants. The second cassava gene, RXam2, codes for a NB-LRR protein. Using RNAi we generated RXam2-silenced transgenic plants, which were more susceptible to several Xam strains than non-transformed plants. On the other hand, plants overexpressing RXam2 showed reduced symptoms to Xam strains. These data collectively suggest that RXam2 is a resistance gene against Xam. In addition, an autoactive version mutated in the MHD motif (NB domain) of RXam2 was generated through site directed mutagenesis and was able to generate a Hypersensitive Response (HR) by transient agroinfiltration in cassava and tobacco leaves. The autoactive version of RXam2 was cloned under a TALE1Xam-inducible promoter and transient expression in tobacco showed a strong HR when co-infiltrated with a plasmid containing the TALE1Xam gene. Several independent transgenic stable lines are being evaluated to assess TALE1Xam inducibility. Finally, several cassava transgenic plants overexpressing Bs2 from pepper were obtained and showed constitutive, typical immune responses. |
publishDate |
2016 |
dc.date.issued.spa.fl_str_mv |
2016-10-14 |
dc.date.accessioned.spa.fl_str_mv |
2019-07-02T13:40:03Z |
dc.date.available.spa.fl_str_mv |
2019-07-02T13:40:03Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Doctorado |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_db06 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TD |
format |
http://purl.org/coar/resource_type/c_db06 |
status_str |
acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/58098 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/54660/ |
url |
https://repositorio.unal.edu.co/handle/unal/58098 http://bdigital.unal.edu.co/54660/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Departamento de Biología Biología Biología |
dc.relation.references.spa.fl_str_mv |
Díaz Tatis, Paula Alejandra (2016) Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB). Doctorado thesis, Universidad Nacional de Colombia-Sede Bogotá. |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
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application/pdf |
institution |
Universidad Nacional de Colombia |
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