Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)

Abstract. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease in all regions where cassava is cultivated. To generate broad spectrum and durable CBB resistance it is necessary pyramiding several R genes. We have identified two candidate resistance...

Full description

Autores:
Díaz Tatis, Paula Alejandra
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/58098
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/58098
http://bdigital.unal.edu.co/54660/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
63 Agricultura y tecnologías relacionadas / Agriculture
Cassava
Xanthomonas
Bacterial
Blight
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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oai_identifier_str oai:repositorio.unal.edu.co:unal/58098
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repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López Carrascal, Camilo ErnestoBernal Giraldo, Adriana Jimena (Thesis advisor)4a80774f-690a-44d4-a5a8-1b3550988bbf-1Díaz Tatis, Paula Alejandra31c871f1-6547-4f6e-841a-357a7d088fd73002019-07-02T13:40:03Z2019-07-02T13:40:03Z2016-10-14https://repositorio.unal.edu.co/handle/unal/58098http://bdigital.unal.edu.co/54660/Abstract. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease in all regions where cassava is cultivated. To generate broad spectrum and durable CBB resistance it is necessary pyramiding several R genes. We have identified two candidate resistance genes, named RXam1 and RXam2 which colocalize with QTLs associated to resistance against Xam. RXam1 codes for a RLK (Receptor-Like Kinase) protein. We generated several transgenic cassava lines overexpressing RXam1. In vitro plants of three lines showed reduced symptoms and reduced bacterial growth after Xam infection with strain CIO136 compared to empty vector transgenic plants. The second cassava gene, RXam2, codes for a NB-LRR protein. Using RNAi we generated RXam2-silenced transgenic plants, which were more susceptible to several Xam strains than non-transformed plants. On the other hand, plants overexpressing RXam2 showed reduced symptoms to Xam strains. These data collectively suggest that RXam2 is a resistance gene against Xam. In addition, an autoactive version mutated in the MHD motif (NB domain) of RXam2 was generated through site directed mutagenesis and was able to generate a Hypersensitive Response (HR) by transient agroinfiltration in cassava and tobacco leaves. The autoactive version of RXam2 was cloned under a TALE1Xam-inducible promoter and transient expression in tobacco showed a strong HR when co-infiltrated with a plasmid containing the TALE1Xam gene. Several independent transgenic stable lines are being evaluated to assess TALE1Xam inducibility. Finally, several cassava transgenic plants overexpressing Bs2 from pepper were obtained and showed constitutive, typical immune responses.Doctoradoapplication/pdfspaUniversidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Departamento de Biología BiologíaBiologíaDíaz Tatis, Paula Alejandra (2016) Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB). Doctorado thesis, Universidad Nacional de Colombia-Sede Bogotá.57 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / Plants63 Agricultura y tecnologías relacionadas / AgricultureCassavaXanthomonasBacterialBlightTransference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)Trabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDORIGINAL55306965.2016.pdfapplication/pdf17722717https://repositorio.unal.edu.co/bitstream/unal/58098/1/55306965.2016.pdf83490529055688c6e32404d4fcaaebb9MD51THUMBNAIL55306965.2016.pdf.jpg55306965.2016.pdf.jpgGenerated Thumbnailimage/jpeg4581https://repositorio.unal.edu.co/bitstream/unal/58098/2/55306965.2016.pdf.jpg06d4db94b9c3fd27202c72176b947a31MD52unal/58098oai:repositorio.unal.edu.co:unal/580982023-03-26 23:05:26.518Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
title Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
spellingShingle Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
63 Agricultura y tecnologías relacionadas / Agriculture
Cassava
Xanthomonas
Bacterial
Blight
title_short Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
title_full Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
title_fullStr Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
title_full_unstemmed Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
title_sort Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB)
dc.creator.fl_str_mv Díaz Tatis, Paula Alejandra
dc.contributor.advisor.spa.fl_str_mv Bernal Giraldo, Adriana Jimena (Thesis advisor)
dc.contributor.author.spa.fl_str_mv Díaz Tatis, Paula Alejandra
dc.contributor.spa.fl_str_mv López Carrascal, Camilo Ernesto
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
63 Agricultura y tecnologías relacionadas / Agriculture
topic 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
63 Agricultura y tecnologías relacionadas / Agriculture
Cassava
Xanthomonas
Bacterial
Blight
dc.subject.proposal.spa.fl_str_mv Cassava
Xanthomonas
Bacterial
Blight
description Abstract. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease in all regions where cassava is cultivated. To generate broad spectrum and durable CBB resistance it is necessary pyramiding several R genes. We have identified two candidate resistance genes, named RXam1 and RXam2 which colocalize with QTLs associated to resistance against Xam. RXam1 codes for a RLK (Receptor-Like Kinase) protein. We generated several transgenic cassava lines overexpressing RXam1. In vitro plants of three lines showed reduced symptoms and reduced bacterial growth after Xam infection with strain CIO136 compared to empty vector transgenic plants. The second cassava gene, RXam2, codes for a NB-LRR protein. Using RNAi we generated RXam2-silenced transgenic plants, which were more susceptible to several Xam strains than non-transformed plants. On the other hand, plants overexpressing RXam2 showed reduced symptoms to Xam strains. These data collectively suggest that RXam2 is a resistance gene against Xam. In addition, an autoactive version mutated in the MHD motif (NB domain) of RXam2 was generated through site directed mutagenesis and was able to generate a Hypersensitive Response (HR) by transient agroinfiltration in cassava and tobacco leaves. The autoactive version of RXam2 was cloned under a TALE1Xam-inducible promoter and transient expression in tobacco showed a strong HR when co-infiltrated with a plasmid containing the TALE1Xam gene. Several independent transgenic stable lines are being evaluated to assess TALE1Xam inducibility. Finally, several cassava transgenic plants overexpressing Bs2 from pepper were obtained and showed constitutive, typical immune responses.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-10-14
dc.date.accessioned.spa.fl_str_mv 2019-07-02T13:40:03Z
dc.date.available.spa.fl_str_mv 2019-07-02T13:40:03Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
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dc.type.content.spa.fl_str_mv Text
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status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/58098
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/54660/
url https://repositorio.unal.edu.co/handle/unal/58098
http://bdigital.unal.edu.co/54660/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Departamento de Biología Biología
Biología
dc.relation.references.spa.fl_str_mv Díaz Tatis, Paula Alejandra (2016) Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight (CBB). Doctorado thesis, Universidad Nacional de Colombia-Sede Bogotá.
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/58098/1/55306965.2016.pdf
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