Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6
ilustraciones, diagramas
- Autores:
-
Díaz Sana, Erika Vanessa
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/87057
- Palabra clave:
- 570 - Biología::572 - Bioquímica
610 - Medicina y salud::616 - Enfermedades
Péptidos
Neoplasias del Cuello Uterino
Neoplasias de la Mama
Integrinas
Peptides
Uterine Cervical Neoplasms
Breast Neoplasms
Integrins
Factor Nuclear κβ
Integrina αvβ6
Péptidos quiméricos
Muerte celular
Nuclear factor κβ
Integrin αvβ6
Chimeric peptides
Cell death
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| dc.title.translated.eng.fl_str_mv |
Effect of chimeric peptides on the proliferation and apoptosis of cancer cell lines and its correlation with the expression levels of the αVβ6 integrin |
| title |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| spellingShingle |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 570 - Biología::572 - Bioquímica 610 - Medicina y salud::616 - Enfermedades Péptidos Neoplasias del Cuello Uterino Neoplasias de la Mama Integrinas Peptides Uterine Cervical Neoplasms Breast Neoplasms Integrins Factor Nuclear κβ Integrina αvβ6 Péptidos quiméricos Muerte celular Nuclear factor κβ Integrin αvβ6 Chimeric peptides Cell death |
| title_short |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| title_full |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| title_fullStr |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| title_full_unstemmed |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| title_sort |
Efecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6 |
| dc.creator.fl_str_mv |
Díaz Sana, Erika Vanessa |
| dc.contributor.advisor.spa.fl_str_mv |
Urquiza Martínez, Mauricio |
| dc.contributor.author.spa.fl_str_mv |
Díaz Sana, Erika Vanessa |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Hormonas |
| dc.contributor.cvlac.spa.fl_str_mv |
https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000019953 |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::572 - Bioquímica 610 - Medicina y salud::616 - Enfermedades |
| topic |
570 - Biología::572 - Bioquímica 610 - Medicina y salud::616 - Enfermedades Péptidos Neoplasias del Cuello Uterino Neoplasias de la Mama Integrinas Peptides Uterine Cervical Neoplasms Breast Neoplasms Integrins Factor Nuclear κβ Integrina αvβ6 Péptidos quiméricos Muerte celular Nuclear factor κβ Integrin αvβ6 Chimeric peptides Cell death |
| dc.subject.decs.spa.fl_str_mv |
Péptidos Neoplasias del Cuello Uterino Neoplasias de la Mama Integrinas |
| dc.subject.decs.eng.fl_str_mv |
Peptides Uterine Cervical Neoplasms Breast Neoplasms Integrins |
| dc.subject.proposal.spa.fl_str_mv |
Factor Nuclear κβ Integrina αvβ6 Péptidos quiméricos Muerte celular |
| dc.subject.proposal.eng.fl_str_mv |
Nuclear factor κβ Integrin αvβ6 Chimeric peptides Cell death |
| description |
ilustraciones, diagramas |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-10-25T01:20:02Z |
| dc.date.available.none.fl_str_mv |
2024-10-25T01:20:02Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
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info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
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http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/87057 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/87057 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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Bireme |
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A New 4-phenyl-1,8-naphthyridine Derivative Affects Carcinoma Cell Proliferation by Impairing Cell Cycle Progression and Inducing Apoptosis. Anti-Cancer Agents in Medicinal Chemistry, 12(6), 653–662. https://doi.org/10.2174/187152012800617731 Caswell, P., & Norman, J. (2008). Endocytic transport of integrins during cell migration and invasion. Current Drug Targets, May, 257–263. https://doi.org/10.1016/j.tcb.2008.03.004 Cell line - ITGB6 - The Human Protein Atlas. (n.d.). Retrieved February 1, 2023, from https://www.proteinatlas.org/ENSG00000115221-ITGB6/cell+line Cell line - ITGB8 - The Human Protein Atlas. (n.d.). Retrieved February 1, 2023, from https://www.proteinatlas.org/ENSG00000105855-ITGB8/cell+line#brain_cancer Chen, J., Rowe, C. L., Jardetzky, T. S., & Longnecker, R. (2012). The KGD motif of Epstein-Barr virus gH/gL is bifunctional, orchestrating infection of B cells and epithelial cells. MBio, 3(1), 1–9. https://doi.org/10.1128/mBio.00290-11 Chesnokova, L. S., Nishimura, S. L., & Hutt-Fletcher, L. M. (2009). Fusion of epithelial cells by Epstein-Barr virus proteins is triggered by binding of viral glycoproteins gHgL to integrins v 6 or v 8. Proceedings of the National Academy of Sciences, 106(48), 20464–20469. https://doi.org/10.1073/pnas.0907508106 Chetoui, N., Gendron, S., Chamoux, E., & Aoudjit, F. (2006). Collagen type I-mediated activation of ERK/MAP Kinase is dependent on Ras, Raf-1 and protein phosphatase 2A in Jurkat T cells. Molecular Immunology, 43(10), 1687–1693. https://doi.org/10.1016/J.MOLIMM.2005.09.010 Collins, P., Grassia, G., Colleran, A., … P. K.-J. of B., & 2015. (n.d.). Mapping the interaction of B cell leukemia 3 (BCL-3) and nuclear factor κB (NF-κB) p50 identifies a BCL-3-mimetic anti-inflammatory peptide. ASBMB. Retrieved January 9, 2023, from https://www.jbc.org/article/S0021-9258(20)35065-1/abstract Collins, P., Kiely, P., Chemistry, R. C.-J. of B., & 2014, undefined. (n.d.). Inhibition of transcription by B cell Leukemia 3 (Bcl-3) protein requires interaction with nuclear factor κB (NF-κB) p50. ASBMB. Retrieved January 10, 2023, from https://www.jbc.org/article/S0021-9258(20)44522-3/abstract Dodagatta-Marri, E., Ma, H. Y., Liang, B., Li, J., Meyer, D. S., Chen, S. Y., Sun, K. H., Ren, X., Zivak, B., Rosenblum, M. D., Headley, M. B., Pinzas, L., Reed, N. I., del Cid, J. S., Hann, B. C., Yang, S., Giddabasappa, A., Noorbehesht, K., Yang, B., … Sheppard, D. (2021). Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Reports, 36(1). https://doi.org/10.1016/J.CELREP.2021.109309 Emonard, H., Dedieu, S., Dontenwill, M., Blandin, A.-F., Renner, G., Lehmann, M., Lelong-Rebel, I., & Martin, S. (2015). β1 Integrins as Therapeutic Targets to Disrupt Hallmarks of Cancer. 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Integrin α2β1 inhibits Fas-mediated apoptosis in T lymphocytes by protein phosphatase 2A-dependent activation of the MAPK/ERK pathway. Journal of Biological Chemistry, 278(49), 48633–48643. https://doi.org/10.1074/JBC.M305169200 Gong, Y., Fan, Z., Luo, G., Yang, C., Huang, Q., Fan, K., Cheng, H., Jin, K., Ni, Q., Yu, X., & Liu, C. (2019). The role of necroptosis in cancer biology and therapy. In Molecular Cancer (Vol. 18, Issue 1, pp. 1–17). BioMed Central Ltd. https://doi.org/10.1186/s12943-019-1029-8 Goswami, S. (2013). Importance of integrin receptors in the field of pharmaceutical & medical science. Advances in Biological Chemistry, 03(02), 224–252. https://doi.org/10.4236/abc.2013.32028 Gupta, S. C., Prasad, S., Reuter, S., Kannappan, R., Yadav, V. R., Ravindran, J., Hema, P. S., Chaturvedi, M. M., Nair, M., & Aggarwal, B. B. (2010). Modification of cysteine 179 of IκBα kinase by nimbolide leads to down-regulation of NF-κB-regulated cell survival and proliferative proteins and sensitization of tumor cells to chemotherapeutic agents. Journal of Biological Chemistry, 285(46), 35406–35417. https://doi.org/10.1074/jbc.M110.161984 Gupta, S. C., Sundaram, C., Reuter, S., & Aggarwal, B. B. (2010a). Inhibiting NF-κB activation by small molecules as a therapeutic strategy. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1799(10–12), 775–787. https://doi.org/10.1016/j.bbagrm.2010.05.004 Gupta, S. C., Sundaram, C., Reuter, S., & Aggarwal, B. B. (2010b). Inhibiting NF-κB activation by small molecules as a therapeutic strategy. In Biochimica et Biophysica Acta - Gene Regulatory Mechanisms (Vol. 1799, Issues 10–12, pp. 775–787). Elsevier. https://doi.org/10.1016/j.bbagrm.2010.05.004 Hernández-Luna, M. A., Díaz de León-Ortega, R., Hernández-Cueto, D. D., Gaxiola-Centeno, R., Castro-Luna, R., Martínez-Cristóbal, L., Huerta-Yépez, S., Luria-Pérez, R., Hernández-Luna, M. A., Díaz de León-Ortega, R., Hernández-Cueto, D. D., Gaxiola-Centeno, R., Castro-Luna, R., Martínez-Cristóbal, L., Huerta-Yépez, S., & Luria-Pérez, R. (2016). Bactofection of sequences encoding a Bax protein peptide chemosensitizes prostate cancer tumor cells. Boletín Médico Del Hospital Infantil de México, 73(6), 388–396. https://doi.org/10.1016/J.BMHIMX.2016.10.002 Karin, M., & Greten, F. R. (2005a). NF-κB: Linking inflammation and immunity to cancer development and progression. Nature Reviews Immunology, 5(10), 749–759. https://doi.org/10.1038/nri1703 Karin, M., & Greten, F. R. (2005b). NF-κB: linking inflammation and immunity to cancer development and progression. Nature Reviews Immunology, 5, 749. Kopatz, V., & Selzer, E. (2020). Quantitative and qualitative analysis of integrin subtype expression in melanocytes and melanoma cells. Journal of Receptors and Signal Transduction, 40(3), 237–245. https://doi.org/10.1080/10799893.2020.1727923 Kuroda, H., Tachikawa, M., Yagi, Y., Umetsu, M., Nurdin, A., Miyauchi, E., Watanabe, M., Uchida, Y., & Terasaki, T. (2019). Cluster of Differentiation 46 Is the Major Receptor in Human Blood-Brain Barrier Endothelial Cells for Uptake of Exosomes Derived from Brain-Metastatic Melanoma Cells (SK-Mel-28). Molecular Pharmaceutics, 16(1), 292–304. https://doi.org/10.1021/acs.molpharmaceut.8b00985 Lanzetti, L., & di Fiore, P. P. (2008). Endocytosis and cancer: An “Insider” network with dangerous liaisons. Traffic, 9(12), 2011–2021. https://doi.org/10.1111/j.1600-0854.2008.00816.x Legge, D. N., Chambers, A. C., Parker, C. T., Timms, P., Collard, T. J., & Williams, A. C. (2020). The role of B-Cell Lymphoma-3 (BCL-3) in enabling the hallmarks of cancer: implications for the treatment of colorectal carcinogenesis. 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E., Heino, J., Hakkinen, L., McKee, M. D., & Larjava, H. (2013). Critical role for v 6 integrin in enamel biomineralization. Journal of Cell Science, 126(3), 732–744. https://doi.org/10.1242/jcs.112599 Naci, D., el Azreq, M. A., Chetoui, N., Lauden, L., Sigaux, F., Charron, D., Al-Daccak, R., & Aoudjit, F. (2012). α2β1 integrin promotes chemoresistance against doxorubicin in cancer cells through extracellular signal-regulated kinase (ERK). The Journal of Biological Chemistry, 287(21), 17065–17076. https://doi.org/10.1074/JBC.M112.349365 Nieberler, M., Reuning, U., Reichart, F., Notni, J., Wester, H. J., Schwaiger, M., Weinmüller, M., Räder, A., Steiger, K., & Kessler, H. (2017). Exploring the role of RGD-recognizing integrins in cancer. Cancers, 9(9), 1–33. https://doi.org/10.3390/cancers9090116 Oeckinghaus, A., & Ghosh, S. (2009). The NF-kappaB family of transcription factors and its regulation. 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Oncogene, 3. https://doi.org/10.1038/s41388-018-0363-4 Ugarte-Alvarez, O., Muñoz-López, P., Moreno-Vargas, L. M., Prada-Gracia, D., Mateos-Chávez, A. A., Becerra-Báez, E. I., & Luria-Pérez, R. (2020). Cell-permeable BAK BH3 peptide induces chemosensitization of hematologic malignant cells. Journal of Oncology, 2020. https://doi.org/10.1155/2020/2679046 Uhlén, M., Fagerberg, L., Hallström, B. M., Lindskog, C., Oksvold, P., Mardinoglu, A., Sivertsson, Å., Kampf, C., Sjöstedt, E., Asplund, A., Olsson, I. M., Edlund, K., Lundberg, E., Navani, S., Szigyarto, C. A. K., Odeberg, J., Djureinovic, D., Takanen, J. O., Hober, S., … Pontén, F. (2015). Tissue-based map of the human proteome. Science, 347(6220). https://doi.org/10.1126/SCIENCE.1260419 Urquiza, M., Suarez, J., Lopez, R., Vega, E., Patino, H., Garcia, J., Patarroyo, M. A., Guzman, F., & Patarroyo, M. E. (2004). Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes. Biochemical and Biophysical Research Communications, 319(1), 221–229. https://doi.org/10.1016/j.bbrc.2004.04.177 Wen, S., Zhu, D., & Huang, P. (2013). Targeting cancer cell mitochondria as a therapeutic approach. In Future Medicinal Chemistry (Vol. 5, Issue 1, pp. 53–67). Future Science Ltd London, UK . https://doi.org/10.4155/fmc.12.190 Werner, J., Decarlo, C. A., Escott, N., Zehbe, I., & Ulanova, M. (2012). Expression of integrins and Toll-like receptors in cervical cancer: Effect of infectious agents. Innate Immunity, 18(1), 55–69. https://doi.org/10.1177/1753425910392934 Xie, Y., McElwee, K., Owen, G., … L. H.-J. of I., & 2012, U. (2012). Integrin β6-deficient mice show enhanced keratinocyte proliferation and retarded hair follicle regression after depilation. Journal of Investigative Dermatology, 132(3), 547–555. Ying, S., & Häcker, G. (2007). Apoptosis induced by direct triggering of mitochondrial apoptosis proceeds in the near-absence of some apoptotic markers. Apoptosis, 12(11), 2003–2011. https://doi.org/10.1007/s10495-007-0117-1 |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Urquiza Martínez, Mauricio6b6d659b9ea782b1d1486a0ca6f71fb8600Díaz Sana, Erika Vanessac4aca115f22a886d0d2742cbb0a54d37600Grupo de Investigación en Hormonashttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=00000199532024-10-25T01:20:02Z2024-10-25T01:20:02Z2023https://repositorio.unal.edu.co/handle/unal/87057Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasLa invasión celular, migración y metástasis en algunos tipos de tumores es promovida por la desregulación en la señalización del factor nuclear κβ y la sobre expresión de la integrina αvβ6. La presencia de esta integrina se correlaciona con malignidad de las lesiones, por lo que se considera un biomarcador y blanco terapéutico en células tumorales. El factor nuclear κβ (NF- κβ) regula la transcripción de genes involucrados en la respuesta inmune y respuestas celulares como adhesión, diferenciación y apoptosis. La activación inapropiada o exacerbada del NF- κβ está involucrada en diversos tipos de patologías como enfermedades inflamatorias crónicas, autoinmunes, y el desarrollo y progresión del cáncer, específicamente en carcinomas de seno, pulmón, boca, estómago, endometrio, páncreas, y ovario. En esta tesis reportamos que los péptidos quiméricos P63 y P68, sintetizados con motivos de unión la integrina αvβ6 y un motivo que bloquea la señalización del NF- κβ mediada por la proteína BCL-3. (1) Son capaces de unirse en mayor proporción a células de cáncer de cérvix, seno, leucemia linfoide aguda, melanoma y pulmón (HeLa, MCF-7, CCRF-CEM, Skmel 23 y A549 respectivamente) en comparación con líneas celulares no tumorales como HEK 293, fibroblastos y células mononucleares de sangre periférica (PBMCs) de un individuo sano. (2) También, modifican el potencial de membrana mitocondrial de manera dosis y tiempo dependiente y (3) promueven la expresión de marcadores de apoptosis en las células tumorales HeLa y MCF-7 pero no en células normales. Estos resultados, permiten conocer el funcionamiento de los péptidos a nivel biológico y molecular y arrojan información sobre su potencial para controlar procesos tumorales de una manera específica y bajo efecto citotóxico en células sanas. (Texto tomado de la fuente).Cell invasion, migration, and metastasis in some types of tumors is promoted by deregulation of nuclear factor κβ signaling and overexpression of integrin αvβ6. The presence of this integrin correlates with the malignancy of the lesions, which is why it is considered a biomarker and therapeutic target in tumor cells. Nuclear factor κβ (NF- κβ) regulates the transcription of genes involved in the immune response and cellular responses such as adhesion, differentiation, and apoptosis. Inappropriate or exacerbated activation of NF-κβ is involved in various types of pathologies such as chronic inflammatory and autoimmune diseases, and the development and progression of cancer, specifically in carcinomas of the breast, lung, mouth, stomach, endometrium, pancreas, and ovary. In this article we report that chimeric peptides P63 and P68, synthesized with integrin αvβ6 binding motifs and a motif that blocks NF-κβ signaling mediated by the BCL-3 protein. (1) They are able to bind to a higher proportion of cervical, breast, acute lymphoid leukemia, melanoma and lung cancer cells (HeLa, MCF-7, CCRF-CEM, Skmel 23 and A549 respectively) compared to non-tumor cell lines as HEK 293, fibroblasts and peripheral blood mononuclear cells (PBMCs) from a healthy individual. (2) Also, they modify the mitochondrial membrane potential in a dose- and time-dependent manner and (3) they promote the expression of apoptosis markers in HeLa and MCF-7 tumor cells but not in normal cells. These results allow us to know the functioning of the peptides at a biological and molecular level and shed information on their potential to control tumor processes in a specific way and with a low cytotoxic effect on healthy cells.MaestríaMagíster en Ciencias - Bioquímicaxii, 52 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - Bioquímica610 - Medicina y salud::616 - EnfermedadesPéptidosNeoplasias del Cuello UterinoNeoplasias de la MamaIntegrinasPeptidesUterine Cervical NeoplasmsBreast NeoplasmsIntegrinsFactor Nuclear κβIntegrina αvβ6Péptidos quiméricosMuerte celularNuclear factor κβIntegrin αvβ6Chimeric peptidesCell deathEfecto de péptidos quiméricos en la proliferación y apoptosis de líneas celulares de cáncer y su correlación con los niveles de expresión de la integrina αVβ6Effect of chimeric peptides on the proliferation and apoptosis of cancer cell lines and its correlation with the expression levels of the αVβ6 integrinTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBiremeAlbert, J. 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Apoptosis, 12(11), 2003–2011. https://doi.org/10.1007/s10495-007-0117-1InvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/87057/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1018448541.2022.pdf1018448541.2022.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf1455658https://repositorio.unal.edu.co/bitstream/unal/87057/2/1018448541.2022.pdf2e4f7ae5b5da2fffe60b0fcec0042fe2MD52THUMBNAIL1018448541.2022.pdf.jpg1018448541.2022.pdf.jpgGenerated Thumbnailimage/jpeg5690https://repositorio.unal.edu.co/bitstream/unal/87057/3/1018448541.2022.pdf.jpg9507a82687e9f48cf261303bbb076f11MD53unal/87057oai:repositorio.unal.edu.co:unal/870572024-10-25 00:06:29.574Repositorio Institucional Universidad Nacional de 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