Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico
ilustraciones, diagramas
- Autores:
-
Pachón Venegas, Carolina
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85357
- Palabra clave:
- 570 - Biología::571 - Fisiología y temas relacionados
Bacterias
Bacteria
Enzimas antioxidantes
Fisiología
Fotosistema II
Prolina
Sequía
Antioxidant enzymes
Physiology
Photosystem II
proline
drought
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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oai:repositorio.unal.edu.co:unal/85357 |
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| dc.title.translated.eng.fl_str_mv |
Effect of inoculation of plant growth-promoting bacteria on “Altoandina” oat (Avena sativa) under water deficit stress |
| title |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| spellingShingle |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico 570 - Biología::571 - Fisiología y temas relacionados Bacterias Bacteria Enzimas antioxidantes Fisiología Fotosistema II Prolina Sequía Antioxidant enzymes Physiology Photosystem II proline drought |
| title_short |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| title_full |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| title_fullStr |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| title_full_unstemmed |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| title_sort |
Efecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídrico |
| dc.creator.fl_str_mv |
Pachón Venegas, Carolina |
| dc.contributor.advisor.none.fl_str_mv |
Estrada Bonilla, Germán Andrés Álvarez Flórez, Fagua Virginia |
| dc.contributor.author.none.fl_str_mv |
Pachón Venegas, Carolina |
| dc.contributor.researchgroup.spa.fl_str_mv |
Fisiología del Estrés y Biodiversidad en Plantas y Microorganismos |
| dc.contributor.orcid.spa.fl_str_mv |
Pachón Venegas, Carolina [0000-0002-1560-1410] |
| dc.contributor.cvlac.spa.fl_str_mv |
Pachón Venegas, Carolina [https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001760577] |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::571 - Fisiología y temas relacionados |
| topic |
570 - Biología::571 - Fisiología y temas relacionados Bacterias Bacteria Enzimas antioxidantes Fisiología Fotosistema II Prolina Sequía Antioxidant enzymes Physiology Photosystem II proline drought |
| dc.subject.decs.none.fl_str_mv |
Bacterias Bacteria |
| dc.subject.proposal.spa.fl_str_mv |
Enzimas antioxidantes Fisiología Fotosistema II Prolina Sequía |
| dc.subject.proposal.eng.fl_str_mv |
Antioxidant enzymes Physiology Photosystem II proline drought |
| description |
ilustraciones, diagramas |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-01-18T15:08:24Z |
| dc.date.available.none.fl_str_mv |
2024-01-18T15:08:24Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
DataPaper Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85357 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/85357 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Estrada Bonilla, Germán Andrés714d3baacc98fc3b38b73b59433dbd41Álvarez Flórez, Fagua Virginia7d8f58906534abdd96e558c4bd6e5e35Pachón Venegas, Carolina8fc7f8151bae720927a32f51aa3b77bbFisiología del Estrés y Biodiversidad en Plantas y MicroorganismosPachón Venegas, Carolina [0000-0002-1560-1410]Pachón Venegas, Carolina [https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001760577]2024-01-18T15:08:24Z2024-01-18T15:08:24Z2023https://repositorio.unal.edu.co/handle/unal/85357Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasEl genotipo de avena Altoandina (Avena sativa AV25T) fue seleccionado y estudiado para su cultivo en las regiones del Altiplano Cundiboyacense y de Nariño, demostrando resistencia a la roya, rápido desarrollo y alto nivel nutricional. Sin embargo, se ha reportado una baja en la producción bajo condiciones de déficit hídrico, las cuales se deben estudiar a mayor profundidad. Se ha propuesto la utilización de bioestimulantes a base de las bacterias promotoras del crecimiento vegetal (PGPB) como una estrategia de mitigación del estrés en diferentes sistemas agrícolas, como una alternativa de respuesta rápida frente a eventos de cambio climático como la sequía. Por lo tanto, el objetivo de este trabajo fue evaluar a nivel fisiológico, bioquímico y de expresión el efecto de la inoculación de PGPB en avena Altoandina (Avena sativa) bajo condiciones controladas de estrés por déficit hídrico. Se realizaron dos experimentos bajo condiciones controladas: el primero con el objetivo de seleccionar entre 21 inoculaciones y co-inoculaciones las que presentaran mejor desempeño a nivel de crecimiento, estado hídrico y estado del fotosistema II (PSII) bajo condiciones de estrés severo (HRs < 20%; gs ≤ 80 mmol de H2O/m2s). Como resultado, se seleccionaron los tratamientos inoculados con Pseudomonas fluorescens N7, Bacillus amyloliquefaciens XT17 y las co-inoculaciones P. fluorescens N7 + B. amyloliquefaciens XT17 y Azospirillum brasilense D7 + Herbaspirillum sp. AP21. Para el segundo, se evaluó a nivel fisiológico, bioquímico y de expresión diferencial el efecto del estrés leve, severo y rehidratación en las plantas inoculadas y no inoculadas. Las plantas de avena Altoandina presentaron un buen desempeño durante el estrés hídrico, con una recuperación del RWC una vez se reestableció el riego, crecimiento radicular similar al control irrigado, producción de osmolitos protectores y no hubo fotoinhibición del PSII. Aunque los tratamientos presentaron valores similares al control estresado, se presentaron diferencias durante la rehidratación (P<0,05), donde hay mayor presencia de osmolitos, enzimas antioxidantes y eficiencia del fotosistema en plantas inoculadas; se destacan los tratamientos P. fluorescens N7 y su co-inoculación P. fluorescens N7 + B. amyloliquefaciens XT17. Adicionalmente, la inoculación de bacterias presenta un alivio de las condiciones de estrés leve relacionada con la expresión de genes de la ruta de producción de prolina (P5Cs1 y PDH1). Finalmente, avena Altoandina presenta un comportamiento de tipo tolerante al estrés por déficit hídrico, sin embargo, la inoculación de PGPB presenta un efecto de alivio al estrés durante la rehidratación, siendo potencial para la recuperación total de las plantas en estados más avanzados de su fenología. (Texto tomado de la fuente)The genotype "Altoandina" oat (Avena sativa AV25T) was previously selected and studied for cultivation in the High Tropic region of Colombia. This oat genotype has shown resistance to rust, rapid development, and high nutritional value, making it suitable for use as forage. However, a decrease in production has been observed under drought conditions, which requires further in-depth research. The use of bio-stimulants based on plant growth-promoting bacteria (PGPB) has been proposed as a potential tool to mitigate stress in various agricultural systems, offering a rapid response alternative to climate change events, such as intense drought. Hence, the main objective of this study was to assess the physiological, biochemical, and gene expression-level impacts of PGPB inoculation on "Altoandina" oat plants subjected to controlled water deficit stress conditions. Two separate assays were carried out under controlled conditions: the first aimed to identify the most effective inoculations and co-inoculations based on growth, water status, and photosystem II (PSII) performance under severe stress conditions (HRs < 20%; gs ≤ 80 mmol H2O/m2s). The selected treatments included those inoculated with Pseudomonas fluorescens N7, Bacillus amyloliquefaciens XT17, and the co-inoculations P. fluorescens N7 + B. amyloliquefaciens XT17 and Azospirillum brasilense D7 + Herbaspirillum sp. AP21. Subsequently, the second experiment involved assessing the physiological, biochemical, and differential expression effects of mild and severe stress, as well as rehydration, in both inoculated and non-inoculated plants. This comprehensive approach allowed us to gain valuable insights into the potential benefits of PGPB inoculation in mitigating water deficit stress in "Altoandina" oat plants. Under water stress conditions, the plants demonstrated performance, exhibiting a notable recovery of relative water content (RWC) once irrigation was restored, along with comparable root growth to the irrigated control. Although the treatments showed similar values to the stressed control, differences were observed during rehydration (P<0.05), with higher concentration of osmolytes, antioxidant enzymes, and photosystem efficiency in inoculated plants. Notably, the treatments involving P. fluorescens N7, particularly its co-inoculation with B. amyloliquefaciens XT17, displayed outstanding performance during the rehydration phase. Additionally, bacterial inoculation alleviated mild stress conditions, as indicated by the upregulation of genes involved in proline production (P5Cs1 and PDH1). Finally, this variety exhibited a stress-tolerant behavior under water deficit conditions; however, PGPB inoculation showed a stress relief effect during rehydration, with potential for complete recovery of plants in more advanced stages of their phenology.MaestríaMagister en Ciencias BiologíaFisología y bioquímica vegetalFisiología del estrésxiii, 60 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::571 - Fisiología y temas relacionadosBacteriasBacteriaEnzimas antioxidantesFisiologíaFotosistema IIProlinaSequíaAntioxidant enzymesPhysiologyPhotosystem IIprolinedroughtEfecto de la inoculación de bacterias promotoras del crecimiento en avena forrajera Altoandina (Avena sativa) bajo condiciones de estrés por déficit hídricoEffect of inoculation of plant growth-promoting bacteria on “Altoandina” oat (Avena sativa) under water deficit stressTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionDataPaperTexthttp://purl.org/redcol/resource_type/TMAbdelaal, K., AlKahtani, M., Attia, K., Hafez, Y., Király, L., & Künstler, A. 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Microbiological Research, 242(October 2020), 126626. https://doi.org/10.1016/j.micres.2020.126626Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA)EstudiantesInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85357/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1032491650.2023.pdf1032491650.2023.pdfTesis de Maestría en Ciencias - Biologíaapplication/pdf2483122https://repositorio.unal.edu.co/bitstream/unal/85357/2/1032491650.2023.pdf2d3d7c17b169bc139d9f319266787d8dMD52THUMBNAIL1032491650.2023.pdf.jpg1032491650.2023.pdf.jpgGenerated Thumbnailimage/jpeg4052https://repositorio.unal.edu.co/bitstream/unal/85357/3/1032491650.2023.pdf.jpg3a3fa485cc829631d890e16fa7dd240fMD53unal/85357oai:repositorio.unal.edu.co:unal/853572024-01-18 23:03:53.603Repositorio Institucional Universidad Nacional de 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