Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)

Ilustraciones

Autores:: Murillo Gómez, Paola Andrea

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
dc.title.translated.eng.fl_str_mv	Organogenesis, in vitro regeneration and expression of gus, gfp, pcht28 and PpGlu genes in common tree tomato (Solanum betaceum Cav.)
title	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
spellingShingle	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.) 630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura) Cultivos in vitro Tomate de arbol Agroinfiltración Expresión transitoria Glucanasa Gus Organogénesis Quitinasa Tomate de árbol común Agroinfiltration Transient expression Glucanase Gus Organogenesis Tree tomato
title_short	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
title_full	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
title_fullStr	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
title_full_unstemmed	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
title_sort	Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)
dc.creator.fl_str_mv	Murillo Gómez, Paola Andrea
dc.contributor.advisor.none.fl_str_mv	Hoyos Sánchez, Rodrigo Alberto Chavarriaga, Paul
dc.contributor.author.none.fl_str_mv	Murillo Gómez, Paola Andrea
dc.contributor.researchgroup.spa.fl_str_mv	Biotecnologia Vegetal
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)
topic	630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura) Cultivos in vitro Tomate de arbol Agroinfiltración Expresión transitoria Glucanasa Gus Organogénesis Quitinasa Tomate de árbol común Agroinfiltration Transient expression Glucanase Gus Organogenesis Tree tomato
dc.subject.lemb.none.fl_str_mv	Cultivos in vitro Tomate de arbol
dc.subject.proposal.none.fl_str_mv	Agroinfiltración Expresión transitoria Glucanasa Gus Organogénesis Quitinasa Tomate de árbol común
dc.subject.proposal.eng.fl_str_mv	Agroinfiltration Transient expression Glucanase Gus Organogenesis Tree tomato
description	Ilustraciones
publishDate	2021
dc.date.issued.none.fl_str_mv	2021-11
dc.date.accessioned.none.fl_str_mv	2022-10-20T21:40:43Z
dc.date.available.none.fl_str_mv	2022-10-20T21:40:43Z
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/82402
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/82402 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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A., & Tamayo, P. J. (1997). Enfermedades del cultivo del tomate de arbol en Antioquia: guia de reconocimiento y control. Corporación Colombiana de Investigación Agropecuaria, CORPOICA. https://repository.agrosavia.co/bitstream/handle/20.500.12324/1159/39500_23393.pdf?sequence=1&isAllowed=y Saldarriaga, A., Castaño, J., & Arango, R. (2008). Caracterización del agente causante de la antracnosis en tomate de árbol, manzano y mora. Revista Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 32(123), 145–156. https://doi.org/10.15713/ins.mmj.3 Sanabria, A., Mahuku, G., Kelemu, S., Cadavid, M., García, C., Hío, J. C., Martínez, É., & Osorio, J. A. (2010). Molecular identification and characterization of Colletotrichum sp. isolates from Tahiti lime, tamarillo and mango. Agronomia Colombiana, 28(3), 391–399. Tamayo M., P. J. (2001). 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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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dc.publisher.department.spa.fl_str_mv	Escuela de biociencias
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Medellín
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Hoyos Sánchez, Rodrigo Alberto77b15cd865de5695e551bcba0d6d800f600Chavarriaga, Paul56c25d00d7afea01d84e12b377f73e87Murillo Gómez, Paola Andreac7da733f2feea11171e5a9a594db6e81Biotecnologia Vegetal2022-10-20T21:40:43Z2022-10-20T21:40:43Z2021-11https://repositorio.unal.edu.co/handle/unal/82402Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/IlustracionesEl cultivo de tomate de árbol (Solanum betaceum Cav.) es un cultivo que, aunque es muy conocido y sus frutos muy consumidos en Colombia, no se encuentra tecnificado, como muchos otros cultivos en el país. Este frutal es susceptible a plagas como mosca blanca y áfidos, y a enfermedades como las causadas por virosis o por hongos, por ejemplo, la antracnosis, enfermedad causada por los hongos Colletotrichum, que causa daños principalmente en el fruto, ya sea antes o después de su cosecha, y también afecta partes jóvenes como flores y primordios foliares. Con miras a la transformación estable de tomate de árbol común para eventualmente poder generar alguna resistencia a enfermedades como la antracnosis, en este trabajo se optimizó la regeneración in vitro de esta especie vía organogénesis obteniéndose un promedio de 18 brotes por explante, en el caso de hojas. Se evaluó la expresión transitoria en hojas de tomate de árbol común in vitro, mediante el plásmido pCambia 1305.2 con el gen reportero gusPlus y la agroinfiltración de hojas en planta, esta última utilizando la cepa EHA105 de Agrobacterium tumefaciens con el plásmido pMDC85, portando los genes para una endoquitinasa (pcht28) o para una β-1,3-glucanasa (PpGlu); hasta la fecha no hay reportes de la evaluación de la expresión transitoria en hojas de tomate de árbol mediante agroinfiltración. Mediante el gen gus se encontró una eficiencia de transformación del 97,5% para la cepa EHA105, con base en el número de explantes gus positivos. Se llevaron a cabo ensayos in vitro para evaluar la expresión de los genes pcht28 y PpGlu en segmentos de hojas de tomate de árbol común, y se encontró un alto sobrecremiento de Agrobacterium y una alta tasa de muerte del tejido in vitro. (Texto tomado de la fuente)Tree tomato (Solanum betaceum Cav.) is a crop well known and its fruits widely consumed in Colombia, but it is not technified, like many other crops in the country. This fruit tree is susceptible to pests such as whiteflies and aphids, and to diseases caused by viruses or fungi, for example, anthracnose, disease caused by Colletotrichum fungi, which mainly causes damage to the fruit, either before or after harvest, and it also affects the young parts as flowers and leaf primordia. For the purpose of the stable transformation of common tree tomato, in the future, to eventually be able to generate some resistance to diseases such as anthracnose, in this work the in vitro regeneration of this species via organogenesis was optimized, obtaining an average of 18 shoots per explant, in the case of leaves. The transient expression in common tree tomato leaves in vitro was evaluated by means of the pCambia 1305.2 plasmid with the gusPlus reporter gene and the agroinfiltration of leaves in the plant, this last one using the EHA105 strain of Agrobacterium tumefaciens with the plasmid pMDC85, carrying the genes for an endochitinase (pcht28) or for a β-1,3-glucanase (PpGlu). To date there are no reports of the evaluation of transient expression in tree tomato leaves by agroinfiltration. Using the gus gene, a transformation efficiency of 97,5% was found for the EHA105 strain, based on the number of positive gus explants. In vitro assays were carried out to evaluate the expression of pcht28 and PpGlu genes in segments of common tree tomato leaves, and a high overgrowth of Agrobacterium and a high rate of tissue death in vitro were found.DoctoradoDoctor en BiotecnologíaÁrea curricular Biotecnologíaxx, 194 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Doctorado en BiotecnologíaEscuela de biocienciasFacultad de CienciasMedellínUniversidad Nacional de Colombia - Sede Medellín630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)Cultivos in vitroTomate de arbolAgroinfiltraciónExpresión transitoriaGlucanasaGusOrganogénesisQuitinasaTomate de árbol comúnAgroinfiltrationTransient expressionGlucanaseGusOrganogenesisTree tomatoOrganogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)Organogenesis, in vitro regeneration and expression of gus, gfp, pcht28 and PpGlu genes in common tree tomato (Solanum betaceum Cav.)Trabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAbdul Kadir, N. 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Acta Agronomica, 65(3), 304–311. https://doi.org/http://dx.doi.org/10.15446/acag.v65n3.49771Grupo de Biotecnología Vegetal Universidad Nacional de Colombia, sede MedellínEstudiantesInvestigadoresPúblico generalORIGINAL28719377 Paola Murillo 2021.pdf28719377 Paola Murillo 2021.pdfTesis de Doctorado en Biotecnologíaapplication/pdf5896216https://repositorio.unal.edu.co/bitstream/unal/82402/4/28719377%20Paola%20Murillo%202021.pdf465c48df942799d7c3697c16a1ca3374MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/82402/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53THUMBNAIL28719377 Paola Murillo 2021.pdf.jpg28719377 Paola Murillo 2021.pdf.jpgGenerated Thumbnailimage/jpeg5311https://repositorio.unal.edu.co/bitstream/unal/82402/5/28719377%20Paola%20Murillo%202021.pdf.jpg0897c534beeb4265dec1a52ea481fbefMD55unal/82402oai:repositorio.unal.edu.co:unal/824022023-08-09 23:04:41.877Repositorio Institucional Universidad Nacional de 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Organogénesis, regeneración in vitro y expresión de los genes gus, gfp, pcht28 y PpGlu en tomate de árbol común (Solanum betaceum Cav.)

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