Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas
ilustraciones, diagramas
- Autores:
-
Grajales Ríos, Naren Zulamir
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84846
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas::639 - Caza, pesca, conservación, tecnologías relacionadas
330 - Economía::333 - Economía de la tierra y de la energía
Conservación de suelos
Soil conservation
MICP
Sporosarcina pasteurii
Bioprecipitation
Bioremediation
Calcite
Copper
Urease
Bioprecipitación
Biorremediación
Calcita
Cobre
Ureasa
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| dc.title.translated.eng.fl_str_mv |
Biomineralization of calcium carbonate (CaCO3) induced by bacteria for the immobilization of copper ions (Cu2+) with potential for applications in agricultural soils |
| title |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| spellingShingle |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas 630 - Agricultura y tecnologías relacionadas::639 - Caza, pesca, conservación, tecnologías relacionadas 330 - Economía::333 - Economía de la tierra y de la energía Conservación de suelos Soil conservation MICP Sporosarcina pasteurii Bioprecipitation Bioremediation Calcite Copper Urease Bioprecipitación Biorremediación Calcita Cobre Ureasa |
| title_short |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| title_full |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| title_fullStr |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| title_full_unstemmed |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| title_sort |
Biomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolas |
| dc.creator.fl_str_mv |
Grajales Ríos, Naren Zulamir |
| dc.contributor.advisor.none.fl_str_mv |
Márquez Godoy, Marco Antonio |
| dc.contributor.author.none.fl_str_mv |
Grajales Ríos, Naren Zulamir |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Mineralogía Aplicada y Bioprocesos (Gmab) |
| dc.contributor.orcid.spa.fl_str_mv |
Grajales Ríos, Naren Zulamir [0009-0002-0869-7984] Márquez Godoy, Marco Antonio [0000-0002-7462-2430] |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas::639 - Caza, pesca, conservación, tecnologías relacionadas 330 - Economía::333 - Economía de la tierra y de la energía |
| topic |
630 - Agricultura y tecnologías relacionadas::639 - Caza, pesca, conservación, tecnologías relacionadas 330 - Economía::333 - Economía de la tierra y de la energía Conservación de suelos Soil conservation MICP Sporosarcina pasteurii Bioprecipitation Bioremediation Calcite Copper Urease Bioprecipitación Biorremediación Calcita Cobre Ureasa |
| dc.subject.lemb.spa.fl_str_mv |
Conservación de suelos |
| dc.subject.lemb.eng.fl_str_mv |
Soil conservation |
| dc.subject.proposal.eng.fl_str_mv |
MICP Sporosarcina pasteurii Bioprecipitation Bioremediation Calcite Copper Urease |
| dc.subject.proposal.spa.fl_str_mv |
Bioprecipitación Biorremediación Calcita Cobre Ureasa |
| description |
ilustraciones, diagramas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-10-27T20:37:41Z |
| dc.date.available.none.fl_str_mv |
2023-10-27T20:37:41Z |
| dc.date.issued.none.fl_str_mv |
2023-02-01 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84846 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84846 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
| language |
spa |
| dc.relation.indexed.spa.fl_str_mv |
RedCol LaReferencia |
| dc.relation.references.spa.fl_str_mv |
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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Márquez Godoy, Marco Antonio50146bcaff8cef6053ef23fabfa599b2Grajales Ríos, Naren Zulamire14e73851578518bf1b717a48b474702Grupo de Mineralogía Aplicada y Bioprocesos (Gmab)Grajales Ríos, Naren Zulamir [0009-0002-0869-7984]Márquez Godoy, Marco Antonio [0000-0002-7462-2430]2023-10-27T20:37:41Z2023-10-27T20:37:41Z2023-02-01https://repositorio.unal.edu.co/handle/unal/84846Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasDebido a las diversas actividades industriales y humanas, los metales pesados se han convertido en un contaminante que se ha extendido por todo el medio ambiente, y sus concentraciones superiores a los límites ambientales es un problema grave. La precipitación de carbonatos inducida microbiológicamente (MICP) se ha explorado para la inmovilización de metales pesados en diferentes matrices medioambientales en donde los microorganismos tienen la capacidad de sintetizar biominerales, esto ofrece una manera eficiente de inmovilizar e incorporar metales pesados como el cobre, dentro de fases sólidas estables y cristalinas. Lo expuesto justifica la necesidad de buscar otras alternativas como la MICP como potencial proceso de biorremediación. Para ello, se estudió el efecto de la biomineralización de carbonato inducido por bacterias y la incorporación de cobre (Cu2+) para su inmovilización como base para su aplicación en suelos agrícolas. Se evaluó la tolerancia a cuatro concentraciones de cobre (100, 250, 500 y 1000 mg/l) de 13 bacterias de la Colección Alemana de Microorganismos y Cultivos Celulares reportadas en la literatura con capacidad calcificante. Posterior a la selección de las bacterias tolerantes a dos concentraciones de cobre y con la mayor capacidad de precipitar carbonatos, se seleccionaron tres bacterias para la bioprecipitación e inmovilización de cobre, evaluando seis variables, tanto químicas como biológicas. Los precipitados obtenidos se caracterizaron por medio de las técnicas de Espectroscopía Infrarroja con Transformada de Fourier (FTIR), Difracción de Rayos X (DRX) y Microscopía Electrónica de Barrido acoplada a Espectroscopia de Rayos X (SEM/EDX). Los resultados mostraron que 10 de los 13 microorganismos evaluados son capaces de tolerar las concentraciones de cobre a 100 y 250 mg/l, cabe destacar, que el género Sporosarcina es uno de los más tolerantes y con la mayor producción de carbonatos seguido de Corynebacterium ammoniagenes, S. ureae y Sutcliffiella cohnii. En los tratamientos en suspensión se pudo determinar que S. pasteurii DSM-276, es el microorganismo que tiene mejor eficiencia de eliminación del cobre a pH mayor de 7.5 con valores superiores al 60% y 40% a 100 y 250 mg/l respectivamente. El análisis por DRX de los precipitados mostraron la presencia de calcita y vaterita como los principales fases minerales formadas en todos los tratamientos, acompañados con una pequeña cantidad de weddellita y malaquita, en los tratamientos en presencia de cobre, asimismo, la morfología de los cristales y su porcentaje de composición elemental varió según la presencia o ausencia de este metal pesado. Estos resultados indican que el cobre en el proceso de la MICP puede coprecipitar junto con el carbonato de calcio e incluso, ser inmovilizado en la estructura cristalina a través de reacciones mediadas biológicamente que pueden afectar la mineralogía y morfología de los minerales precipitados. (Texto tomado de la fuente)Due to various industrial and human activities, heavy metals have become a contaminant that has become widespread throughout the environment, and their concentrations above environmental limits is a fundamental problem. Microbiologically induced carbonate precipitation (MICP) has been explored for the immobilization of heavy metals in different environmental matrices where microorganisms have the ability to synthesize biominerals, this offers an efficient way to immobilize and incorporate heavy metals such as copper, within stable and crystalline solid phases. The above justifies the need to look for other alternatives such as MICP as a potential bioremediation process. For this purpose, the effect of bacteria-induced carbonate biomineralization and the incorporation of copper (Cu2+) for its immobilization was studied as a basis for its application in agricultural soils. The tolerance to four copper concentrations (100, 250, 500 and 1000 mg/l) of 13 bacteria from the German Collection of Microorganisms and Cell Cultures reported in the literature with calcifying capacity was evaluated. After the selection of bacteria tolerant to two copper concentrations and with the highest capacity to precipitate carbonates, three bacteria were selected for bioprecipitation and immobilization of copper, evaluating six variables, both chemical and biological. The precipitates obtained were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and Scanning Electron Microscopy coupled to X-Ray Spectroscopy (SEM/EDX). The results showed that 10 of the 13 microorganisms evaluated are able to tolerate copper concentrations at 100 and 250 mg/l, however, the genus Sporosarcina is one of the most tolerant and with the highest production of carbonates followed by Corynebacterium ammoniagenes, S. ureae and Sutcliffiella cohnii. In the suspension treatments, it was determined that S. pasteurii DSM-276 is the microorganism with the best copper removal efficiency at pH greater than 7.5, with values higher than 60% and 40% at 100 and 250 mg/l, respectively. XRD analysis of the precipitates showed the presence of calcite and vaterite as the main mineral phases formed in all treatments, accompanied by a small amount of weddellite and malachite, in the treatments in the presence of copper, also, the morphology of the crystals and their percentage of elemental composition varied according to the presence or absence of this heavy metal. These results indicate that copper in the MICP process can co-precipitate with calcium carbonate and even be immobilized in the crystal structure through biologically mediated reactions that can affect the mineralogy and morphology of the precipitated minerals.MaestríaMagíster en Ciencias - BiotecnologíaBiotecnología AmbientalBiotecnología MicrobianaÁrea Curricular Biotecnologíaxviii, 97 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín630 - Agricultura y tecnologías relacionadas::639 - Caza, pesca, conservación, tecnologías relacionadas330 - Economía::333 - Economía de la tierra y de la energíaConservación de suelosSoil conservationMICPSporosarcina pasteuriiBioprecipitationBioremediationCalciteCopperUreaseBioprecipitaciónBiorremediaciónCalcitaCobreUreasaBiomineralización de carbonato de calcio (CaCO3) inducido por bacterias para la inmovilización de iones cobre (Cu2+) con potencial para aplicaciones en suelos agrícolasBiomineralization of calcium carbonate (CaCO3) induced by bacteria for the immobilization of copper ions (Cu2+) with potential for applications in agricultural soilsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferenciaAchal, V., Pan, X., Fu, Q., & Zhang, D. 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Frontiers in Bioengineering and Biotechnology, 4(JAN), 1–21. https://doi.org/10.3389/fbioe.2016.00004BibliotecariosEstudiantesGrupos comunitariosInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84846/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1017235688.2023..pdf1017235688.2023..pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf4420720https://repositorio.unal.edu.co/bitstream/unal/84846/2/1017235688.2023..pdf39bfef221ab1dd700c99686dcdfbc8f2MD52THUMBNAIL1017235688.2023..pdf.jpg1017235688.2023..pdf.jpgGenerated Thumbnailimage/jpeg5686https://repositorio.unal.edu.co/bitstream/unal/84846/3/1017235688.2023..pdf.jpg884ba1780a18b0487d1c96d065d00b8dMD53unal/84846oai:repositorio.unal.edu.co:unal/848462024-08-19 23:10:35.315Repositorio Institucional Universidad Nacional de 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