Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno

ilustraciones, diagramas, fotografías

Autores:: Vargas Moreno, Monica Alexandra

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/84339
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
dc.title.translated.eng.fl_str_mv	Potential neuroprotective effect of LXR agonists on autophagy activation on glial and neuronal cells subjected to glucose and oxygen deprivation
title	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
spellingShingle	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno 610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades Autofagia Glioma Autophagy Autofagia LXR Isquemia cerebral LC3-II Neuroprotección Autophagy Brain ischemia Neuroprotection
title_short	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
title_full	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
title_fullStr	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
title_full_unstemmed	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
title_sort	Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno
dc.creator.fl_str_mv	Vargas Moreno, Monica Alexandra
dc.contributor.advisor.none.fl_str_mv	Arboleda Bustos, Gonzalo Humberto
dc.contributor.author.none.fl_str_mv	Vargas Moreno, Monica Alexandra
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Neurociencias-Universidad Nacional de Colombia
dc.subject.ddc.spa.fl_str_mv	610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades
topic	610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades Autofagia Glioma Autophagy Autofagia LXR Isquemia cerebral LC3-II Neuroprotección Autophagy Brain ischemia Neuroprotection
dc.subject.decs.spa.fl_str_mv	Autofagia Glioma
dc.subject.decs.eng.fl_str_mv	Autophagy
dc.subject.proposal.spa.fl_str_mv	Autofagia LXR Isquemia cerebral LC3-II Neuroprotección
dc.subject.proposal.eng.fl_str_mv	Autophagy Brain ischemia Neuroprotection
description	ilustraciones, diagramas, fotografías
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-07-27T21:32:25Z
dc.date.available.none.fl_str_mv	2023-07-27T21:32:25Z
dc.date.issued.none.fl_str_mv	2023-07-26
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/84339
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/84339 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.publisher.faculty.spa.fl_str_mv	Facultad de Medicina
dc.publisher.place.spa.fl_str_mv	Bogotá,Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Arboleda Bustos, Gonzalo Humberto8d01100986b4e816de54bb4d3f52f1f0Vargas Moreno, Monica Alexandrac0d82150cab7e6e81344b2c6bb15eadaGrupo de Neurociencias-Universidad Nacional de Colombia2023-07-27T21:32:25Z2023-07-27T21:32:25Z2023-07-26https://repositorio.unal.edu.co/handle/unal/84339Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasLa enfermedad cerebrovascular es una de las causas más relevantes de morbimortalidad en el mundo. Por ello es de gran importancia el entendimiento de los mecanismos fisiopatológicos de esta enfermedad, que permitan encontrar blancos terapéuticos para mejorar la sobrevida neuronal que prosigue a la lesión isquémica. Clásicamente se hablaba de 2 tipos de muerte celular relacionadas con este tipo de lesión: necrosis y apoptosis; sin embargo, recientemente se ha encontrado una gran variedad morfológica de tipos adicionales de muerte celular, entre estas, la asociada a autofagia ha tomado gran importancia en la última década. Existe evidencia reciente del efecto neuroprotector de los agonistas de receptores X del hígado (LXR: por sus siglas en inglés, Liver X receptors) en escenarios de isquemia cerebral y de su capacidad de modular la activación de la autofagia. Se ha descrito también el papel que cumplen las células gliales en el daño neuronal que prosigue a la lesión isquémica. Debido a esto, se planteó como objetivo general analizar el efecto de agonistas LXR en la activación de procesos de autofagia en células gliales y neuronales expuestas a privación de glucosa y oxígeno como un posible blanco terapéutico para modular la respuesta celular deletérea post isquemia. Se desarrolló un modelo in vitro de isquemia celular con líneas celulares y cultivo primario de neuronas y células gliales de corteza de ratón expuestas a privación de glucosa y oxígeno pretratadas con un extracto de plantas colombianas con actividad agonista LXR, Zanthoxylum caribaeum. Encontrando que el pretratamiento con las fracciones en estudio ofrece protección ante la privación combinada de glucosa y oxígeno en líneas celulares y células gliales de cultivo primario y en estas últimas podría activar vías de autofagia evidenciado en el aumentó de los niveles de LC3II. (Texto tomado de la fuente)Cerebrovascular disease is one of the most relevant causes of morbimortality in the world. Therefore, the understanding of the physio pathological disease mechanisms that will allow the acquisition of therapeutic targets to improve neuronal survival after an ischemic lesion is of the utmost importance. Traditionally, two types of cell death were associated with this kind of lesion: necrosis and apoptosis, however as of late a great variety of morphological types of cellular death has been found, among those, the one associated with autophagy has gained great importance in the last decade. There is recent evidence of the neuroprotective effect of the Liver X receptor (LXR) agonists in scenarios of cerebral ischemia and their ability to modulate autophagy activation. The role of glial cells in neuronal damage after an ischemic injury has also been described. Due to this, the analyzing the effect of the LXR agonists on the activation of the autophagy processes on glial and neuronal cells exposed to glucose and oxygen deprivation as a possible therapeutic target to modulate the deleterious post ischemic cell response was proposed as a primary objective. An in vitro model of cellular ischemia was developed, using cell lines, neuron primary cultures, as well as glial cortex cells obtained from mice, all of which were exposed to glucose and oxygen deprivation and pretreated with and extract obtained from Colombian plants with an LXR agonist activity, Zanthoxylum caribaeum. We found that pretreatment with the studied fractions confers protection against the combined deprivation of glucose and oxygen in cell lines and in primary culture glial cells, and in the latter, might activate autophagy pathways, as demonstrated by an increase in LC3II levels.MaestríaMagíster en NeurocienciasMuerte celular94 páginasapplication/pdfspa610 - Medicina y salud::615 - Farmacología y terapéutica610 - Medicina y salud::616 - EnfermedadesAutofagiaGliomaAutophagyAutofagiaLXRIsquemia cerebralLC3-IINeuroprotecciónAutophagyBrain ischemiaNeuroprotectionPotencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígenoPotential neuroprotective effect of LXR agonists on autophagy activation on glial and neuronal cells subjected to glucose and oxygen deprivationTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBogotá - Medicina - Maestría en NeurocienciasFacultad de MedicinaBogotá,ColombiaUniversidad Nacional de Colombia - Sede BogotáAgrotis, A., Pengo, N., Burden, J. 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The Yale journal of biology and medicine, 80(2), 51–60.“Búsqueda racional de alcaloides isoquinolínicos del género Zanthoxylum (Rutaceae) como posibles agentes neuroprotectores para el tratamiento de la enfermedad de Alzheimer"Financiado por MINCIENCIAS Código 110177758004, convocatoria 777-2017; RC-854 de 2017EstudiantesInvestigadoresMaestrosPúblico generalORIGINAL1023892304.2023.pdf1023892304.2023.pdfTesis de Maestría en Neurocienciasapplication/pdf1581170https://repositorio.unal.edu.co/bitstream/unal/84339/2/1023892304.2023.pdfb86250f5cbedffdd1ea7aa674f2d15faMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84339/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL1023892304.2023.pdf.jpg1023892304.2023.pdf.jpgGenerated Thumbnailimage/jpeg5233https://repositorio.unal.edu.co/bitstream/unal/84339/3/1023892304.2023.pdf.jpg2dcd2013aef73c14e016fdf0a30ff3d4MD53unal/84339oai:repositorio.unal.edu.co:unal/843392023-08-14 23:04:52.982Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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Potencial efecto neuroprotector de los agonistas LXR en la activación de autofagia de células gliales y neuronales sometidas a privación de glucosa y oxígeno

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