Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano

ilustraciones, diagramas, fotografías a color

Autores:: Ramírez Ambrosio, Oscar Mauricio

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
dc.title.translated.eng.fl_str_mv	Relevance of the Insulin-like growth factor receptor (IGF-1R) in the human trophoblast pluripotent cell phenotype
title	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
spellingShingle	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano 570 - Biología::572 - Bioquímica Adult Germline Stem Cells Stem Cells Embryonic Stem Cells Células madre Células Madre Embrionarias Células Madre Germinales Adultas Pluripotencia Cáncer Factores de crecimiento Trofoblasto Pluripotency Cancer Growth Factors Trophoblast
title_short	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
title_full	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
title_fullStr	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
title_full_unstemmed	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
title_sort	Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano
dc.creator.fl_str_mv	Ramírez Ambrosio, Oscar Mauricio
dc.contributor.advisor.none.fl_str_mv	Umaña Pérez, Adriana
dc.contributor.author.none.fl_str_mv	Ramírez Ambrosio, Oscar Mauricio
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Hormonas
dc.subject.ddc.spa.fl_str_mv	570 - Biología::572 - Bioquímica
topic	570 - Biología::572 - Bioquímica Adult Germline Stem Cells Stem Cells Embryonic Stem Cells Células madre Células Madre Embrionarias Células Madre Germinales Adultas Pluripotencia Cáncer Factores de crecimiento Trofoblasto Pluripotency Cancer Growth Factors Trophoblast
dc.subject.decs.eng.fl_str_mv	Adult Germline Stem Cells Stem Cells Embryonic Stem Cells
dc.subject.decs.spa.fl_str_mv	Células madre Células Madre Embrionarias
dc.subject.lemb.spa.fl_str_mv	Células Madre Germinales Adultas
dc.subject.proposal.spa.fl_str_mv	Pluripotencia Cáncer Factores de crecimiento Trofoblasto
dc.subject.proposal.eng.fl_str_mv	Pluripotency Cancer Growth Factors Trophoblast
description	ilustraciones, diagramas, fotografías a color
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-08-14T16:06:31Z
dc.date.available.none.fl_str_mv	2023-08-14T16:06:31Z
dc.date.issued.none.fl_str_mv	2023
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Other
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/84551
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/84551 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.format.extent.spa.fl_str_mv	xvi, 73 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Umaña Pérez, Adrianae39bd65085b0606e03be9eca9e2102e2Ramírez Ambrosio, Oscar Mauricio93393053e312a3c3030ec3117d9f4fa2Grupo de Investigación en Hormonas2023-08-14T16:06:31Z2023-08-14T16:06:31Z2023https://repositorio.unal.edu.co/handle/unal/84551Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías a colorDiversos estudios han puesto de manifiesto la relación entre la señalización celular mediada por el receptor del Factor de Crecimiento similar a Insulina 1 (IGF-1R), la expresión de factores de pluripotencia canónicos, el cáncer y la enfermedad trofoblástica gestacional (ETG), sin embargo, se desconoce la relevancia de la señalización del IGF-1R mediada por IGF2, principal factor de crecimiento expresado durante la gestación, en la expresión de los factores de pluripotencia Oct4, Sox2 y Nanog en trofoblasto. Para acercarnos a este interrogante, se utilizó como modelo biológico la línea celular proveniente de trofoblasto humano del primer trimestre de gestación HTR-8/SVneo y una línea derivada de ella por silenciamiento estable para el IGF-1R (HTR-8/SVneo shIGF-IR). Ambas líneas celulares crecieron bajo privación de suero fetal bovino en presencia o ausencia de IGF2 10 nM durante 12, 24, 36, 48 y 72h. Los genes de interés se cuantificaron mediante RT-qPCR. HTR-8/SVneo presenta una sobreexpresión Masiva Aguda de los tres Factores de Pluripotencia (SEMA-FP) a las 12h respecto a la condición basal (Nanog: 43 veces, Oct4: 10 veces, Sox2: 8 veces, p<0.001) dependiente de niveles basales de IGF-1R, como respuesta a la privación de suero, probablemente como mecanismo de protección frente al estrés celular, manteniendo su potencial de diferenciación y de regeneración de sus subpoblaciones celulares. Oct4, Sox2 y Nanog presentan expresión mínima (basal) de transcrito independiente de la presencia de suero e IGF2, no obstante, el suero inhibe la SEMA-FP de los tres factores, mientras que IGF2, en ausencia de suero, inhibe parcialmente la de Oct4 y Nanog, pero no la de Sox2. Estas inhibiciones pueden depender o no de la existencia de niveles basales de IGF-1R para cada uno de los tres factores. La detección de los tres FP es retadora a nivel de proteína en HTR-8/SVneo debido a su baja expresión, la cual se limita a subpoblaciones celulares específicas. IGF-1R y Nanog se postulan como blancos moleculares a ser tenidos en cuenta en estudios dirigidos al tratamiento de ETG como coriocarcinoma, particularmente aquellos que poseen células madre del cáncer y presentan resistencia a tratamientos convencionales. (Texto tomado de la fuente)Several studies have revealed the link between the Insulin-like Growth Factor 1 receptor (IGF-1R) mediated cell signaling, the expression of canonical pluripotency factors, cancer and gestational trophoblastic disease (GTD). Nevertheless, the relevance of IGF-1R mediated IGF2 (the main growth factor expressed during pregnancy) regulation of Oct4, Sox2 and Nanog pluripotency factors in trophoblasts is unknown. To approach this question, we used as biological models the first trimester derived human trophoblast cell line HTR-8/SVneo and a HTR-8/SVneo derived IGF-1R stable silenced cell line. Both cell lines grew under fetal bovine serum deprivation in the presence or absence of IGF2 10 nM for 12, 24, 36, 48 and 72h. The genes of interest were quantified by RT-qPCR. HTR-8/SVneo showed an Acute Massive Overexpression of the three Pluripotency Factors (AMO-PF) at 12h compared to the baseline condition (Nanog: 43-fold, Oct4: 10-fold and Sox2: 8-fold, p<0.001-0.0001) dependent on basal IGF-1R levels, as a response to serum deprivation, probably as a protection mechanism against cellular stress, in order to maintain the cell subpopulation regenerative and differentiation potential. Oct4, Sox2 and Nanog showed a transcript expression minimum (baseline), independent of serum and IGF2 presence, nevertheless, serum inhibits the AMO-PF of all three factors, while IGF2, in the absence of serum, partially inhibits Oct4 and Nanog but not Sox2. The dependence of these inhibitions on IGF-1R baseline levels is specific for each of the three factors. Detection of all three PFs is particularly challenging at the protein level in HTR-8/SVneo due to their low expression, which is limited to specific cell subpopulations. We propose IGF-1R and Nanog as molecular targets to be considered in GTD (such as choriocarcinoma) treatment studies, particularly those including cancer stem cells and showing conventional treatment resistance.MaestríaMagíster en Ciencias - BioquímicaFactores de crecimiento, diferenciación y cáncerxvi, 73 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaAdult Germline Stem CellsStem CellsEmbryonic Stem CellsCélulas madreCélulas Madre EmbrionariasCélulas Madre Germinales AdultasPluripotenciaCáncerFactores de crecimientoTrofoblastoPluripotencyCancerGrowth FactorsTrophoblastRelevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humanoRelevance of the Insulin-like growth factor receptor (IGF-1R) in the human trophoblast pluripotent cell phenotypeTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionOtherhttp://purl.org/redcol/resource_type/TMAbou-Kheir, W., Barrak, J., Hadadeh, O., & Daoud, G. 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In Signal Transduction and Targeted Therapy (Vol. 6, Issue 1). https://doi.org/10.1038/s41392-020-00430-1MincienciasDivisión de Investigación Sede Bogotá - DIBInvestigadoresORIGINAL1020757401.2023.pdf1020757401.2023.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf1938439https://repositorio.unal.edu.co/bitstream/unal/84551/4/1020757401.2023.pdfc898d1ad58ccf4dbe3e99559a72be7ecMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84551/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53THUMBNAIL1020757401.2023.pdf.jpg1020757401.2023.pdf.jpgGenerated Thumbnailimage/jpeg5377https://repositorio.unal.edu.co/bitstream/unal/84551/5/1020757401.2023.pdf.jpg1ede04a2e8229fa1f89251570eabfe94MD55unal/84551oai:repositorio.unal.edu.co:unal/845512024-07-28 01:12:09.479Repositorio Institucional Universidad Nacional de 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Relevancia del receptor del factor de crecimiento similar a insulina (IGF-1R) en el fenotipo celular pluripotente de trofoblasto humano

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