Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp

ilustraciones, imágenes, tablas

Autores:: Beltrán, Óscar Gabriel

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
dc.title.translated.eng.fl_str_mv	Identification and classification of the metabolic pathways of Leptospira spp species
title	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
spellingShingle	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp 570 - Biología::579 - Historia natural microorganismos, hongos, algas Metabolitos microbiano Microbial metabolites Análisis basado restricciones Clado Cultivo Genoma Leptospira Metabolismo Modelos metabólicos a escala genómica Clade Culture Constraint-based analyses Genome Genome-scale metabolic models Leptospira Metabolism
title_short	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
title_full	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
title_fullStr	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
title_full_unstemmed	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
title_sort	Identificación y clasificación de las rutas metabólicas de las especies de Leptospira spp
dc.creator.fl_str_mv	Beltrán, Óscar Gabriel
dc.contributor.advisor.none.fl_str_mv	Pinzón Velasco, Andrés Mauricion
dc.contributor.author.none.fl_str_mv	Beltrán, Óscar Gabriel
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Bioinformática y Biologı́a de Sistemas
dc.subject.ddc.spa.fl_str_mv	570 - Biología::579 - Historia natural microorganismos, hongos, algas
topic	570 - Biología::579 - Historia natural microorganismos, hongos, algas Metabolitos microbiano Microbial metabolites Análisis basado restricciones Clado Cultivo Genoma Leptospira Metabolismo Modelos metabólicos a escala genómica Clade Culture Constraint-based analyses Genome Genome-scale metabolic models Leptospira Metabolism
dc.subject.lemb.none.fl_str_mv	Metabolitos microbiano Microbial metabolites
dc.subject.proposal.spa.fl_str_mv	Análisis basado restricciones Clado Cultivo Genoma Leptospira Metabolismo Modelos metabólicos a escala genómica
dc.subject.proposal.eng.fl_str_mv	Clade Culture Constraint-based analyses Genome Genome-scale metabolic models Leptospira Metabolism
description	ilustraciones, imágenes, tablas
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-10-15T14:19:04Z
dc.date.available.none.fl_str_mv	2021-10-15T14:19:04Z
dc.date.issued.none.fl_str_mv	2021
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/80560
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/80560 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pinzón Velasco, Andrés Mauricion5faf04f97be727ec4c5b609a4c2cc6d7Beltrán, Óscar Gabrielab3676e596db6d765681a67dccca964aGrupo de Investigación en Bioinformática y Biologı́a de Sistemas2021-10-15T14:19:04Z2021-10-15T14:19:04Z2021https://repositorio.unal.edu.co/handle/unal/80560Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, imágenes, tablasEl aislamiento de Leptospira spp. es considerado como la prueba de laboratorio de mayor fiabilidad para el diagnostico de leptospirosis. Sin embargo, las limitaciones del cultivo impiden su implementación para el diagnostico de rutina y han retrasado el desarrollo de nuevas alternativas diagnósticas y terapéuticas. El objetivo de este trabajo fue identificar y caracterizar de las rutas metabólicas del género Leptospira a partir de su contenido genómico y con ello explorar las capacidades metabólicas de este microorganismo. De acuerdo con lo anterior, se encontraron cinco grupos monofiléticos que concuerdan con su estado de patogenicidad pero que difieren en parte de su contenido genómico. Del mismo modo, se realizaron reconstrucciones de modelos metabólicos a escala genómica que demostraron la presencia de distintas rutas metabólicas en los clados, así como respuestas diferenciales durante análisis basados en restricciones. Lo anterior sugiere que las diferencias en los contenidos genómicos provienen de procesos adaptativos y se asocian a los diversos fenotipos observados durante el cultivo. Estos resultados proponen que los miembros de los distintos clados tienen diferentes requisitos nutricionales y que estos metabolitos tienen el potencial de ser utilizados para la diferenciación de especies, como blancos farmacológicos y/o suplementos para la formulación de nuevos medios de cultivo o para ajustar los existentes. (Texto tomado de la fuente)The Leptospira isolation is considered the most reliable laboratory test for leptospirosis diagnosis. However, the culture limitations has hampered its implementation for routine diagnosis and has delayed the development of new diagnostic and therapeutic alternatives. The objective of this work was to identify and characterize the metabolic pathways of the genus Leptospira based on its genomic content and thereby to explore the metabolic capacities of this microorganism. In accordance with the above, five monophyletic groups were found that agree with their pathogenicity status but differ in part in their genomic content. In the same way, genome-scale metabolic models demonstrated the presence of different metabolic pathways in the clades, as well as differential responses during constraint-based analyses. This suggests that the genomic content differences stem from adaptive processes and they are associated with the several phenotypes observed during cultivation. These results propose that the different clades members have variations in nutritional requirements and that these metabolites have the potential to be used for the species differentiation, as drug targets and/or supplements for the formulation of new culture media or to adjust existing ones.MaestríaMagíster en BioinformáticaIn silicoModelado computacionalxii, 70 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ingeniería - Maestría en BioinformáticaDepartamento de Ingeniería de Sistemas e IndustrialFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::579 - Historia natural microorganismos, hongos, algasMetabolitos microbianoMicrobial metabolitesAnálisis basado restriccionesCladoCultivoGenomaLeptospiraMetabolismoModelos metabólicos a escala genómicaCladeCultureConstraint-based analysesGenomeGenome-scale metabolic modelsLeptospiraMetabolismIdentificación y clasificación de las rutas metabólicas de las especies de Leptospira sppIdentification and classification of the metabolic pathways of Leptospira spp speciesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMVincent A, Schiettekatte O, Goarant C, Neela V, Bernet E, Thibeaux R, et al. 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