Bovine leukosis virus detection in creole colombian breeds using nested-pcr

Using 360 DNA samples from eight Creole bovine breeds Blanco Orejinegro (BON), Casanareño (CAS), Costeño con Cuernos (CCC), Chino Santandereano (ChS), Caqueteño (CQT), Hartón del Valle (HV), Romosinuano (RS) and San Martinero (SM), two synthetic Colombian breeds: Lucerna (LUC) and Velásquez (VEL) an...

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Autores:
Hernández Herrera, Darwin Yovanny
Posso Terranova, Andres Mauricio
Antonio Benavides, Javier
Muñoz-Flórez, Jaime Eduardo
Giovambattista, Guillermo
Tipo de recurso:
Article of journal
Fecha de publicación:
2011
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/39993
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/39993
http://bdigital.unal.edu.co/30090/
Palabra clave:
6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Diagnóstico molecular
ganado criollo
leucosis bovina enzoótica.
Creole cattle
enzootic bovine leukosis
molecular diagnostic
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_b4252c59d73019fab01662970d0217f3
oai_identifier_str oai:repositorio.unal.edu.co:unal/39993
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Bovine leukosis virus detection in creole colombian breeds using nested-pcr
title Bovine leukosis virus detection in creole colombian breeds using nested-pcr
spellingShingle Bovine leukosis virus detection in creole colombian breeds using nested-pcr
6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Diagnóstico molecular
ganado criollo
leucosis bovina enzoótica.
Creole cattle
enzootic bovine leukosis
molecular diagnostic
title_short Bovine leukosis virus detection in creole colombian breeds using nested-pcr
title_full Bovine leukosis virus detection in creole colombian breeds using nested-pcr
title_fullStr Bovine leukosis virus detection in creole colombian breeds using nested-pcr
title_full_unstemmed Bovine leukosis virus detection in creole colombian breeds using nested-pcr
title_sort Bovine leukosis virus detection in creole colombian breeds using nested-pcr
dc.creator.fl_str_mv Hernández Herrera, Darwin Yovanny
Posso Terranova, Andres Mauricio
Antonio Benavides, Javier
Muñoz-Flórez, Jaime Eduardo
Giovambattista, Guillermo
dc.contributor.author.spa.fl_str_mv Hernández Herrera, Darwin Yovanny
Posso Terranova, Andres Mauricio
Antonio Benavides, Javier
Muñoz-Flórez, Jaime Eduardo
Giovambattista, Guillermo
dc.subject.ddc.spa.fl_str_mv 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
topic 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Diagnóstico molecular
ganado criollo
leucosis bovina enzoótica.
Creole cattle
enzootic bovine leukosis
molecular diagnostic
dc.subject.proposal.spa.fl_str_mv Diagnóstico molecular
ganado criollo
leucosis bovina enzoótica.
Creole cattle
enzootic bovine leukosis
molecular diagnostic
description Using 360 DNA samples from eight Creole bovine breeds Blanco Orejinegro (BON), Casanareño (CAS), Costeño con Cuernos (CCC), Chino Santandereano (ChS), Caqueteño (CQT), Hartón del Valle (HV), Romosinuano (RS) and San Martinero (SM), two synthetic Colombian breeds: Lucerna (LUC) and Velásquez (VEL) and two introduced breeds Brahmán (B) and Holstein (H); the presence of Bovine Leukosis Virus (BLV) was evaluated through the amplification of a viral gene region env (provirus detection - nested-PCR). The percentage of presence and independence test were calculated (X²). Presence of BLV was higher in HV breed, followed by ChS (83.3% and 60% respectively); VEL and LUC breeds showed the same percentage (50%). In CAS, CCC and CQT the presence of virus was 26.7%, 23.3% y 16.7% respectively. On the other hand, no virus presence was found in BON, SM and RS. For the introduced breeds the presence of virus was 83.3% for H and 6.7% for B. The average of presence for Creole bovine breeds was lower than introduced breeds. A high and significant dependence was found between the presence of BLV with breed, sex and sampling places. The presence was lower in males than in females and in the northern than the southwestern and central areas of the country.
publishDate 2011
dc.date.issued.spa.fl_str_mv 2011-10-01
dc.date.accessioned.spa.fl_str_mv 2019-06-28T09:27:43Z
dc.date.available.spa.fl_str_mv 2019-06-28T09:27:43Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.content.spa.fl_str_mv Text
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dc.identifier.issn.spa.fl_str_mv ISSN: 2323-0118
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/39993
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/30090/
identifier_str_mv ISSN: 2323-0118
url https://repositorio.unal.edu.co/handle/unal/39993
http://bdigital.unal.edu.co/30090/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/acta_agronomica/article/view/28845
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
dc.relation.references.spa.fl_str_mv Hernández Herrera, Darwin Yovanny and Posso Terranova, Andres Mauricio and Antonio Benavides, Javier and Muñoz Flórez, Jaime Eduardo and Giovambattista, Guillermo (2011) Bovine leukosis virus detection in creole colombian breeds using nested-pcr. Acta Agronómica, 60 (4). pp. 312-318. ISSN 2323-0118
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia Sede Palmira
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/39993/1/28845-103440-2-PB.pdf
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Hernández Herrera, Darwin Yovannybfc995a5-c639-43c0-96a2-3cf120e70a4c300Posso Terranova, Andres Mauricio3b902169-3647-4a51-a027-269bae318722300Antonio Benavides, Javierb2f47cb1-ee1b-48d1-8e02-4dcb9fc4344a300Muñoz-Flórez, Jaime Eduardo52c8024e-32ea-4b48-a6e5-b01196adc425300Giovambattista, Guillermod8b8bf55-0511-49a1-b9ea-63f4dfa2cce83002019-06-28T09:27:43Z2019-06-28T09:27:43Z2011-10-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/39993http://bdigital.unal.edu.co/30090/Using 360 DNA samples from eight Creole bovine breeds Blanco Orejinegro (BON), Casanareño (CAS), Costeño con Cuernos (CCC), Chino Santandereano (ChS), Caqueteño (CQT), Hartón del Valle (HV), Romosinuano (RS) and San Martinero (SM), two synthetic Colombian breeds: Lucerna (LUC) and Velásquez (VEL) and two introduced breeds Brahmán (B) and Holstein (H); the presence of Bovine Leukosis Virus (BLV) was evaluated through the amplification of a viral gene region env (provirus detection - nested-PCR). The percentage of presence and independence test were calculated (X²). Presence of BLV was higher in HV breed, followed by ChS (83.3% and 60% respectively); VEL and LUC breeds showed the same percentage (50%). In CAS, CCC and CQT the presence of virus was 26.7%, 23.3% y 16.7% respectively. On the other hand, no virus presence was found in BON, SM and RS. For the introduced breeds the presence of virus was 83.3% for H and 6.7% for B. The average of presence for Creole bovine breeds was lower than introduced breeds. A high and significant dependence was found between the presence of BLV with breed, sex and sampling places. The presence was lower in males than in females and in the northern than the southwestern and central areas of the country.Se evaluó la presencia del virus de la leucosis bovina (VLB) en 360 muestras de ADN de ocho razas bovinas criollas Blanco Orejinegro (BON), Casanareño (CAS), Costeño con Cuernos (CCC), Chino Santandereano (ChS), Caqueteño (CQT), Hartón del Valle (HV), Romosinuano (RS) y San Martinero (SM), dos Razas Sintéticas Colombianas: Lucerna (LUC) y Velásquez (VEL) y dos razas foráneas: Brahmán (B) y Holstein (H). Para la detección del pro-virus se amplificó una región del gen env viral, mediante PCR anidada. La presencia del VLB fue mayor en la raza HV seguido por ChS (83.3% y 60% respectivamente), VEL y LUC tuvieron el mismo porcentaje (50%), en CAS, CCC y CQT la presencia del virus fue de 26.7%, 23.3% y 16.7% respectivamente; no se encontró el virus en BON, SM y RS. En las razas foráneas la presencia fue de 83.3% para H y 6.7% para B. Se encontró dependencia altamente significativa entre la presencia del VLB y la raza, el sexo y región de origen de la muestra. El promedio de presencia en las razas criollas fue menor que en las foráneas, menor en los machos que en las hembras y en la región norte que en el suroccidente y el centro del país.application/pdfspaUniversidad Nacional de Colombia Sede Palmirahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/28845Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaHernández Herrera, Darwin Yovanny and Posso Terranova, Andres Mauricio and Antonio Benavides, Javier and Muñoz Flórez, Jaime Eduardo and Giovambattista, Guillermo (2011) Bovine leukosis virus detection in creole colombian breeds using nested-pcr. Acta Agronómica, 60 (4). pp. 312-318. ISSN 2323-01186 Tecnología (ciencias aplicadas) / Technology63 Agricultura y tecnologías relacionadas / AgricultureDiagnóstico molecularganado criolloleucosis bovina enzoótica.Creole cattleenzootic bovine leukosismolecular diagnosticBovine leukosis virus detection in creole colombian breeds using nested-pcrArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL28845-103440-2-PB.pdfapplication/pdf173616https://repositorio.unal.edu.co/bitstream/unal/39993/1/28845-103440-2-PB.pdfa20c20a6e00972589cc66f082ed3a1f6MD51THUMBNAIL28845-103440-2-PB.pdf.jpg28845-103440-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8180https://repositorio.unal.edu.co/bitstream/unal/39993/2/28845-103440-2-PB.pdf.jpg1e424cd084b2680403e1ff06f73b256dMD52unal/39993oai:repositorio.unal.edu.co:unal/399932023-01-26 23:05:11.856Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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