Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)

Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat’s destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its ma...

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Autores:
Salazar Mercado, Seir Antonio
Tipo de recurso:
Article of journal
Fecha de publicación:
2012
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/42776
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/42776
http://bdigital.unal.edu.co/32873/
Palabra clave:
6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cattleya mendelii
cultivo in vitro
desarrollo embrionario
germinación
medio de cultivo
Cattleya mendelii
culture medium
embryonic development
germination
in vitro culture
tetrazolium.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_b380fd3bd4a135aa632f23dba4bf8a0d
oai_identifier_str oai:repositorio.unal.edu.co:unal/42776
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
title Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
spellingShingle Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cattleya mendelii
cultivo in vitro
desarrollo embrionario
germinación
medio de cultivo
Cattleya mendelii
culture medium
embryonic development
germination
in vitro culture
tetrazolium.
title_short Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
title_full Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
title_fullStr Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
title_full_unstemmed Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
title_sort Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
dc.creator.fl_str_mv Salazar Mercado, Seir Antonio
dc.contributor.author.spa.fl_str_mv Salazar Mercado, Seir Antonio
dc.subject.ddc.spa.fl_str_mv 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
topic 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cattleya mendelii
cultivo in vitro
desarrollo embrionario
germinación
medio de cultivo
Cattleya mendelii
culture medium
embryonic development
germination
in vitro culture
tetrazolium.
dc.subject.proposal.spa.fl_str_mv Cattleya mendelii
cultivo in vitro
desarrollo embrionario
germinación
medio de cultivo
Cattleya mendelii
culture medium
embryonic development
germination
in vitro culture
tetrazolium.
description Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat’s destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its marketing. In this study the asymbiotic germination and seedling development of seeds of C. mendelii, in several in vitro culture media were evaluated. Mature capsules were collected. Seed viability with tetrazolium test was done; seeds were disinfected at the same time and planted by the syringe method to evaluate the effect of five growth culture media on the development of C. mendelii after 16 weeks of cultivation. It was found that seed viability was 93%, the highest percentage of germination was found in the culture medium Murashige-Skoog plus coconut water (MS+AC) with significant differences compared to other culture media (P and lt; 0.05, Tukey). This study showed that MS medium supplemented with coconut water and pineapple juice, were more efficient in asymbiotic germination and seedling development of C. mendelii orchids compared with other culture media. It could be an option to reduce the costs generated by using phytohormones.
publishDate 2012
dc.date.issued.spa.fl_str_mv 2012-01-01
dc.date.accessioned.spa.fl_str_mv 2019-06-28T11:10:31Z
dc.date.available.spa.fl_str_mv 2019-06-28T11:10:31Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.issn.spa.fl_str_mv ISSN: 0120-2812
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dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
dc.relation.references.spa.fl_str_mv Salazar Mercado, Seir Antonio (2012) Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae). Acta Agronómica, 61 (1). pp. 69-78. ISSN 0120-2812
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Salazar Mercado, Seir Antonio8810444f-37bf-4194-8b94-25965af491543002019-06-28T11:10:31Z2019-06-28T11:10:31Z2012-01-01ISSN: 0120-2812https://repositorio.unal.edu.co/handle/unal/42776http://bdigital.unal.edu.co/32873/Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat’s destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its marketing. In this study the asymbiotic germination and seedling development of seeds of C. mendelii, in several in vitro culture media were evaluated. Mature capsules were collected. Seed viability with tetrazolium test was done; seeds were disinfected at the same time and planted by the syringe method to evaluate the effect of five growth culture media on the development of C. mendelii after 16 weeks of cultivation. It was found that seed viability was 93%, the highest percentage of germination was found in the culture medium Murashige-Skoog plus coconut water (MS+AC) with significant differences compared to other culture media (P and lt; 0.05, Tukey). This study showed that MS medium supplemented with coconut water and pineapple juice, were more efficient in asymbiotic germination and seedling development of C. mendelii orchids compared with other culture media. It could be an option to reduce the costs generated by using phytohormones.Cattleya mendelii es una orquídea endémica de Colombia de gran valor ornamental que se encuentra en peligro de extinción a causa de la recolección masiva y a la destrucción de sus hábitats naturales por la acción antrópica. El cultivo in vitro es una alternativa para la conservación de esta especie o su comercialización. En esta investigación se evaluaron la germinación asimbiótica y el desarrollo de plántulas de las semillas de orquídeas de la especie C. mendelii, en diferentes medios de cultivos in vitro. Inicialmente se recolectaron cápsulas maduras; posteriormente, se determinó la viabilidad de las semillas con la prueba de Tetrazolio. En forma paralela, las semillas se desinfectaron y sembraron con el método de jeringuilla para evaluar el efecto de cinco medios de cultivo en el desarrollo de C. mendelii después de dieciséis semanas de cultivo. Se encontró que la viabilidad de las semillas fue del 93%. El mejor porcentaje de germinación se halló en el medio de cultivo Murashige-Skoog más agua de coco (MS + AC) con diferencias significativas (P and lt; 0.05, Tukey) con respecto a los demás medios de cultivo. En este estudio se demostró que los medios de cultivos MS suplementados con agua de coco y jugo de piña fueron más eficientes en la germinación asimbiótica y en el desarrollo de plántulas en la orquídea C. mendelii con respecto a los otros medios de cultivo, lo cual los convierte en una opción en la reducción de los costos generados por la utilización de las fitohormonas.application/mswordspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/32463Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaSalazar Mercado, Seir Antonio (2012) Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae). Acta Agronómica, 61 (1). pp. 69-78. ISSN 0120-28126 Tecnología (ciencias aplicadas) / Technology63 Agricultura y tecnologías relacionadas / AgricultureCattleya mendeliicultivo in vitrodesarrollo embrionariogerminaciónmedio de cultivoCattleya mendeliiculture mediumembryonic developmentgerminationin vitro culturetetrazolium.Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL32463-120085-1-PB.docapplication/msword1177088https://repositorio.unal.edu.co/bitstream/unal/42776/1/32463-120085-1-PB.docbf1170290d74fdb2df1f9bf2b39972ccMD5132463-169888-1-PB.docxapplication/vnd.openxmlformats-officedocument.wordprocessingml.document947782https://repositorio.unal.edu.co/bitstream/unal/42776/2/32463-169888-1-PB.docx5d8ee25a58d3622c8fe75101ce764d3aMD5232463-169889-1-PB.pdfapplication/pdf542447https://repositorio.unal.edu.co/bitstream/unal/42776/3/32463-169889-1-PB.pdf2f61cecdbe44e0fb0dc7dbbc80a9090fMD5332463-120086-1-PB.pdfapplication/pdf836059https://repositorio.unal.edu.co/bitstream/unal/42776/4/32463-120086-1-PB.pdf74e04c00b3ec2fbbf23ef0c01efcd69dMD54THUMBNAIL32463-169889-1-PB.pdf.jpg32463-169889-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7345https://repositorio.unal.edu.co/bitstream/unal/42776/5/32463-169889-1-PB.pdf.jpg67febd4df5ffc6b6398780e16e46d403MD5532463-120086-1-PB.pdf.jpg32463-120086-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg5562https://repositorio.unal.edu.co/bitstream/unal/42776/6/32463-120086-1-PB.pdf.jpg9c51e8495bdb10f4bc2a0b239ca7e06aMD56unal/42776oai:repositorio.unal.edu.co:unal/427762023-02-09 23:03:43.331Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co