Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)
Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat’s destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its ma...
- Autores:
-
Salazar Mercado, Seir Antonio
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/42776
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/42776
http://bdigital.unal.edu.co/32873/
- Palabra clave:
- 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cattleya mendelii
cultivo in vitro
desarrollo embrionario
germinación
medio de cultivo
Cattleya mendelii
culture medium
embryonic development
germination
in vitro culture
tetrazolium.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| title |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| spellingShingle |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) 6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture Cattleya mendelii cultivo in vitro desarrollo embrionario germinación medio de cultivo Cattleya mendelii culture medium embryonic development germination in vitro culture tetrazolium. |
| title_short |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| title_full |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| title_fullStr |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| title_full_unstemmed |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| title_sort |
Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae) |
| dc.creator.fl_str_mv |
Salazar Mercado, Seir Antonio |
| dc.contributor.author.spa.fl_str_mv |
Salazar Mercado, Seir Antonio |
| dc.subject.ddc.spa.fl_str_mv |
6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture |
| topic |
6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture Cattleya mendelii cultivo in vitro desarrollo embrionario germinación medio de cultivo Cattleya mendelii culture medium embryonic development germination in vitro culture tetrazolium. |
| dc.subject.proposal.spa.fl_str_mv |
Cattleya mendelii cultivo in vitro desarrollo embrionario germinación medio de cultivo Cattleya mendelii culture medium embryonic development germination in vitro culture tetrazolium. |
| description |
Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat’s destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its marketing. In this study the asymbiotic germination and seedling development of seeds of C. mendelii, in several in vitro culture media were evaluated. Mature capsules were collected. Seed viability with tetrazolium test was done; seeds were disinfected at the same time and planted by the syringe method to evaluate the effect of five growth culture media on the development of C. mendelii after 16 weeks of cultivation. It was found that seed viability was 93%, the highest percentage of germination was found in the culture medium Murashige-Skoog plus coconut water (MS+AC) with significant differences compared to other culture media (P and lt; 0.05, Tukey). This study showed that MS medium supplemented with coconut water and pineapple juice, were more efficient in asymbiotic germination and seedling development of C. mendelii orchids compared with other culture media. It could be an option to reduce the costs generated by using phytohormones. |
| publishDate |
2012 |
| dc.date.issued.spa.fl_str_mv |
2012-01-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-28T11:10:31Z |
| dc.date.available.spa.fl_str_mv |
2019-06-28T11:10:31Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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ISSN: 0120-2812 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/42776 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/32873/ |
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ISSN: 0120-2812 |
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https://repositorio.unal.edu.co/handle/unal/42776 http://bdigital.unal.edu.co/32873/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/acta_agronomica/article/view/32463 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica Acta Agronómica |
| dc.relation.references.spa.fl_str_mv |
Salazar Mercado, Seir Antonio (2012) Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae). Acta Agronómica, 61 (1). pp. 69-78. ISSN 0120-2812 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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Universidad Nacional de Colombia |
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Salazar Mercado, Seir Antonio8810444f-37bf-4194-8b94-25965af491543002019-06-28T11:10:31Z2019-06-28T11:10:31Z2012-01-01ISSN: 0120-2812https://repositorio.unal.edu.co/handle/unal/42776http://bdigital.unal.edu.co/32873/Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat’s destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its marketing. In this study the asymbiotic germination and seedling development of seeds of C. mendelii, in several in vitro culture media were evaluated. Mature capsules were collected. Seed viability with tetrazolium test was done; seeds were disinfected at the same time and planted by the syringe method to evaluate the effect of five growth culture media on the development of C. mendelii after 16 weeks of cultivation. It was found that seed viability was 93%, the highest percentage of germination was found in the culture medium Murashige-Skoog plus coconut water (MS+AC) with significant differences compared to other culture media (P and lt; 0.05, Tukey). This study showed that MS medium supplemented with coconut water and pineapple juice, were more efficient in asymbiotic germination and seedling development of C. mendelii orchids compared with other culture media. It could be an option to reduce the costs generated by using phytohormones.Cattleya mendelii es una orquídea endémica de Colombia de gran valor ornamental que se encuentra en peligro de extinción a causa de la recolección masiva y a la destrucción de sus hábitats naturales por la acción antrópica. El cultivo in vitro es una alternativa para la conservación de esta especie o su comercialización. En esta investigación se evaluaron la germinación asimbiótica y el desarrollo de plántulas de las semillas de orquídeas de la especie C. mendelii, en diferentes medios de cultivos in vitro. Inicialmente se recolectaron cápsulas maduras; posteriormente, se determinó la viabilidad de las semillas con la prueba de Tetrazolio. En forma paralela, las semillas se desinfectaron y sembraron con el método de jeringuilla para evaluar el efecto de cinco medios de cultivo en el desarrollo de C. mendelii después de dieciséis semanas de cultivo. Se encontró que la viabilidad de las semillas fue del 93%. El mejor porcentaje de germinación se halló en el medio de cultivo Murashige-Skoog más agua de coco (MS + AC) con diferencias significativas (P and lt; 0.05, Tukey) con respecto a los demás medios de cultivo. En este estudio se demostró que los medios de cultivos MS suplementados con agua de coco y jugo de piña fueron más eficientes en la germinación asimbiótica y en el desarrollo de plántulas en la orquídea C. mendelii con respecto a los otros medios de cultivo, lo cual los convierte en una opción en la reducción de los costos generados por la utilización de las fitohormonas.application/mswordspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/32463Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaSalazar Mercado, Seir Antonio (2012) Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae). Acta Agronómica, 61 (1). pp. 69-78. ISSN 0120-28126 Tecnología (ciencias aplicadas) / Technology63 Agricultura y tecnologías relacionadas / AgricultureCattleya mendeliicultivo in vitrodesarrollo embrionariogerminaciónmedio de cultivoCattleya mendeliiculture mediumembryonic developmentgerminationin vitro culturetetrazolium.Asymbiotic seed germination and in vitro seedling development of cattleya mendelii dombrain (orchidaceae)Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL32463-120085-1-PB.docapplication/msword1177088https://repositorio.unal.edu.co/bitstream/unal/42776/1/32463-120085-1-PB.docbf1170290d74fdb2df1f9bf2b39972ccMD5132463-169888-1-PB.docxapplication/vnd.openxmlformats-officedocument.wordprocessingml.document947782https://repositorio.unal.edu.co/bitstream/unal/42776/2/32463-169888-1-PB.docx5d8ee25a58d3622c8fe75101ce764d3aMD5232463-169889-1-PB.pdfapplication/pdf542447https://repositorio.unal.edu.co/bitstream/unal/42776/3/32463-169889-1-PB.pdf2f61cecdbe44e0fb0dc7dbbc80a9090fMD5332463-120086-1-PB.pdfapplication/pdf836059https://repositorio.unal.edu.co/bitstream/unal/42776/4/32463-120086-1-PB.pdf74e04c00b3ec2fbbf23ef0c01efcd69dMD54THUMBNAIL32463-169889-1-PB.pdf.jpg32463-169889-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7345https://repositorio.unal.edu.co/bitstream/unal/42776/5/32463-169889-1-PB.pdf.jpg67febd4df5ffc6b6398780e16e46d403MD5532463-120086-1-PB.pdf.jpg32463-120086-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg5562https://repositorio.unal.edu.co/bitstream/unal/42776/6/32463-120086-1-PB.pdf.jpg9c51e8495bdb10f4bc2a0b239ca7e06aMD56unal/42776oai:repositorio.unal.edu.co:unal/427762023-02-09 23:03:43.331Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |