Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)

Documento de tesis de investigación en microbiología.

Autores:: Niño Corredor, Albert Nicolás

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
dc.title.translated.eng.fl_str_mv	Evaluation of the enzymatic activity (ulvano-lyase and laccase) in epiphytic bacteria from macroalgae of the species Ulva lactuca, present in the rocky coastline "La punta de la loma" (Santa Marta - Colombia)
title	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
spellingShingle	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia) Microbiología Bacterias epífitas Bioprospección Ulvano liasas Lacasas
title_short	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
title_full	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
title_fullStr	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
title_full_unstemmed	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
title_sort	Evaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)
dc.creator.fl_str_mv	Niño Corredor, Albert Nicolás
dc.contributor.advisor.none.fl_str_mv	Comba Gonzalez, Natalia
dc.contributor.author.none.fl_str_mv	Niño Corredor, Albert Nicolás
dc.contributor.other.none.fl_str_mv	Montoya Castaño, Dolly
dc.contributor.financer.none.fl_str_mv	Fundación Alejandro Ángel Escobar
dc.contributor.researchgroup.spa.fl_str_mv	Bioprocesos y Bioprospección
dc.subject.ddc.spa.fl_str_mv	Microbiología
topic	Microbiología Bacterias epífitas Bioprospección Ulvano liasas Lacasas
dc.subject.proposal.spa.fl_str_mv	Bacterias epífitas Bioprospección Ulvano liasas Lacasas
description	Documento de tesis de investigación en microbiología.
publishDate	2021
dc.date.issued.none.fl_str_mv	2021-11-20
dc.date.accessioned.none.fl_str_mv	2022-08-11T14:03:33Z
dc.date.available.none.fl_str_mv	2022-08-11T14:03:33Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/81844
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/81844 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Comba Gonzalez, Natalia4c79b98dc81a320f249ef844e70a8441Niño Corredor, Albert Nicolás34c0a5faf0b7b89f30e2e49f9b80ae44Montoya Castaño, DollyFundación Alejandro Ángel EscobarBioprocesos y Bioprospección2022-08-11T14:03:33Z2022-08-11T14:03:33Z2021-11-20https://repositorio.unal.edu.co/handle/unal/81844Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Documento de tesis de investigación en microbiología.fotografías a color, gráficas, ilustraciones, tablasLas enzimas de origen marino presentan características de interés en diversos procesos biotecnológicos, debido a que muchas son activas bajo condiciones fisicoquímicas excepcionales. En este sentido, las comunidades bacterianas epífitas de macroalgas de la especie U. lactuca son una fuente prometedora de enzimas novedosas producidas por las bacterias para metabolizar diferentes sustratos provistos por la superficie macroalgal. En este estudio se realizó el tamizaje funcional de dos enzimas (ulvano-liasas y lacasas) en 90 bacterias epífitas aisladas de la superficie de U. lactuca, recolectadas en La punta de la loma, Santa Marta (Caribe-colombiano). Se obtuvieron un total de 24 aislamientos positivos para actividad lacasa evaluada en tres sustratos (guayacol, 2,6-DMP y ácido tánico), los cuales pertenecieron a los géneros Bacillus, Brevibacterium, Lysinibacillus, Pseudomonas, Shewanella, Vibrio, Alcaligenes y Achromobacter. La evaluación realizada para liasas no arrojó resultados concluyentes respecto a la producción de este tipo de enzimas en los aislamientos de interés. Con el fin de corroborar la presencia de genes codificantes para lacasas en los aislamientos obtenidos, se seleccionó la cepa Achromobacter denitrificans EPI-24, debido a su potencial biorremediador. La confirmación in silico de la presencia de lacasas se verificó mediante el ensamblaje y anotación de su genoma. Los resultados identificaron dos productos génicos (Multicobre Oxidasa y Multicobre Polifenol Oxidasa), los cuales podrían ser responsables de la actividad lacasa detectada en placa, demostrando el potencial de bacterias epífitas de U. lactuca en la producción de enzimas, que podrían presentar propiedades funcionales únicas bajo condiciones más versátiles y eficientes, en comparación con las lacasas convencionales producidas por hongos terrestres. (Texto tomado de la fuente)Marine enzymes have characteristics of interest in various biotechnological processes, because of, many are active under exceptional physicochemical conditions. In this sense, the epiphytic bacterial communities of macroalgae of the species U. lactuca are a promising source of novel enzymes produced by the bacteria to metabolize different substrates provided by the macroalgal surface. In this study, functional screening of two enzymes (ulvane-lyases and lacases) was performed on 90 epiphytic bacteria isolated from the surface of U. lactuca, collected in La punta de la loma, Santa Marta (Colombian Caribbean). A total of 24 isolates were screened positive to laccase activity tested in three substrates (guaiacol, 2,6-DMP and tannic acid), belonging to the genera Bacillus, Brevibacterium, Lysinibacillus, Pseudomonas, Shewanella, Vibrio, Alcaligenes and Achromobacter. The assess for lyases did not show conclusive results regarding the production of this type of enzymes in the isolates of interest. In order to corroborate the presence of laccase-coding genes in the isolates obtained, the Achromobacter denitrificans EPI-24 strain was selected due to its bioremediation potential. The in silico confirmation of the presence of laccase was verified by the assembly and annotation of its genome. The results identified two gene products (Multicopper Oxidase and Multicopper Polyphenol Oxidase), which could be responsible for the laccase activity detected in plate, demonstrating the potential of epiphytic bacteria of U. lactuca in the production of enzymes, which could present unique functional properties under more versatile and efficient conditions, compared to conventional lacases produced by terrestrial fungi. (Text taken from the source)MaestríaMagister en Ciencias - MicrobiologíaBiotecnologíaxx, 118 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaInstituto de Biotecnología (IBUN)Facultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede BogotáMicrobiologíaBacterias epífitasBioprospecciónUlvano liasasLacasasEvaluación de la actividad enzimática (ulvano-liasa y lacasa) en bacterias epífitas de macroalgas de la especie Ulva lactuca, presentes en el litoral rocoso “La punta de la loma” (Santa Marta - Colombia)Evaluation of the enzymatic activity (ulvano-lyase and laccase) in epiphytic bacteria from macroalgae of the species Ulva lactuca, present in the rocky coastline "La punta de la loma" (Santa Marta - Colombia)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbdel-Rahman, M. A., Tashiro, Y., & Sonomoto, K. (2013). 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Journal of Marine Research, 203.InvestigadoresORIGINAL1010203407.2021.pdf1010203407.2021.pdftesis de Maestría en Ciencias - Microbiologíaapplication/pdf4013785https://repositorio.unal.edu.co/bitstream/unal/81844/3/1010203407.2021.pdf4e57f5a0617d9a8c7bf70030ed6e21e7MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81844/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1010203407.2021.pdf.jpg1010203407.2021.pdf.jpgGenerated Thumbnailimage/jpeg5235https://repositorio.unal.edu.co/bitstream/unal/81844/5/1010203407.2021.pdf.jpg8a2f431eec9add3753126d3d69d3d1d4MD55unal/81844oai:repositorio.unal.edu.co:unal/818442024-08-08 23:10:40.176Repositorio Institucional Universidad Nacional de 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