Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo
ilustraciones, diagramas, fotografías a color
- Autores:
-
Daza Leon, Camila
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84415
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Salud animal
Pollos de engorde
Cattle - health
Broilers (poultry)
Aves de corral
Propóleo
Proteínas de unión (TJ)
Salud intestinal
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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oai:repositorio.unal.edu.co:unal/84415 |
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| dc.title.translated.eng.fl_str_mv |
Evaluation of the expression of genes associated with intestinal integrity and the modulation of the immune response in broilers supplemented with propolis |
| title |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| spellingShingle |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo 630 - Agricultura y tecnologías relacionadas::636 - Producción animal Salud animal Pollos de engorde Cattle - health Broilers (poultry) Aves de corral Propóleo Proteínas de unión (TJ) Salud intestinal |
| title_short |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| title_full |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| title_fullStr |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| title_full_unstemmed |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| title_sort |
Evaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleo |
| dc.creator.fl_str_mv |
Daza Leon, Camila |
| dc.contributor.advisor.none.fl_str_mv |
Gomez Ramirez, Arlen Patricia Ramirez-Nieto, Gloria Consuelo |
| dc.contributor.author.none.fl_str_mv |
Daza Leon, Camila |
| dc.contributor.researchgroup.spa.fl_str_mv |
Medicina Aviar y Producción Avícola |
| dc.contributor.orcid.spa.fl_str_mv |
0000-0002-7221-8311 |
| dc.contributor.researchgate.spa.fl_str_mv |
Daza Leon, Camila |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal |
| topic |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal Salud animal Pollos de engorde Cattle - health Broilers (poultry) Aves de corral Propóleo Proteínas de unión (TJ) Salud intestinal |
| dc.subject.lemb.spa.fl_str_mv |
Salud animal Pollos de engorde |
| dc.subject.lemb.eng.fl_str_mv |
Cattle - health Broilers (poultry) |
| dc.subject.proposal.spa.fl_str_mv |
Aves de corral Propóleo Proteínas de unión (TJ) Salud intestinal |
| description |
ilustraciones, diagramas, fotografías a color |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-08-02T15:37:32Z |
| dc.date.available.none.fl_str_mv |
2023-08-02T15:37:32Z |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
DataPaper Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84415 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84415 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Frontiers in Veterinary Science, 7, 572142. https://doi.org/10.3389/fvets.2020.572142 Eyng, C., Murakami, A. E., Santos, T. C., Silveira, T. G. v., Pedroso, R. B., & Lourenço, D. A. L. (2014). Immune Responses in Broiler Chicks Fed Propolis Extraction Residue-supplemented Diets. Asian-Australasian Journal of Animal Sciences, 28(1), 135-142. https://doi.org/10.5713/ajas.14.0066 Fasina, Y. O., Classen, H. L., Garlich, J. D., Black, B. L., Ferket, P. R., Uni, Z., & Olkowski, A. A. (2006). Response of Turkey Poults to Soybean Lectin Levels Typically Encountered in Commercial Diets. 2. Effect on Intestinal Development and Lymphoid Organs. https://doi.org/https://doi.org/10.1093/ps/85.5.870 Fata, G. La, Weber, P., & Mohajeri, M. H. (2018). Probiotics and the Gut Immune System: Indirect Regulation. Probiotics and Antimicrobial Proteins, 10(1), 11. https://doi.org/10.1007/S12602-017-9322-6 Fischer, G., Paulino, N., Marcucci, M. C., Siedler, B. S., Munhoz, L. S., Finger, P. F., Vargas, G. D., Hübner, S. O., Vidor, T., & Roehe, P. M. (2010). Green propolis phenolic compounds act as vaccine adjuvants, improving humoral and cellular responses in mice inoculated with inactivated vaccines. Memórias do Instituto Oswaldo Cruz, 105(7), 908-913. https://doi.org/10.1590/S0074-02762010000700012 Forder, R. E. A., Howarth, G. S., Tivey, D. R., & Hughes, R. J. (2007). Bacterial modulation of small intestinal goblet cells and mucin composition during early posthatch development of poultry. Poultry Science, 86(11), 2396-2403. https://doi.org/10.3382/ps.2007-00222 |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gomez Ramirez, Arlen Patriciaf5cc44fb42ba93419c8ed45b395b3190Ramirez-Nieto, Gloria Consuelo2bde2e176bb7a2cb8f6cecb04a010271Daza Leon, Camila6dcff9071cd2bfc97d77dee7db403709Medicina Aviar y Producción Avícola0000-0002-7221-8311Daza Leon, Camila2023-08-02T15:37:32Z2023-08-02T15:37:32Z2023https://repositorio.unal.edu.co/handle/unal/84415Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías a colorComo alternativa a la prohibición mundial de los antibióticos utilizados como promotores del crecimiento (AGP), los aditivos nutricionales, como el propóleo, buscan mejorar la salud intestinal de los animales. Con base en lo anterior, el objetivo de este estudio fue evaluar el efecto de la suplementación con propóleo en la dieta de pollos de engorde. Se alimentaron 450 pollos de engorde Ross 308 AP con una dieta basal (BD) durante todo el periodo experimental. Las aves se distribuyeron aleatoriamente en cinco grupos en el día 14: control negativo sin antibióticos ni propóleo (AGP-), control positivo con 500 ppm de bacitracina de zinc como AGP (AGP+) y tres grupos suplementados con 150, 300 y 450 ppm de propóleo. Cada grupo incluía seis réplicas de 15 aves cada una. La concentración de propóleo se incrementó de los días 22 al 42 a 300, 600 y 900 ppm, y se incluyó un 10% de soya cruda como desafío en todos los grupos durante el mismo periodo. A los 21 y 42 días se realizó el análisis de parámetros productivos, morfometría intestinal, histología de órganos inmunes y cuantificación relativa de los genes asociados a la integridad epitelial e inmunidad mediante qPCR. Los grupos con mayor peso fueron los que consumieron dietas que incluían 150 ppm (21 d) y 900 ppm (42 d) de propóleo en comparación con todos los tratamientos. La puntuación más baja de ISI se encontró en 300 (21 d) y 600 ppm (42 d). Se observó un menor grado de lesión en el sistema digestivo con la inclusión de 300 ppm (21 d) y 900 ppm (42 d). Se detectó incremento de la expresión de zónula occludens-1 (ZO-1) en yeyuno de pollos de engorde suplementados con 150 y 300 ppm a los 21 días. También se evidenció la regulación negativa de TGF- en íleon en todos los niveles de inclusión de propóleo a los 42 días en comparación con AGP+ y AGP-. Los efectos beneficiosos se evidenciaron a niveles de inclusión de 150 ppm en el alimento iniciador y 900 ppm en el finalizador. la inclusión de propóleos colombianos puede mejorar el rendimiento productivo, los parámetros fisiológicos e inmunes, y la expresión de genes asociados a la integridad intestinal. (Texto tomado de la fuente)Nutritional additives such as propolis seek to improve intestinal health as an alternative to the global ban on in-feed antibiotics used as growth promoters (AGP). The objective of this study was to evaluate the effect of propolis supplementation in diet of broilers. Four hundred and fifty straight-run Ross 308 AP broilers were fed with a basal diet (BD) throughout the whole experimental period. Birds were randomly distributed into five groups at day 14: negative control without antibiotics nor propolis (AGP-), positive control 500 ppm of Zinc Bacitracin as growth promoter (AGP+), and three groups supplemented with 150, 300, and 450 ppm of propolis. Every group included six replicates of 15 birds each. Propolis concentration was increased from day 22 to 42, in experimental groups to 300, 600, and 900 ppm of propolis, and 10% of raw soybean was included as a challenge in all groups during the same period. Analysis of productive parameters, intestinal morphometry, and relative quantification of genes associated with epithelial integrity by qPCR were performed at 21 and 42 days. The groups with the greatest weights were those that consumed diets including 150 (21 d) and 900 ppm (42 d) of propolis compared with all treatments. The lowest score of ISI was found at 300 (21 d) and 600 ppm (42 d). A lower degree of injury in the digestive system was seen with the inclusion of 300 ppm (21 d) and 900 ppm (42 d). Up-regulation of zonula occludens-1 (ZO-1) was observed in jejunum of broilers supplemented with 150 and 300 ppm at 21 d. Up-regulation of ZO-1 and TGF- was also evidenced in the ileum at all propolis inclusion levels at 42 d-old compared to AGP+ and AGP-. The beneficial effects were evidenced at inclusion levels of 150 ppm in the starter and 900 ppm in the finisher. According to the results, the Colombian propolis inclusion can improve productive performance, physiological parameters, and the expression of genes associated with intestinal integrity.MaestríaMagíster en Salud Animal o Magíster en Producción AnimalMicrobiología e inmunologíaxix, 95 páginasapplication/pdfspa630 - Agricultura y tecnologías relacionadas::636 - Producción animalSalud animalPollos de engordeCattle - healthBroilers (poultry)Aves de corralPropóleoProteínas de unión (TJ)Salud intestinalEvaluación de la expresión de genes asociados con la integridad intestinal y la modulación de la respuesta inmune en pollos de engorde suplementados con propóleoEvaluation of the expression of genes associated with intestinal integrity and the modulation of the immune response in broilers supplemented with propolisTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionDataPaperTexthttp://purl.org/redcol/resource_type/TMBogotá - Medicina Veterinaria y de Zootecnia - Maestría en Salud y Producción AnimalFacultad de Medicina Veterinaria y de ZootecniaBogotá,ColombiaUniversidad Nacional de Colombia - Sede BogotáGalal, A., Abd El -Motaal, A. 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Poultry Science, 86(11), 2396-2403. https://doi.org/10.3382/ps.2007-00222EstudiantesInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84415/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1026300192.2023.pdf1026300192.2023.pdfTesis de Maestría en Salud Animalapplication/pdf2545089https://repositorio.unal.edu.co/bitstream/unal/84415/2/1026300192.2023.pdfe9480d49a071765a3718aed5ddaedb02MD52THUMBNAIL1026300192.2023.pdf.jpg1026300192.2023.pdf.jpgGenerated Thumbnailimage/jpeg5905https://repositorio.unal.edu.co/bitstream/unal/84415/3/1026300192.2023.pdf.jpg5a5b6126b3b8b97b9c3555a3ff532c27MD53unal/84415oai:repositorio.unal.edu.co:unal/844152023-08-15 23:03:57.255Repositorio Institucional Universidad Nacional de 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