Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch

Bacillus cereus is a food contaminant anda known human pathogen that can causeemetic and diarrheal syndromes. In this studywe evaluated the presence of toxigenic B.cereus by multiplex PCR directly in dietarycomplement for children and cassava starchsamples collected on Medellin, Colombia.Of 75 dieta...

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Autores:
Sánchez, Jnnifer A.
Correa, Margarita M.
Aceves Dies, Ángel E.
Castañeda Sandoval, Laura M.
Tipo de recurso:
Article of journal
Fecha de publicación:
2014
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/66319
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/66319
http://bdigital.unal.edu.co/67343/
Palabra clave:
54 Química y ciencias afines / Chemistry
Bacillus cereus
enterotoxins
emetic toxin
dried foods
Multiplex PCR.
Bacillus cereus
enterotoxinas
toxina emética
alimentos pulverizados
PCR múltiple.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_b04b68b41559b7e366974b568823e27e
oai_identifier_str oai:repositorio.unal.edu.co:unal/66319
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
title Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
spellingShingle Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
54 Química y ciencias afines / Chemistry
Bacillus cereus
enterotoxins
emetic toxin
dried foods
Multiplex PCR.
Bacillus cereus
enterotoxinas
toxina emética
alimentos pulverizados
PCR múltiple.
title_short Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
title_full Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
title_fullStr Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
title_full_unstemmed Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
title_sort Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
dc.creator.fl_str_mv Sánchez, Jnnifer A.
Correa, Margarita M.
Aceves Dies, Ángel E.
Castañeda Sandoval, Laura M.
dc.contributor.author.spa.fl_str_mv Sánchez, Jnnifer A.
Correa, Margarita M.
Aceves Dies, Ángel E.
Castañeda Sandoval, Laura M.
dc.subject.ddc.spa.fl_str_mv 54 Química y ciencias afines / Chemistry
topic 54 Química y ciencias afines / Chemistry
Bacillus cereus
enterotoxins
emetic toxin
dried foods
Multiplex PCR.
Bacillus cereus
enterotoxinas
toxina emética
alimentos pulverizados
PCR múltiple.
dc.subject.proposal.spa.fl_str_mv Bacillus cereus
enterotoxins
emetic toxin
dried foods
Multiplex PCR.
Bacillus cereus
enterotoxinas
toxina emética
alimentos pulverizados
PCR múltiple.
description Bacillus cereus is a food contaminant anda known human pathogen that can causeemetic and diarrheal syndromes. In this studywe evaluated the presence of toxigenic B.cereus by multiplex PCR directly in dietarycomplement for children and cassava starchsamples collected on Medellin, Colombia.Of 75 dietary complement for childrensamples evaluated, 70.7% were contaminatedwith toxigenic B. cereus and four differenttoxigenic consortia were detected: I: nheA,hblC, cytK (9.8%), II: nheA, hblC (2%),III: hblC, cytK (41.2%), IV: hblC (47%).Of 75 cassava starch samples, 44% werecontaminated with toxigenic B. cereus andfour different toxigenic consortia weredetermined: I: nheA, hblC, cytK (48.5%),II: nheA, hblC, cytK, cesB (3%), III: hblC,cytK (30.3%), IV: hblC (18.2%). In general,in dietary complement for children onlyenterotoxigenic consortia were detectedwhile in cassava starch the enterotoxigenicconsortia predominated over the emetic.Multiplex PCR was useful to detect toxigenicB. cereus contamination allowing directand simultaneous detection of all toxingenes in foods. This study is the first inColombia to evaluate toxigenic B. cereus,providing information of importance formicrobiological risk evaluation in driedfoods.
publishDate 2014
dc.date.issued.spa.fl_str_mv 2014-05-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T01:54:06Z
dc.date.available.spa.fl_str_mv 2019-07-03T01:54:06Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.issn.spa.fl_str_mv ISSN: 2357-3791
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/66319
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/67343/
identifier_str_mv ISSN: 2357-3791
url https://repositorio.unal.edu.co/handle/unal/66319
http://bdigital.unal.edu.co/67343/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/rcolquim/article/view/53425
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química
Revista Colombiana de Química
dc.relation.references.spa.fl_str_mv Sánchez, Jnnifer A. and Correa, Margarita M. and Aceves Dies, Ángel E. and Castañeda Sandoval, Laura M. (2014) Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch. Revista Colombiana de Química, 43 (2). pp. 5-9. ISSN 2357-3791
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Química
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/66319/1/53425-262418-1-SM.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Sánchez, Jnnifer A.115578e7-419a-40b9-b306-8a82744f4d4d300Correa, Margarita M.c770bf06-badb-4f3f-a23b-32a7ace9e7de300Aceves Dies, Ángel E.c9b33e8b-19a0-482c-81b1-d412c77b1b45300Castañeda Sandoval, Laura M.6a96108f-770f-46b5-b198-9dc0c459df0b3002019-07-03T01:54:06Z2019-07-03T01:54:06Z2014-05-01ISSN: 2357-3791https://repositorio.unal.edu.co/handle/unal/66319http://bdigital.unal.edu.co/67343/Bacillus cereus is a food contaminant anda known human pathogen that can causeemetic and diarrheal syndromes. In this studywe evaluated the presence of toxigenic B.cereus by multiplex PCR directly in dietarycomplement for children and cassava starchsamples collected on Medellin, Colombia.Of 75 dietary complement for childrensamples evaluated, 70.7% were contaminatedwith toxigenic B. cereus and four differenttoxigenic consortia were detected: I: nheA,hblC, cytK (9.8%), II: nheA, hblC (2%),III: hblC, cytK (41.2%), IV: hblC (47%).Of 75 cassava starch samples, 44% werecontaminated with toxigenic B. cereus andfour different toxigenic consortia weredetermined: I: nheA, hblC, cytK (48.5%),II: nheA, hblC, cytK, cesB (3%), III: hblC,cytK (30.3%), IV: hblC (18.2%). In general,in dietary complement for children onlyenterotoxigenic consortia were detectedwhile in cassava starch the enterotoxigenicconsortia predominated over the emetic.Multiplex PCR was useful to detect toxigenicB. cereus contamination allowing directand simultaneous detection of all toxingenes in foods. This study is the first inColombia to evaluate toxigenic B. cereus,providing information of importance formicrobiological risk evaluation in driedfoods.Bacillus cereus es un contaminante de alimentosconocido por ser patogénico para los humanos,causando síndromes de vómito y diarrea.En este estudio se evaluó la presencia de B.cereus toxigénicos utilizando PCR múltipledirectamente en complementos dietariospara niños y en almidón de yuca colectadosen Medellín, Colombia. De 75 muestras decomplemento dietario para niños, 70,7%estuvieron contaminadas con B. cereustoxigénicos y se detectaron cuatro diferentesconsorcios toxigénicos: I: nheA, hblC, cytK(9,8%), II: nheA, hblC (2%), III: hblC, cytK(41.2%), IV: hblC (47%). De 75 muestras dealmidón de yuca, 44% estuvieron contaminadascon B. cereus toxigénicos y se determinaroncuatro diferentes consorcios toxigénicos: I:nheA, hblC, cytK (48.5%), II: nheA, hblC, cytK,cesB (3%), III: hblC, cytK (30,3%), IV: hblC(18.2%). En general, en los complementosdietarios para niños sólo se detectaronconsorcios enterotoxigénicos, mientras que enel almidón los consorcios enterotoxigénicospredominaron sobre el emético. La PCRmúltiple fue de utilidad para detectarcontaminación con B. cereus toxigénicospermitiendo la detección directa y simultáneade todos los genes tóxicos en los alimentos. Esteestudio es el primero en Colombia en evaluar B.cereus toxigénicos y proporciona informaciónimportante para la evaluación de riesgosmicrobiológicos en los alimentos pulverizadosapplication/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Químicahttps://revistas.unal.edu.co/index.php/rcolquim/article/view/53425Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de QuímicaRevista Colombiana de QuímicaSánchez, Jnnifer A. and Correa, Margarita M. and Aceves Dies, Ángel E. and Castañeda Sandoval, Laura M. (2014) Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch. Revista Colombiana de Química, 43 (2). pp. 5-9. ISSN 2357-379154 Química y ciencias afines / ChemistryBacillus cereusenterotoxinsemetic toxindried foodsMultiplex PCR.Bacillus cereusenterotoxinastoxina eméticaalimentos pulverizadosPCR múltiple.Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starchArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL53425-262418-1-SM.pdfapplication/pdf161086https://repositorio.unal.edu.co/bitstream/unal/66319/1/53425-262418-1-SM.pdfaa8ccf146d2bdaf5d6088a324f0df302MD51THUMBNAIL53425-262418-1-SM.pdf.jpg53425-262418-1-SM.pdf.jpgGenerated Thumbnailimage/jpeg8891https://repositorio.unal.edu.co/bitstream/unal/66319/2/53425-262418-1-SM.pdf.jpg6566f58d78e3621641e6ad2cc990c513MD52unal/66319oai:repositorio.unal.edu.co:unal/663192023-05-24 23:03:06.29Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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