Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch

Bacillus cereus is a food contaminant anda known human pathogen that can causeemetic and diarrheal syndromes. In this studywe evaluated the presence of toxigenic B.cereus by multiplex PCR directly in dietarycomplement for children and cassava starchsamples collected on Medellin, Colombia.Of 75 dieta...

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Autores:
Sánchez, Jnnifer A.
Correa, Margarita M.
Aceves Dies, Ángel E.
Castañeda Sandoval, Laura M.
Tipo de recurso:
Article of journal
Fecha de publicación:
2014
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/66319
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/66319
http://bdigital.unal.edu.co/67343/
Palabra clave:
54 Química y ciencias afines / Chemistry
Bacillus cereus
enterotoxins
emetic toxin
dried foods
Multiplex PCR.
Bacillus cereus
enterotoxinas
toxina emética
alimentos pulverizados
PCR múltiple.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
Description
Summary:Bacillus cereus is a food contaminant anda known human pathogen that can causeemetic and diarrheal syndromes. In this studywe evaluated the presence of toxigenic B.cereus by multiplex PCR directly in dietarycomplement for children and cassava starchsamples collected on Medellin, Colombia.Of 75 dietary complement for childrensamples evaluated, 70.7% were contaminatedwith toxigenic B. cereus and four differenttoxigenic consortia were detected: I: nheA,hblC, cytK (9.8%), II: nheA, hblC (2%),III: hblC, cytK (41.2%), IV: hblC (47%).Of 75 cassava starch samples, 44% werecontaminated with toxigenic B. cereus andfour different toxigenic consortia weredetermined: I: nheA, hblC, cytK (48.5%),II: nheA, hblC, cytK, cesB (3%), III: hblC,cytK (30.3%), IV: hblC (18.2%). In general,in dietary complement for children onlyenterotoxigenic consortia were detectedwhile in cassava starch the enterotoxigenicconsortia predominated over the emetic.Multiplex PCR was useful to detect toxigenicB. cereus contamination allowing directand simultaneous detection of all toxingenes in foods. This study is the first inColombia to evaluate toxigenic B. cereus,providing information of importance formicrobiological risk evaluation in driedfoods.