Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes

Ilustraciones, tablas

Autores:
Osorio Rueda, Juan Gabriel
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/82123
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/82123
https://repositorio.unal.edu.co/
Palabra clave:
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Endosimbiontes
Wolbachia
Rickettsia
Hormiga
Infección
Endosymbionts
Wolbachia
Rickettsia
Ant
Infection
Rights
openAccess
License
Reconocimiento 4.0 Internacional
id UNACIONAL2_ace4d988e887ffc8d359d01311bd31ae
oai_identifier_str oai:repositorio.unal.edu.co:unal/82123
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
dc.title.translated.eng.fl_str_mv Prevalence and infection status of endosymbionts Wolbachia spp. and Rickettsia spp. in populations of Atta cephalotes
title Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
spellingShingle Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Endosimbiontes
Wolbachia
Rickettsia
Hormiga
Infección
Endosymbionts
Wolbachia
Rickettsia
Ant
Infection
title_short Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
title_full Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
title_fullStr Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
title_full_unstemmed Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
title_sort Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes
dc.creator.fl_str_mv Osorio Rueda, Juan Gabriel
dc.contributor.advisor.none.fl_str_mv Muñoz Flórez, Jaime Eduardo
dc.contributor.author.none.fl_str_mv Osorio Rueda, Juan Gabriel
dc.contributor.educationalvalidator.none.fl_str_mv Montoya Lerma, James
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Investigación en Diversidad Biológica
dc.subject.ddc.spa.fl_str_mv 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
topic 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Endosimbiontes
Wolbachia
Rickettsia
Hormiga
Infección
Endosymbionts
Wolbachia
Rickettsia
Ant
Infection
dc.subject.proposal.spa.fl_str_mv Endosimbiontes
Wolbachia
Rickettsia
Hormiga
Infección
dc.subject.proposal.eng.fl_str_mv Endosymbionts
Wolbachia
Rickettsia
Ant
Infection
description Ilustraciones, tablas
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-08-25T21:09:36Z
dc.date.available.none.fl_str_mv 2022-08-25T21:09:36Z
dc.date.issued.none.fl_str_mv 2022-08-24
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
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status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/82123
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/82123
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Palmira - Ciencias Agropecuarias - Maestría en Ciencias Biológicas
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias Agropecuarias
dc.publisher.place.spa.fl_str_mv Palmira Valle del Cauca, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Palmira
institution Universidad Nacional de Colombia
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spelling Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Muñoz Flórez, Jaime Eduardo21cc0d0661318d1e84ac9a225a0fbdc5Osorio Rueda, Juan Gabrielf3d6ee7967a294cefb317135c3218e09600Montoya Lerma, JamesGrupo de Investigación en Diversidad Biológica2022-08-25T21:09:36Z2022-08-25T21:09:36Z2022-08-24https://repositorio.unal.edu.co/handle/unal/82123Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, tablasLas bacterias simbiontes habitan una gran parte de los artrópodos en el mundo, incluidas las hormigas (Hymenoptera: Formicidae). Algunos de estos endosimbiontes manipulan la reproducción, como los géneros Rickettsia y Wolbachia, destacándose en muchos aspectos de la vida del huésped. Aunque tienen un potencial biotecnológico en el manejo integrado de plagas, se desconoce el estatus de infección y prevalencia en el ciclo de vida de especies de alto impacto económico como la hormiga arriera Atta cephalotes. Se evaluaron un conjunto de cebadores específicos para Rickettsia, gen Rb, y Wolbachia, gen wsp, empleando una PCR anidada con la finalidad de detectar estos niveles de infección en los estadios larva, pupa y adulto de muestras colectadas en diferentes nidos de esta hormiga arriera en el Valle del Cauca. Encontramos para una banda de 900 pb que la infección por Rickettsia (27.7% de los nidos) es relativamente común en comparación con Wolbachia quien no fue detectada en este estudio. Se identificó una probable especie procariota endosimbionte Rickettsia sp. y a pesar del desconocimiento de los efectos fisiológicos de este microrganismo sobre la hormiga arriera, comprende uno de los primeros pasos en Colombia para profundizar en la biología y ecología de esta fascinante interacción. (Texto tomado de la fuente)Symbiotic bacteria inhabit a large part of the world's arthropods, including ants (Hymenoptera: Formicidae). Some of these endosymbionts manipulate reproduction, such as the Rickettsia and Wolbachia genera, excelling in many aspects of host life. Although they have biotechnological potential in integrated pest management, the infection status and prevalence in the life cycle of species with a high economic impact, such as the leafcutter ant Atta cephalotes, are unknown. A set of specific primers for Rickettsia, Rb gene, and Wolbachia, wsp gene, were evaluated using a nested PCR to detect these levels of infection in the larval, pupal, and adult stages of samples collected in different nests of this leafcutter ant. in the Valle del Cauca. We found for a 900 bp band that Rickettsia infection (27.7% of nests) is relatively common compared to Wolbachia which was not detected in this study. A probable endosymbiotic prokaryotic species Rickettsia sp. and despite the lack of knowledge of the physiological effects of this microorganism on the ant, it comprises one of the first steps in Colombia to deepen the biology and ecology of this fascinating interaction.MaestríaMagíster en Ciencias BiológicasSe evaluaron un conjunto de cebadores específicos para Rickettsia, gen Rb, y Wolbachia, gen wsp, empleando una PCR anidada con la finalidad de detectar estos niveles de infección en los estadios larva, pupa y adulto de muestras colectadas en diferentes nidos de esta hormiga arriera en el Valle del Cauca.xiv, 43 páginas + anexosapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ciencias Agropecuarias - Maestría en Ciencias BiológicasFacultad de Ciencias AgropecuariasPalmira Valle del Cauca, ColombiaUniversidad Nacional de Colombia - Sede Palmira630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesEndosimbiontesWolbachiaRickettsiaHormigaInfecciónEndosymbiontsWolbachiaRickettsiaAntInfectionPrevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotesPrevalence and infection status of endosymbionts Wolbachia spp. and Rickettsia spp. in populations of Atta cephalotesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAdams, R. 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Biological Reviews of the Cambridge Philosophical Society, 90(1), 89–111. https://doi.org/10.1111/brv.12098EstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unal.edu.co/bitstream/unal/82123/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL1093738930.2022.pdf1093738930.2022.pdfTesis de Maestría en Ciencias Biológicasapplication/pdf3140101https://repositorio.unal.edu.co/bitstream/unal/82123/2/1093738930.2022.pdf26715114b7f7ad6f97f66e9a5ea0583dMD52THUMBNAIL1093738930.2022.pdf.jpg1093738930.2022.pdf.jpgGenerated Thumbnailimage/jpeg4970https://repositorio.unal.edu.co/bitstream/unal/82123/3/1093738930.2022.pdf.jpg63c3e62389597150db721c8044b21368MD53unal/82123oai:repositorio.unal.edu.co:unal/821232023-08-08 23:03:53.062Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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