Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morp...
- Autores:
-
Crespo-Muñoz, Sergio
Rivera-Peña, Mariela
Rosero-Alpala, Deisy Alexandra
Muñoz-Florez, Jaime Eduardo
Rao, Idupulapati M.
Muñoz-Florez, Ligia Carmenza
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/68090
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/68090
http://bdigital.unal.edu.co/69123/
- Palabra clave:
- 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Abiotic stress
high temperature
humidity content
Fabaceae
Rhizobiaceae
inoculation
mutation induction.
Altas temperaturas
estrés abiótico
contenido de humedad
Fabaceae
Rhizobiaceae
inoculación
inducción de mutaciones.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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|
dc.title.spa.fl_str_mv |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
title |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
spellingShingle |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia 55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture Abiotic stress high temperature humidity content Fabaceae Rhizobiaceae inoculation mutation induction. Altas temperaturas estrés abiótico contenido de humedad Fabaceae Rhizobiaceae inoculación inducción de mutaciones. |
title_short |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
title_full |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
title_fullStr |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
title_full_unstemmed |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
title_sort |
Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia |
dc.creator.fl_str_mv |
Crespo-Muñoz, Sergio Rivera-Peña, Mariela Rosero-Alpala, Deisy Alexandra Muñoz-Florez, Jaime Eduardo Rao, Idupulapati M. Muñoz-Florez, Ligia Carmenza |
dc.contributor.author.spa.fl_str_mv |
Crespo-Muñoz, Sergio Rivera-Peña, Mariela Rosero-Alpala, Deisy Alexandra Muñoz-Florez, Jaime Eduardo Rao, Idupulapati M. Muñoz-Florez, Ligia Carmenza |
dc.subject.ddc.spa.fl_str_mv |
55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture |
topic |
55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture Abiotic stress high temperature humidity content Fabaceae Rhizobiaceae inoculation mutation induction. Altas temperaturas estrés abiótico contenido de humedad Fabaceae Rhizobiaceae inoculación inducción de mutaciones. |
dc.subject.proposal.spa.fl_str_mv |
Abiotic stress high temperature humidity content Fabaceae Rhizobiaceae inoculation mutation induction. Altas temperaturas estrés abiótico contenido de humedad Fabaceae Rhizobiaceae inoculación inducción de mutaciones. |
description |
Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morpho-physiological parameters on pollen viability and quantity in 123 mutant lines M5 of P. acutifolius and parental genotypes, by processing micrographs of pollen stained with acetocarmine. The experiment was conducted under greenhouse conditions and the experimental design was a split-split plot with three replicates; in addition, the factors evaluated were as follows: soil moisture conditions, inoculation with Bradyrhizobium sp. or Rhizobium tropici, and the evaluated lines (6 mutants and 2 non-mutants). The experiment was carried out maintaining night temperature above 25°C. There were no significant differences among treatments, and there was a positive correlation of pollen viability with other variables. |
publishDate |
2018 |
dc.date.issued.spa.fl_str_mv |
2018-04-01 |
dc.date.accessioned.spa.fl_str_mv |
2019-07-03T05:49:15Z |
dc.date.available.spa.fl_str_mv |
2019-07-03T05:49:15Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.issn.spa.fl_str_mv |
ISSN: 2323-0118 |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/68090 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/69123/ |
identifier_str_mv |
ISSN: 2323-0118 |
url |
https://repositorio.unal.edu.co/handle/unal/68090 http://bdigital.unal.edu.co/69123/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/57704 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica Acta Agronómica |
dc.relation.references.spa.fl_str_mv |
Crespo-Muñoz, Sergio and Rivera-Peña, Mariela and Rosero-Alpala, Deisy Alexandra and Muñoz-Florez, Jaime Eduardo and Rao, Idupulapati M. and Muñoz-Florez, Ligia Carmenza (2018) Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia. Acta Agronómica, 67 (2). pp. 319-325. ISSN 2323-0118 |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia - Sede Palmira |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/68090/1/57704-367292-6-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/68090/2/57704-367292-6-PB.pdf.jpg |
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Repositorio Institucional Universidad Nacional de Colombia |
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repositorio_nal@unal.edu.co |
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1814089927168622592 |
spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Crespo-Muñoz, Sergio531e3d75-f05c-4a63-908d-863f9f4b6333300Rivera-Peña, Marielac39e935a-9b75-4e5a-9f71-17aaa522f6f6300Rosero-Alpala, Deisy Alexandrafe23c423-18b5-48e0-b6da-63afd18b144c300Muñoz-Florez, Jaime Eduardode00a3e9-9ff7-4c8e-bcae-d5699ea4c1d4300Rao, Idupulapati M.ea5cd341-c7de-4996-8fe7-ebbd5dd68f3a300Muñoz-Florez, Ligia Carmenza4eaf8368-1994-410d-9963-2a13efea205c3002019-07-03T05:49:15Z2019-07-03T05:49:15Z2018-04-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/68090http://bdigital.unal.edu.co/69123/Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morpho-physiological parameters on pollen viability and quantity in 123 mutant lines M5 of P. acutifolius and parental genotypes, by processing micrographs of pollen stained with acetocarmine. The experiment was conducted under greenhouse conditions and the experimental design was a split-split plot with three replicates; in addition, the factors evaluated were as follows: soil moisture conditions, inoculation with Bradyrhizobium sp. or Rhizobium tropici, and the evaluated lines (6 mutants and 2 non-mutants). The experiment was carried out maintaining night temperature above 25°C. There were no significant differences among treatments, and there was a positive correlation of pollen viability with other variables.El fríjol tépari, Phaseolus acutifolius A. Gray destaca por su tolerancia al estrés por sequía y altas temperaturas, sin embargo, presenta baja diversidad genética en sus formas cultivadas. Por lo que se inició un programa de inducción de mutaciones. El objetivo de este estudio fue establecer el efecto de diferentes parámetros agro-morfofisiológicos asociados sobre la viabilidad y cantidad del polen en 123 líneas mutantes de la quinta generación M5 de P. acutifolius y genotipos parentales, por medio del procesamiento de microfotografías de preparaciones de polen con tinción de acetocarmin. El experimento se realizó en condiciones de invernadero y el diseño experimental fue de subparcelas divididas con 3 repeticiones; adicionalmente, los factores evaluados fueron: condiciones de humedad del suelo, inoculación con cepas de Bradyrhizobium sp. o Rhizobium tropici, y las líneas o accesiones evaluadas (6 mutantes y 2 no mutantes). El experimento fue realizado manteniendo la temperatura nocturna superior a 25°C. No hubo diferencias significativas entre los tratamientos, se encontraron correlaciones positivas de la viabilidad de polen con otras variables.application/pdfspaUniversidad Nacional de Colombia - Sede Palmirahttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/57704Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaCrespo-Muñoz, Sergio and Rivera-Peña, Mariela and Rosero-Alpala, Deisy Alexandra and Muñoz-Florez, Jaime Eduardo and Rao, Idupulapati M. and Muñoz-Florez, Ligia Carmenza (2018) Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia. Acta Agronómica, 67 (2). pp. 319-325. ISSN 2323-011855 Ciencias de la tierra / Earth sciences and geology63 Agricultura y tecnologías relacionadas / AgricultureAbiotic stresshigh temperaturehumidity contentFabaceaeRhizobiaceaeinoculationmutation induction.Altas temperaturasestrés abióticocontenido de humedadFabaceaeRhizobiaceaeinoculacióninducción de mutaciones.Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL57704-367292-6-PB.pdfapplication/pdf4437761https://repositorio.unal.edu.co/bitstream/unal/68090/1/57704-367292-6-PB.pdfdac93a678eb967964e5965b19fb0932eMD51THUMBNAIL57704-367292-6-PB.pdf.jpg57704-367292-6-PB.pdf.jpgGenerated Thumbnailimage/jpeg8356https://repositorio.unal.edu.co/bitstream/unal/68090/2/57704-367292-6-PB.pdf.jpg5e6321ffe815cbd8c2c20fa3328e5422MD52unal/68090oai:repositorio.unal.edu.co:unal/680902023-06-02 23:03:03.01Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |