Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia

Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morp...

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Autores:
Crespo-Muñoz, Sergio
Rivera-Peña, Mariela
Rosero-Alpala, Deisy Alexandra
Muñoz-Florez, Jaime Eduardo
Rao, Idupulapati M.
Muñoz-Florez, Ligia Carmenza
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/68090
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/68090
http://bdigital.unal.edu.co/69123/
Palabra clave:
55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Abiotic stress
high temperature
humidity content
Fabaceae
Rhizobiaceae
inoculation
mutation induction.
Altas temperaturas
estrés abiótico
contenido de humedad
Fabaceae
Rhizobiaceae
inoculación
inducción de mutaciones.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_ac68e316f428c29b9dd91feea0e0d137
oai_identifier_str oai:repositorio.unal.edu.co:unal/68090
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
title Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
spellingShingle Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Abiotic stress
high temperature
humidity content
Fabaceae
Rhizobiaceae
inoculation
mutation induction.
Altas temperaturas
estrés abiótico
contenido de humedad
Fabaceae
Rhizobiaceae
inoculación
inducción de mutaciones.
title_short Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
title_full Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
title_fullStr Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
title_full_unstemmed Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
title_sort Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia
dc.creator.fl_str_mv Crespo-Muñoz, Sergio
Rivera-Peña, Mariela
Rosero-Alpala, Deisy Alexandra
Muñoz-Florez, Jaime Eduardo
Rao, Idupulapati M.
Muñoz-Florez, Ligia Carmenza
dc.contributor.author.spa.fl_str_mv Crespo-Muñoz, Sergio
Rivera-Peña, Mariela
Rosero-Alpala, Deisy Alexandra
Muñoz-Florez, Jaime Eduardo
Rao, Idupulapati M.
Muñoz-Florez, Ligia Carmenza
dc.subject.ddc.spa.fl_str_mv 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
topic 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Abiotic stress
high temperature
humidity content
Fabaceae
Rhizobiaceae
inoculation
mutation induction.
Altas temperaturas
estrés abiótico
contenido de humedad
Fabaceae
Rhizobiaceae
inoculación
inducción de mutaciones.
dc.subject.proposal.spa.fl_str_mv Abiotic stress
high temperature
humidity content
Fabaceae
Rhizobiaceae
inoculation
mutation induction.
Altas temperaturas
estrés abiótico
contenido de humedad
Fabaceae
Rhizobiaceae
inoculación
inducción de mutaciones.
description Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morpho-physiological parameters on pollen viability and quantity in 123 mutant lines M5 of P. acutifolius and parental genotypes, by processing micrographs of pollen stained with acetocarmine. The experiment was conducted under greenhouse conditions and the experimental design was a split-split plot with three replicates; in addition, the factors evaluated were as follows: soil moisture conditions, inoculation with Bradyrhizobium sp. or Rhizobium tropici, and the evaluated lines (6 mutants and 2 non-mutants). The experiment was carried out maintaining night temperature above 25°C. There were no significant differences among treatments, and there was a positive correlation of pollen viability with other variables.
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018-04-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T05:49:15Z
dc.date.available.spa.fl_str_mv 2019-07-03T05:49:15Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2323-0118
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/68090
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/69123/
identifier_str_mv ISSN: 2323-0118
url https://repositorio.unal.edu.co/handle/unal/68090
http://bdigital.unal.edu.co/69123/
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dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/57704
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
dc.relation.references.spa.fl_str_mv Crespo-Muñoz, Sergio and Rivera-Peña, Mariela and Rosero-Alpala, Deisy Alexandra and Muñoz-Florez, Jaime Eduardo and Rao, Idupulapati M. and Muñoz-Florez, Ligia Carmenza (2018) Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia. Acta Agronómica, 67 (2). pp. 319-325. ISSN 2323-0118
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Palmira
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/68090/1/57704-367292-6-PB.pdf
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Crespo-Muñoz, Sergio531e3d75-f05c-4a63-908d-863f9f4b6333300Rivera-Peña, Marielac39e935a-9b75-4e5a-9f71-17aaa522f6f6300Rosero-Alpala, Deisy Alexandrafe23c423-18b5-48e0-b6da-63afd18b144c300Muñoz-Florez, Jaime Eduardode00a3e9-9ff7-4c8e-bcae-d5699ea4c1d4300Rao, Idupulapati M.ea5cd341-c7de-4996-8fe7-ebbd5dd68f3a300Muñoz-Florez, Ligia Carmenza4eaf8368-1994-410d-9963-2a13efea205c3002019-07-03T05:49:15Z2019-07-03T05:49:15Z2018-04-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/68090http://bdigital.unal.edu.co/69123/Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morpho-physiological parameters on pollen viability and quantity in 123 mutant lines M5 of P. acutifolius and parental genotypes, by processing micrographs of pollen stained with acetocarmine. The experiment was conducted under greenhouse conditions and the experimental design was a split-split plot with three replicates; in addition, the factors evaluated were as follows: soil moisture conditions, inoculation with Bradyrhizobium sp. or Rhizobium tropici, and the evaluated lines (6 mutants and 2 non-mutants). The experiment was carried out maintaining night temperature above 25°C. There were no significant differences among treatments, and there was a positive correlation of pollen viability with other variables.El fríjol tépari, Phaseolus acutifolius A. Gray destaca por su tolerancia al estrés por sequía y altas temperaturas, sin embargo, presenta baja diversidad genética en sus formas cultivadas. Por lo que se inició un programa de inducción de mutaciones. El objetivo de este estudio fue establecer el efecto de diferentes parámetros  agro-morfofisiológicos asociados sobre la viabilidad y cantidad del polen en 123 líneas mutantes de la quinta generación M5 de P. acutifolius y genotipos parentales, por medio del procesamiento de microfotografías de preparaciones de polen con tinción de acetocarmin. El experimento se realizó en condiciones de invernadero y el diseño experimental fue de subparcelas divididas con 3 repeticiones; adicionalmente, los factores evaluados fueron: condiciones de humedad del suelo, inoculación con cepas de Bradyrhizobium sp. o Rhizobium tropici, y las líneas o accesiones evaluadas (6 mutantes y 2 no mutantes). El experimento fue realizado manteniendo la temperatura nocturna superior a 25°C. No hubo diferencias significativas entre los tratamientos, se encontraron correlaciones positivas de la viabilidad de polen con otras variables.application/pdfspaUniversidad Nacional de Colombia - Sede Palmirahttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/57704Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaCrespo-Muñoz, Sergio and Rivera-Peña, Mariela and Rosero-Alpala, Deisy Alexandra and Muñoz-Florez, Jaime Eduardo and Rao, Idupulapati M. and Muñoz-Florez, Ligia Carmenza (2018) Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia. Acta Agronómica, 67 (2). pp. 319-325. ISSN 2323-011855 Ciencias de la tierra / Earth sciences and geology63 Agricultura y tecnologías relacionadas / AgricultureAbiotic stresshigh temperaturehumidity contentFabaceaeRhizobiaceaeinoculationmutation induction.Altas temperaturasestrés abióticocontenido de humedadFabaceaeRhizobiaceaeinoculacióninducción de mutaciones.Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL57704-367292-6-PB.pdfapplication/pdf4437761https://repositorio.unal.edu.co/bitstream/unal/68090/1/57704-367292-6-PB.pdfdac93a678eb967964e5965b19fb0932eMD51THUMBNAIL57704-367292-6-PB.pdf.jpg57704-367292-6-PB.pdf.jpgGenerated Thumbnailimage/jpeg8356https://repositorio.unal.edu.co/bitstream/unal/68090/2/57704-367292-6-PB.pdf.jpg5e6321ffe815cbd8c2c20fa3328e5422MD52unal/68090oai:repositorio.unal.edu.co:unal/680902023-06-02 23:03:03.01Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co