Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia

Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Penol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the...

Full description

Autores:
Gutiérrez, Pablo A.
Marín-Montoya, Mauricio
Muñoz-Baena, Laura
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61395
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61395
http://bdigital.unal.edu.co/60205/
Palabra clave:
63 Agricultura y tecnologías relacionadas / Agriculture
Genomics
molecular biology
Solanaceae
virus diseases.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_aaac1d33a7ec1a9f4690023a1136d165
oai_identifier_str oai:repositorio.unal.edu.co:unal/61395
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gutiérrez, Pablo A.5a088df3-e18a-4254-b2dd-4508b9543ab6300Marín-Montoya, Mauricio936d15fc-c0c5-45c6-b61c-b1c548ee7e80300Muñoz-Baena, Lauraac97b6ee-f197-4bf8-b8d5-aa71663dd6233002019-07-02T20:41:55Z2019-07-02T20:41:55Z2017-01-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/61395http://bdigital.unal.edu.co/60205/Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Penol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the chili and bell pepper transcriptomes resulted in consensus sequences of 14,727 nt and 14,714 nt that were identified as Bell pepper endornavirus (BPEV). Both sequences were nearly identical by 99.9 % at both nucleotide and amino acid levels. The presence of BPEV was confirmed by RT-qPCR, RT-PCR and Sanger sequencing using RdRp-specific primers designed from the assembled sequences in ten independent random samples taken from the investigated bell pepper stands. The phylogenetic analysis of both BPEV variants and their affiliation within the genus Endornavirus is discussed. For our knowledge, this is the first study on this group of viruses in Colombia.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/agrocol/article/view/60626Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaGutiérrez, Pablo A. and Marín-Montoya, Mauricio and Muñoz-Baena, Laura (2017) Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia. Agronomía Colombiana, 35 (1). pp. 44-53. ISSN 2357-373263 Agricultura y tecnologías relacionadas / AgricultureGenomicsmolecular biologySolanaceaevirus diseases.Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in ColombiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL60626-334768-1-PB.pdfapplication/pdf252896https://repositorio.unal.edu.co/bitstream/unal/61395/1/60626-334768-1-PB.pdf15f76d9f503d8d646986300fbb7cec45MD51THUMBNAIL60626-334768-1-PB.pdf.jpg60626-334768-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7727https://repositorio.unal.edu.co/bitstream/unal/61395/2/60626-334768-1-PB.pdf.jpgaa36877691c2a77d11a25e7c4ca74287MD52unal/61395oai:repositorio.unal.edu.co:unal/613952024-04-18 23:51:05.754Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
spellingShingle Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
63 Agricultura y tecnologías relacionadas / Agriculture
Genomics
molecular biology
Solanaceae
virus diseases.
title_short Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_full Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_fullStr Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_full_unstemmed Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_sort Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
dc.creator.fl_str_mv Gutiérrez, Pablo A.
Marín-Montoya, Mauricio
Muñoz-Baena, Laura
dc.contributor.author.spa.fl_str_mv Gutiérrez, Pablo A.
Marín-Montoya, Mauricio
Muñoz-Baena, Laura
dc.subject.ddc.spa.fl_str_mv 63 Agricultura y tecnologías relacionadas / Agriculture
topic 63 Agricultura y tecnologías relacionadas / Agriculture
Genomics
molecular biology
Solanaceae
virus diseases.
dc.subject.proposal.spa.fl_str_mv Genomics
molecular biology
Solanaceae
virus diseases.
description Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Penol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the chili and bell pepper transcriptomes resulted in consensus sequences of 14,727 nt and 14,714 nt that were identified as Bell pepper endornavirus (BPEV). Both sequences were nearly identical by 99.9 % at both nucleotide and amino acid levels. The presence of BPEV was confirmed by RT-qPCR, RT-PCR and Sanger sequencing using RdRp-specific primers designed from the assembled sequences in ten independent random samples taken from the investigated bell pepper stands. The phylogenetic analysis of both BPEV variants and their affiliation within the genus Endornavirus is discussed. For our knowledge, this is the first study on this group of viruses in Colombia.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017-01-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T20:41:55Z
dc.date.available.spa.fl_str_mv 2019-07-02T20:41:55Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.issn.spa.fl_str_mv ISSN: 2357-3732
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/61395
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/60205/
identifier_str_mv ISSN: 2357-3732
url https://repositorio.unal.edu.co/handle/unal/61395
http://bdigital.unal.edu.co/60205/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/agrocol/article/view/60626
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.references.spa.fl_str_mv Gutiérrez, Pablo A. and Marín-Montoya, Mauricio and Muñoz-Baena, Laura (2017) Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia. Agronomía Colombiana, 35 (1). pp. 44-53. ISSN 2357-3732
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/61395/1/60626-334768-1-PB.pdf
https://repositorio.unal.edu.co/bitstream/unal/61395/2/60626-334768-1-PB.pdf.jpg
bitstream.checksum.fl_str_mv 15f76d9f503d8d646986300fbb7cec45
aa36877691c2a77d11a25e7c4ca74287
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1814090254093647872

Publicaciones similares