Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia

Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Penol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the...

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Autores:
Gutiérrez, Pablo A.
Marín-Montoya, Mauricio
Muñoz-Baena, Laura
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61395
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61395
http://bdigital.unal.edu.co/60205/
Palabra clave:
63 Agricultura y tecnologías relacionadas / Agriculture
Genomics
molecular biology
Solanaceae
virus diseases.
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openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gutiérrez, Pablo A.5a088df3-e18a-4254-b2dd-4508b9543ab6300Marín-Montoya, Mauricio936d15fc-c0c5-45c6-b61c-b1c548ee7e80300Muñoz-Baena, Lauraac97b6ee-f197-4bf8-b8d5-aa71663dd6233002019-07-02T20:41:55Z2019-07-02T20:41:55Z2017-01-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/61395http://bdigital.unal.edu.co/60205/Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Penol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the chili and bell pepper transcriptomes resulted in consensus sequences of 14,727 nt and 14,714 nt that were identified as Bell pepper endornavirus (BPEV). Both sequences were nearly identical by 99.9 % at both nucleotide and amino acid levels. The presence of BPEV was confirmed by RT-qPCR, RT-PCR and Sanger sequencing using RdRp-specific primers designed from the assembled sequences in ten independent random samples taken from the investigated bell pepper stands. The phylogenetic analysis of both BPEV variants and their affiliation within the genus Endornavirus is discussed. For our knowledge, this is the first study on this group of viruses in Colombia.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/agrocol/article/view/60626Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaGutiérrez, Pablo A. and Marín-Montoya, Mauricio and Muñoz-Baena, Laura (2017) Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia. Agronomía Colombiana, 35 (1). pp. 44-53. ISSN 2357-373263 Agricultura y tecnologías relacionadas / AgricultureGenomicsmolecular biologySolanaceaevirus diseases.Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in ColombiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL60626-334768-1-PB.pdfapplication/pdf252896https://repositorio.unal.edu.co/bitstream/unal/61395/1/60626-334768-1-PB.pdf15f76d9f503d8d646986300fbb7cec45MD51THUMBNAIL60626-334768-1-PB.pdf.jpg60626-334768-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7727https://repositorio.unal.edu.co/bitstream/unal/61395/2/60626-334768-1-PB.pdf.jpgaa36877691c2a77d11a25e7c4ca74287MD52unal/61395oai:repositorio.unal.edu.co:unal/613952024-04-18 23:51:05.754Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
spellingShingle Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
63 Agricultura y tecnologías relacionadas / Agriculture
Genomics
molecular biology
Solanaceae
virus diseases.
title_short Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_full Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_fullStr Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_full_unstemmed Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
title_sort Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia
dc.creator.fl_str_mv Gutiérrez, Pablo A.
Marín-Montoya, Mauricio
Muñoz-Baena, Laura
dc.contributor.author.spa.fl_str_mv Gutiérrez, Pablo A.
Marín-Montoya, Mauricio
Muñoz-Baena, Laura
dc.subject.ddc.spa.fl_str_mv 63 Agricultura y tecnologías relacionadas / Agriculture
topic 63 Agricultura y tecnologías relacionadas / Agriculture
Genomics
molecular biology
Solanaceae
virus diseases.
dc.subject.proposal.spa.fl_str_mv Genomics
molecular biology
Solanaceae
virus diseases.
description Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Penol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the chili and bell pepper transcriptomes resulted in consensus sequences of 14,727 nt and 14,714 nt that were identified as Bell pepper endornavirus (BPEV). Both sequences were nearly identical by 99.9 % at both nucleotide and amino acid levels. The presence of BPEV was confirmed by RT-qPCR, RT-PCR and Sanger sequencing using RdRp-specific primers designed from the assembled sequences in ten independent random samples taken from the investigated bell pepper stands. The phylogenetic analysis of both BPEV variants and their affiliation within the genus Endornavirus is discussed. For our knowledge, this is the first study on this group of viruses in Colombia.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017-01-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T20:41:55Z
dc.date.available.spa.fl_str_mv 2019-07-02T20:41:55Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2357-3732
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/61395
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identifier_str_mv ISSN: 2357-3732
url https://repositorio.unal.edu.co/handle/unal/61395
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/agrocol/article/view/60626
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.references.spa.fl_str_mv Gutiérrez, Pablo A. and Marín-Montoya, Mauricio and Muñoz-Baena, Laura (2017) Genome sequencing of two iBell pepper endornavirus/i (BPEV) variants infecting iCapsicum annuum/i in Colombia. Agronomía Colombiana, 35 (1). pp. 44-53. ISSN 2357-3732
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
institution Universidad Nacional de Colombia
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