Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source

La microscopia holográfica digital sin lentes (DLHM) es tal vez la tecnología más simple en términos de hardware para la observación de muestras sin tinción; una fuente de iluminación coherente de ondas esféricas, un sistema digital de registro y un cuerpo mecánico para integrarlos, constituyen el h...

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Autores:
Tobon-Maya, Heberley
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86006
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86006
https://repositorio.unal.edu.co/
Palabra clave:
530 - Física
Procesamiento óptico de daos
Microscopia holográfica digital
Open-source Hardware
Open-Science
Digital holographic microscopy
Ciencia abierta
Microscopía holográfica digital
Microscopia óptica
Rights
openAccess
License
Atribución-NoComercial-CompartirIgual 4.0 Internacional
id UNACIONAL2_a79a27e6cb9882c3eeec5f91b99c10e7
oai_identifier_str oai:repositorio.unal.edu.co:unal/86006
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
dc.title.translated.eng.fl_str_mv Development of digital lensless holographic microscope prototype certifiable as open-source
title Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
spellingShingle Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
530 - Física
Procesamiento óptico de daos
Microscopia holográfica digital
Open-source Hardware
Open-Science
Digital holographic microscopy
Ciencia abierta
Microscopía holográfica digital
Microscopia óptica
title_short Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
title_full Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
title_fullStr Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
title_full_unstemmed Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
title_sort Desarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-source
dc.creator.fl_str_mv Tobon-Maya, Heberley
dc.contributor.advisor.none.fl_str_mv García Sucerquia, Jorge Iván
dc.contributor.author.none.fl_str_mv Tobon-Maya, Heberley
dc.contributor.researchgroup.spa.fl_str_mv Optica y Procesamiento Opto-Digital
dc.contributor.orcid.spa.fl_str_mv Tobón-Maya, Heberley [:0000-0002-3258-1861]
dc.contributor.researchgate.spa.fl_str_mv https://www.researchgate.net/profile/Heberley-Tobon-Maya
dc.contributor.googlescholar.spa.fl_str_mv https://scholar.google.com/citations?user=_JdH3vMAAAAJ&hl=es
dc.subject.ddc.spa.fl_str_mv 530 - Física
topic 530 - Física
Procesamiento óptico de daos
Microscopia holográfica digital
Open-source Hardware
Open-Science
Digital holographic microscopy
Ciencia abierta
Microscopía holográfica digital
Microscopia óptica
dc.subject.lemb.none.fl_str_mv Procesamiento óptico de daos
dc.subject.proposal.spa.fl_str_mv Microscopia holográfica digital
dc.subject.proposal.eng.fl_str_mv Open-source Hardware
Open-Science
Digital holographic microscopy
dc.subject.wikidata.none.fl_str_mv Ciencia abierta
Microscopía holográfica digital
Microscopia óptica
description La microscopia holográfica digital sin lentes (DLHM) es tal vez la tecnología más simple en términos de hardware para la observación de muestras sin tinción; una fuente de iluminación coherente de ondas esféricas, un sistema digital de registro y un cuerpo mecánico para integrarlos, constituyen el hardware necesario para su implementación, lo cual perfila a DLHM como una tecnología con un alto potencial de ser desarrollada de forma abierta, portable y accesible a un bajo costo. A pesar de este potencial las soluciones comerciales de DLHM se encuentran lejos de ser una tecnología accesible a diversos sectores de la educación, investigación y producción debido alto costo y baja portabilidad. En esta tesis de maestría en Ingeniería Física se condensan, refinan y articulan los diseños de DLHM producidos por el grupo de Óptica y Procesamiento Opto-Digital (GODP) relativos a los elementos constituyentes de la tecnología, para desarrollar un prototipo de microscopio DLHM certificable como hardware open-source. Para lograrlo se realizó un levantamiento del estado del arte de las características necesarias para que un prototipo de DLHM sea considerado como hardware open-source, se estudiaron los diferentes desarrollos del GDOP respecto a la fuente de iluminación y sistemas de registros en DLHM y finalmente se desarrolló un prototipo con base en los elementos constituyentes de mejor desempeño y las características necesarias su certificación. Los resultados de esta tesis se encuentran condensados en 2 artículos publicados en revistas indexadas, en la participación en 4 congresos de carácter internacional (LAOP 2022, RIAO 2023, ENO 2021, ETOP 2023) con 7 contribuciones en forma de presentación y un workshop de holografía digital dictado en el marco del 10th International Symposium "Optics & its applications” patrocinado por el ICTP. 8Texto tomado de la fuente)
publishDate 2023
dc.date.issued.none.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-05-02T14:35:19Z
dc.date.available.none.fl_str_mv 2024-05-02T14:35:19Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86006
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/86006
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.references.spa.fl_str_mv H. Tobon-Maya, S. Zapata-Valencia, E. Zora-Guzmán, C. Buitrago-Duque, and J. Garcia-Sucerquia, "Open-source, cost-effective, portable, 3D-printed digital lensless holographic microscope," Appl. Opt. 60, A205 (2021).
H. Tobon, C. Trujillo, and J. Garcia-Sucerquia, "Preprocessing in digital lensless holographic microscopy for intensity reconstructions with enhanced contrast," Appl. Opt. 60, A215 (2021).
H. Tobón-Maya, A. Gómez-Ramírez, C. Buitrago-Duque, and J. Garcia-Sucerquia, "Adapting a Blu-ray optical pickup unit as a point source for digital lensless holographic microscopy," Appl. Opt. 62, D39 (2023).
S. I. Zapata-Valencia, H. Tobon-Maya, and J. Garcia-Sucerquia, "Image enhancement and field of view enlargement in digital lensless holographic microscopy by multi-shot imaging," J. Opt. Soc. Am. A 40, C150 (2023).
H. Tobón-Maya, A. Gómez-Ramírez, and J. Garcia-Sucerquia, "Digital Lensless holographic microscopy based on an optical pick-up unit," in Latin America Optics and Photonics (LAOP) Conference 2022 (Optica Publishing Group, 2022), p. W1D.3.
S. I. Zapata-Valencia, H. Tobon-Maya, and J. García-Sucerquia, "Automatic method to measure the numerical aperture of a propagating Gaussian light beam," Opt. Pura y Apl. 55, 1–8 (2022).
C. A. Buitrago-Duque, S. I. Zapata-Valencia, H. Tobon-Maya, A. Gomez-Ramirez, and J. Garcia-Sucerquia, "Introduction to holography at undergraduate level using research-grade open-source software," in Seventeenth Conference on Education and Training in Optics and Photonics: ETOP 2023, M. McKee and D. J. Hagan, eds. (SPIE, 2023), Vol. 12723, p. 127231V. 122. S. I. Zapata-Valencia, A. Gómez-Ramírez, H.
S. I. Zapata-Valencia, A. Gómez-Ramírez, H. Tobon-Maya, C. A. Buitrago-Duque, and J. Garcia-Sucerquia, "Beyond maxima and minima: a hands-on approach for undergraduate teaching of diffraction," in Seventeenth Conference on Education and Training in Optics and Photonics: ETOP 2023, M. McKee and D. J. Hagan, eds. (SPIE, 2023), Vol. 12723, p. 1272302.
S. Amann, M. von Witzleben, Breuer, and Stefan, "3D-printable portable opensource platform for low-cost lens-less holographic cellular imaging," arXiv Prepr. arXiv1904.04497 (2019)
B. Patiño-Jurado, J. F. Botero-Cadavid, and J. Garcia-Sucerquia, "Step-Index Optical Fibers With 0.88 Numerical Aperture," J. Light. Technol. 37, 3734–3739 (2019).
B. Patiño-Jurado, J. F. Botero-Cadavid, and J. Garcia-Sucerquia, "Digital Lensless Holographic Microscopy with Engineered Optical Fiber Point Source," in Digital Holography and Three-Dimensional Imaging 2019 (OSA, 2019), p. Th3A.5.
P. Piedrahita-Quintero, C. Trujillo, and J. Garcia-Sucerquia, "JDiffraction: A GPGPU-accelerated JAVA library for numerical propagation of scalar wave fields," Comput. Phys. Commun. 214, 128–139 (2017).
R. Antoniou, J. Bonvoisin, P.-Y. Hsing, E. Dekoninck, and D. Defazio, "Defining success in open source hardware development projects: a survey of practitioners," Des. Sci. 8, e8 (2022).
A. Powell, "Democratizing production through open source knowledge: from open software to open hardware," Media, Cult. Soc. 34, 691–708 (2012).
N. Chakravorty, C. S. Sharma, K. A. Molla, and J. K. Pattanaik, "Open Science: Challenges, Possible Solutions and the Way Forward," Proc. Indian Natl. Sci. Acad. 88, 456–471 (2022).
M. P. da COSTA and F. C. L. LEITE, "Open access in the world and Latin America: A review since the Budapest Open Access Initiative," Transinformação 28, 33–46 (2016).
E. Commission, D.-G. for Research, Innovation, C. Cabello Valdes, B. Rentier, E. Kaunismaa, J. Metcalfe, F. Esposito, D. McAllister, K. Maas, K. Vandevelde, and C. O’Carroll, Evaluation of Research Careers Fully Acknowledging Open Science Practices : Rewards, Incentives and/or Recognition for Researchers Practicing Open Science (Publications Office, 2017).
J. M. Pearce, "Economic savings for scientific free and open source technology: A review," HardwareX 8, e00139 (2020)
G. Niezen, P. Eslambolchilar, and H. Thimbleby, "Open-source hardware for medical devices," BMJ Innov. 2, 78–83 (2016).
M. Vázquez, L. Anfossi, H. Ben-Yoav, L. Diéguez, T. Karopka, B. Della Ventura, S. Abalde-Cela, A. Minopoli, F. Di Nardo, V. K. Shukla, A. Teixeira, A. Tvarijonaviciute, and L. Franco-Martínez, "Use of some cost-effective technologies for a routine clinical pathology laboratory," Lab Chip 21, 4330–4351 (2021)
J. T. Collins, J. Knapper, J. Stirling, J. Mduda, C. Mkindi, V. Mayagaya, G. A. Mwakajinga, P. T. Nyakyi, V. L. Sanga, D. Carbery, L. White, S. Dale, Z. Jieh Lim, J. J. Baumberg, P. Cicuta, S. McDermott, B. Vodenicharski, and R. Bowman, "Robotic microscopy for everyone: the OpenFlexure microscope," Biomed. Opt. Express 11, 2447 (2020)
G. Popescu, Quantitative Phase Imaging of Cells and Tissues (McGraw Hill Professional, 2011).
A. Saetchnikov, V. Saetchnikov, E. Tcherniavskaia, and A. Ostendorf, "Effect of a thin reflective film between substrate and photoresin on two-photon polymerization," Addit. Manuf. 24, 658–666 (2018).
L. Xu, X. Peng, J. Miao, and A. K. Asundi, "Studies of digital microscopic holography with applications to microstructure testing," Appl. Opt. 40, 5046 (2001).
J. Garcia-Sucerquia, W. Xu, S. K. Jericho, P. Klages, M. H. Jericho, and H. J. Kreuzer, "Digital in-line holographic microscopy," Appl. Opt. 45, 836 (2006).
J. W. Goodman, Introduction to Fourier Optics, 3rd ed. (Roberts & Company Publishers, 2005).
J. Garcia-Sucerquia, W. Xu, S. K. K. Jericho, M. H. H. Jericho, P. Klages, and H. J. J. Kreuzer, "Resolution power in digital in-line holography," in Proceedings of SPIE - The International Society for Optical Engineering (2006), Vol. 6027 I.
E. J. Davis, M. Jones, D. A. Thiel, and S. Pauls, "Using Open-Source, 3D Printable Optical Hardware To Enhance Student Learning in the Instrumental Analysis Laboratory," J. Chem. Educ. 95, 672–677 (2018)
M. Sanz, J. Á. Picazo-Bueno, L. Granero, J. García, and V. Micó, "Multi-illumination single-holographic-exposure lensless Fresnel (MISHELF) microscopy using 4 channels," in Imaging and Applied Optics 2019 (COSI, IS, MATH, PcAOP), OSA Technical Digest (Optical Society of America, 2019), p. JW2A.1
Hamilton Company, "Needle Gauge Chart," https://www.hamiltoncompany.com/laboratory-products/needles-knowledge/needlegauge-chart
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dc.format.extent.spa.fl_str_mv 1 recurso en línea (91 páginas)
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Medellín - Ciencias - Maestría en Ingeniería Física
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Medellín
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2García Sucerquia, Jorge Iván437dafe36f928d676c13766f53a05980600Tobon-Maya, Heberley9a78152b0241577457e0f6eb403576e7600Optica y Procesamiento Opto-DigitalTobón-Maya, Heberley [:0000-0002-3258-1861]https://www.researchgate.net/profile/Heberley-Tobon-Mayahttps://scholar.google.com/citations?user=_JdH3vMAAAAJ&hl=es2024-05-02T14:35:19Z2024-05-02T14:35:19Z2023https://repositorio.unal.edu.co/handle/unal/86006Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/La microscopia holográfica digital sin lentes (DLHM) es tal vez la tecnología más simple en términos de hardware para la observación de muestras sin tinción; una fuente de iluminación coherente de ondas esféricas, un sistema digital de registro y un cuerpo mecánico para integrarlos, constituyen el hardware necesario para su implementación, lo cual perfila a DLHM como una tecnología con un alto potencial de ser desarrollada de forma abierta, portable y accesible a un bajo costo. A pesar de este potencial las soluciones comerciales de DLHM se encuentran lejos de ser una tecnología accesible a diversos sectores de la educación, investigación y producción debido alto costo y baja portabilidad. En esta tesis de maestría en Ingeniería Física se condensan, refinan y articulan los diseños de DLHM producidos por el grupo de Óptica y Procesamiento Opto-Digital (GODP) relativos a los elementos constituyentes de la tecnología, para desarrollar un prototipo de microscopio DLHM certificable como hardware open-source. Para lograrlo se realizó un levantamiento del estado del arte de las características necesarias para que un prototipo de DLHM sea considerado como hardware open-source, se estudiaron los diferentes desarrollos del GDOP respecto a la fuente de iluminación y sistemas de registros en DLHM y finalmente se desarrolló un prototipo con base en los elementos constituyentes de mejor desempeño y las características necesarias su certificación. Los resultados de esta tesis se encuentran condensados en 2 artículos publicados en revistas indexadas, en la participación en 4 congresos de carácter internacional (LAOP 2022, RIAO 2023, ENO 2021, ETOP 2023) con 7 contribuciones en forma de presentación y un workshop de holografía digital dictado en el marco del 10th International Symposium "Optics & its applications” patrocinado por el ICTP. 8Texto tomado de la fuente)Digital Lensless Holographic Microscopy (DLHM) is perhaps the simplest technology in terms of hardware for label-free sample observation. A coherent spherical wave illumination source, a digital recording system, and a mechanical body for integration, are the necessary hardware components for its implementation. This simplicity of hardaware sets DLHM as a technology with high potential for open, portable, and cost-effective development. Despite this potential, commercial DLHM solutions are far from being accessible to many sectors in education, research, and production, mainly due to its high costs and low portability. In this Master's thesis in Engineering in Physics, the designs of DLHM developed by the Optics and Opto-Digital Processing group (GODP) concerning the constituent elements of the technology are condensed, refined, and articulated in the development of a certifiable open-source hardware prototype of a DLHM microscope. To achieve this, a survey of the state-of-the-art characteristics necessary for a DLHM prototype to be considered as opensource hardware was conducted. The different developments of GOPD regarding the illumination source and recording systems in DLHM were studied, and finally, a prototype was developed based on the best-performing constituent elements and the necessary certification characteristics. The results of this thesis are condensed in two articles published in indexed journals and the participation in four international congresses (LAOP 2022, RIAO 2023, ENO 2021, ETOP 2023) with seven contributions in the form of presentations, and a workshop on digital holography delivered within the framework of the 10th International Symposium "Optics & its applications," sponsored by the ICTPMaestríaMaestría en Ciencias - FísicaÓptica AplicadaÁrea Curricular en Física1 recurso en línea (91 páginas)application/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ingeniería FísicaFacultad de CienciasUniversidad Nacional de Colombia - Sede Medellín530 - FísicaProcesamiento óptico de daosMicroscopia holográfica digitalOpen-source HardwareOpen-ScienceDigital holographic microscopyCiencia abiertaMicroscopía holográfica digitalMicroscopia ópticaDesarrollo de un prototipo de microscopio holográfico digital sin lentes certificable open-sourceDevelopment of digital lensless holographic microscope prototype certifiable as open-sourceTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMH. Tobon-Maya, S. Zapata-Valencia, E. Zora-Guzmán, C. Buitrago-Duque, and J. Garcia-Sucerquia, "Open-source, cost-effective, portable, 3D-printed digital lensless holographic microscope," Appl. Opt. 60, A205 (2021).H. Tobon, C. Trujillo, and J. Garcia-Sucerquia, "Preprocessing in digital lensless holographic microscopy for intensity reconstructions with enhanced contrast," Appl. Opt. 60, A215 (2021).H. Tobón-Maya, A. Gómez-Ramírez, C. Buitrago-Duque, and J. Garcia-Sucerquia, "Adapting a Blu-ray optical pickup unit as a point source for digital lensless holographic microscopy," Appl. Opt. 62, D39 (2023).S. I. Zapata-Valencia, H. Tobon-Maya, and J. Garcia-Sucerquia, "Image enhancement and field of view enlargement in digital lensless holographic microscopy by multi-shot imaging," J. Opt. Soc. Am. A 40, C150 (2023).H. Tobón-Maya, A. Gómez-Ramírez, and J. Garcia-Sucerquia, "Digital Lensless holographic microscopy based on an optical pick-up unit," in Latin America Optics and Photonics (LAOP) Conference 2022 (Optica Publishing Group, 2022), p. W1D.3.S. I. Zapata-Valencia, H. Tobon-Maya, and J. García-Sucerquia, "Automatic method to measure the numerical aperture of a propagating Gaussian light beam," Opt. Pura y Apl. 55, 1–8 (2022).C. A. Buitrago-Duque, S. I. Zapata-Valencia, H. Tobon-Maya, A. Gomez-Ramirez, and J. Garcia-Sucerquia, "Introduction to holography at undergraduate level using research-grade open-source software," in Seventeenth Conference on Education and Training in Optics and Photonics: ETOP 2023, M. McKee and D. J. Hagan, eds. (SPIE, 2023), Vol. 12723, p. 127231V. 122. S. I. Zapata-Valencia, A. Gómez-Ramírez, H.S. I. Zapata-Valencia, A. Gómez-Ramírez, H. Tobon-Maya, C. A. Buitrago-Duque, and J. Garcia-Sucerquia, "Beyond maxima and minima: a hands-on approach for undergraduate teaching of diffraction," in Seventeenth Conference on Education and Training in Optics and Photonics: ETOP 2023, M. McKee and D. J. Hagan, eds. (SPIE, 2023), Vol. 12723, p. 1272302.S. Amann, M. von Witzleben, Breuer, and Stefan, "3D-printable portable opensource platform for low-cost lens-less holographic cellular imaging," arXiv Prepr. arXiv1904.04497 (2019)B. Patiño-Jurado, J. F. Botero-Cadavid, and J. Garcia-Sucerquia, "Step-Index Optical Fibers With 0.88 Numerical Aperture," J. Light. Technol. 37, 3734–3739 (2019).B. Patiño-Jurado, J. F. Botero-Cadavid, and J. Garcia-Sucerquia, "Digital Lensless Holographic Microscopy with Engineered Optical Fiber Point Source," in Digital Holography and Three-Dimensional Imaging 2019 (OSA, 2019), p. Th3A.5.P. Piedrahita-Quintero, C. Trujillo, and J. Garcia-Sucerquia, "JDiffraction: A GPGPU-accelerated JAVA library for numerical propagation of scalar wave fields," Comput. Phys. Commun. 214, 128–139 (2017).R. Antoniou, J. Bonvoisin, P.-Y. Hsing, E. Dekoninck, and D. Defazio, "Defining success in open source hardware development projects: a survey of practitioners," Des. Sci. 8, e8 (2022).A. Powell, "Democratizing production through open source knowledge: from open software to open hardware," Media, Cult. Soc. 34, 691–708 (2012).N. Chakravorty, C. S. Sharma, K. A. Molla, and J. K. Pattanaik, "Open Science: Challenges, Possible Solutions and the Way Forward," Proc. Indian Natl. Sci. Acad. 88, 456–471 (2022).M. P. da COSTA and F. C. L. LEITE, "Open access in the world and Latin America: A review since the Budapest Open Access Initiative," Transinformação 28, 33–46 (2016).E. Commission, D.-G. for Research, Innovation, C. Cabello Valdes, B. Rentier, E. Kaunismaa, J. Metcalfe, F. Esposito, D. McAllister, K. Maas, K. Vandevelde, and C. O’Carroll, Evaluation of Research Careers Fully Acknowledging Open Science Practices : Rewards, Incentives and/or Recognition for Researchers Practicing Open Science (Publications Office, 2017).J. M. Pearce, "Economic savings for scientific free and open source technology: A review," HardwareX 8, e00139 (2020)G. Niezen, P. Eslambolchilar, and H. Thimbleby, "Open-source hardware for medical devices," BMJ Innov. 2, 78–83 (2016).M. Vázquez, L. Anfossi, H. Ben-Yoav, L. Diéguez, T. Karopka, B. Della Ventura, S. Abalde-Cela, A. Minopoli, F. Di Nardo, V. K. Shukla, A. Teixeira, A. Tvarijonaviciute, and L. Franco-Martínez, "Use of some cost-effective technologies for a routine clinical pathology laboratory," Lab Chip 21, 4330–4351 (2021)J. T. Collins, J. Knapper, J. Stirling, J. Mduda, C. Mkindi, V. Mayagaya, G. A. Mwakajinga, P. T. Nyakyi, V. L. Sanga, D. Carbery, L. White, S. Dale, Z. Jieh Lim, J. J. Baumberg, P. Cicuta, S. McDermott, B. Vodenicharski, and R. 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