Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión

ilustraciones, fotografías, gráficas, tablas

Autores:
Gómez Guerrero, Néstor Alejandro
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/85327
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/85327
https://repositorio.unal.edu.co/
Palabra clave:
540 - Química y ciencias afines::543 - Química analítica
Doping en los Deportes
Péptidos
Doping in Sports
Peptides
Péptidos cortos
Cromatografía líquida
Espectrometría de masas
Control al dopaje
matriz biológica
Short peptides
Liquid chromatography
Mass spectrometry
Doping control
Biological matrix
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_a64fe4a3434a19213ec266d050d1adbb
oai_identifier_str oai:repositorio.unal.edu.co:unal/85327
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
dc.title.translated.eng.fl_str_mv Liquid Chromatography Mass Spectrometry (LC-MS) as a tool for the analysis of small peptides of interest in doping: A review
title Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
spellingShingle Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
540 - Química y ciencias afines::543 - Química analítica
Doping en los Deportes
Péptidos
Doping in Sports
Peptides
Péptidos cortos
Cromatografía líquida
Espectrometría de masas
Control al dopaje
matriz biológica
Short peptides
Liquid chromatography
Mass spectrometry
Doping control
Biological matrix
title_short Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
title_full Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
title_fullStr Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
title_full_unstemmed Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
title_sort Cromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: Revisión
dc.creator.fl_str_mv Gómez Guerrero, Néstor Alejandro
dc.contributor.advisor.none.fl_str_mv Rivera Monroy, Zuly Jenny
dc.contributor.author.none.fl_str_mv Gómez Guerrero, Néstor Alejandro
dc.contributor.researchgroup.spa.fl_str_mv Síntesis y Aplicación de Moléculas Peptídicas
dc.subject.ddc.spa.fl_str_mv 540 - Química y ciencias afines::543 - Química analítica
topic 540 - Química y ciencias afines::543 - Química analítica
Doping en los Deportes
Péptidos
Doping in Sports
Peptides
Péptidos cortos
Cromatografía líquida
Espectrometría de masas
Control al dopaje
matriz biológica
Short peptides
Liquid chromatography
Mass spectrometry
Doping control
Biological matrix
dc.subject.decs.spa.fl_str_mv Doping en los Deportes
Péptidos
dc.subject.decs.eng.fl_str_mv Doping in Sports
Peptides
dc.subject.proposal.spa.fl_str_mv Péptidos cortos
Cromatografía líquida
Espectrometría de masas
Control al dopaje
matriz biológica
dc.subject.proposal.eng.fl_str_mv Short peptides
Liquid chromatography
Mass spectrometry
Doping control
Biological matrix
description ilustraciones, fotografías, gráficas, tablas
publishDate 2023
dc.date.issued.none.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-16T15:35:12Z
dc.date.available.none.fl_str_mv 2024-01-16T15:35:12Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/85327
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/85327
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados al autor, 2023http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rivera Monroy, Zuly Jennybcc909fcc85ff0efa659b7aaca42b3cbGómez Guerrero, Néstor Alejandrofdf04c307f41be92489d8ebe079716b4Síntesis y Aplicación de Moléculas Peptídicas2024-01-16T15:35:12Z2024-01-16T15:35:12Z2023https://repositorio.unal.edu.co/handle/unal/85327Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasLos péptidos cortos se consideran, en el ámbito del control al dopaje, como un grupo separado de sustancias dopantes de acuerdo con la clasificación de la Agencia Mundial Antidopaje (AMA). Se trata de un grupo diverso, que incluye, entre otros, la hormona de crecimiento, los factores de liberación de la hormona del crecimiento y los análogos de la hormona liberadora de gonadotropina. En la última década se han logrado importantes desarrollos en la detección de péptidos cortos de uso frecuente en dopaje, por tal motivo, se revisaron los principales avances en la extracción, limpieza y preparación de muestras biológicas para la detección de péptidos cortos mediante cromatografía líquida y espectrometría de masas (LC-MS) como insumo para el desarrollo y validación de metodologías analíticas que permitan la detección rutinaria de péptidos cortos en laboratorios de control al dopaje. De igual manera, se revisó la síntesis de péptidos cortos como alternativa para la fabricación de materiales de referencia necesarios para el análisis de péptidos cortos usados en dopaje. Por otra parte, se revisaron las modificaciones más frecuentes de los péptidos cortos, su uso en el mercado negro y su metabolismo, como fuente de información necesaria para descubrir nuevos marcadores que permitan ampliar la ventana de detección de los péptidos cortos usados en dopaje. (Texto tomado de la fuente)Short peptides are considered, in the field of doping control, as a separate group of doping substances according to the classification of the World Anti-Doping Agency (WADA). This is a diverse group, including, but not limited to, growth hormone, growth hormone releasing factors, and gonadotropin-releasing hormone analogues. In the last decade, important developments have been achieved in the detection of short peptides frequently used in doping, for this reason, the main advances in the extraction, cleaning and preparation of biological samples for the detection of short peptides by liquid chromatography and mass spectrometry (LC-MS) were reviewed as an input for the development and validation of analytical methodologies that allow the routine detection of short peptides in doping control laboratories. Similarly, the synthesis of short peptides was reviewed as an alternative for the manufacture of reference materials necessary for the analysis of short peptides used in doping. On the other hand, the most frequent modifications of short peptides, their use in the black market and their metabolism were reviewed, as a source of information necessary to discover new markers that allow expanding the detection window of short peptides used in doping.MaestríaMagíster en Ciencias - QuímicaQuímica analíticaxx, 70 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::543 - Química analíticaDoping en los DeportesPéptidosDoping in SportsPeptidesPéptidos cortosCromatografía líquidaEspectrometría de masasControl al dopajematriz biológicaShort peptidesLiquid chromatographyMass spectrometryDoping controlBiological matrixCromatografía Líquida acoplada a Espectrometría de Masas (LC-MS) como herramienta de análisis de péptidos cortos de interés en el dopaje: RevisiónLiquid Chromatography Mass Spectrometry (LC-MS) as a tool for the analysis of small peptides of interest in doping: A reviewTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TM1. 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WwgdHJhdGFtaWVudG8gZGUgZGF0b3MgcGVyc29uYWxlcywgZGUgYWN1ZXJkbyBjb24gbGEgbGV5IDE1ODEgZGUgMjAxMiBlbnRlbmRpZW5kbyBxdWUgc2UgZW5jdWVudHJhbiBiYWpvIG1lZGlkYXMgcXVlIGdhcmFudGl6YW4gbGEgc2VndXJpZGFkLCBjb25maWRlbmNpYWxpZGFkIGUgaW50ZWdyaWRhZCwgeSBzdSB0cmF0YW1pZW50byB0aWVuZSB1bmEgZmluYWxpZGFkIGhpc3TDs3JpY2EsIGVzdGFkw61zdGljYSBvIGNpZW50w61maWNhIHNlZ8O6biBsbyBkaXNwdWVzdG8gZW4gbGEgUG9sw610aWNhIGRlIFRyYXRhbWllbnRvIGRlIERhdG9zIFBlcnNvbmFsZXMuCgoKClBBUlRFIDIuIEFVVE9SSVpBQ0nDk04gUEFSQSBQVUJMSUNBUiBZIFBFUk1JVElSIExBIENPTlNVTFRBIFkgVVNPIERFIE9CUkFTIEVOIEVMIFJFUE9TSVRPUklPIElOU1RJVFVDSU9OQUwgVU5BTC4KClNlIGF1dG9yaXphIGxhIHB1YmxpY2FjacOzbiBlbGVjdHLDs25pY2EsIGNvbnN1bHRhIHkgdXNvIGRlIGxhIG9icmEgcG9yIHBhcnRlIGRlIGxhIFVuaXZlcnNpZGFkIE5hY2lvbmFsIGRlIENvbG9tYmlhIHkgZGUgc3VzIHVzdWFyaW9zIGRlIGxhIHNpZ3VpZW50ZSBtYW5lcmE6CgphLglDb25jZWRvIGxpY2VuY2lhIGVuIGxvcyB0w6lybWlub3Mgc2XDsWFsYWRvcyBlbiBsYSBwYXJ0ZSAxIGRlbCBwcmVzZW50ZSBkb2N1bWVudG8sIGNvbiBlbCBvYmpldGl2byBkZSBxdWUgbGEgb2JyYSBlbnRyZWdhZGEgc2VhIHB1YmxpY2FkYSBlbiBlbCBSZXBvc2l0b3JpbyBJbnN0aXR1Y2lvbmFsIGRlIGxhIFVuaXZlcnNpZGFkIE5hY2lvbmFsIGRlIENvbG9tYmlhIHkgcHVlc3RhIGEgZGlzcG9zaWNpw7NuIGVuIGFjY2VzbyBhYmllcnRvIHBhcmEgc3UgY29uc3VsdGEgcG9yIGxvcyB1c3VhcmlvcyBkZSBsYSBVbml2ZXJzaWRhZCBOYWNpb25hbCBkZSBDb2xvbWJpYSAgYSB0cmF2w6lzIGRlIGludGVybmV0LgoKCgpQQVJURSAzIEFVVE9SSVpBQ0nDk04gREUgVFJBVEFNSUVOVE8gREUgREFUT1MgUEVSU09OQUxFUy4KCkxhIFVuaXZlcnNpZGFkIE5hY2lvbmFsIGRlIENvbG9tYmlhLCBjb21vIHJlc3BvbnNhYmxlIGRlbCBUcmF0YW1pZW50byBkZSBEYXRvcyBQZXJzb25hbGVzLCBpbmZvcm1hIHF1ZSBsb3MgZGF0b3MgZGUgY2Fyw6FjdGVyIHBlcnNvbmFsIHJlY29sZWN0YWRvcyBtZWRpYW50ZSBlc3RlIGZvcm11bGFyaW8sIHNlIGVuY3VlbnRyYW4gYmFqbyBtZWRpZGFzIHF1ZSBnYXJhbnRpemFuIGxhIHNlZ3VyaWRhZCwgY29uZmlkZW5jaWFsaWRhZCBlIGludGVncmlkYWQgeSBzdSB0cmF0YW1pZW50byBzZSByZWFsaXphIGRlIGFjdWVyZG8gYWwgY3VtcGxpbWllbnRvIG5vcm1hdGl2byBkZSBsYSBMZXkgMTU4MSBkZSAyMDEyIHkgZGUgbGEgUG9sw610aWNhIGRlIFRyYXRhbWllbnRvIGRlIERhdG9zIFBlcnNvbmFsZXMgZGUgbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgZGUgQ29sb21iaWEuIFB1ZWRlIGVqZXJjZXIgc3VzIGRlcmVjaG9zIGNvbW8gdGl0dWxhciBhIGNvbm9jZXIsIGFjdHVhbGl6YXIsIHJlY3RpZmljYXIgeSByZXZvY2FyIGxhcyBhdXRvcml6YWNpb25lcyBkYWRhcyBhIGxhcyBmaW5hbGlkYWRlcyBhcGxpY2FibGVzIGEgdHJhdsOpcyBkZSBsb3MgY2FuYWxlcyBkaXNwdWVzdG9zIHkgZGlzcG9uaWJsZXMgZW4gd3d3LnVuYWwuZWR1LmNvIG8gZS1tYWlsOiBwcm90ZWNkYXRvc19uYUB1bmFsLmVkdS5jbyIKClRlbmllbmRvIGVuIGN1ZW50YSBsbyBhbnRlcmlvciwgYXV0b3Jpem8gZGUgbWFuZXJhIHZvbHVudGFyaWEsIHByZXZpYSwgZXhwbMOtY2l0YSwgaW5mb3JtYWRhIGUgaW5lcXXDrXZvY2EgYSBsYSBVbml2ZXJzaWRhZCBOYWNpb25hbCBkZSBDb2xvbWJpYSBhIHRyYXRhciBsb3MgZGF0b3MgcGVyc29uYWxlcyBkZSBhY3VlcmRvIGNvbiBsYXMgZmluYWxpZGFkZXMgZXNwZWPDrWZpY2FzIHBhcmEgZWwgZGVzYXJyb2xsbyB5IGVqZXJjaWNpbyBkZSBsYXMgZnVuY2lvbmVzIG1pc2lvbmFsZXMgZGUgZG9jZW5jaWEsIGludmVzdGlnYWNpw7NuIHkgZXh0ZW5zacOzbiwgYXPDrSBjb21vIGxhcyByZWxhY2lvbmVzIGFjYWTDqW1pY2FzLCBsYWJvcmFsZXMsIGNvbnRyYWN0dWFsZXMgeSB0b2RhcyBsYXMgZGVtw6FzIHJlbGFjaW9uYWRhcyBjb24gZWwgb2JqZXRvIHNvY2lhbCBkZSBsYSBVbml2ZXJzaWRhZC4gCgo=

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