Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns
Aromatic and medicinal plant species are a valuable resource for research and development of pharmaceutical, cosmetic, crop protection and nutritional agents, due to the high amount of bioactive phytochemicals that they contain. However, these compounds are a major obstacle in the isolation of high-...
- Autores:
-
Vega Vela, Nelson Enrique
Chacón Sánchez, María Isabel
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2011
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/35867
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/35867
http://bdigital.unal.edu.co/25948/
- Palabra clave:
- 63 Agricultura y tecnologías relacionadas / Agriculture
chaotropic agent
guanidinium cation
silica-based extraction columns
nucleic acid extraction kit
guanidinium salts
molecular markers.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| title |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| spellingShingle |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns 63 Agricultura y tecnologías relacionadas / Agriculture chaotropic agent guanidinium cation silica-based extraction columns nucleic acid extraction kit guanidinium salts molecular markers. |
| title_short |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| title_full |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| title_fullStr |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| title_full_unstemmed |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| title_sort |
Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns |
| dc.creator.fl_str_mv |
Vega Vela, Nelson Enrique Chacón Sánchez, María Isabel |
| dc.contributor.author.spa.fl_str_mv |
Vega Vela, Nelson Enrique Chacón Sánchez, María Isabel |
| dc.subject.ddc.spa.fl_str_mv |
63 Agricultura y tecnologías relacionadas / Agriculture |
| topic |
63 Agricultura y tecnologías relacionadas / Agriculture chaotropic agent guanidinium cation silica-based extraction columns nucleic acid extraction kit guanidinium salts molecular markers. |
| dc.subject.proposal.spa.fl_str_mv |
chaotropic agent guanidinium cation silica-based extraction columns nucleic acid extraction kit guanidinium salts molecular markers. |
| description |
Aromatic and medicinal plant species are a valuable resource for research and development of pharmaceutical, cosmetic, crop protection and nutritional agents, due to the high amount of bioactive phytochemicals that they contain. However, these compounds are a major obstacle in the isolation of high-quality DNA suitable for genetic analyses. In this paper, we report a protocol that optimizes the use of the cationic detergent CTAB and the reductant β-mercaptoethanol in cell lysis. The elimination of plant secondary metabolites such as polysaccharides and polyphenols, that typically co-isolate with DNA, was achieved using the chemical denaturing properties of the guanidinium cation, which together with the adsorbent chemical specificity of the silica, resulted in the purification of high-quality DNA suitable for digestion with restriction enzymes and optimal for PCR amplification of AFLP-type molecular markers. This protocol was evaluated on 16 Colombian aromatic and medicinal plant species promising for their essential oils. The results allow suggesting that this procedure might be appropriate for other species, tissues and sample types recalcitrant to DNA extraction. |
| publishDate |
2011 |
| dc.date.issued.spa.fl_str_mv |
2011-09-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-28T00:43:38Z |
| dc.date.available.spa.fl_str_mv |
2019-06-28T00:43:38Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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ISSN: 2357-3732 |
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https://repositorio.unal.edu.co/handle/unal/35867 |
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http://bdigital.unal.edu.co/25948/ |
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ISSN: 2357-3732 |
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https://repositorio.unal.edu.co/handle/unal/35867 http://bdigital.unal.edu.co/25948/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/agrocol/article/view/23460 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
| dc.relation.references.spa.fl_str_mv |
Vega Vela, Nelson Enrique and Chacón Sánchez, María Isabel (2011) Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns. Agronomía Colombiana, 29 (3). pp. 349-357. ISSN 2357-3732 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial |
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vega Vela, Nelson Enriquea715a0e8-9aa3-45b8-b216-1ea7ebb63510300Chacón Sánchez, María Isabel2e75f198-e9b2-4992-a855-0cc6a2fb6ff73002019-06-28T00:43:38Z2019-06-28T00:43:38Z2011-09-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/35867http://bdigital.unal.edu.co/25948/Aromatic and medicinal plant species are a valuable resource for research and development of pharmaceutical, cosmetic, crop protection and nutritional agents, due to the high amount of bioactive phytochemicals that they contain. However, these compounds are a major obstacle in the isolation of high-quality DNA suitable for genetic analyses. In this paper, we report a protocol that optimizes the use of the cationic detergent CTAB and the reductant β-mercaptoethanol in cell lysis. The elimination of plant secondary metabolites such as polysaccharides and polyphenols, that typically co-isolate with DNA, was achieved using the chemical denaturing properties of the guanidinium cation, which together with the adsorbent chemical specificity of the silica, resulted in the purification of high-quality DNA suitable for digestion with restriction enzymes and optimal for PCR amplification of AFLP-type molecular markers. This protocol was evaluated on 16 Colombian aromatic and medicinal plant species promising for their essential oils. The results allow suggesting that this procedure might be appropriate for other species, tissues and sample types recalcitrant to DNA extraction.Las especies vegetales aromáticas y medicinales son un recurso valioso para la investigación y el desarrollo de agentes farmacéuticos, cosméticos, agrícolas y de alimentos, debido a la alta cantidad de fitoquímicos bioactivos que contienen. Sin embargo, estos compuestos son un obstáculo en el aislamiento de ADN de alta calidad adecuado para análisis genéticos. En el presente artículo reportamos un protocolo que optimiza el uso del detergente catiónico CTAB y el agente reductor β-mercaptoetanol en la lisis celular. La eliminación de metabolitos secundarios como polisacáridos y polifenoles que típicamente co-aíslan con el ADN, se logró utilizando las propiedades químicas desnaturalizantes del catión guanidinio que junto con la especificidad química adsorbente de la sílica, resultó en la purificación de ADN de alta calidad adecuado para la digestión por enzimas de restricción y óptimo para la amplificación por PCR de marcadores moleculares tipo AFLPs. Este protocolo se evaluó en 16 especies vegetales aromáticas y medicinales colombianas promisorias por sus aceites esenciales. Los resultados obtenidos permiten sugerir que este procedimiento podría ser apropiado para su aplicación en otras especies, tejidos y tipos de muestras recalcitrantes a la extracción de ADN.application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/23460Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaVega Vela, Nelson Enrique and Chacón Sánchez, María Isabel (2011) Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columns. Agronomía Colombiana, 29 (3). pp. 349-357. ISSN 2357-373263 Agricultura y tecnologías relacionadas / Agriculturechaotropic agentguanidinium cationsilica-based extraction columnsnucleic acid extraction kitguanidinium saltsmolecular markers.Isolation of high-quality dna in 16 aromatic and medicinal colombian species using silica-based extraction columnsArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL23460-115254-2-PB.pdfapplication/pdf833992https://repositorio.unal.edu.co/bitstream/unal/35867/1/23460-115254-2-PB.pdf079e608880352c6ffb3ebf9bad5a6b97MD51THUMBNAIL23460-115254-2-PB.pdf.jpg23460-115254-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8803https://repositorio.unal.edu.co/bitstream/unal/35867/2/23460-115254-2-PB.pdf.jpg5e938ab9e03a5205f3f799d99637b690MD52unal/35867oai:repositorio.unal.edu.co:unal/358672023-01-07 23:02:01.552Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |