Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco

Ilustraciones, tablas

Autores:
García Torres, Alexandra
Tipo de recurso:
Fecha de publicación:
2021
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
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oai:repositorio.unal.edu.co:unal/80078
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/80078
https://repositorio.unal.edu.co/
Palabra clave:
580 - Plantas
630 - Agricultura y tecnologías relacionadas
Begomovirus
Genómica
Inoculación de virus
Geminivirus
Tomate
Tabaco
Biobalística
Pseudorecombinación
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id UNACIONAL2_a198110868d005cfd7a3cef85a09deee
oai_identifier_str oai:repositorio.unal.edu.co:unal/80078
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
dc.title.translated.eng.fl_str_mv Preliminary study of the interaction of geminivirus taking tomato and tobacco plants as model
title Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
spellingShingle Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
580 - Plantas
630 - Agricultura y tecnologías relacionadas
Begomovirus
Genómica
Inoculación de virus
Geminivirus
Tomate
Tabaco
Biobalística
Pseudorecombinación
title_short Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
title_full Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
title_fullStr Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
title_full_unstemmed Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
title_sort Estudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabaco
dc.creator.fl_str_mv García Torres, Alexandra
dc.contributor.advisor.none.fl_str_mv Vaca Vaca, Juan Carlos
López López, Karina
dc.contributor.author.none.fl_str_mv García Torres, Alexandra
dc.contributor.researchgroup.spa.fl_str_mv IPMA Interacción Planta -Microorganismo -Ambiente
dc.subject.ddc.spa.fl_str_mv 580 - Plantas
630 - Agricultura y tecnologías relacionadas
topic 580 - Plantas
630 - Agricultura y tecnologías relacionadas
Begomovirus
Genómica
Inoculación de virus
Geminivirus
Tomate
Tabaco
Biobalística
Pseudorecombinación
dc.subject.agrovoc.none.fl_str_mv Begomovirus
Genómica
Inoculación de virus
dc.subject.proposal.spa.fl_str_mv Geminivirus
Tomate
Tabaco
Biobalística
Pseudorecombinación
description Ilustraciones, tablas
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-09-02T15:00:21Z
dc.date.available.none.fl_str_mv 2021-09-02T15:00:21Z
dc.date.issued.none.fl_str_mv 2021-08
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/80078
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/80078
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial-SinDerivadas 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombia, 2021http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vaca Vaca, Juan Carlos00eb815dddd9b4cdb89d771e732014eaLópez López, Karina8d1d6de07e4436ad2c444998e0e02c80García Torres, Alexandra6194e7f4c3d37687375104fcb84de62cIPMA Interacción Planta -Microorganismo -Ambiente2021-09-02T15:00:21Z2021-09-02T15:00:21Z2021-08https://repositorio.unal.edu.co/handle/unal/80078Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, tablasNumerosas enfermedades causadas por begomovirus han sido el resultado de eventos de pseudorecombinación, el cual involucra procesos de transcomplementación y transreplicación. El virus del mosaico amarillo de la papa (PYMV) es un begomovirus que afecta el cultivo del tomate en Colombia, el virus del mosaico dorado de croton (CohGMV), el virus del mosaico dorado de rhynchosia de Colombia (RhGMCV), y el virus moteado de verbena (VeMV) fueron aislados de las arvenses Croton hirtus, Rhynchosia minima y Verbena sp. respectivamente, todas ellas asociadas al cultivo de tomate en Valle del Cauca. El objetivo de este trabajo fue evaluar la posibilidad de que se presenten eventos de transcomplementación heteróloga entre los componentes genómicos A y B de los begomovirus aislados de estas arvenses y PYMV. Para cumplir este objetivo, se co- bombardearon por biobalistica hojas jóvenes de tomate y tabaco con mezclas de componentes genómicos de PYMV-A, PYMV-B, CohGMV-A, RhGMCV-A, y VeMV-B. Posibles interacciones virus-virus entre PYMV, CohGMV, RhGMCV, y VeMV, fueron estudiadas en tres niveles a los 21 y 45 dpi: replicación, movimiento y expresión de síntomas. La replicación y movimiento fue evaluada por la detección de los virus inoculados en hojas bombardeadas y hojas apicales jóvenes, mediante PCR empleando oligos específicos para cada virus estudiado. Finalmente, análisis bioinformaticos de los promotores de los genes AL1, AR1, BL1 y BR1 de PYMV, CohGMV, RhGMCV, y VeMV fueron realizados para identificar elementos cis regulatorios comprometidos en replicación (interones) y movimiento (CLEs). Se evidenciaron eventos de transreplicación y transcomplementación en todas las combinaciones heterólogas evaluadas en plantas de tomate, y en algunas evaluadas en tabaco. Este resultado podría indicar que a futuro podrían emerger nuevos pseudorecombinantes begomovirales a partir de la combinación de los componentes genómicos de PYMV, CohGMV, RhGMCV y VeMV, con probabilidad de mayor capacidad infectiva para el cultivo de tomate en Colombia (Texto tomado de la fuente).MaestríaMagíster en Ciencias AgrariasSe germinaron semillas de tabaco (Nicotiana tabacum var. xanthi) in vitro. Las semillas fueron desinfestadas con hipoclorito de sodio al 2%, etanol al 70% y agua destilada estéril, todo este proceso se llevó a cabo en la cabina de flujo laminar, ya que el proceso de germinación se realizó in vitro en medio basal MS (Murashige and Skoog, 1962). Se plantó una semilla por frasco, se llevaron al cuarto de crecimiento con condiciones controladas con un fotoperiodo 16 – 8 h de luz. Este proceso se llevó a cabo en el laboratorio de Cultivo de Téjidos Vegetales. Transcurridos 15 días de germinación y con cuatro hojas verdaderas las plántulas de tomate variedad Santa Clara fueron trasplantadas a materas con capacidad de 1 Kilo que previamente habían sido llenadas con suelo estéril, las plántulas se protegieron en jaulas con malla antitrips ubicándolas en la casa de malla de la Universidad Nacional de Colombia sede Palmira. Una vez pasados 7 días de trasplante, se realizó una fertilización con 15-15-15 complejo N-P-K CENAGRO ® de manera líquida, para ello se utilizaron 30 gr de fertilizante en un litro de agua dejando en agitación media por 24 horas, una vez diluido completamente el fertilizante se adiciono a cada planta a 20ml de la solución; esta fue aplicada hacia el plato de planta con el fin de evitar cualquier daño por quema. Esta fertilización se hizo con base a el manejo convencional que se hace en campo en este cultivo, así se evitaba posibles falsos positivos cuando se presentaran los síntomasProtección de Cultivos113 páginasapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ciencias Agropecuarias - Maestría en Ciencias AgrariasFacultad de Ciencias AgrariasUniversidad Nacional de Colombia - Sede Palmira580 - Plantas630 - Agricultura y tecnologías relacionadasBegomovirusGenómicaInoculación de virusGeminivirusTomateTabacoBiobalísticaPseudorecombinaciónEstudio preliminar de la interacción de geminivirus tomando como modelo plantas de tomate y tabacoPreliminary study of the interaction of geminivirus taking tomato and tobacco plants as modelTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMAccotto, G. P., Mullineaux, P. M., Brown, S. C., and Marie, D. (1993). 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Journal of virology, 85(22), 11821-11832.“Evaluación de la capacidad de infectar cultivos de tomate (solanum lycopersicum L.) y tabaco (nicotiana tabacum L.) de begomovirus aislados de arvenses localizadas en el Valle del Cauca, Colombia”.DIPALLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/80078/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL52954110.2021.pdf52954110.2021.pdfTesis de maestria en ciencias agrariasapplication/pdf1180590https://repositorio.unal.edu.co/bitstream/unal/80078/3/52954110.2021.pdf479724190ff7a742f3ee2891fdeb6b36MD53THUMBNAIL52954110.2021.pdf.jpg52954110.2021.pdf.jpgGenerated Thumbnailimage/jpeg4957https://repositorio.unal.edu.co/bitstream/unal/80078/4/52954110.2021.pdf.jpg2af76f50019a157d88581e41e3a540bdMD54unal/80078oai:repositorio.unal.edu.co:unal/800782023-07-23 23:03:57.557Repositorio Institucional Universidad Nacional de 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