Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were...
- Autores:
-
Pinilla, Laura
Toro, León F.
Avignone-Rossa, Claudio
Peñuela, Mariana
Rios-Estepa, Rigoberto
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/68526
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/68526
http://bdigital.unal.edu.co/69559/
- Palabra clave:
- 62 Ingeniería y operaciones afines / Engineering
ácido clavulánico
diseño de medios de cultivo
selección de cepa
Streptomyces clavuligerus
antibióticos
clavulanic acid
culture media design
strain selection
Streptomyces clavuligerus
antibiotics
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| id |
UNACIONAL2_9fa72d01aff37cac46f7d15a65c71b8a |
|---|---|
| oai_identifier_str |
oai:repositorio.unal.edu.co:unal/68526 |
| network_acronym_str |
UNACIONAL2 |
| network_name_str |
Universidad Nacional de Colombia |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| title |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| spellingShingle |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis 62 Ingeniería y operaciones afines / Engineering ácido clavulánico diseño de medios de cultivo selección de cepa Streptomyces clavuligerus antibióticos clavulanic acid culture media design strain selection Streptomyces clavuligerus antibiotics |
| title_short |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| title_full |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| title_fullStr |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| title_full_unstemmed |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| title_sort |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis |
| dc.creator.fl_str_mv |
Pinilla, Laura Toro, León F. Avignone-Rossa, Claudio Peñuela, Mariana Rios-Estepa, Rigoberto |
| dc.contributor.author.spa.fl_str_mv |
Pinilla, Laura Toro, León F. Avignone-Rossa, Claudio Peñuela, Mariana Rios-Estepa, Rigoberto |
| dc.subject.ddc.spa.fl_str_mv |
62 Ingeniería y operaciones afines / Engineering |
| topic |
62 Ingeniería y operaciones afines / Engineering ácido clavulánico diseño de medios de cultivo selección de cepa Streptomyces clavuligerus antibióticos clavulanic acid culture media design strain selection Streptomyces clavuligerus antibiotics |
| dc.subject.proposal.spa.fl_str_mv |
ácido clavulánico diseño de medios de cultivo selección de cepa Streptomyces clavuligerus antibióticos clavulanic acid culture media design strain selection Streptomyces clavuligerus antibiotics |
| description |
The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production. |
| publishDate |
2018 |
| dc.date.issued.spa.fl_str_mv |
2018-04-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-03T07:01:59Z |
| dc.date.available.spa.fl_str_mv |
2019-07-03T07:01:59Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.issn.spa.fl_str_mv |
ISSN: 2346-2183 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/68526 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/69559/ |
| identifier_str_mv |
ISSN: 2346-2183 |
| url |
https://repositorio.unal.edu.co/handle/unal/68526 http://bdigital.unal.edu.co/69559/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/dyna/article/view/69560 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Dyna Dyna |
| dc.relation.references.spa.fl_str_mv |
Pinilla, Laura and Toro, León F. and Avignone-Rossa, Claudio and Peñuela, Mariana and Rios-Estepa, Rigoberto (2018) Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis. DYNA, 85 (205). pp. 111-118. ISSN 2346-2183 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
| dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia - Sede Medellín - Facultad de Minas |
| institution |
Universidad Nacional de Colombia |
| bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/68526/1/69560-384329-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/68526/2/69560-384329-1-PB.pdf.jpg |
| bitstream.checksum.fl_str_mv |
3f21bb2dcdb2ac8bae3c5146b84a928b b8a56e93e5d930d4afbbe4a290794bc5 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
| _version_ |
1806886078063312896 |
| spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pinilla, Laurae12f1c1d-10be-44a0-8666-9b8531d2ee15300Toro, León F.c9083c05-a7bb-4a0a-b02b-99fe507cd0a2300Avignone-Rossa, Claudio99ede6ae-1ffa-4824-802e-0890111e8788300Peñuela, Mariana84f8d0c5-d819-487b-957f-6a76d3082b0e300Rios-Estepa, Rigoberto8949061e-2f21-4945-b4f1-e3abd0b1835f3002019-07-03T07:01:59Z2019-07-03T07:01:59Z2018-04-01ISSN: 2346-2183https://repositorio.unal.edu.co/handle/unal/68526http://bdigital.unal.edu.co/69559/The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production.La importancia clínica e industrial del ácido clavulánico (AC) promueve la búsqueda de nuevas estrategias para aumentar su productividad a un menor costo. En esta contribución, se evaluó la producción de AC en dos cepas de referencia de Streptomyces clavuligerus (Sc), ATCC27064 y DSM41826; éstas se probaron en diferentes medios de cultivo; los rendimientos de producto y costo de los medios se usaron como criterios de selección. En promedio, ScATCC alcanzó una concentración de AC 1.6-veces mayor que ScDSM, en el medio de proteína de soya (ISP). Se optimizó el medio con un diseño experimental central compuesto, estudiando la influencia del ISP y K2HPO4 sobre la producción de AC. La concentración máxima de AC obtenida en el medio optimizado (56mg·L-1) estuvo cerca del óptimo teórico. Nuestros resultados confirman que medios de cultivo con fuentes de carbono como glicerol y/o almidón proporcionan las mejores condiciones para la producción de AC.application/pdfspaUniversidad Nacional de Colombia - Sede Medellín - Facultad de Minashttps://revistas.unal.edu.co/index.php/dyna/article/view/69560Universidad Nacional de Colombia Revistas electrónicas UN DynaDynaPinilla, Laura and Toro, León F. and Avignone-Rossa, Claudio and Peñuela, Mariana and Rios-Estepa, Rigoberto (2018) Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis. DYNA, 85 (205). pp. 111-118. ISSN 2346-218362 Ingeniería y operaciones afines / Engineeringácido clavulánicodiseño de medios de cultivoselección de cepaStreptomyces clavuligerusantibióticosclavulanic acidculture media designstrain selectionStreptomyces clavuligerusantibioticsStreptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysisArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL69560-384329-1-PB.pdfapplication/pdf607376https://repositorio.unal.edu.co/bitstream/unal/68526/1/69560-384329-1-PB.pdf3f21bb2dcdb2ac8bae3c5146b84a928bMD51THUMBNAIL69560-384329-1-PB.pdf.jpg69560-384329-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg10001https://repositorio.unal.edu.co/bitstream/unal/68526/2/69560-384329-1-PB.pdf.jpgb8a56e93e5d930d4afbbe4a290794bc5MD52unal/68526oai:repositorio.unal.edu.co:unal/685262023-06-04 23:03:10.236Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |