Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis

The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were...

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Autores:
Pinilla, Laura
Toro, León F.
Avignone-Rossa, Claudio
Peñuela, Mariana
Rios-Estepa, Rigoberto
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/68526
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/68526
http://bdigital.unal.edu.co/69559/
Palabra clave:
62 Ingeniería y operaciones afines / Engineering
ácido clavulánico
diseño de medios de cultivo
selección de cepa
Streptomyces clavuligerus
antibióticos
clavulanic acid
culture media design
strain selection
Streptomyces clavuligerus
antibiotics
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_9fa72d01aff37cac46f7d15a65c71b8a
oai_identifier_str oai:repositorio.unal.edu.co:unal/68526
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
title Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
spellingShingle Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
62 Ingeniería y operaciones afines / Engineering
ácido clavulánico
diseño de medios de cultivo
selección de cepa
Streptomyces clavuligerus
antibióticos
clavulanic acid
culture media design
strain selection
Streptomyces clavuligerus
antibiotics
title_short Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
title_full Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
title_fullStr Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
title_full_unstemmed Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
title_sort Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis
dc.creator.fl_str_mv Pinilla, Laura
Toro, León F.
Avignone-Rossa, Claudio
Peñuela, Mariana
Rios-Estepa, Rigoberto
dc.contributor.author.spa.fl_str_mv Pinilla, Laura
Toro, León F.
Avignone-Rossa, Claudio
Peñuela, Mariana
Rios-Estepa, Rigoberto
dc.subject.ddc.spa.fl_str_mv 62 Ingeniería y operaciones afines / Engineering
topic 62 Ingeniería y operaciones afines / Engineering
ácido clavulánico
diseño de medios de cultivo
selección de cepa
Streptomyces clavuligerus
antibióticos
clavulanic acid
culture media design
strain selection
Streptomyces clavuligerus
antibiotics
dc.subject.proposal.spa.fl_str_mv ácido clavulánico
diseño de medios de cultivo
selección de cepa
Streptomyces clavuligerus
antibióticos
clavulanic acid
culture media design
strain selection
Streptomyces clavuligerus
antibiotics
description The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production.
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018-04-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T07:01:59Z
dc.date.available.spa.fl_str_mv 2019-07-03T07:01:59Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2346-2183
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/68526
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url https://repositorio.unal.edu.co/handle/unal/68526
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dc.language.iso.spa.fl_str_mv spa
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dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/dyna/article/view/69560
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Dyna
Dyna
dc.relation.references.spa.fl_str_mv Pinilla, Laura and Toro, León F. and Avignone-Rossa, Claudio and Peñuela, Mariana and Rios-Estepa, Rigoberto (2018) Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis. DYNA, 85 (205). pp. 111-118. ISSN 2346-2183
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Medellín - Facultad de Minas
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pinilla, Laurae12f1c1d-10be-44a0-8666-9b8531d2ee15300Toro, León F.c9083c05-a7bb-4a0a-b02b-99fe507cd0a2300Avignone-Rossa, Claudio99ede6ae-1ffa-4824-802e-0890111e8788300Peñuela, Mariana84f8d0c5-d819-487b-957f-6a76d3082b0e300Rios-Estepa, Rigoberto8949061e-2f21-4945-b4f1-e3abd0b1835f3002019-07-03T07:01:59Z2019-07-03T07:01:59Z2018-04-01ISSN: 2346-2183https://repositorio.unal.edu.co/handle/unal/68526http://bdigital.unal.edu.co/69559/The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production.La importancia clínica e industrial del ácido clavulánico (AC) promueve la búsqueda de nuevas estrategias para aumentar su productividad a un menor costo. En esta contribución, se evaluó la producción de AC en dos cepas de referencia de Streptomyces clavuligerus (Sc), ATCC27064 y DSM41826; éstas se probaron en diferentes medios de cultivo; los rendimientos de producto y costo de los medios se usaron como criterios de selección. En promedio, ScATCC alcanzó una concentración de AC 1.6-veces mayor que ScDSM, en el medio de proteína de soya (ISP). Se optimizó el medio con un diseño experimental central compuesto, estudiando la influencia del ISP y K2HPO4 sobre la producción de AC. La concentración máxima de AC obtenida en el medio optimizado (56mg·L-1) estuvo cerca del óptimo teórico. Nuestros resultados confirman que medios de cultivo con fuentes de carbono como glicerol y/o almidón proporcionan las mejores condiciones para la producción de AC.application/pdfspaUniversidad Nacional de Colombia - Sede Medellín - Facultad de Minashttps://revistas.unal.edu.co/index.php/dyna/article/view/69560Universidad Nacional de Colombia Revistas electrónicas UN DynaDynaPinilla, Laura and Toro, León F. and Avignone-Rossa, Claudio and Peñuela, Mariana and Rios-Estepa, Rigoberto (2018) Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis. DYNA, 85 (205). pp. 111-118. ISSN 2346-218362 Ingeniería y operaciones afines / Engineeringácido clavulánicodiseño de medios de cultivoselección de cepaStreptomyces clavuligerusantibióticosclavulanic acidculture media designstrain selectionStreptomyces clavuligerusantibioticsStreptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysisArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL69560-384329-1-PB.pdfapplication/pdf607376https://repositorio.unal.edu.co/bitstream/unal/68526/1/69560-384329-1-PB.pdf3f21bb2dcdb2ac8bae3c5146b84a928bMD51THUMBNAIL69560-384329-1-PB.pdf.jpg69560-384329-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg10001https://repositorio.unal.edu.co/bitstream/unal/68526/2/69560-384329-1-PB.pdf.jpgb8a56e93e5d930d4afbbe4a290794bc5MD52unal/68526oai:repositorio.unal.edu.co:unal/685262023-06-04 23:03:10.236Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co