Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)

Ilustraciones, tablas

Autores:: Jaramillo Valencia, Angélica María

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
dc.title.translated.eng.fl_str_mv	Orange (OR) protein role in the production and accumulation of β-carotene in cassava roots (Manihot esculenta Crantz)
title	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
spellingShingle	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz) 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Carotenoides Mandioca cassava Biofortificación regulación postranscripcional PCR tiempo real cuantitativo Manihot esculenta Biofortification posttranscriptional regulation quantitative real time PCR
title_short	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
title_full	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
title_fullStr	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
title_full_unstemmed	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
title_sort	Función de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)
dc.creator.fl_str_mv	Jaramillo Valencia, Angélica María
dc.contributor.advisor.none.fl_str_mv	Ocampo, Jhon Albeiro Álvarez Álvarez, Daniel
dc.contributor.author.none.fl_str_mv	Jaramillo Valencia, Angélica María
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic	630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Carotenoides Mandioca cassava Biofortificación regulación postranscripcional PCR tiempo real cuantitativo Manihot esculenta Biofortification posttranscriptional regulation quantitative real time PCR
dc.subject.agrovoc.none.fl_str_mv	Carotenoides Mandioca cassava
dc.subject.proposal.spa.fl_str_mv	Biofortificación regulación postranscripcional PCR tiempo real cuantitativo
dc.subject.proposal.other.fl_str_mv	Manihot esculenta
dc.subject.proposal.eng.fl_str_mv	Biofortification posttranscriptional regulation quantitative real time PCR
description	Ilustraciones, tablas
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-07-28T15:19:47Z
dc.date.available.none.fl_str_mv	2021-07-28T15:19:47Z
dc.date.issued.none.fl_str_mv	2021
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.coarversion.spa.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	publishedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/79860
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/79860 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.spa.fl_str_mv	Derechos reservados - Universidad Nacional de Colombia, 2021
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dc.format.extent.spa.fl_str_mv	87 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Palmira - Ciencias Agropecuarias - Maestría en Ciencias Biológicas
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias Agropecuarias
dc.publisher.place.spa.fl_str_mv	Palmira Valle del Cauca
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Palmira
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombia, 2021http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ocampo, Jhon Albeiro631e706ac5a1235a653c7220186a0da3Álvarez Álvarez, Daniel834a5ca8532c8c48aaa04199948d9c4dJaramillo Valencia, Angélica Maríad67de92c8fe7f0f17b64668e2bd05fb72021-07-28T15:19:47Z2021-07-28T15:19:47Z2021https://repositorio.unal.edu.co/handle/unal/79860Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, tablasEl desarrollo de nuevos cultivares de yuca con alto contenido de carotenoides hace parte de una estrategia para combatir la deficiencia de vitamina A gracias a su amplio consumo a nivel mundial. Orange (OR) y fitoeno sintasa (PSY) son proteínas reguladoras en la producción de carotenoides, pero la función de OR, así como su relación con PSY, no ha sido estudiada en yuca. El objetivo de este trabajo fue estudiar la función de la proteína OR en la producción de β-caroteno en raíces de yuca y su relación con PSY. Para esto se realizó un análisis bioinformático para identificar genes codificantes de OR en yuca, y posteriormente se llevó a cabo un estudio de expresión de los genes detectados en raíces de un genotipo de yuca blanca (60444) y dos genotipos de yuca amarilla (GM5309-57 y GM3736-37). Los datos se analizaron mediante un análisis univariado de varianza y se utilizó una prueba de comparación de medias Dunnett (p˂0.05) con el programa SAS (v9.3). Los resultados mostraron la presencia de cuatro genes hipotéticos OR con porcentajes de identidad con Arabidopsis thaliana entre 65,8 y 76,3%. El nivel de transcritos de los genes OR permanecieron constantes, mientras que se encontró una mayor acumulación de proteína OR en los genotipos amarillos. Asimismo, se observó una sobreexpresión de 3,7 veces del gen PSY1 y una disminución de 4,7 y 1,6 veces para NCED y BCH, respectivamente, lo cual podría relacionarse con un posible mecanismo de atenuación del catabolismo del β-caroteno promovido por OR. Mientras que se encontraron diferencias significativas (p˂0.05) en los genotipos amarillos en comparación con el blanco para el contenido de carotenos, proteína OR y expresión génica de PSY1 y NCED, el gen BCH presentó diferencias significativas solo en uno de los genotipos amarillos. Los resultados obtenidos invitan a futuras investigaciones enfocadas al mejoramiento del contenido de carotenoides en la yuca.The development of new cassava cultivars with high concentration of carotenoids is part of a strategy to combat vitamin A deficiency due to its wide consumption worldwide. Orange (OR) and phytoene synthase (PSY) are regulatory proteins in the production of carotenoids, but OR function, as well as its relationship with PSY, has not yet been studied in cassava. The aim of this work has been the study if the OR protein function upon the production of β-carotene in cassava roots, as well as its relationship with PSY protein. A bioinformatic analysis was carried out to identify cassava OR genes, and a subsequent expression study of the detected genes in roots of one white (60444) and two yellow (GM5309-57 and GM3736-37) cassava genotypes was conducted. Data was analyzed with a univariate analysis of variance and a Dunnett mean comparison test (p˂ 0.05) with the SAS program (v9.3). Results showed the presence of four hypothetical OR genes with identity percentages with Arabidopsis thaliana between 65.8 and 76.3%. The transcripts levels of the OR genes remained constant, while a higher accumulation of OR protein was found in the yellow genotypes. Likewise, a 3.7-fold overexpression of PSY1 and a 4.7- and 1.6-fold decrease for NCED and BCH were observed, respectively, which could be related to a possible mechanism of attenuation of the catabolism of β-carotene promoted by OR. Whereas significant differences (p˂ 0.05) were found in yellow genotypes compared to white for carotene content, protein OR and gene expression of PSY1 and NCED. the BCH gene presented only significant differences in one of the yellow genotypes. These results encourage further research aiming to carotenoids enhancement in cassava.MaestríaMagíster en Ciencias BiológicasLa secuencia de la región de codificación (CDS) del gen OR de Arabidopsis thaliana (AtOR) (AY117226.1) se utilizó como referencia para la búsqueda de los genes hipotéticos de OR en el genoma de la yuca en Phytozome. Se seleccionaron tres genotipos de yuca provenientes del programa de mejoramiento de yuca del CIAT (3° 30’N, 76° 21’O; 965 msnm, Palmira, Colombia). El material seleccionado correspondió a un genotipo con raíz de color blanco (60444) y dos genotipos con pulpa amarilla (GM 5309-57 y GM3736-37).87 páginasapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ciencias Agropecuarias - Maestría en Ciencias BiológicasFacultad de Ciencias AgropecuariasPalmira Valle del CaucaUniversidad Nacional de Colombia - Sede Palmira630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesCarotenoidesMandiocacassavaBiofortificaciónregulación postranscripcionalPCR tiempo real cuantitativoManihot esculentaBiofortificationposttranscriptional regulationquantitative real time PCRFunción de la proteína Orange (OR) en la producción y acumulación de β-caroteno en raíces de yuca (Manihot esculenta Crantz)Orange (OR) protein role in the production and accumulation of β-carotene in cassava roots (Manihot esculenta Crantz)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMAhrazem, O., López, A. 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Proceedings of the National Academy of Sciences of the United States of America, 105(47), 18232–18237. https://doi.org/10.1073/pnas.0809737105ORIGINAL1130612219.2021.pdf1130612219.2021.pdfTesis de maestría en ciencias biológicasapplication/pdf3386223https://repositorio.unal.edu.co/bitstream/unal/79860/7/1130612219.2021.pdfc0fae70acd129be21a33dcb23bb9882cMD57LICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79860/4/license.txtcccfe52f796b7c63423298c2d3365fc6MD54CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://repositorio.unal.edu.co/bitstream/unal/79860/6/license_rdf4460e5956bc1d1639be9ae6146a50347MD56THUMBNAIL1130612219.2021.pdf.jpg1130612219.2021.pdf.jpgGenerated Thumbnailimage/jpeg5364https://repositorio.unal.edu.co/bitstream/unal/79860/8/1130612219.2021.pdf.jpg1f161988f5cb6403dba3a26f78820ad9MD58unal/79860oai:repositorio.unal.edu.co:unal/798602023-07-23 23:03:42.362Repositorio Institucional 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GVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCgpVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSAtIMOabHRpbWEgbW9kaWZpY2FjacOzbiAyNy8yMC8yMDIwCg==

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