Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation

In this work was carried out the development and quantification of an analytical methodology for quantification of Warfarin Sodium in an extemporaneous suspension for hospital use. Evaluation of specificity was achieved against its excipients and degradation compounds. The selectivity was evaluated...

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Autores:
Berrío, Marcel
Trujillo, Mary
Vallejo, Bibiana Margarita
Barbosa, Hélber de Jesús
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/49323
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/49323
http://bdigital.unal.edu.co/42780/
http://bdigital.unal.edu.co/42780/2/
Palabra clave:
Warfarina
validación
HPLC-DAD
preparación extemporánea
Warfarin
validation
HPLC-DAD
extemporaneous preparation
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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oai_identifier_str oai:repositorio.unal.edu.co:unal/49323
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Berrío, Marcel8ba8e33a-e212-4029-a9f5-b24bc989a252300Trujillo, Marycd7b4462-fd78-40fc-9652-da5b9b56eb88300Vallejo, Bibiana Margarita8c1d3d15-7bed-48ff-969a-5dec8ce9926d300Barbosa, Hélber de Jesús038f01ac-7c49-47a9-a83f-0f185af3f37d3002019-06-29T08:35:26Z2019-06-29T08:35:26Z2013https://repositorio.unal.edu.co/handle/unal/49323http://bdigital.unal.edu.co/42780/http://bdigital.unal.edu.co/42780/2/In this work was carried out the development and quantification of an analytical methodology for quantification of Warfarin Sodium in an extemporaneous suspension for hospital use. Evaluation of specificity was achieved against its excipients and degradation compounds. The selectivity was evaluated against the excipients and degradation compounds. Linearity studies were performed in a range of concentration between 0.05 to 0.15 mg/mL. Precision was evaluated in levels of repeatability and intermediate precision and accuracy in three concentration levels corresponding to the 75, 100 and 125%. The results show that the validation of the methodology is selective, linear, accurate and precise; therefore, it is reliable for use in the quantification of active warfarin sodium in the extemporaneous suspension.En este trabajo se llevó a cabo el desarrollo y la validación de una metodología analítica por HPLC para la cuantificación de Warfarina Sódica en una suspensión para uso hospitalario. La selectividad se evaluó frente a los excipientes y compuestos de degradación; la linealidad en el rango de concentraciones comprendido entre 0,05 y 0,15 mg/mL; la precisión se estudió en los niveles de repetibilidad y precisión intermedia y la exactitud en tres niveles de concentración que corresponden al 75%, 100% y 125%. Los resultados muestran que la validación de la metodología por HPLC es selectiva, lineal, precisa y exacta; por tanto, es confiable para ser utilizada en la cuantificación del activo Warfarina Sódica en una suspensión extemporánea y también en estudios de estabilidad de dicha preparación.application/pdfspaRevista Colombiana de Ciencias Químico Farmacéuticashttp://revistas.unal.edu.co/index.php/rccquifa/article/view/45106Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Ciencias Químico FarmacéuticasRevista Colombiana de Ciencias Químico FarmacéuticasRevista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 122-133 0034-7418 1909-6356Berrío, Marcel and Trujillo, Mary and Vallejo, Bibiana Margarita and Barbosa, Hélber de Jesús (2013) Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation. Revista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 122-133 0034-7418 1909-6356 .Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparationArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTWarfarinavalidaciónHPLC-DADpreparación extemporáneaWarfarinvalidationHPLC-DADextemporaneous preparationORIGINAL45106-216321-1-SM.pdfapplication/pdf440668https://repositorio.unal.edu.co/bitstream/unal/49323/1/45106-216321-1-SM.pdf33aa322beb935165a75068670eec5415MD51THUMBNAIL45106-216321-1-SM.pdf.jpg45106-216321-1-SM.pdf.jpgGenerated Thumbnailimage/jpeg6042https://repositorio.unal.edu.co/bitstream/unal/49323/2/45106-216321-1-SM.pdf.jpg3934c474bba455fa40864759b31f24f4MD52unal/49323oai:repositorio.unal.edu.co:unal/493232023-12-09 23:05:56.49Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
title Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
spellingShingle Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
Warfarina
validación
HPLC-DAD
preparación extemporánea
Warfarin
validation
HPLC-DAD
extemporaneous preparation
title_short Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
title_full Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
title_fullStr Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
title_full_unstemmed Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
title_sort Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation
dc.creator.fl_str_mv Berrío, Marcel
Trujillo, Mary
Vallejo, Bibiana Margarita
Barbosa, Hélber de Jesús
dc.contributor.author.spa.fl_str_mv Berrío, Marcel
Trujillo, Mary
Vallejo, Bibiana Margarita
Barbosa, Hélber de Jesús
dc.subject.proposal.spa.fl_str_mv Warfarina
validación
HPLC-DAD
preparación extemporánea
Warfarin
validation
HPLC-DAD
extemporaneous preparation
topic Warfarina
validación
HPLC-DAD
preparación extemporánea
Warfarin
validation
HPLC-DAD
extemporaneous preparation
description In this work was carried out the development and quantification of an analytical methodology for quantification of Warfarin Sodium in an extemporaneous suspension for hospital use. Evaluation of specificity was achieved against its excipients and degradation compounds. The selectivity was evaluated against the excipients and degradation compounds. Linearity studies were performed in a range of concentration between 0.05 to 0.15 mg/mL. Precision was evaluated in levels of repeatability and intermediate precision and accuracy in three concentration levels corresponding to the 75, 100 and 125%. The results show that the validation of the methodology is selective, linear, accurate and precise; therefore, it is reliable for use in the quantification of active warfarin sodium in the extemporaneous suspension.
publishDate 2013
dc.date.issued.spa.fl_str_mv 2013
dc.date.accessioned.spa.fl_str_mv 2019-06-29T08:35:26Z
dc.date.available.spa.fl_str_mv 2019-06-29T08:35:26Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/49323
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/42780/
http://bdigital.unal.edu.co/42780/2/
url https://repositorio.unal.edu.co/handle/unal/49323
http://bdigital.unal.edu.co/42780/
http://bdigital.unal.edu.co/42780/2/
dc.language.iso.spa.fl_str_mv spa
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dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/rccquifa/article/view/45106
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Ciencias Químico Farmacéuticas
Revista Colombiana de Ciencias Químico Farmacéuticas
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 122-133 0034-7418 1909-6356
dc.relation.references.spa.fl_str_mv Berrío, Marcel and Trujillo, Mary and Vallejo, Bibiana Margarita and Barbosa, Hélber de Jesús (2013) Development and validation of an analytical method by hplc-dad for quantification of warfarin sodium in an extemporaneous preparation. Revista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 122-133 0034-7418 1909-6356 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Revista Colombiana de Ciencias Químico Farmacéuticas
institution Universidad Nacional de Colombia
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