Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi

ilustraciones, diagramas, fotografías, gráficas, tablas

Autores:
Barreto Pulido, Wanner Ernesto
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/84346
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/84346
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::572 - Bioquímica
Metabolitos microbianos
Biosíntesis
Microbial metabolites
Biosynthesis
Dianthus caryophyllus L.
Fusarium oxysporum f. sp. dianthi
Fosfito de potasio
Metabolómica no dirigida
Perfilado metabólico
NPR1
P5CR
SAR
Untargeted metabolomics
Rights
openAccess
License
Reconocimiento 4.0 Internacional
id UNACIONAL2_9d0eeb568665660d637e1c6345caa3ff
oai_identifier_str oai:repositorio.unal.edu.co:unal/84346
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
dc.title.translated.eng.fl_str_mv Effect of the application of potassium phosphite on the biosynthesis of metabolites during the carnation (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
dc.title.translated.por.fl_str_mv Efeito da aplicação de fosfito de potássio na biossíntese de metabólitos durante o cravo (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianti
title Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
spellingShingle Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
570 - Biología::572 - Bioquímica
Metabolitos microbianos
Biosíntesis
Microbial metabolites
Biosynthesis
Dianthus caryophyllus L.
Fusarium oxysporum f. sp. dianthi
Fosfito de potasio
Metabolómica no dirigida
Perfilado metabólico
NPR1
P5CR
SAR
Untargeted metabolomics
title_short Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
title_full Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
title_fullStr Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
title_full_unstemmed Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
title_sort Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthi
dc.creator.fl_str_mv Barreto Pulido, Wanner Ernesto
dc.contributor.advisor.none.fl_str_mv Ardila Barrantes, Harold Duban
Coy Barrera, Ericsson David
dc.contributor.author.none.fl_str_mv Barreto Pulido, Wanner Ernesto
dc.contributor.researchgroup.spa.fl_str_mv Estudio de Actividades Metabolicas Vegetales
dc.contributor.orcid.spa.fl_str_mv Barreto Puldio, Wanner [0009-0004-1085-1041]
dc.contributor.cvlac.spa.fl_str_mv Barreto Pulido, Wanner [0001686629]
dc.subject.ddc.spa.fl_str_mv 570 - Biología::572 - Bioquímica
topic 570 - Biología::572 - Bioquímica
Metabolitos microbianos
Biosíntesis
Microbial metabolites
Biosynthesis
Dianthus caryophyllus L.
Fusarium oxysporum f. sp. dianthi
Fosfito de potasio
Metabolómica no dirigida
Perfilado metabólico
NPR1
P5CR
SAR
Untargeted metabolomics
dc.subject.lemb.spa.fl_str_mv Metabolitos microbianos
Biosíntesis
dc.subject.lemb.eng.fl_str_mv Microbial metabolites
Biosynthesis
dc.subject.proposal.other.fl_str_mv Dianthus caryophyllus L.
Fusarium oxysporum f. sp. dianthi
dc.subject.proposal.spa.fl_str_mv Fosfito de potasio
Metabolómica no dirigida
Perfilado metabólico
NPR1
P5CR
SAR
dc.subject.proposal.eng.fl_str_mv Untargeted metabolomics
description ilustraciones, diagramas, fotografías, gráficas, tablas
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-07-28T12:54:05Z
dc.date.available.none.fl_str_mv 2023-07-28T12:54:05Z
dc.date.issued.none.fl_str_mv 2023-07-19
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/84346
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/84346
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Reconocimiento 4.0 InternacionalDerechos reservados al autor, 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ardila Barrantes, Harold Duban3c0b4dc91cdf02792559faddcd37e2b3Coy Barrera, Ericsson David508c29a7d75f8c7bc49946bb300cabf3Barreto Pulido, Wanner Ernesto051b2fe4003d9fb688102b42f2cb239fEstudio de Actividades Metabolicas VegetalesBarreto Puldio, Wanner [0009-0004-1085-1041]Barreto Pulido, Wanner [0001686629]2023-07-28T12:54:05Z2023-07-28T12:54:05Z2023-07-19https://repositorio.unal.edu.co/handle/unal/84346Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías, gráficas, tablasEn la presente investigación se estudió el efecto que tiene la aplicación de una disolución al 3% de fosfito de potasio en la bioquímica de raíces de clavel durante la inducción de resistencia al patógeno Fusarium oxysporum f. sp. dianthi. Si bien las disoluciones de fosfito de potasio han sido usadas como fertilizantes, fungicidas sistémicos o activadores de defensa natural, para el caso del cultivo del clavel, su uso ha sido limitado y existen solo algunos reportes recientes sobre su papel como inductor de resistencia. Es por ello que, con el fin de profundizar en los procesos involucrados en la acción de este inductor, en la presente investigación, aplicando herramientas de análisis metabolómico no dirigido, se estudiaron los metabolitos que se acumulan en raíces de la planta por acción de este inductor de resistencia y se estudió la expresión de genes asociados con algunos de los metabolitos de interés. Para ello, en una primera etapa se llevó a cabo un ensayo in vivo donde se seleccionó la estrategia para la aplicación de la disolución de fosfito en el suelo. Posteriormente, se realizó la obtención del perfil metabólico mediante GC-MS, con previa derivatización con agente sililante; en este punto se identificaron metabolitos diferenciables para los tratamientos estudiados, entre los cuales se destacan algunos aminoácidos, carbohidratos y ácidos orgánicos, entre otros. Estos resultados indicaron que mecanismos como el metabolismo de aminoácidos, transporte de nitrógeno, metabolismo de carbohidratos y señalización en plantas, son importantes en la inducción de resistencia. Finalmente, en una tercera etapa se estudiaron los niveles transcripcionales de los genes p5cr y npr1, los cuales participan en la biosíntesis de prolina y en la activación de RSA (Resistencia Sistémica Adquirida), respectivamente. Se encontró que la aplicación del inductor generó un aumento en los niveles transcripcionales de dichos genes en la variedad susceptible, confirmando a nivel molecular la activación de las rutas estudiadas en este fenómeno biológico. Los hallazgos encontrados en esta investigación permiten sugerir que el uso de fosfito de potasio, en su rol de inductor de resistencia, potencializa la síntesis de metabolitos asociados a defensa vegetal, muy probablemente asociada a la activación de RSA dependiente de ácido salicílico. (Texto tomado de la fuente)In the present study, the effect of 3% potassium phosphite solution on the carnation root biochemistry during the resistance induction to the pathogen Fusarium oxysporum f. sp. dianthi was evaluated. Although potassium phosphite solutions have been used as fertilizers, systemic fungicides, or natural defense activators, in the case of carnation cultivation, their use has been limited, and there are only a few recent reports on their role as a resistance inducer. Therefore, to deepen the processes involved in the action of this inducer, in the present investigation, applying untargeted metabolomics-based analysis tools, those metabolites differentially accumulated in plant roots due to the action of this resistance inducer, as well as the expression of genes associated with some of the top-ranked metabolites, were studied. In this regard, the first stage involved an in vivo test where the strategy for applying the phosphite solution in the soil was selected. Subsequently, the metabolic profile was obtained by GC-MS, with prior derivatization with a silylating agent. At this point, differentiable metabolites were identified for the studied treatments, among which some amino acids, carbohydrates, and organic acids, among others, were statistically recognized. These results indicated that mechanisms such as amino acid metabolism, nitrogen transport, carbohydrate metabolism, and plant signaling are relevant for resistance induction. Finally, the third stage comprised the evaluation of transcriptional levels of the p5cr and npr1 genes, which participate in proline biosynthesis and ASR (Acquired Systemic Resistance) activation, respectively. It was found that the inducer application generated a transcriptional level increase in the test genes for the susceptible carnation variety, confirming at the molecular level the activation of those pathways studied in this biological phenomenon. 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(2018) ‘Análisis metabolómico no dirigido en dos líneas de conejos seleccionados de forma divergente para grasa intramuscular’, p. 74.Efecto de la aplicación de fosfito de potasio en la biosíntesis de metabolitos durante la interacción clavel (Dianthus caryophyllus L.) - Fusarium oxysporum f. sp. dianthiMincienciasEstudiantesInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84346/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1020796853.2023.pdf1020796853.2023.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf2796201https://repositorio.unal.edu.co/bitstream/unal/84346/2/1020796853.2023.pdf8c5360204fc87c4873a5016370b9d7e2MD52THUMBNAIL1020796853.2023.pdf.jpg1020796853.2023.pdf.jpgGenerated Thumbnailimage/jpeg5430https://repositorio.unal.edu.co/bitstream/unal/84346/3/1020796853.2023.pdf.jpge7067648469099f5477f445094e76d56MD53unal/84346oai:repositorio.unal.edu.co:unal/843462024-08-14 23:42:41.762Repositorio Institucional Universidad Nacional de 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