Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas
ilustraciones, tablas
- Autores:
-
Castro Moreno, Germán Andrés
- Tipo de recurso:
- Doctoral thesis
- Fecha de publicación:
- 2020
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/79731
- Palabra clave:
- 660 - Ingeniería química
Composición de los alimentos
Hidratos de carbono
Galactooligosacáridos
Proceso intensificado
Sistema acuoso de dos fases
Proceso asistido por microondas
Galactooligosaccharides
Intensified process
Aqueous two-phase system
Microwave-assisted process
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| dc.title.translated.eng.fl_str_mv |
Development of a process production-separation of galactooligosaccharides by a two-phase aqueous system microwave assisted |
| title |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| spellingShingle |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas 660 - Ingeniería química Composición de los alimentos Hidratos de carbono Galactooligosacáridos Proceso intensificado Sistema acuoso de dos fases Proceso asistido por microondas Galactooligosaccharides Intensified process Aqueous two-phase system Microwave-assisted process |
| title_short |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| title_full |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| title_fullStr |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| title_full_unstemmed |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| title_sort |
Desarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondas |
| dc.creator.fl_str_mv |
Castro Moreno, Germán Andrés |
| dc.contributor.advisor.none.fl_str_mv |
Serrato Bermúdez, Juan Carlos |
| dc.contributor.author.none.fl_str_mv |
Castro Moreno, Germán Andrés |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Procesos Químicos y Bioquímicos |
| dc.subject.ddc.spa.fl_str_mv |
660 - Ingeniería química |
| topic |
660 - Ingeniería química Composición de los alimentos Hidratos de carbono Galactooligosacáridos Proceso intensificado Sistema acuoso de dos fases Proceso asistido por microondas Galactooligosaccharides Intensified process Aqueous two-phase system Microwave-assisted process |
| dc.subject.lemb.none.fl_str_mv |
Composición de los alimentos Hidratos de carbono |
| dc.subject.proposal.spa.fl_str_mv |
Galactooligosacáridos Proceso intensificado Sistema acuoso de dos fases Proceso asistido por microondas |
| dc.subject.proposal.eng.fl_str_mv |
Galactooligosaccharides Intensified process Aqueous two-phase system Microwave-assisted process |
| description |
ilustraciones, tablas |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2021-06-25T21:41:59Z |
| dc.date.available.none.fl_str_mv |
2021-06-25T21:41:59Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Doctorado |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_db06 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TD |
| format |
http://purl.org/coar/resource_type/c_db06 |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/79731 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/79731 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial-SinDerivadas 4.0 InternacionalDerechos Reservados al Autor, 2020http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Serrato Bermúdez, Juan Carlos2cec2f7270f1d605a516d2e2beace5a2Castro Moreno, Germán Andrés155e80301cc6bb48cd5235cb60ae9e70Grupo de Investigación en Procesos Químicos y Bioquímicos2021-06-25T21:41:59Z2021-06-25T21:41:59Z2020https://repositorio.unal.edu.co/handle/unal/79731Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, tablasLos GOS (Galactooligosacáridos) son carbohidratos con una molécula de glucosa unida a 2-5 moléculas de galactosa. Los GOS son prebióticos y se obtienen industrialmente mediante reacciones enzimáticas. Los GOS son producidos a partir de lactosa, ya sea en estado puro o haciendo parte del suero de quesería, el cual debe presentar un contenido de lactosa superior al 70% (m/m). La reacción de hidrólisis rompe la lactosa en glucosa y galactosa, que reacciona para formar GOS, a través de la reacción de transgalactosilación, estas dos reacciones suceden simultáneamente, ya que una se nutre de la otra. La presencia de estos monosacáridos inhibe la última reacción y disminuye la producción de GOS. Los sistemas acuosos de dos fases (SADF) pueden usarse para separar una mezcla de sustancias de acuerdo con su afinidad preferencial por una de las dos fases en este sistema. Estos sistemas están formados por dos polímeros o una sal y un polímero disueltos en agua en una concentración específica para producir la separación de fases. Hay muchos factores que afectan esta operación, como el pH, la temperatura, el polímero, la concentración de sales y sustancias, el peso molecular del polímero y la relación polímero / sal. El SADF puede usarse para separar los monosacáridos inhibidores de la mezcla de GOS. La irradiación de microondas ha mostrado un efecto positivo en la separación por SADF y se ha utilizado para mejorar la reacción de producción de GOS a partir de lactosa. El SADF se utilizó en este trabajo para producir y separar GOS simultáneamente. El medio de reacción estaba formado por lactosa a diferentes concentraciones, polietilenglicol (PEG) y citrato de sodio para formar el SADF, pH de 4.5 a 5.5, las temperaturas probadas estuvieron entre 40 y 60 ° C. Se evaluó el efecto de la irradiación de microondas sobre la reacción, la separación por SADF y la reacción - separación simultáneas. Se establecieron todas las condiciones del SADF que produjeron resultados optimizados, que se expresaron como coeficiente de partición, rendimiento de separación, selectividad para GOS y rendimiento de reacción. La selectividad para GOS aumentó más del 40%, en relación con el medio convencional (buffer + lactosa), el rendimiento de la reacción no cambió estadísticamente. La separación de la mezcla de carbohidratos fue de acuerdo con su peso molecular, las sustancias más pesadas (GOS) se mantuvieron principalmente en la fase inferior (citrato de sodio), mientras que los monosacáridos migraron en mayor medida a la fase superior (PEG). Los resultados optimizados de reacción, separación y separación simultánea de reacción se probaron en irradiación de microondas. El rendimiento, la producción, la selectividad y la productividad disminuyeron con la acción de microondas. (Texto tomado de la fuente).GOS are carbohydrates with a glucose molecule linked to 2-5 molecules of galactose. GOS are prebiotics and are industrially obtained by enzymatic reactions. GOS are produced from lactose, it might be pure or as part of cheese whey with a lactose content higher than 70%. Hydrolysis reaction breaks lactose into glucose and galactose, which reacts to form GOS, through transgalactosylation reaction; those reactions occur simultaneously because each one depends of the other. Monosaccharides inhibit transgalactosylation reaction and decrease the GOS production. Aqueous two phase system (ATPS) could be used to separate a mixture of substances according to its preferential affinity to one of the two phases in this system, it is formed by two polymers or a salt and a polymer dissolved in water upon a specific concentration to produce phase separation. There are many factors affecting this operation like pH, temperature, polymer, salt and substances concentration, molecular weight of polymer and polymer / salt ratio, ATPS could be used to separate inhibitor monosaccharides, from GOS mixture. Microwave irradiation has shown a positive effect on ATPS separation and has been used to improve GOS production reaction from lactose. ATPS was used in this work to produce and separate galactooligosaccharides simultaneously. Reaction medium was formed by lactose at different concentrations, polyethyleneglycol (PEG) and sodium citrate to form ATPS, pH of 4.5 – 5.5, temperatures tested were between 40 - 60°C. Was evaluated the effect of microwave irradiation on reaction, ATPS separation and simultaneous reactions-separation conditions. Were stablished all conditions in ATPS that produced optimized results, which were expressed as partition coefficient, separation yield, selectivity to GOS and reaction yield. Selectivity to GOS raise up more than 40%, relative to conventional medium, reaction yield did not change statistically. Separation of carbohydrates mixture is according to its molecular weight, heaviest substances (GOS) kept mainly in the bottom phase (sodium citrate), while monosaccharides kept in top phase (PEG) mainly. Optimized results of reaction, separation and simultaneous reaction-separation were tested into microwave irradiation. Yield, production, selectivity and productivity were increased with microwave action. (Texto tomado de la fuente).DoctoradoDoctor en Ingeniería - Ingeniería QuímicaBioprocesos168 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ingeniería - Doctorado en Ingeniería - Ingeniería QuímicaDepartamento de Ingeniería Química y AmbientalFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá660 - Ingeniería químicaComposición de los alimentosHidratos de carbonoGalactooligosacáridosProceso intensificadoSistema acuoso de dos fasesProceso asistido por microondasGalactooligosaccharidesIntensified processAqueous two-phase systemMicrowave-assisted processDesarrollo de un proceso de producción-separación de galactooligosacáridos mediante un sistema acuoso de dos fases asistido por microondasDevelopment of a process production-separation of galactooligosaccharides by a two-phase aqueous system microwave assistedTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAdam, D. 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Consultado 03/03/2015.GeneralORIGINALPhD Tesis Germán Castro Moreno Final.pdfPhD Tesis Germán Castro Moreno Final.pdfTesis de Doctorado en Ingeniería - Ingeniería Químicaapplication/pdf2070707https://repositorio.unal.edu.co/bitstream/unal/79731/5/PhD%20Tesis%20Germ%c3%a1n%20Castro%20Moreno%20Final.pdf2efa821ab27dab1f1e77298fb22ef09fMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79731/3/license.txtcccfe52f796b7c63423298c2d3365fc6MD53THUMBNAILPhD Tesis Germán Castro Moreno Final.pdf.jpgPhD Tesis Germán Castro Moreno Final.pdf.jpgGenerated Thumbnailimage/jpeg5559https://repositorio.unal.edu.co/bitstream/unal/79731/6/PhD%20Tesis%20Germ%c3%a1n%20Castro%20Moreno%20Final.pdf.jpg513236a69f3423ae1e245e8e1277f12bMD56unal/79731oai:repositorio.unal.edu.co:unal/797312024-07-23 23:33:38.694Repositorio Institucional Universidad Nacional de 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