Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)

ilustraciones, diagramas

Autores:: Fajardo Figueroa, Lina Maria

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
dc.title.translated.eng.fl_str_mv	Thermodynamic and metabolic analysis of a biological system: insect cells Spodoptera frugiperda (sf9) and their infection with a recombinant virus Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
title	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
spellingShingle	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1) 660 - Ingeniería química Biotecnología celular en insectos Insect cell biotechnology Spodoptera frugiperda Proteínas recombinantes Baculovirus Termodinámica Tiempo característico Spodoptera frugiperda Protein production Baculovirus Thermodynamic Characteristic time
title_short	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
title_full	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
title_fullStr	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
title_full_unstemmed	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
title_sort	Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)
dc.creator.fl_str_mv	Fajardo Figueroa, Lina Maria
dc.contributor.advisor.none.fl_str_mv	Suárez Méndez, Camilo Alberto
dc.contributor.author.none.fl_str_mv	Fajardo Figueroa, Lina Maria
dc.contributor.researchgroup.spa.fl_str_mv	Bioprocesos y Flujos Reactivos
dc.subject.ddc.spa.fl_str_mv	660 - Ingeniería química
topic	660 - Ingeniería química Biotecnología celular en insectos Insect cell biotechnology Spodoptera frugiperda Proteínas recombinantes Baculovirus Termodinámica Tiempo característico Spodoptera frugiperda Protein production Baculovirus Thermodynamic Characteristic time
dc.subject.lemb.spa.fl_str_mv	Biotecnología celular en insectos
dc.subject.lemb.eng.fl_str_mv	Insect cell biotechnology
dc.subject.proposal.spa.fl_str_mv	Spodoptera frugiperda Proteínas recombinantes Baculovirus Termodinámica Tiempo característico
dc.subject.proposal.eng.fl_str_mv	Spodoptera frugiperda Protein production Baculovirus Thermodynamic Characteristic time
description	ilustraciones, diagramas
publishDate	2022
dc.date.issued.none.fl_str_mv	2022-10-10
dc.date.accessioned.none.fl_str_mv	2023-01-25T21:41:23Z
dc.date.available.none.fl_str_mv	2023-01-25T21:41:23Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/83129
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/83129 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.indexed.spa.fl_str_mv	RedCol LaReferencia
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Medellín - Minas - Maestría en Ingeniería - Ingeniería Química
dc.publisher.faculty.spa.fl_str_mv	Facultad de Minas
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
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institution	Universidad Nacional de Colombia
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spelling	Atribución-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Suárez Méndez, Camilo Alberto997598635d63c1552bb6d43d1091be66Fajardo Figueroa, Lina Mariadfdd85fc17e83126547e154c531c9845Bioprocesos y Flujos Reactivos2023-01-25T21:41:23Z2023-01-25T21:41:23Z2022-10-10https://repositorio.unal.edu.co/handle/unal/83129Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasEn el presente trabajo se aborda el análisis de la producción de proteínas recombinantes con interés bio-farmacéutico a través de la tecnología de expresión en células de insecto mediante la infección con baculovirus recombinantes. Esta es una estrategia ampliamente utilizada que aún tiene desafíos en el diseño, modelamiento y entendimiento (Monteiro, 2015). En primer lugar, se analiza bajo una perspectiva termodinámica de caja negra la etapa de crecimiento celular encontrando que su modelamiento es posible a través de una metodología, dirigida principalmente a microorganismos, la cual se puede adaptar a eucariotas con especial consideración en la definición y diferenciación de la fuente de energía, carbono y nitrógeno. En particular, cuando se considera en el análisis una fuente de nitrógeno orgánica, la cual induce un sobreflujo de electrones que pueden terminar en biomasa o en subrpoductos carbonados como lactato. Aunque no fue posible reproducir los escenarios planteados en un modelo metabólico, mediante el análisis metabólico se identificó la importancia de considerar las rutas de asimilación de nitrógeno (orgánico e inorgánico) y el uso de métodos estadísticos como herramienta para la mejora de estos procesos. Finalmente, basado en el análisis de tiempos característicos derivados de cinéticas reportadas para la etapa de infección y producción en la literatura, se encontró que el evento celular que principalmente afecta la homogeneidad y la productividad de las proteínas recombinantes es el evento de unión virus-célula ya que su tiempo característico puede variar considerablemente y afectar otros subprocesos. Además, se encontró que, en la producción de proteínas complejas a nivel intracelular, su expresión se ve favorecida en linajes celulares con velocidades de internalización más bajas. (Texto tomado de la fuente)Here, the analysis of producing recombinant proteins of bio-pharmaceutical interest based on its expression in insect cells through infection with recombinant baculoviruses was addressed. This approach is a widely used strategy that still has challenges in design, modeling and understanding (Monteiro, 2015). First, the cell growth stage was analyzed from a black box thermodynamic perspective, showing that this modeling approach may be used to this biological model, even though it was formerly meant for microorganisms. The methodology can be applied to eukaryotes having special consideration on the definition and differentiation of the energy, carbon, and nitrogen sources. When an organic nitrogen source is considered in the analysis, it induces an overflow of electrons that can end up in biomass or in carbon by-products such as lactate. Although it was not possible to reproduce some scenarios proposed in a metabolic model, the metabolic analysis did show the importance of considering nitrogen assimilation routes (organic and inorganic) and the use of statistical methods as a tool to improve these processes. Finally, based on the analysis of characteristic times derived from kinetics reported for the infection and production stage in literature, it was found that the virus-cell union is the cellular event that mainly affects homogeneity and productivity of recombinant proteins since its characteristic time can vary considerably affecting other subsequent subprocesses. In addition, it is shown that their expression is favored in cell lines with lower internalization rates in the production of complex proteins at the intracellular level.MaestríaMagíster en Ingeniería - Ingeniería QuímicaBioprocess Intensification and Rational DesignÁrea curricular de Ingeniería Química e Ingeniería de Petróleosxx, 88 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Minas - Maestría en Ingeniería - Ingeniería QuímicaFacultad de MinasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería químicaBiotecnología celular en insectosInsect cell biotechnologySpodoptera frugiperdaProteínas recombinantesBaculovirusTermodinámicaTiempo característicoSpodoptera frugiperdaProtein productionBaculovirusThermodynamicCharacteristic timeAnálisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)Thermodynamic and metabolic analysis of a biological system: insect cells Spodoptera frugiperda (sf9) and their infection with a recombinant virus Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferenciaAdnan, N. 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The University of Queensland.EstudiantesInvestigadoresMaestrosProveedores de ayuda financiera para estudiantesPúblico generalORIGINAL1144089629.2022.pdf.pdf1144089629.2022.pdf.pdfTesis de Maestría en Ingeniería - Ingeniería Químicaapplication/pdf2741329https://repositorio.unal.edu.co/bitstream/unal/83129/2/1144089629.2022.pdf.pdf4b94c11e7736c81dba9143343acf360dMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83129/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL1144089629.2022.pdf.pdf.jpg1144089629.2022.pdf.pdf.jpgGenerated Thumbnailimage/jpeg6218https://repositorio.unal.edu.co/bitstream/unal/83129/3/1144089629.2022.pdf.pdf.jpg9bec8c314cd23abe069755404d529d1bMD53unal/83129oai:repositorio.unal.edu.co:unal/831292023-08-13 23:05:41.811Repositorio Institucional Universidad Nacional de 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Análisis termodinámico y metabólico de un sistema biológico células de insecto Spodoptera frugiperda (sf9) y su infección con un virus recombinante Autographa californica nucleopolyhedrovirus (BvGCN4-NA1)

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